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The map label for this gene is nuoF2 [H]

Identifier: 239616590

GI number: 239616590

Start: 203131

End: 204930

Strand: Direct

Name: nuoF2 [H]

Synonym: Kole_0182

Alternate gene names: 239616590

Gene position: 203131-204930 (Clockwise)

Preceding gene: 239616589

Following gene: 239616591

Centisome position: 8.82

GC content: 47.56

Gene sequence:

>1800_bases
GTGCCCACAGTAGAAAACACGATCCTTATCTGTGCAGGTGGAGCCTGTATTTCGGCCGGTGAAAAAAGTGTCAAAGACGC
TCTTCAGGATTTGTTGAAAGAATATGCTCTTGATTCTGTCGTTAAGATAATTGAGACAGGTTGTATGGGCGCCTGCGACC
TTGGCCCGATAATTATTGTTTATCCCGAGGGTATTTTCTATCAGAAACTCACCCCGGAAAACGTTAGAAGGGTCGTCGAG
GAGCACCTCCTGAAAGGCCGTATTGTTGAAGACATGCTCTATAAGGGCGAATTTGGCGAGGTTAAAGCAAAACCTCAGGA
AGAGCTACCGTTCTTCACCAAGCAGGTTAAGATAGCAACGAGAAACCTCGGGGTCATCGATCCGCTCTCGATAGATGAAT
ACATAGCCCGTGATGGTTATTTTGCTCTCCACAAAGCAATCAAAGAGATGACACCGGAAGAAGTAATATCTGAGGTCAAA
AAAAGCGAGCTGCGCGGTCGCGGCGGCGCTGGATTTCCAACAGGCTTGAAATGGGAATTCGTTAGGAAGGCAAAGGGCGA
TATTAAATACGTCATCTGTAACGCAGATGAAGGAGACCCTGGAGCCTTTATGGACAGAGCTATTCTTGAAGGAGACCCAC
ACAGCGTCGTCGAAGCCATGACGATAGCCGCTTACGCGGTGGGGGCTCAAAAAGGATTCATTTACGTAAGAGCTGAGTAC
CCTCTGGCTATCGAACGATTCTCTAAAGCCCTTGAAACTGCACGCGAATATGGTTTCCTGGGAAAGAACATACTTGGAAC
CGGGTTTAGCTTTGATATAGAAATCAGAATTGGTGCCGGTGCCTTTGTTTGTGGAGAAGAAACTGCACTTATAAACTCTA
TTGAAGGTAAACGCGGTGTTCCAAGGGTAAAACCTCCATATCCAGCGACTTACGGTCTCTGGGGCAAACCCACCCTTATA
AACAACGTTGAAACTTATGCAAATATAGCCCCCATCATTTTGCACGGTGGCGACTGGTTTGCTCAGTATGGAGTCGAAGG
CTCGAGAGGAACAAAGGTCTTCGCTCTGGCAGGTAAAATAAAGAACACCGGGCTTGTTGAAGTGCCCATGGGAATTACCC
TCAGAGAACTTATATATGAAATAGGCGGCGGCGTTCCAGATGGTAAGAAACTGAAGGCGGTCCAGACTGGTGGCCCAAGC
GGTGGGTGTATCCCCGAAGAACATCTCGATACTCCCATAACCTATGACAACTTGAAGAAACTTGGGACGATTATCGGTTC
TGGCGGAATGATAGTTATGGATGAAGATGATTGTATGGTTGACGTCGCAAAATTCTTCCTTGAGTTCACCGTTGAAGAGT
CCTGTGGCCAGTGTACCCCATGCCGCGACGGTACTAAAAAGATGCTTGAGATACTGGAGAAGATCACCGCTGGTGAAGGA
ACTGAAGAAGATTTAGAGGTTCTCAAAGAACTTGGAGAACACATAATGAAGACCTCTCTATGTGGGCTCGGACAAACAGC
GCCACAGCCGGTGGTTTCAACGATGAGGTATTTCTGGGACGAATACATTGCCCATGTAAAAGAGAAGCGTTGTCCAGCCA
AGCGCTGCAAGGAACTCACCAGAGTTGTCATAGATGAAGAGAAGTGTGTGGGATGTACCGCGTGTGCCCGCGTGTGCCCG
GTGAACGGCATAATTGGGGATATAAGGAAACCCCACAGGATAGATCCAGAAATATGTACGCGATGCGGAAGCTGTATCGA
GGTTTGTAGATTCGACGCTATAAAGAAGGTTTCTCCATAA

Upstream 100 bases:

>100_bases
GGCACAGGCAAAGAGAATAATAGAACAGCACGTTATTGAAGGAAAAGTTGTAGGCGATCTCATAGTGAAGCGTGGAGAAC
TCTAAGAAGAGGTGATTTAA

Downstream 100 bases:

>100_bases
ATTCTCCTGCAAAACCCCACCGGGGTTTTGCAGTTTTTAGTTATTAATAGTGGGTGAAAGGAGGAGCATGAATGGCTGTT
GAGGAAGTGAAAGAAACGGT

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MPTVENTILICAGGACISAGEKSVKDALQDLLKEYALDSVVKIIETGCMGACDLGPIIIVYPEGIFYQKLTPENVRRVVE
EHLLKGRIVEDMLYKGEFGEVKAKPQEELPFFTKQVKIATRNLGVIDPLSIDEYIARDGYFALHKAIKEMTPEEVISEVK
KSELRGRGGAGFPTGLKWEFVRKAKGDIKYVICNADEGDPGAFMDRAILEGDPHSVVEAMTIAAYAVGAQKGFIYVRAEY
PLAIERFSKALETAREYGFLGKNILGTGFSFDIEIRIGAGAFVCGEETALINSIEGKRGVPRVKPPYPATYGLWGKPTLI
NNVETYANIAPIILHGGDWFAQYGVEGSRGTKVFALAGKIKNTGLVEVPMGITLRELIYEIGGGVPDGKKLKAVQTGGPS
GGCIPEEHLDTPITYDNLKKLGTIIGSGGMIVMDEDDCMVDVAKFFLEFTVEESCGQCTPCRDGTKKMLEILEKITAGEG
TEEDLEVLKELGEHIMKTSLCGLGQTAPQPVVSTMRYFWDEYIAHVKEKRCPAKRCKELTRVVIDEEKCVGCTACARVCP
VNGIIGDIRKPHRIDPEICTRCGSCIEVCRFDAIKKVSP

Sequences:

>Translated_599_residues
MPTVENTILICAGGACISAGEKSVKDALQDLLKEYALDSVVKIIETGCMGACDLGPIIIVYPEGIFYQKLTPENVRRVVE
EHLLKGRIVEDMLYKGEFGEVKAKPQEELPFFTKQVKIATRNLGVIDPLSIDEYIARDGYFALHKAIKEMTPEEVISEVK
KSELRGRGGAGFPTGLKWEFVRKAKGDIKYVICNADEGDPGAFMDRAILEGDPHSVVEAMTIAAYAVGAQKGFIYVRAEY
PLAIERFSKALETAREYGFLGKNILGTGFSFDIEIRIGAGAFVCGEETALINSIEGKRGVPRVKPPYPATYGLWGKPTLI
NNVETYANIAPIILHGGDWFAQYGVEGSRGTKVFALAGKIKNTGLVEVPMGITLRELIYEIGGGVPDGKKLKAVQTGGPS
GGCIPEEHLDTPITYDNLKKLGTIIGSGGMIVMDEDDCMVDVAKFFLEFTVEESCGQCTPCRDGTKKMLEILEKITAGEG
TEEDLEVLKELGEHIMKTSLCGLGQTAPQPVVSTMRYFWDEYIAHVKEKRCPAKRCKELTRVVIDEEKCVGCTACARVCP
VNGIIGDIRKPHRIDPEICTRCGSCIEVCRFDAIKKVSP
>Mature_598_residues
PTVENTILICAGGACISAGEKSVKDALQDLLKEYALDSVVKIIETGCMGACDLGPIIIVYPEGIFYQKLTPENVRRVVEE
HLLKGRIVEDMLYKGEFGEVKAKPQEELPFFTKQVKIATRNLGVIDPLSIDEYIARDGYFALHKAIKEMTPEEVISEVKK
SELRGRGGAGFPTGLKWEFVRKAKGDIKYVICNADEGDPGAFMDRAILEGDPHSVVEAMTIAAYAVGAQKGFIYVRAEYP
LAIERFSKALETAREYGFLGKNILGTGFSFDIEIRIGAGAFVCGEETALINSIEGKRGVPRVKPPYPATYGLWGKPTLIN
NVETYANIAPIILHGGDWFAQYGVEGSRGTKVFALAGKIKNTGLVEVPMGITLRELIYEIGGGVPDGKKLKAVQTGGPSG
GCIPEEHLDTPITYDNLKKLGTIIGSGGMIVMDEDDCMVDVAKFFLEFTVEESCGQCTPCRDGTKKMLEILEKITAGEGT
EEDLEVLKELGEHIMKTSLCGLGQTAPQPVVSTMRYFWDEYIAHVKEKRCPAKRCKELTRVVIDEEKCVGCTACARVCPV
NGIIGDIRKPHRIDPEICTRCGSCIEVCRFDAIKKVSP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=370, Percent_Identity=42.972972972973, Blast_Score=299, Evalue=5e-81,
Organism=Homo sapiens, GI260656005, Length=370, Percent_Identity=42.972972972973, Blast_Score=299, Evalue=5e-81,
Organism=Escherichia coli, GI1788620, Length=404, Percent_Identity=42.5742574257426, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17531727, Length=417, Percent_Identity=39.568345323741, Blast_Score=294, Evalue=9e-80,
Organism=Drosophila melanogaster, GI45550452, Length=367, Percent_Identity=42.7792915531335, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI19920794, Length=369, Percent_Identity=42.5474254742547, Blast_Score=295, Evalue=8e-80,
Organism=Drosophila melanogaster, GI24653819, Length=375, Percent_Identity=34.4, Blast_Score=219, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24653817, Length=347, Percent_Identity=31.9884726224784, Blast_Score=187, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 65506; Mature: 65374

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS01099 COMPLEX1_24K ; PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTVENTILICAGGACISAGEKSVKDALQDLLKEYALDSVVKIIETGCMGACDLGPIIIV
CCCCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
YPEGIFYQKLTPENVRRVVEEHLLKGRIVEDMLYKGEFGEVKAKPQEELPFFTKQVKIAT
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHEEEE
RNLGVIDPLSIDEYIARDGYFALHKAIKEMTPEEVISEVKKSELRGRGGAGFPTGLKWEF
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
VRKAKGDIKYVICNADEGDPGAFMDRAILEGDPHSVVEAMTIAAYAVGAQKGFIYVRAEY
HHHCCCCEEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECC
PLAIERFSKALETAREYGFLGKNILGTGFSFDIEIRIGAGAFVCGEETALINSIEGKRGV
CHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEEEEECCCEEEECCCHHHHHCCCCCCCC
PRVKPPYPATYGLWGKPTLINNVETYANIAPIILHGGDWFAQYGVEGSRGTKVFALAGKI
CCCCCCCCCCCCCCCCCHHHCCHHHHHCCCEEEEECCCHHHHCCCCCCCCCEEEEEECCC
KNTGLVEVPMGITLRELIYEIGGGVPDGKKLKAVQTGGPSGGCIPEEHLDTPITYDNLKK
CCCCEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCCHHHHHH
LGTIIGSGGMIVMDEDDCMVDVAKFFLEFTVEESCGQCTPCRDGTKKMLEILEKITAGEG
HHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
TEEDLEVLKELGEHIMKTSLCGLGQTAPQPVVSTMRYFWDEYIAHVKEKRCPAKRCKELT
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RVVIDEEKCVGCTACARVCPVNGIIGDIRKPHRIDPEICTRCGSCIEVCRFDAIKKVSP
HHHHCCCCCCCHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PTVENTILICAGGACISAGEKSVKDALQDLLKEYALDSVVKIIETGCMGACDLGPIIIV
CCCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
YPEGIFYQKLTPENVRRVVEEHLLKGRIVEDMLYKGEFGEVKAKPQEELPFFTKQVKIAT
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHEEEE
RNLGVIDPLSIDEYIARDGYFALHKAIKEMTPEEVISEVKKSELRGRGGAGFPTGLKWEF
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
VRKAKGDIKYVICNADEGDPGAFMDRAILEGDPHSVVEAMTIAAYAVGAQKGFIYVRAEY
HHHCCCCEEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECC
PLAIERFSKALETAREYGFLGKNILGTGFSFDIEIRIGAGAFVCGEETALINSIEGKRGV
CHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEEEEECCCEEEECCCHHHHHCCCCCCCC
PRVKPPYPATYGLWGKPTLINNVETYANIAPIILHGGDWFAQYGVEGSRGTKVFALAGKI
CCCCCCCCCCCCCCCCCHHHCCHHHHHCCCEEEEECCCHHHHCCCCCCCCCEEEEEECCC
KNTGLVEVPMGITLRELIYEIGGGVPDGKKLKAVQTGGPSGGCIPEEHLDTPITYDNLKK
CCCCEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCCCHHHHHH
LGTIIGSGGMIVMDEDDCMVDVAKFFLEFTVEESCGQCTPCRDGTKKMLEILEKITAGEG
HHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
TEEDLEVLKELGEHIMKTSLCGLGQTAPQPVVSTMRYFWDEYIAHVKEKRCPAKRCKELT
CHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RVVIDEEKCVGCTACARVCPVNGIIGDIRKPHRIDPEICTRCGSCIEVCRFDAIKKVSP
HHHHCCCCCCCHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]