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The map label for this gene is rlmI [H]

Identifier: 239617895

GI number: 239617895

Start: 1634583

End: 1635764

Strand: Reverse

Name: rlmI [H]

Synonym: Kole_1523

Alternate gene names: 239617895

Gene position: 1635764-1634583 (Counterclockwise)

Preceding gene: 239617896

Following gene: 239617894

Centisome position: 71.05

GC content: 41.2

Gene sequence:

>1182_bases
GTGCCAGAATACAGAGTAAAACTAAAAAAAGAAATAAAAAGACGAATTGCCTTCGGCCATCCCTGGATATACGAAAACGA
GATAGCAGAAGAGCCTTCATGTGAAAACGGTTCTCTGGTCAAAGTATTCACTTACTCCGGTCAATTTGTGGGTAAAGGTT
ATTACAACAGGAATTCTCTGATACGCGTCAGGCTTTTGACCAGGAAGAATGTAAACATAGACAGAAAGTTTTTCAAAGAC
AAGATACAAAGGGCTTATTCACTCAGAACCCGCTGGCTCAAAGAAAAGGGAGCTTTCAGATTGATTTACAGTGAAGCTGA
TGGATTGCCCGGGCTTATAGTGGATAAATTCGCCGATTTTTTCGTTGTTCAGATCTCCACCCTCGGTATGAGTAAATTCA
AAGAAACCATAGTGAACGTCCTGATTGAACTCTTTAATCCAAAAGGGATCTACGAAAAATCTGAGGGCAGCTTCATTGCC
AAAGAAGGAATAGAACCCTATTCTGGATGGCTTTATGGAAACGGGCCAAATTTAATACCCTTTAAGCTACATGAAATCAC
CTTCCTTGCCGATCTACACGGACAAAAAACAGGGTTTTTTCTCGATCAGAGATTCAACGCTGAAAATCTGTCAGAGTTTT
CCACAGACAGAAGGGTTCTCGATCTTTTCTCATACACCGGTAACTTCGCATTCCATTTATTGAAAGGTGGAGCACGGGAA
GCGATCCTCGTTGATTATTCTGAACGCGCATTAGCGGTTGCTGAAGAGATAGCAAGGATAAACAGCTTCAAAGAAAAAAT
TCGAACGATAAACGCAAATTCCTTTGATTTTATAAGAAATGTGAAGATAGGGGAATTCGAGTTGATAGTCATCGACCCGC
CAGCGATGTTGAAATCAGCAACCGCAAAGCACAACGCGCTAAGAGCCTATAAAGAACTCAACCTGAGAGCTATTCGTTCG
TTGAAAGATGGAGGAATCCTTGCAACATCCTCTTGTACACAGCTCATTCGCGAATCCGAATGGCTCGAAAAGATAACGGA
AGCATTCAAAGATTCAAAAAAGATTGGCAAACTACTGTTTTCAGCCGGGCAACCTTTCGATCATCCGGCTCTAACAAGTA
TGCACGAATCAAACTATCTAAAGTTTAGAAGCTATATTGTAAGCAGTGTTAGCGAATTTTGA

Upstream 100 bases:

>100_bases
AAAGCTGGGCGGAAAAAGCTGCCAGAGCTCCACACAATGCAAGAACCCCGGGTGAGTTCGATGTCGAGTTTTATAGAAAC
CTCTATAAAAAGGTGAGAAA

Downstream 100 bases:

>100_bases
TTTACCTTGCTACAAATGATGTATAATTCTATTAAAGAGTTATTCTCTGGAGGTAGGTACCCATGGCCAAAGTTTATGTC
GTTACGTCAGGAAAGGGTGG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MPEYRVKLKKEIKRRIAFGHPWIYENEIAEEPSCENGSLVKVFTYSGQFVGKGYYNRNSLIRVRLLTRKNVNIDRKFFKD
KIQRAYSLRTRWLKEKGAFRLIYSEADGLPGLIVDKFADFFVVQISTLGMSKFKETIVNVLIELFNPKGIYEKSEGSFIA
KEGIEPYSGWLYGNGPNLIPFKLHEITFLADLHGQKTGFFLDQRFNAENLSEFSTDRRVLDLFSYTGNFAFHLLKGGARE
AILVDYSERALAVAEEIARINSFKEKIRTINANSFDFIRNVKIGEFELIVIDPPAMLKSATAKHNALRAYKELNLRAIRS
LKDGGILATSSCTQLIRESEWLEKITEAFKDSKKIGKLLFSAGQPFDHPALTSMHESNYLKFRSYIVSSVSEF

Sequences:

>Translated_393_residues
MPEYRVKLKKEIKRRIAFGHPWIYENEIAEEPSCENGSLVKVFTYSGQFVGKGYYNRNSLIRVRLLTRKNVNIDRKFFKD
KIQRAYSLRTRWLKEKGAFRLIYSEADGLPGLIVDKFADFFVVQISTLGMSKFKETIVNVLIELFNPKGIYEKSEGSFIA
KEGIEPYSGWLYGNGPNLIPFKLHEITFLADLHGQKTGFFLDQRFNAENLSEFSTDRRVLDLFSYTGNFAFHLLKGGARE
AILVDYSERALAVAEEIARINSFKEKIRTINANSFDFIRNVKIGEFELIVIDPPAMLKSATAKHNALRAYKELNLRAIRS
LKDGGILATSSCTQLIRESEWLEKITEAFKDSKKIGKLLFSAGQPFDHPALTSMHESNYLKFRSYIVSSVSEF
>Mature_392_residues
PEYRVKLKKEIKRRIAFGHPWIYENEIAEEPSCENGSLVKVFTYSGQFVGKGYYNRNSLIRVRLLTRKNVNIDRKFFKDK
IQRAYSLRTRWLKEKGAFRLIYSEADGLPGLIVDKFADFFVVQISTLGMSKFKETIVNVLIELFNPKGIYEKSEGSFIAK
EGIEPYSGWLYGNGPNLIPFKLHEITFLADLHGQKTGFFLDQRFNAENLSEFSTDRRVLDLFSYTGNFAFHLLKGGAREA
ILVDYSERALAVAEEIARINSFKEKIRTINANSFDFIRNVKIGEFELIVIDPPAMLKSATAKHNALRAYKELNLRAIRSL
KDGGILATSSCTQLIRESEWLEKITEAFKDSKKIGKLLFSAGQPFDHPALTSMHESNYLKFRSYIVSSVSEF

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=387, Percent_Identity=32.5581395348837, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1787181, Length=227, Percent_Identity=29.0748898678414, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 45075; Mature: 44944

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEYRVKLKKEIKRRIAFGHPWIYENEIAEEPSCENGSLVKVFTYSGQFVGKGYYNRNSL
CCCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCEEEEEEECCCEEECCEECCCCE
IRVRLLTRKNVNIDRKFFKDKIQRAYSLRTRWLKEKGAFRLIYSEADGLPGLIVDKFADF
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
FVVQISTLGMSKFKETIVNVLIELFNPKGIYEKSEGSFIAKEGIEPYSGWLYGNGPNLIP
HEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHCCCCCCCCEEECCCCCCCE
FKLHEITFLADLHGQKTGFFLDQRFNAENLSEFSTDRRVLDLFSYTGNFAFHLLKGGARE
EEEHHEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
AILVDYSERALAVAEEIARINSFKEKIRTINANSFDFIRNVKIGEFELIVIDPPAMLKSA
EEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEECCHHHHHHH
TAKHNALRAYKELNLRAIRSLKDGGILATSSCTQLIRESEWLEKITEAFKDSKKIGKLLF
HHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGQPFDHPALTSMHESNYLKFRSYIVSSVSEF
HCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PEYRVKLKKEIKRRIAFGHPWIYENEIAEEPSCENGSLVKVFTYSGQFVGKGYYNRNSL
CCHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCEEEEEEECCCEEECCEECCCCE
IRVRLLTRKNVNIDRKFFKDKIQRAYSLRTRWLKEKGAFRLIYSEADGLPGLIVDKFADF
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
FVVQISTLGMSKFKETIVNVLIELFNPKGIYEKSEGSFIAKEGIEPYSGWLYGNGPNLIP
HEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHCCCCCCCCEEECCCCCCCE
FKLHEITFLADLHGQKTGFFLDQRFNAENLSEFSTDRRVLDLFSYTGNFAFHLLKGGARE
EEEHHEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
AILVDYSERALAVAEEIARINSFKEKIRTINANSFDFIRNVKIGEFELIVIDPPAMLKSA
EEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEECCHHHHHHH
TAKHNALRAYKELNLRAIRSLKDGGILATSSCTQLIRESEWLEKITEAFKDSKKIGKLLF
HHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGQPFDHPALTSMHESNYLKFRSYIVSSVSEF
HCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA