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The map label for this gene is gyaR [H]

Identifier: 239617273

GI number: 239617273

Start: 928346

End: 929299

Strand: Reverse

Name: gyaR [H]

Synonym: Kole_0882

Alternate gene names: 239617273

Gene position: 929299-928346 (Counterclockwise)

Preceding gene: 239617274

Following gene: 239617272

Centisome position: 40.37

GC content: 41.82

Gene sequence:

>954_bases
TTGGAAAAGATTTTTGTAACCTATAAGATTCCGGAAATAGGATTGAAACTCCTGAGCAAATTCAATGTTGAGGTCAACGA
GGAAGATAGAACCCTTTCGAAAAAAGAGATAATAGAAAGGGCACAAGATGCGACCGCTCTGGTAACACTTTTATCCGATA
ACATAGATGCTGAAATAATAAACGCACTTCCAAGACTCAAAATAATTGCCAATTATGCTGTTGGATTCAATAATATTGAT
ATAGAAGCTGCGAAAGCAAAAGGAGTAATCGTTACAAACACGCCGGATATTCTTACAGATGCCTCTGCCGATCTTGCAAT
GGCTCTATTGCTTGCCACTGCTAGAAGGATTGTTGAAGCAGATAAATTTGTAAGGAAAGGCCTCTTCGAGGGATGGAAAC
CGGAGCTGTTCCTCGGAATTGAGCTGAACGGAAAAACTCTCGGGATAATTGGACTTGGAAGGATAGGAAAAGCTGTTGCT
AAGCGGGCTCAAGCTTTTGGGATGAAAGTTATATATCATAATAGAAGGCCTCTAACAAGTGAAGAGGAAAAAAATCTTGA
TGTCGAGTACAGAAGTTTGGAACAGCTGTTGAAGGAATCTGATTTTATATCTCTTCACGTACCTCTGACCAGTGAAACTT
ATCATCTCCTTAGCAGAAGTAAATTGAAGCTCATGAAGCCATCAGCGGTACTGATTAACACATCGAGAGGCGCCGTTGTG
GACGAAGAAGCTTTGATAGAGTTTTTGCAGCAGGGTAAAATAGCTGCTGCGGGACTGGATGTTTATGAGAACGAACCTGA
GGTTCCGTATGCTCTTAAGGAACTTGACAACGTGGTTTTACTGCCGCACATCGGTTCGGCAACAGTAGAAACCAGAAACA
ACATGGCTGTTCTCGTAGCAAAAAACGTTCTCGCGGTTCTCGAAGGAAAGAAACCTTTGACGCCTGTTTATTGA

Upstream 100 bases:

>100_bases
GCCGGTCGTAACAATCCCGAAGGGATTCGTGCCGCAAAGTGGCTTTTTCGGACTCTCGTCCTCTCGGTTCTCGGATTTCT
TAGGAAAGGAGGTAGTTCCC

Downstream 100 bases:

>100_bases
TGTCGCCAAATATTTAAGGTGATAACGTGCAAAAAAAGTATTTTTACGACAATCCAGACTTTGAATTTACGATAACGAGA
GAGATAAGCGGTTTCACGTT

Product: D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MEKIFVTYKIPEIGLKLLSKFNVEVNEEDRTLSKKEIIERAQDATALVTLLSDNIDAEIINALPRLKIIANYAVGFNNID
IEAAKAKGVIVTNTPDILTDASADLAMALLLATARRIVEADKFVRKGLFEGWKPELFLGIELNGKTLGIIGLGRIGKAVA
KRAQAFGMKVIYHNRRPLTSEEEKNLDVEYRSLEQLLKESDFISLHVPLTSETYHLLSRSKLKLMKPSAVLINTSRGAVV
DEEALIEFLQQGKIAAAGLDVYENEPEVPYALKELDNVVLLPHIGSATVETRNNMAVLVAKNVLAVLEGKKPLTPVY

Sequences:

>Translated_317_residues
MEKIFVTYKIPEIGLKLLSKFNVEVNEEDRTLSKKEIIERAQDATALVTLLSDNIDAEIINALPRLKIIANYAVGFNNID
IEAAKAKGVIVTNTPDILTDASADLAMALLLATARRIVEADKFVRKGLFEGWKPELFLGIELNGKTLGIIGLGRIGKAVA
KRAQAFGMKVIYHNRRPLTSEEEKNLDVEYRSLEQLLKESDFISLHVPLTSETYHLLSRSKLKLMKPSAVLINTSRGAVV
DEEALIEFLQQGKIAAAGLDVYENEPEVPYALKELDNVVLLPHIGSATVETRNNMAVLVAKNVLAVLEGKKPLTPVY
>Mature_317_residues
MEKIFVTYKIPEIGLKLLSKFNVEVNEEDRTLSKKEIIERAQDATALVTLLSDNIDAEIINALPRLKIIANYAVGFNNID
IEAAKAKGVIVTNTPDILTDASADLAMALLLATARRIVEADKFVRKGLFEGWKPELFLGIELNGKTLGIIGLGRIGKAVA
KRAQAFGMKVIYHNRRPLTSEEEKNLDVEYRSLEQLLKESDFISLHVPLTSETYHLLSRSKLKLMKPSAVLINTSRGAVV
DEEALIEFLQQGKIAAAGLDVYENEPEVPYALKELDNVVLLPHIGSATVETRNNMAVLVAKNVLAVLEGKKPLTPVY

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=314, Percent_Identity=42.3566878980892, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI23308577, Length=312, Percent_Identity=35.2564102564103, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI61743967, Length=257, Percent_Identity=35.0194552529183, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI4557497, Length=257, Percent_Identity=35.0194552529183, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI145580578, Length=259, Percent_Identity=33.5907335907336, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI4557499, Length=259, Percent_Identity=33.5907335907336, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI145580575, Length=293, Percent_Identity=31.740614334471, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI87082289, Length=261, Percent_Identity=44.0613026819923, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1787645, Length=258, Percent_Identity=36.4341085271318, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789279, Length=293, Percent_Identity=33.1058020477816, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788660, Length=255, Percent_Identity=27.0588235294118, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=311, Percent_Identity=32.1543408360129, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25147481, Length=290, Percent_Identity=28.6206896551724, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324055, Length=318, Percent_Identity=37.4213836477987, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6320925, Length=311, Percent_Identity=29.903536977492, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322116, Length=299, Percent_Identity=31.1036789297659, Blast_Score=145, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6324964, Length=245, Percent_Identity=27.3469387755102, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321253, Length=172, Percent_Identity=31.3953488372093, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325144, Length=172, Percent_Identity=31.9767441860465, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324980, Length=176, Percent_Identity=28.4090909090909, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571528, Length=314, Percent_Identity=40.7643312101911, Blast_Score=214, Evalue=7e-56,
Organism=Drosophila melanogaster, GI45552429, Length=320, Percent_Identity=35.9375, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24585514, Length=320, Percent_Identity=35.9375, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI28574282, Length=320, Percent_Identity=35.9375, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI45551003, Length=320, Percent_Identity=35.9375, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI28574284, Length=320, Percent_Identity=35.9375, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI28574286, Length=289, Percent_Identity=38.0622837370242, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI19921140, Length=317, Percent_Identity=33.4384858044164, Blast_Score=171, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24585516, Length=320, Percent_Identity=31.25, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI62472511, Length=225, Percent_Identity=41.3333333333333, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24646446, Length=225, Percent_Identity=41.3333333333333, Blast_Score=150, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24646448, Length=225, Percent_Identity=41.3333333333333, Blast_Score=150, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24646452, Length=225, Percent_Identity=41.3333333333333, Blast_Score=150, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24646450, Length=225, Percent_Identity=41.3333333333333, Blast_Score=150, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 35013; Mature: 35013

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIFVTYKIPEIGLKLLSKFNVEVNEEDRTLSKKEIIERAQDATALVTLLSDNIDAEII
CCEEEEEEECHHHHHHHHHHCCCEECCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
NALPRLKIIANYAVGFNNIDIEAAKAKGVIVTNTPDILTDASADLAMALLLATARRIVEA
HHCCHHEEEEEEEECCCCCEEEEECCCCEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHH
DKFVRKGLFEGWKPELFLGIELNGKTLGIIGLGRIGKAVAKRAQAFGMKVIYHNRRPLTS
HHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCC
EEEKNLDVEYRSLEQLLKESDFISLHVPLTSETYHLLSRSKLKLMKPSAVLINTSRGAVV
CHHCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCEEECCCEEEEECCCCCEE
DEEALIEFLQQGKIAAAGLDVYENEPEVPYALKELDNVVLLPHIGSATVETRNNMAVLVA
CHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHCCCEEEEECCCCCEEECCCCEEEEEE
KNVLAVLEGKKPLTPVY
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEKIFVTYKIPEIGLKLLSKFNVEVNEEDRTLSKKEIIERAQDATALVTLLSDNIDAEII
CCEEEEEEECHHHHHHHHHHCCCEECCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
NALPRLKIIANYAVGFNNIDIEAAKAKGVIVTNTPDILTDASADLAMALLLATARRIVEA
HHCCHHEEEEEEEECCCCCEEEEECCCCEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHH
DKFVRKGLFEGWKPELFLGIELNGKTLGIIGLGRIGKAVAKRAQAFGMKVIYHNRRPLTS
HHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCC
EEEKNLDVEYRSLEQLLKESDFISLHVPLTSETYHLLSRSKLKLMKPSAVLINTSRGAVV
CHHCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCEEECCCEEEEECCCCCEE
DEEALIEFLQQGKIAAAGLDVYENEPEVPYALKELDNVVLLPHIGSATVETRNNMAVLVA
CHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHCCCEEEEECCCCCEEECCCCEEEEEE
KNVLAVLEGKKPLTPVY
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA