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The map label for this gene is dapL [H]

Identifier: 239616934

GI number: 239616934

Start: 583716

End: 584858

Strand: Reverse

Name: dapL [H]

Synonym: Kole_0532

Alternate gene names: 239616934

Gene position: 584858-583716 (Counterclockwise)

Preceding gene: 239616940

Following gene: 239616933

Centisome position: 25.41

GC content: 42.43

Gene sequence:

>1143_bases
ATGATCGAAATTGGCAAGGTGCACGAATTAAGGCACAGATTGCATAAAAATCCGGAAGGTGGTTTTAGGGAATATGAAAC
AAAAAAGATCTTACTGAAATTCCTTTCAGATTTACCGAAAAATTACTTCAAAATTTATGAAGTTCTGGAAACAGGATTGG
TGGTAGAATATCGTAATGCAGAAAATAAGAGCTTTGTCCTTTTCAGAGCAGATATGGATGCCCTGCCCATTGAAGAAGAA
ACTAATGTGGATTTCGCATCGGAAAACCCTGGCTGGATGCACGCATGCGGTCACGATATCCATATGTCCGTGCTTACTGG
ATTGATCAAACAAGTAGCAAGAGAACTTTCAGAAGCAAATATTTTGTTCTTCTTTCAGCCAGCCGAAGAAGGCCCCGGGG
GAGCGAAACCTTTCCTTGAAAGCGGATTTCTTGACTCATATGATGTAAAAGCTGCTTTCGCATTACACGTAAGCCCTGAT
TATAAAATGGGTACGGTTGCTTCTAAAGAAGGAGTTATTTTCGCGAGCCCAACGGAATTCGACGTTATCTTTAAAGGTGA
AAGTGCTCATGGAGCCAAACCTGATGAAGGCAGGGATACAATACTACCCGCATCAGAATTCATAACCGCTTTTTATTCCG
CTCTCACAAGGTTCATCCCGGAAGAGGAGCGTTATGTGTTCACCATCGGAAAATTGGTAGCGGGTGATCGAAGGAACATA
GTGGCGGAAAAATCAATAATTGAGGGAACCTATAGGGTTCTTGACATAAAAACCAAAGAGAAAATAGACGAGCTCATGGA
AAGATTTACTGAAAACATAAGCAAAACATGGAAGGTTCAGGGCGAGGTTCGTTATGGAGCGTATTATCCAGTACTTATGA
ACGACTCCAGGCTCTTTAAAGCCCTTGCGAAAACGGTCAAAAGTATTGGTTTGGAATTTGCTGAATGCGAGACAAAGTTC
ACCGGCGAGGATTTCGCGTTTTTCAGCCAGCGGTACCCTTCACTGATGTTCTGGCTTGGATGTGCTACCGAAACGATGAA
AGCCGGATTACACACATCAAAGTTTTTACCGGAGGACAGATGCATTGATTTTGGCGTGAAGGTATTTTATTCTCTACTCA
AGGAAGTGAGTAAGGAGGATTGA

Upstream 100 bases:

>100_bases
AGGGTCACCCCCACCATTTTCACACAGCAAAACTGTGCTATATTTGTTTTGAATCAACGTGTTGTAATAATTATACTCCA
AAAAATAGGGGGATATTTAA

Downstream 100 bases:

>100_bases
ATTGGAATTTGTTGTCATAGATACCGAAACCACCGGTTCCAGCCCTCATAAAGGGGCAGAGTTGATAGAAATCGCGGGCG
TGGTTGTCAGGGACTGGGAA

Product: amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MIEIGKVHELRHRLHKNPEGGFREYETKKILLKFLSDLPKNYFKIYEVLETGLVVEYRNAENKSFVLFRADMDALPIEEE
TNVDFASENPGWMHACGHDIHMSVLTGLIKQVARELSEANILFFFQPAEEGPGGAKPFLESGFLDSYDVKAAFALHVSPD
YKMGTVASKEGVIFASPTEFDVIFKGESAHGAKPDEGRDTILPASEFITAFYSALTRFIPEEERYVFTIGKLVAGDRRNI
VAEKSIIEGTYRVLDIKTKEKIDELMERFTENISKTWKVQGEVRYGAYYPVLMNDSRLFKALAKTVKSIGLEFAECETKF
TGEDFAFFSQRYPSLMFWLGCATETMKAGLHTSKFLPEDRCIDFGVKVFYSLLKEVSKED

Sequences:

>Translated_380_residues
MIEIGKVHELRHRLHKNPEGGFREYETKKILLKFLSDLPKNYFKIYEVLETGLVVEYRNAENKSFVLFRADMDALPIEEE
TNVDFASENPGWMHACGHDIHMSVLTGLIKQVARELSEANILFFFQPAEEGPGGAKPFLESGFLDSYDVKAAFALHVSPD
YKMGTVASKEGVIFASPTEFDVIFKGESAHGAKPDEGRDTILPASEFITAFYSALTRFIPEEERYVFTIGKLVAGDRRNI
VAEKSIIEGTYRVLDIKTKEKIDELMERFTENISKTWKVQGEVRYGAYYPVLMNDSRLFKALAKTVKSIGLEFAECETKF
TGEDFAFFSQRYPSLMFWLGCATETMKAGLHTSKFLPEDRCIDFGVKVFYSLLKEVSKED
>Mature_380_residues
MIEIGKVHELRHRLHKNPEGGFREYETKKILLKFLSDLPKNYFKIYEVLETGLVVEYRNAENKSFVLFRADMDALPIEEE
TNVDFASENPGWMHACGHDIHMSVLTGLIKQVARELSEANILFFFQPAEEGPGGAKPFLESGFLDSYDVKAAFALHVSPD
YKMGTVASKEGVIFASPTEFDVIFKGESAHGAKPDEGRDTILPASEFITAFYSALTRFIPEEERYVFTIGKLVAGDRRNI
VAEKSIIEGTYRVLDIKTKEKIDELMERFTENISKTWKVQGEVRYGAYYPVLMNDSRLFKALAKTVKSIGLEFAECETKF
TGEDFAFFSQRYPSLMFWLGCATETMKAGLHTSKFLPEDRCIDFGVKVFYSLLKEVSKED

Specific function: Catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapL subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=220, Percent_Identity=33.1818181818182, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.47 [H]

Molecular weight: Translated: 43225; Mature: 43225

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIGKVHELRHRLHKNPEGGFREYETKKILLKFLSDLPKNYFKIYEVLETGLVVEYRNA
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
ENKSFVLFRADMDALPIEEETNVDFASENPGWMHACGHDIHMSVLTGLIKQVARELSEAN
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCC
ILFFFQPAEEGPGGAKPFLESGFLDSYDVKAAFALHVSPDYKMGTVASKEGVIFASPTEF
EEEEEECCCCCCCCCCCHHHCCCCCCCCCCEEEEEEECCCCCCCCEECCCCEEEECCCCE
DVIFKGESAHGAKPDEGRDTILPASEFITAFYSALTRFIPEEERYVFTIGKLVAGDRRNI
EEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCH
VAEKSIIEGTYRVLDIKTKEKIDELMERFTENISKTWKVQGEVRYGAYYPVLMNDSRLFK
HHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHEEEEEEEEEECEEEEEEECCHHHHH
ALAKTVKSIGLEFAECETKFTGEDFAFFSQRYPSLMFWLGCATETMKAGLHTSKFLPEDR
HHHHHHHHHCCCHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCHH
CIDFGVKVFYSLLKEVSKED
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIEIGKVHELRHRLHKNPEGGFREYETKKILLKFLSDLPKNYFKIYEVLETGLVVEYRNA
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
ENKSFVLFRADMDALPIEEETNVDFASENPGWMHACGHDIHMSVLTGLIKQVARELSEAN
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCC
ILFFFQPAEEGPGGAKPFLESGFLDSYDVKAAFALHVSPDYKMGTVASKEGVIFASPTEF
EEEEEECCCCCCCCCCCHHHCCCCCCCCCCEEEEEEECCCCCCCCEECCCCEEEECCCCE
DVIFKGESAHGAKPDEGRDTILPASEFITAFYSALTRFIPEEERYVFTIGKLVAGDRRNI
EEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCH
VAEKSIIEGTYRVLDIKTKEKIDELMERFTENISKTWKVQGEVRYGAYYPVLMNDSRLFK
HHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHEEEEEEEEEECEEEEEEECCHHHHH
ALAKTVKSIGLEFAECETKFTGEDFAFFSQRYPSLMFWLGCATETMKAGLHTSKFLPEDR
HHHHHHHHHCCCHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCHH
CIDFGVKVFYSLLKEVSKED
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA