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The map label for this gene is iadA [H]

Identifier: 239617044

GI number: 239617044

Start: 687670

End: 688809

Strand: Reverse

Name: iadA [H]

Synonym: Kole_0645

Alternate gene names: 239617044

Gene position: 688809-687670 (Counterclockwise)

Preceding gene: 239617057

Following gene: 239617040

Centisome position: 29.92

GC content: 44.47

Gene sequence:

>1140_bases
ATGTTGTTACTCAAGGATTTGCCAGTGTATTCTTCTCAATATATAGGAATCAAAGATATTTTGATATGTGGGACCAGAGT
AGAAGCGATAGCAGACAAAATTTCAATTGACGGCATCGACAAACTTAAGACACTTTATTTTGATTCAAAGAGGCATTTTG
CTGTGCCTGGTTTTGTGGATGGTCATGTTCATCTCATTGGTGGAGGTGGAGAAGGAGGGTTCAGCACCCGGACACGAGAG
GGAACTCCTGAGGAATTTGCCTGCTGTGGCACTACGGCAGTTGTTGGAATGTTAGGTACTGATAGCATAACCAGAGATCA
CATTTCGCTTCTTGCCAAAGCGAGGGCTTTCAGAGAATGGGGACTTGGTGCATGGGTGCTCACAGGTTCATATAGATACC
CAATCAAGACCATTACTGGTGACCTTGCTAAAGACATCGTACTAATACCCGAAGTCATAGGTGTGGGTGAAGTAGCGGTT
TCTGATCACAGGGGAAGCAATATCTCTGGAGCGGAACTCCAGCGCTTGGCGATGGATACGAGAGTGGCCGCTATGCTTGC
TGGTAAAAAAGGAATAGTCGTTTGCCATATGGGAAACGCGCCCGAAAGGATGAAACCACTGTTCAAGGCGGTCGAAAACG
GTTCACTACCTCCAAAACACTTCATACCAACTCACATTTCAAGAAACGATAAACTCCTTGAAGAAGGAATCAAATGGGTC
AAGGAAAATGACGGATTCATAGATCTGACAGCTGGAAAAAATACGTCAAGCATTATCATGAATTTAAGGGCAGAGCTTGG
AAATCTCAATCGGATAAGTGTTTCTTCCGATGGTCTCGGAAGCCTTCCCAAATTTGACGAAGATGGCAAACTTATTGAAA
TCACCGCAGCACCGGTTGATGGACTTTTAAGAGTATTCAAAGAGCTGCTGCAAAAGGGTCTCAGCGTTGAAGAAGCTATC
AAACCCGTGTGTTCCGTTCCAGCTACTGCGCTTGGGTTGAACCGTTTTGGTTATGGAAAGATTGAGAAAGGTTATTCGGC
TTCTTTGCTTGTTTTTAACACTGAAAATCTAAACCTGGAGCTCGTGTTAGCCAACGGTAAGATAGTAGTGCAGAAAGGCA
CTCTTGCCGATCAAAGATGA

Upstream 100 bases:

>100_bases
GATAACCCTTATTTGGATTCACTTTCATACCATTCAACCGATGGACAAATGATAAACTTGAGTAAATTGGGAATTTTCAA
AACTTGGGAGAGTGATGGAA

Downstream 100 bases:

>100_bases
AGATGAAAGTGGATAACTTGAGCGATAGTGTATCTATAGGGAAAACTTAAACTCGAAAAGAAAGATACTTCAGGATTCAG
CCTAGAGGATGTCAGTCACT

Product: isoaspartyl dipeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MLLLKDLPVYSSQYIGIKDILICGTRVEAIADKISIDGIDKLKTLYFDSKRHFAVPGFVDGHVHLIGGGGEGGFSTRTRE
GTPEEFACCGTTAVVGMLGTDSITRDHISLLAKARAFREWGLGAWVLTGSYRYPIKTITGDLAKDIVLIPEVIGVGEVAV
SDHRGSNISGAELQRLAMDTRVAAMLAGKKGIVVCHMGNAPERMKPLFKAVENGSLPPKHFIPTHISRNDKLLEEGIKWV
KENDGFIDLTAGKNTSSIIMNLRAELGNLNRISVSSDGLGSLPKFDEDGKLIEITAAPVDGLLRVFKELLQKGLSVEEAI
KPVCSVPATALGLNRFGYGKIEKGYSASLLVFNTENLNLELVLANGKIVVQKGTLADQR

Sequences:

>Translated_379_residues
MLLLKDLPVYSSQYIGIKDILICGTRVEAIADKISIDGIDKLKTLYFDSKRHFAVPGFVDGHVHLIGGGGEGGFSTRTRE
GTPEEFACCGTTAVVGMLGTDSITRDHISLLAKARAFREWGLGAWVLTGSYRYPIKTITGDLAKDIVLIPEVIGVGEVAV
SDHRGSNISGAELQRLAMDTRVAAMLAGKKGIVVCHMGNAPERMKPLFKAVENGSLPPKHFIPTHISRNDKLLEEGIKWV
KENDGFIDLTAGKNTSSIIMNLRAELGNLNRISVSSDGLGSLPKFDEDGKLIEITAAPVDGLLRVFKELLQKGLSVEEAI
KPVCSVPATALGLNRFGYGKIEKGYSASLLVFNTENLNLELVLANGKIVVQKGTLADQR
>Mature_379_residues
MLLLKDLPVYSSQYIGIKDILICGTRVEAIADKISIDGIDKLKTLYFDSKRHFAVPGFVDGHVHLIGGGGEGGFSTRTRE
GTPEEFACCGTTAVVGMLGTDSITRDHISLLAKARAFREWGLGAWVLTGSYRYPIKTITGDLAKDIVLIPEVIGVGEVAV
SDHRGSNISGAELQRLAMDTRVAAMLAGKKGIVVCHMGNAPERMKPLFKAVENGSLPPKHFIPTHISRNDKLLEEGIKWV
KENDGFIDLTAGKNTSSIIMNLRAELGNLNRISVSSDGLGSLPKFDEDGKLIEITAAPVDGLLRVFKELLQKGLSVEEAI
KPVCSVPATALGLNRFGYGKIEKGYSASLLVFNTENLNLELVLANGKIVVQKGTLADQR

Specific function: Catalyzes the hydrolytic cleavage of a subset of L- isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation. The best substrate for the enzyme reported thus far is iso-Asp-Leu [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M38 family [H]

Homologues:

Organism=Escherichia coli, GI1790784, Length=377, Percent_Identity=38.7267904509284, Blast_Score=240, Evalue=9e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059
- InterPro:   IPR010229 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.4.19.- [C]

Molecular weight: Translated: 40868; Mature: 40868

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLKDLPVYSSQYIGIKDILICGTRVEAIADKISIDGIDKLKTLYFDSKRHFAVPGFVD
CEEEECCCCCCCCCCCHHHEEEECCHHHHHHHHHCCCCHHHHHHEEECCCCCEECCCEEC
GHVHLIGGGGEGGFSTRTREGTPEEFACCGTTAVVGMLGTDSITRDHISLLAKARAFREW
CEEEEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHC
GLGAWVLTGSYRYPIKTITGDLAKDIVLIPEVIGVGEVAVSDHRGSNISGAELQRLAMDT
CCEEEEEECCCCCCHHHHHHHHHHCEEEEHHHHCCCCEEEECCCCCCCCHHHHHHHHHHH
RVAAMLAGKKGIVVCHMGNAPERMKPLFKAVENGSLPPKHFIPTHISRNDKLLEEGIKWV
HHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KENDGFIDLTAGKNTSSIIMNLRAELGNLNRISVSSDGLGSLPKFDEDGKLIEITAAPVD
CCCCCEEEEECCCCHHHHHHEEHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCHH
GLLRVFKELLQKGLSVEEAIKPVCSVPATALGLNRFGYGKIEKGYSASLLVFNTENLNLE
HHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEE
LVLANGKIVVQKGTLADQR
EEEECCEEEEEECCCCCCC
>Mature Secondary Structure
MLLLKDLPVYSSQYIGIKDILICGTRVEAIADKISIDGIDKLKTLYFDSKRHFAVPGFVD
CEEEECCCCCCCCCCCHHHEEEECCHHHHHHHHHCCCCHHHHHHEEECCCCCEECCCEEC
GHVHLIGGGGEGGFSTRTREGTPEEFACCGTTAVVGMLGTDSITRDHISLLAKARAFREW
CEEEEEECCCCCCCCCCCCCCCCHHHHHCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHC
GLGAWVLTGSYRYPIKTITGDLAKDIVLIPEVIGVGEVAVSDHRGSNISGAELQRLAMDT
CCEEEEEECCCCCCHHHHHHHHHHCEEEEHHHHCCCCEEEECCCCCCCCHHHHHHHHHHH
RVAAMLAGKKGIVVCHMGNAPERMKPLFKAVENGSLPPKHFIPTHISRNDKLLEEGIKWV
HHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KENDGFIDLTAGKNTSSIIMNLRAELGNLNRISVSSDGLGSLPKFDEDGKLIEITAAPVD
CCCCCEEEEECCCCHHHHHHEEHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCHH
GLLRVFKELLQKGLSVEEAIKPVCSVPATALGLNRFGYGKIEKGYSASLLVFNTENLNLE
HHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEE
LVLANGKIVVQKGTLADQR
EEEECCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7876157; 7610040; 9278503 [H]