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The map label for this gene is ydeM [H]

Identifier: 239617163

GI number: 239617163

Start: 822228

End: 823562

Strand: Reverse

Name: ydeM [H]

Synonym: Kole_0769

Alternate gene names: 239617163

Gene position: 823562-822228 (Counterclockwise)

Preceding gene: 239617164

Following gene: 239617162

Centisome position: 35.77

GC content: 34.91

Gene sequence:

>1335_bases
ATGCATGAAAGTCATTACAACTTAGTAATGAAAGTAGACACAGACGCTTTTTTGCTTTTCAATTCGTTATCCGGTTCAGC
TGACTTAGTAGATTCAGCGGTTGTAAAAGTCCTTAGAGGGCAACTAATTAACGATAGAGTCGAAAAATACTTAATATCAA
GAGGTTATCTTACCAGCAAGACAATAATTGAAGAGAAAAGTGATATGAAACGTTTCTTCACAGAAGTTTGCCAAACTTTA
TTTAAGAACAAAGAATATGGTTTTGTAATTACATATAAATGCAATTTAGCTTGTCCCTACTGTTTTGAGGCTTCTGTGAA
AGAGGGTATTGTGGGTAAAAGAACTACTGTCATAGATGATCGGAGAATAGAAAAAGCTTTCGAGGTCATTGACTTTTTTG
ATGATCTTGGAGATAATAAAAAGATTTCTATTAAGTTGTATGGCGGAGAACCTTTAATGCCAGAAAATAGAAGAGCTGTT
GAATATATAATAAAAAACGCAATAACAAGAGATAGAAAAATTGCAGTTATTACAAATGGTATCTATCTTAACGAGTATAT
AGATTATCTGGCAAAATACAAAGACAGAATAGATTTTGTCCAGGTGAGTTTGGATGGTCCAAAAAATGAACATGATAAAA
GACGGGTCAGTATTTCCGGAAATGGAACTTTCGATAGAATTATTCATAATATCGAGTTAGTGTTACTCAAAGGCATAAAA
ACATCGATCCGAATCCTTGTGGATTCAGAAAACGAACATACTATTCCAGAACTGATAAAATTCCTAAAATACCATAATTG
GCCGAATCAAGACAAATTATCTTGTTTTTTTGCATCCACGTCGGCCCCTAATAGCAACACGTTACATAACTATTCGAAGG
TAGCAATTTGGAAGAAGATCTGTCAGTATTATGAAAAAGGTTTATTAAACGAAATATTTAGCGAACCAATAGTCTATCCT
TTTAAGGATTTGTTCATAAACAAAGTATGGAGTCCTCGTTTCTTCGCATGTAATGCGCATACGGGGCTTATGATTTTTGA
TCCTTACGGAATGATTTATCCCTGCTGGAAAGCTGTGGGGCAATCTGAACTTTCCATCGGAAGATTTTATCCCTCATTTA
AACTAAATAAAGCAAGCAAAAGGTGGAAATCGAGATCTGTTTTTATGTTGAAGAAATGTGCTGGCTGTGAATTCTCAGCG
ATTTGTGGTGGAGGTTGTGCCTATTCTGCATACGTAAAGCATGGCACAATTCTAGAAGGCTCATGCGAAGAAATGGAGGT
TATTAAGAATGTTTACTTGCCATTTGCCTACCAAAGATATCTTAAAAAATCGTGA

Upstream 100 bases:

>100_bases
ATACTATCGAGTAGTGTACTTTGAAAATTGTTAATTTTTAATGCCCATATGATGCTTTTCTGCCCCTAACGTAGATTAGC
AAAAGTGAGGTGTAAGGGTG

Downstream 100 bases:

>100_bases
CTGTATGATTTGCATTAAAGATAACTAATATGAGTCTCAACTTATGCACTTCGAGTTAACAAGACGAATAAAGGAAAAAC
TGAACTCTGGTTCTATTATA

Product: Radical SAM domain protein

Products: NA

Alternate protein names: AnSME homolog [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MHESHYNLVMKVDTDAFLLFNSLSGSADLVDSAVVKVLRGQLINDRVEKYLISRGYLTSKTIIEEKSDMKRFFTEVCQTL
FKNKEYGFVITYKCNLACPYCFEASVKEGIVGKRTTVIDDRRIEKAFEVIDFFDDLGDNKKISIKLYGGEPLMPENRRAV
EYIIKNAITRDRKIAVITNGIYLNEYIDYLAKYKDRIDFVQVSLDGPKNEHDKRRVSISGNGTFDRIIHNIELVLLKGIK
TSIRILVDSENEHTIPELIKFLKYHNWPNQDKLSCFFASTSAPNSNTLHNYSKVAIWKKICQYYEKGLLNEIFSEPIVYP
FKDLFINKVWSPRFFACNAHTGLMIFDPYGMIYPCWKAVGQSELSIGRFYPSFKLNKASKRWKSRSVFMLKKCAGCEFSA
ICGGGCAYSAYVKHGTILEGSCEEMEVIKNVYLPFAYQRYLKKS

Sequences:

>Translated_444_residues
MHESHYNLVMKVDTDAFLLFNSLSGSADLVDSAVVKVLRGQLINDRVEKYLISRGYLTSKTIIEEKSDMKRFFTEVCQTL
FKNKEYGFVITYKCNLACPYCFEASVKEGIVGKRTTVIDDRRIEKAFEVIDFFDDLGDNKKISIKLYGGEPLMPENRRAV
EYIIKNAITRDRKIAVITNGIYLNEYIDYLAKYKDRIDFVQVSLDGPKNEHDKRRVSISGNGTFDRIIHNIELVLLKGIK
TSIRILVDSENEHTIPELIKFLKYHNWPNQDKLSCFFASTSAPNSNTLHNYSKVAIWKKICQYYEKGLLNEIFSEPIVYP
FKDLFINKVWSPRFFACNAHTGLMIFDPYGMIYPCWKAVGQSELSIGRFYPSFKLNKASKRWKSRSVFMLKKCAGCEFSA
ICGGGCAYSAYVKHGTILEGSCEEMEVIKNVYLPFAYQRYLKKS
>Mature_444_residues
MHESHYNLVMKVDTDAFLLFNSLSGSADLVDSAVVKVLRGQLINDRVEKYLISRGYLTSKTIIEEKSDMKRFFTEVCQTL
FKNKEYGFVITYKCNLACPYCFEASVKEGIVGKRTTVIDDRRIEKAFEVIDFFDDLGDNKKISIKLYGGEPLMPENRRAV
EYIIKNAITRDRKIAVITNGIYLNEYIDYLAKYKDRIDFVQVSLDGPKNEHDKRRVSISGNGTFDRIIHNIELVLLKGIK
TSIRILVDSENEHTIPELIKFLKYHNWPNQDKLSCFFASTSAPNSNTLHNYSKVAIWKKICQYYEKGLLNEIFSEPIVYP
FKDLFINKVWSPRFFACNAHTGLMIFDPYGMIYPCWKAVGQSELSIGRFYPSFKLNKASKRWKSRSVFMLKKCAGCEFSA
ICGGGCAYSAYVKHGTILEGSCEEMEVIKNVYLPFAYQRYLKKS

Specific function: Unknown

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081923, Length=363, Percent_Identity=24.7933884297521, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 51174; Mature: 51174

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHESHYNLVMKVDTDAFLLFNSLSGSADLVDSAVVKVLRGQLINDRVEKYLISRGYLTSK
CCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
TIIEEKSDMKRFFTEVCQTLFKNKEYGFVITYKCNLACPYCFEASVKEGIVGKRTTVIDD
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCHHHCCCCCCCEEEECH
RRIEKAFEVIDFFDDLGDNKKISIKLYGGEPLMPENRRAVEYIIKNAITRDRKIAVITNG
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECC
IYLNEYIDYLAKYKDRIDFVQVSLDGPKNEHDKRRVSISGNGTFDRIIHNIELVLLKGIK
EEHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHEEEECCC
TSIRILVDSENEHTIPELIKFLKYHNWPNQDKLSCFFASTSAPNSNTLHNYSKVAIWKKI
EEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHH
CQYYEKGLLNEIFSEPIVYPFKDLFINKVWSPRFFACNAHTGLMIFDPYGMIYPCWKAVG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHHHCC
QSELSIGRFYPSFKLNKASKRWKSRSVFMLKKCAGCEFSAICGGGCAYSAYVKHGTILEG
CCCCCHHHCCCCCEECHHHHHHHCCCCEEEHHHCCCCEEEECCCCCEEEHHHCCCCEECC
SCEEMEVIKNVYLPFAYQRYLKKS
CHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MHESHYNLVMKVDTDAFLLFNSLSGSADLVDSAVVKVLRGQLINDRVEKYLISRGYLTSK
CCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
TIIEEKSDMKRFFTEVCQTLFKNKEYGFVITYKCNLACPYCFEASVKEGIVGKRTTVIDD
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCHHHCCCCCCCEEEECH
RRIEKAFEVIDFFDDLGDNKKISIKLYGGEPLMPENRRAVEYIIKNAITRDRKIAVITNG
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECC
IYLNEYIDYLAKYKDRIDFVQVSLDGPKNEHDKRRVSISGNGTFDRIIHNIELVLLKGIK
EEHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHEEEECCC
TSIRILVDSENEHTIPELIKFLKYHNWPNQDKLSCFFASTSAPNSNTLHNYSKVAIWKKI
EEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHH
CQYYEKGLLNEIFSEPIVYPFKDLFINKVWSPRFFACNAHTGLMIFDPYGMIYPCWKAVG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCHHHHHHHHCC
QSELSIGRFYPSFKLNKASKRWKSRSVFMLKKCAGCEFSAICGGGCAYSAYVKHGTILEG
CCCCCHHHCCCCCEECHHHHHHHCCCCEEEHHHCCCCEEEECCCCCEEEHHHCCCCEECC
SCEEMEVIKNVYLPFAYQRYLKKS
CHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]