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The map label for this gene is polC [H]

Identifier: 239616641

GI number: 239616641

Start: 262494

End: 263051

Strand: Direct

Name: polC [H]

Synonym: Kole_0234

Alternate gene names: 239616641

Gene position: 262494-263051 (Clockwise)

Preceding gene: 239616638

Following gene: 239616642

Centisome position: 11.4

GC content: 42.65

Gene sequence:

>558_bases
ATGAACAAAGTATATGTGGTCATTGATACTGAAACCACGGGACCCTCTCCTTTGAGAGGAGATCGTATTATTGAAATCGC
CGCTATTCCCATTTATAGAGGCAAGATGTTCGTTGACCTCGCCTTTCATTCACTGGTAAATCCACAGATCAGGATTCCAG
CTTATATTTCCGGGATACATGGTCTCAAGAATGAGGATGTAACAAGTGAACCCACTATAAGCGATGTTTTCCCGAGATTC
AAGGAATATCTTGGCAATGCAATTATTGTCGGTCACAGTATTGAGGTGGATATGAAGTTCTTTGACATCGCATGTAAGGA
AATAGGAACGTTTCCACTTTCTAATCAGTATATAGATACCCTTAAGATAGCAAAGGATTTGTTCAAGGAAGGGTCGTACA
GCCTTGCCGAACTTGCAAAAAGGTTGAAGATCAAGGATGCGCCAACCCATAGAGCTCTTGATGATGCGAGGGTTACTGGA
AAGGTTTTTCTGGCAATGGTGAAAAAGCTCGGTGGGTTTTCAAGCATTCGCAAATATGTGAAGCGCTGGAGGGGATAG

Upstream 100 bases:

>100_bases
CGGTGACCTGTTCTCGGGGTTTATCTATTCTCCAATCACCAACATCTATTCTCAAATTATTGCCTCTTCTCGTGATAAAA
TTCTTTCAGGAGGTATTTTG

Downstream 100 bases:

>100_bases
GTATGGTCAAGAACTTCATTCTCGATACGAACGTTCTGGTACATGATCCATATTGTATTGAAAAATTCGAGGATAACAAC
GTGATTATTCCGTTTCCTGT

Product: DNA polymerase III, epsilon subunit

Products: NA

Alternate protein names: PolIII [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MNKVYVVIDTETTGPSPLRGDRIIEIAAIPIYRGKMFVDLAFHSLVNPQIRIPAYISGIHGLKNEDVTSEPTISDVFPRF
KEYLGNAIIVGHSIEVDMKFFDIACKEIGTFPLSNQYIDTLKIAKDLFKEGSYSLAELAKRLKIKDAPTHRALDDARVTG
KVFLAMVKKLGGFSSIRKYVKRWRG

Sequences:

>Translated_185_residues
MNKVYVVIDTETTGPSPLRGDRIIEIAAIPIYRGKMFVDLAFHSLVNPQIRIPAYISGIHGLKNEDVTSEPTISDVFPRF
KEYLGNAIIVGHSIEVDMKFFDIACKEIGTFPLSNQYIDTLKIAKDLFKEGSYSLAELAKRLKIKDAPTHRALDDARVTG
KVFLAMVKKLGGFSSIRKYVKRWRG
>Mature_185_residues
MNKVYVVIDTETTGPSPLRGDRIIEIAAIPIYRGKMFVDLAFHSLVNPQIRIPAYISGIHGLKNEDVTSEPTISDVFPRF
KEYLGNAIIVGHSIEVDMKFFDIACKEIGTFPLSNQYIDTLKIAKDLFKEGSYSLAELAKRLKIKDAPTHRALDDARVTG
KVFLAMVKKLGGFSSIRKYVKRWRG

Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1786409, Length=181, Percent_Identity=29.8342541436464, Blast_Score=74, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR006054
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR023223
- InterPro:   IPR016027
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR006308
- InterPro:   IPR012337 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 20806; Mature: 20806

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVYVVIDTETTGPSPLRGDRIIEIAAIPIYRGKMFVDLAFHSLVNPQIRIPAYISGIH
CCEEEEEEECCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHCCCCEECHHHHHHHC
GLKNEDVTSEPTISDVFPRFKEYLGNAIIVGHSIEVDMKFFDIACKEIGTFPLSNQYIDT
CCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCEEECHHHHHHHHHHHCCCCCCCHHHHH
LKIAKDLFKEGSYSLAELAKRLKIKDAPTHRALDDARVTGKVFLAMVKKLGGFSSIRKYV
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KRWRG
HHHCC
>Mature Secondary Structure
MNKVYVVIDTETTGPSPLRGDRIIEIAAIPIYRGKMFVDLAFHSLVNPQIRIPAYISGIH
CCEEEEEEECCCCCCCCCCCCEEEEEEEEEEECCHHHHHHHHHHHCCCCEECHHHHHHHC
GLKNEDVTSEPTISDVFPRFKEYLGNAIIVGHSIEVDMKFFDIACKEIGTFPLSNQYIDT
CCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCEEECHHHHHHHHHHHCCCCCCCHHHHH
LKIAKDLFKEGSYSLAELAKRLKIKDAPTHRALDDARVTGKVFLAMVKKLGGFSSIRKYV
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KRWRG
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]