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The map label for this gene is pdxT

Identifier: 239617196

GI number: 239617196

Start: 854898

End: 855476

Strand: Reverse

Name: pdxT

Synonym: Kole_0803

Alternate gene names: 239617196

Gene position: 855476-854898 (Counterclockwise)

Preceding gene: 239617197

Following gene: 239617195

Centisome position: 37.16

GC content: 43.87

Gene sequence:

>579_bases
TTGAAAATAGGGGTTCTCGGTATCCAGGGAGACATCCAGGAGCACCTTAGAATGATAGAAAAAACGGGAAATGAACCCCT
ATGGGTAAAAAGCACTTCGGAGCTTGCGGAAGTTTCCGGGTTGATCATGCCTGGCGGCGAATCGACAACCATGATACGGT
TGATGAAAAAATATGAACTCTGGGACGCTCTGAGAGAAGCGATTGCTTCAGGGCTACCTGTTTACGCTACCTGTGCAGGC
ATGATACTTTTAGCGCGTGAAATAATAAATTATCCCGAACAGGAAACTCTCGGTGTTCTGGACATAGCGGTTGAAAGAAA
CGGTTATGGAAGACAGGTAGCGAGTTTTGAAACCGATCTCGAAATACCGGCTATAGGGGATACCCCTTTTAGGGCGATTT
TTATTAGAGCTCCTATAATAGAAAAATGCGGGGACAGTGTTGAAGTACTCTCCACGTATAAAGAAAAACCGGTGTTAGTT
AAGCAGGGCAAAATTCTCGCATCATCCTTTCATCCCGAGCTAACGGATGATTTGAGAATTCACGAATACTTCGTGAAAGA
AATAATTGGGAAAGAATAA

Upstream 100 bases:

>100_bases
CCAAAATGCTTGCAAAGATTAGTGAAGACATTGGGCAGCCGATGGAAGGTAAAGATATTGAAGAGCTTGATGTTAAACTA
CAGGAGAGGGGCTGGTAAGT

Downstream 100 bases:

>100_bases
GGATCGAGAACCGGGAGTCCGAGATCCGAGAAAACAGGACGCTTGCGAGGTTGTGCGCTGCGGAACAGCGGACACGAATC
CCTCCGGGATTGTCACGAAC

Product: SNO glutamine amidotransferase

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MKIGVLGIQGDIQEHLRMIEKTGNEPLWVKSTSELAEVSGLIMPGGESTTMIRLMKKYELWDALREAIASGLPVYATCAG
MILLAREIINYPEQETLGVLDIAVERNGYGRQVASFETDLEIPAIGDTPFRAIFIRAPIIEKCGDSVEVLSTYKEKPVLV
KQGKILASSFHPELTDDLRIHEYFVKEIIGKE

Sequences:

>Translated_192_residues
MKIGVLGIQGDIQEHLRMIEKTGNEPLWVKSTSELAEVSGLIMPGGESTTMIRLMKKYELWDALREAIASGLPVYATCAG
MILLAREIINYPEQETLGVLDIAVERNGYGRQVASFETDLEIPAIGDTPFRAIFIRAPIIEKCGDSVEVLSTYKEKPVLV
KQGKILASSFHPELTDDLRIHEYFVKEIIGKE
>Mature_192_residues
MKIGVLGIQGDIQEHLRMIEKTGNEPLWVKSTSELAEVSGLIMPGGESTTMIRLMKKYELWDALREAIASGLPVYATCAG
MILLAREIINYPEQETLGVLDIAVERNGYGRQVASFETDLEIPAIGDTPFRAIFIRAPIIEKCGDSVEVLSTYKEKPVLV
KQGKILASSFHPELTDDLRIHEYFVKEIIGKE

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=210, Percent_Identity=36.1904761904762, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323995, Length=217, Percent_Identity=36.8663594470046, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321048, Length=217, Percent_Identity=36.405529953917, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_KOSOT (C5CG73)

Other databases:

- EMBL:   CP001634
- RefSeq:   YP_002940518.1
- ProteinModelPortal:   C5CG73
- GeneID:   7968155
- GenomeReviews:   CP001634_GR
- KEGG:   kol:Kole_0803
- HAMAP:   MF_01615
- InterPro:   IPR002161
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 21378; Mature: 21378

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 78-78 ACT_SITE 171-171 ACT_SITE 173-173 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGVLGIQGDIQEHLRMIEKTGNEPLWVKSTSELAEVSGLIMPGGESTTMIRLMKKYEL
CEEEEEECCCCHHHHHHHHHHCCCCCEEEECHHHHHHHCCEEECCCCCHHHHHHHHHHHH
WDALREAIASGLPVYATCAGMILLAREIINYPEQETLGVLDIAVERNGYGRQVASFETDL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHCEEEEEEEECCCCCCCHHCCCCCC
EIPAIGDTPFRAIFIRAPIIEKCGDSVEVLSTYKEKPVLVKQGKILASSFHPELTDDLRI
CCCCCCCCCCEEEEEECCHHHHCCCHHHHHHHHCCCCEEEECCCEEECCCCCCCCCHHHH
HEYFVKEIIGKE
HHHHHHHHHCCC
>Mature Secondary Structure
MKIGVLGIQGDIQEHLRMIEKTGNEPLWVKSTSELAEVSGLIMPGGESTTMIRLMKKYEL
CEEEEEECCCCHHHHHHHHHHCCCCCEEEECHHHHHHHCCEEECCCCCHHHHHHHHHHHH
WDALREAIASGLPVYATCAGMILLAREIINYPEQETLGVLDIAVERNGYGRQVASFETDL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHCEEEEEEEECCCCCCCHHCCCCCC
EIPAIGDTPFRAIFIRAPIIEKCGDSVEVLSTYKEKPVLVKQGKILASSFHPELTDDLRI
CCCCCCCCCCEEEEEECCHHHHCCCHHHHHHHHCCCCEEEECCCEEECCCCCCCCCHHHH
HEYFVKEIIGKE
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA