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The map label for this gene is nusA [H]

Identifier: 239617901

GI number: 239617901

Start: 1641126

End: 1642154

Strand: Reverse

Name: nusA [H]

Synonym: Kole_1529

Alternate gene names: 239617901

Gene position: 1642154-1641126 (Counterclockwise)

Preceding gene: 239617902

Following gene: 239617900

Centisome position: 71.33

GC content: 44.12

Gene sequence:

>1029_bases
ATGAATCTCAATCTTCTGGAAGCGCTGGAACAACTTGAAGAAGAAAAAAATATTAAAAAAGACGAAGTTATCAGTATTCT
TGAAAAAGCACTCCAGAGCGCTTATAGAAAAAACTTTGGCACCGAAAATGAGGTTGAAGTTAGGATAGACCGTCTCACCG
GTGATATCGCCATATATGAAAAATTAAAGGTTGTTGACGAAGTTGAGAACCCTTATCGTGAGATCACTCTTTCAGAAGCT
AAGAAGATAGATTCTGATGCTGAGATCGATGGTTATGTCTACAGAAAGCTCAATATAAAGAAATTCAAGAGAATAGCCGC
TCAAACGGCCAGACAGGTTTTGATTCAGAAAATTCGGGAAATGGAGAAGGAAAATCTTTTCCAGACGTATTCTGCACTTA
AAGGTTCCGTCACCACAGCAGAAATACTCAGAGTAACCGAAAACTGGGCTGACATAAGAATAGGAAAACTTGAATCAAGG
CTTCCTACCCGCGAAATGATTCCCGGCGAGAGGCTCAGGGCAGGCAGTTTTATAAAGGTATTCGTAGTAGATGTCGTCAG
AACAACGAAAGGTCCAAAGATTCTCGTTACCAGAAAAGGGACAGAGTTCGTAGTGGAGTTGTTAAAACTCCAGGTTCCAG
AAATCGAGAGCGGTGATGTGGTGGTTAAAGCCGTCGCAAGGGAAGAAGGTATCAGGACAAAGGTTGCCGTTGCATCTACC
AATCCCAAAGTAGATCCCATAGGAGCCTGCATTGGTGAAGGCGGGGTCAGAATCGCAGAGGTTCTGCGCGAAATAAGGCC
TGAGAAGGTTGACATATTAAAATGGAGCGACGATCCGGCAGAATTCGTCGGTAATGCAATAGCTCCAGCCACAGCCGTGG
AGGTTAAAATAGTCAGTTCCGCAAACCGTGAGGCCGTTGTCTATGTTTCTCCCACACAGCTCTCACTCGCAATTGGAAAA
GGTGGTCAAAATGCCAGGCTTGCAGCAAAATTAACCGGCTGGAAGGTTGACATCAAACCTTTGATGTAG

Upstream 100 bases:

>100_bases
CATAATCGAGGCTGACAGCAACAGGACATTTGAAGTTGAATACAGAATGATAAAGAAAGCAAATTTGAGACTTGAACGGT
AATCTTGGGAGGTGCAGAAG

Downstream 100 bases:

>100_bases
ATATTTTGTAACATATTACCTACTCTCTGTAAATGAACTCATGTAAAATGAATCTGATGATCCTTATTCACCAACAGTTT
TGGCAGGGAGGTTTTGAAAG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNLNLLEALEQLEEEKNIKKDEVISILEKALQSAYRKNFGTENEVEVRIDRLTGDIAIYEKLKVVDEVENPYREITLSEA
KKIDSDAEIDGYVYRKLNIKKFKRIAAQTARQVLIQKIREMEKENLFQTYSALKGSVTTAEILRVTENWADIRIGKLESR
LPTREMIPGERLRAGSFIKVFVVDVVRTTKGPKILVTRKGTEFVVELLKLQVPEIESGDVVVKAVAREEGIRTKVAVAST
NPKVDPIGACIGEGGVRIAEVLREIRPEKVDILKWSDDPAEFVGNAIAPATAVEVKIVSSANREAVVYVSPTQLSLAIGK
GGQNARLAAKLTGWKVDIKPLM

Sequences:

>Translated_342_residues
MNLNLLEALEQLEEEKNIKKDEVISILEKALQSAYRKNFGTENEVEVRIDRLTGDIAIYEKLKVVDEVENPYREITLSEA
KKIDSDAEIDGYVYRKLNIKKFKRIAAQTARQVLIQKIREMEKENLFQTYSALKGSVTTAEILRVTENWADIRIGKLESR
LPTREMIPGERLRAGSFIKVFVVDVVRTTKGPKILVTRKGTEFVVELLKLQVPEIESGDVVVKAVAREEGIRTKVAVAST
NPKVDPIGACIGEGGVRIAEVLREIRPEKVDILKWSDDPAEFVGNAIAPATAVEVKIVSSANREAVVYVSPTQLSLAIGK
GGQNARLAAKLTGWKVDIKPLM
>Mature_342_residues
MNLNLLEALEQLEEEKNIKKDEVISILEKALQSAYRKNFGTENEVEVRIDRLTGDIAIYEKLKVVDEVENPYREITLSEA
KKIDSDAEIDGYVYRKLNIKKFKRIAAQTARQVLIQKIREMEKENLFQTYSALKGSVTTAEILRVTENWADIRIGKLESR
LPTREMIPGERLRAGSFIKVFVVDVVRTTKGPKILVTRKGTEFVVELLKLQVPEIESGDVVVKAVAREEGIRTKVAVAST
NPKVDPIGACIGEGGVRIAEVLREIRPEKVDILKWSDDPAEFVGNAIAPATAVEVKIVSSANREAVVYVSPTQLSLAIGK
GGQNARLAAKLTGWKVDIKPLM

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=339, Percent_Identity=34.8082595870207, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 38121; Mature: 38121

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNLLEALEQLEEEKNIKKDEVISILEKALQSAYRKNFGTENEVEVRIDRLTGDIAIYE
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHH
KLKVVDEVENPYREITLSEAKKIDSDAEIDGYVYRKLNIKKFKRIAAQTARQVLIQKIRE
HHHHHHHHCCHHHHEEHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
MEKENLFQTYSALKGSVTTAEILRVTENWADIRIGKLESRLPTREMIPGERLRAGSFIKV
HHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHEEEECHHHCCCHHHCCCCCCCCCCCCHHH
FVVDVVRTTKGPKILVTRKGTEFVVELLKLQVPEIESGDVVVKAVAREEGIRTKVAVAST
HHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCEEEEEEECC
NPKVDPIGACIGEGGVRIAEVLREIRPEKVDILKWSDDPAEFVGNAIAPATAVEVKIVSS
CCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCCCCEEEEEEEEC
ANREAVVYVSPTQLSLAIGKGGQNARLAAKLTGWKVDIKPLM
CCCCEEEEECCCEEEEEECCCCCCCEEEEEECCEEEEEECCC
>Mature Secondary Structure
MNLNLLEALEQLEEEKNIKKDEVISILEKALQSAYRKNFGTENEVEVRIDRLTGDIAIYE
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHH
KLKVVDEVENPYREITLSEAKKIDSDAEIDGYVYRKLNIKKFKRIAAQTARQVLIQKIRE
HHHHHHHHCCHHHHEEHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
MEKENLFQTYSALKGSVTTAEILRVTENWADIRIGKLESRLPTREMIPGERLRAGSFIKV
HHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHEEEECHHHCCCHHHCCCCCCCCCCCCHHH
FVVDVVRTTKGPKILVTRKGTEFVVELLKLQVPEIESGDVVVKAVAREEGIRTKVAVAST
HHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCEEEEEEECC
NPKVDPIGACIGEGGVRIAEVLREIRPEKVDILKWSDDPAEFVGNAIAPATAVEVKIVSS
CCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCCCCEEEEEEEEC
ANREAVVYVSPTQLSLAIGKGGQNARLAAKLTGWKVDIKPLM
CCCCEEEEECCCEEEEEECCCCCCCEEEEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]