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The map label for this gene is queA [H]

Identifier: 239616653

GI number: 239616653

Start: 273647

End: 274669

Strand: Direct

Name: queA [H]

Synonym: Kole_0246

Alternate gene names: 239616653

Gene position: 273647-274669 (Clockwise)

Preceding gene: 239616652

Following gene: 239616657

Centisome position: 11.89

GC content: 42.23

Gene sequence:

>1023_bases
GTGAATATGAAAGTATCAGATTTTGATTACGAACTCCCAAAAGAGTTGATAGCTCAAGAACCTGTTCAACCTAGAGACCA
TAGTCGTTTGATGGTGATTAGAAGAGATAGGGAGAATTTTGAGCACACACATTTTTACCGGATAATCGATTATCTTGAGC
CTGGCGATTTGCTGGTTATGAATGACAGTAAGGTTATCCCCGCACGGCTGTTGGGGAAAAAATCCACTGGGGCAAAGGTG
GAGATCGTTCTGATCGAAAAAGTAGGTTCTAAACAATGGAAGGCAATCGTTAGGCCAGGAAGCAAGATAAAACGCGGTAA
TGTGCTCCTTTTTGAAGATTTGCAGTGTGAGGTTGTAGAACATTGCGATGATTCAACAAGAATCCTTGAATTCACCGGCA
GCAATCCATGGGAAAAGATAAGGCGTATCGGCAAATTGCCTATACCACCTTACATTTCTAAATACCCTGATGATCCTAAT
CTCTATCAAACGGTATATGCAAAAAAAGACGGTTCCGTTGCAGCTCCAACGGCGGGATTGCACTTCACGGATTCTTTGCT
CAAAAAACTCAAAGAAAAAGGAATCAAAATGGCTTTCGTGACACTACATGTTGGTCTTGGAACCTTCCGCCCGGTTAAGG
TCGAAAATGTCGAAGATCACGTTATGCACTCGGAATATTACACACTTCCCGAAGAAACGGTTGAGTTAATTAAGGAAACT
AAAAGAAATGGGAACCGTGTCATTGCTGTTGGAACCACGGTAGTCAGAACTCTGGAAGCCGTTGCACAAAAACACGACGG
AAAACTCGTCGCGGAATCAGGGAGAACGGATATATTCATCTATCCACCTTACGAGTTTAAAGTCATCGATGGCCTTATAA
CAAATTTTCACCTTCCAAAGTCCACGCTTTTAATGCTCGTGGCAGCTTTTACTGGACGCGAAAAAATACTAAAAGCCTAT
AATGAAGCTATAAAGCTTCGTTACCGCTTTTTCTCATTCGGCGATGCTTGCTTCATTTACTGA

Upstream 100 bases:

>100_bases
TCATGCTTGCTAGAAGCCTGAAGGCAAAAGTGATAAGCTATGATAGATTTCTTGAACATAATACTTCTGGAATCAAAATT
CTAAGACCGGAGGATCTGTT

Downstream 100 bases:

>100_bases
GTTTTCTGATTCAAGCCACGGTTCAAAAAAATGTTTCCACAGTTGTTTGAGAAGTATGGCTACCGGTGCAGCAAGAAATG
CACCCAATATTCCCAGCAAC

Product: S-adenosylmethionine/tRNA-ribosyltransferase- isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MNMKVSDFDYELPKELIAQEPVQPRDHSRLMVIRRDRENFEHTHFYRIIDYLEPGDLLVMNDSKVIPARLLGKKSTGAKV
EIVLIEKVGSKQWKAIVRPGSKIKRGNVLLFEDLQCEVVEHCDDSTRILEFTGSNPWEKIRRIGKLPIPPYISKYPDDPN
LYQTVYAKKDGSVAAPTAGLHFTDSLLKKLKEKGIKMAFVTLHVGLGTFRPVKVENVEDHVMHSEYYTLPEETVELIKET
KRNGNRVIAVGTTVVRTLEAVAQKHDGKLVAESGRTDIFIYPPYEFKVIDGLITNFHLPKSTLLMLVAAFTGREKILKAY
NEAIKLRYRFFSFGDACFIY

Sequences:

>Translated_340_residues
MNMKVSDFDYELPKELIAQEPVQPRDHSRLMVIRRDRENFEHTHFYRIIDYLEPGDLLVMNDSKVIPARLLGKKSTGAKV
EIVLIEKVGSKQWKAIVRPGSKIKRGNVLLFEDLQCEVVEHCDDSTRILEFTGSNPWEKIRRIGKLPIPPYISKYPDDPN
LYQTVYAKKDGSVAAPTAGLHFTDSLLKKLKEKGIKMAFVTLHVGLGTFRPVKVENVEDHVMHSEYYTLPEETVELIKET
KRNGNRVIAVGTTVVRTLEAVAQKHDGKLVAESGRTDIFIYPPYEFKVIDGLITNFHLPKSTLLMLVAAFTGREKILKAY
NEAIKLRYRFFSFGDACFIY
>Mature_340_residues
MNMKVSDFDYELPKELIAQEPVQPRDHSRLMVIRRDRENFEHTHFYRIIDYLEPGDLLVMNDSKVIPARLLGKKSTGAKV
EIVLIEKVGSKQWKAIVRPGSKIKRGNVLLFEDLQCEVVEHCDDSTRILEFTGSNPWEKIRRIGKLPIPPYISKYPDDPN
LYQTVYAKKDGSVAAPTAGLHFTDSLLKKLKEKGIKMAFVTLHVGLGTFRPVKVENVEDHVMHSEYYTLPEETVELIKET
KRNGNRVIAVGTTVVRTLEAVAQKHDGKLVAESGRTDIFIYPPYEFKVIDGLITNFHLPKSTLLMLVAAFTGREKILKAY
NEAIKLRYRFFSFGDACFIY

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=46.5116279069767, Blast_Score=313, Evalue=1e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38902; Mature: 38902

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKVSDFDYELPKELIAQEPVQPRDHSRLMVIRRDRENFEHTHFYRIIDYLEPGDLLVM
CCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEE
NDSKVIPARLLGKKSTGAKVEIVLIEKVGSKQWKAIVRPGSKIKRGNVLLFEDLQCEVVE
CCCCCCHHHHCCCCCCCCEEEEEEEECCCCHHHHHHCCCCCCCCCCCEEEEECCCHHHHH
HCDDSTRILEFTGSNPWEKIRRIGKLPIPPYISKYPDDPNLYQTVYAKKDGSVAAPTAGL
CCCCCCEEEEECCCCHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEEECCCCCEECCCCCC
HFTDSLLKKLKEKGIKMAFVTLHVGLGTFRPVKVENVEDHVMHSEYYTLPEETVELIKET
HHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCHHHHHHHHHEECCHHHHHHHHHH
KRNGNRVIAVGTTVVRTLEAVAQKHDGKLVAESGRTDIFIYPPYEFKVIDGLITNFHLPK
HCCCCEEEEEHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCEEEHHHHHHCCCCCH
STLLMLVAAFTGREKILKAYNEAIKLRYRFFSFGDACFIY
HHHHHHHHHHCCHHHHHHHHHHHHEEEEEEEEECCEEEEC
>Mature Secondary Structure
MNMKVSDFDYELPKELIAQEPVQPRDHSRLMVIRRDRENFEHTHFYRIIDYLEPGDLLVM
CCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEE
NDSKVIPARLLGKKSTGAKVEIVLIEKVGSKQWKAIVRPGSKIKRGNVLLFEDLQCEVVE
CCCCCCHHHHCCCCCCCCEEEEEEEECCCCHHHHHHCCCCCCCCCCCEEEEECCCHHHHH
HCDDSTRILEFTGSNPWEKIRRIGKLPIPPYISKYPDDPNLYQTVYAKKDGSVAAPTAGL
CCCCCCEEEEECCCCHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEEECCCCCEECCCCCC
HFTDSLLKKLKEKGIKMAFVTLHVGLGTFRPVKVENVEDHVMHSEYYTLPEETVELIKET
HHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECCCHHHHHHHHHEECCHHHHHHHHHH
KRNGNRVIAVGTTVVRTLEAVAQKHDGKLVAESGRTDIFIYPPYEFKVIDGLITNFHLPK
HCCCCEEEEEHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCEEEHHHHHHCCCCCH
STLLMLVAAFTGREKILKAYNEAIKLRYRFFSFGDACFIY
HHHHHHHHHHCCHHHHHHHHHHHHEEEEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA