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The map label for this gene is wcaJ [H]

Identifier: 239616579

GI number: 239616579

Start: 191354

End: 192274

Strand: Direct

Name: wcaJ [H]

Synonym: Kole_0171

Alternate gene names: 239616579

Gene position: 191354-192274 (Clockwise)

Preceding gene: 239616576

Following gene: 239616588

Centisome position: 8.31

GC content: 40.83

Gene sequence:

>921_bases
ATGTTTTTTTATGTGATCGATTTTCTATTTTCGATTCCCTTGATAACTAATAGTGTTGAGGATTTTTTACTGGCATTTCT
AATCAGCGGATTCGTGTCTCTTTCAATGAGGGGGTATGAATCACAGATTATCCTTGATCCCAAAGAAAGAAAAATTTCTT
TCTTTGGGTCATTGGTGATGGTAACGATTCTGTTTTTTGTTTTTACCGGATCTGATATAATCATTCTTTTGCCTGCGTTA
TTATCCGCACTATTCCGCTATACAATCCTCTTTCGGTTCAAAAGATTTTTCAAGCTCAGACCAATGACCGTTGATCCCGG
AACAGTCGAAGAAGACTTTCAGGAGCTTGTCCACGTAAAAACTTATGATGGTTTTAAGAGATTTCTCGACTTTGTTTTTG
GAGTAGTTTTTCTGATTCTTTTGACTCCAATCTTTCTCATAATAGCCGTACTAATCCTGATCACTTCCGGAAGACCCGTG
TTCATAACACAGGATCGCATGGGAAGAAATGAGAAGGTATTCAAAATGCTCAAGTTCAGAACGTTCACGAACGGCGAACA
TCACAAGAGAATAACCAGGGTTGGCAAAATTTTGCGCCCACTGAGGCTCGATGAGCTCCCCCAGATTATCAACATCATTC
TCGGCGAAATGAGCCTGGTTGGCCCCAGACCAGAACTCAAAGCCTTTCACGAGATGGGTAAAAAACACGTTCCAAACTAT
AAGCTTCGTTTACTGGTGAGACCCGGTCTCACCGGCTGGGCACAGATTAACTATAAATACACTACAACCCCTGAGGAATA
CATGATAAAAACCTCATACGATCTGTACTACGTTTTGAATCGCTCTCTGAGACTGGATATCCTGTGCTTGCTCAAAACTC
CCTACGCTGTAATAAAATCTCTGGTGTCTAAAGATTTCTAG

Upstream 100 bases:

>100_bases
ACACCGTTAAATGCGACAATTCCTAACCGTTTTCATTTTACCTCAGTATCTTCAAATTTCCCAAAAATCATGCGACAATA
ATAGCGATGGGAGAGATGTC

Downstream 100 bases:

>100_bases
GCTGCTAATAAAAAAATCGGGGACATTTGATAAAGTGTCCCCGATTATCTATCTCCCGCTCACATTGAGCTCAATTTACT
TCTTCAAGTTCTTTCTTTCG

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MFFYVIDFLFSIPLITNSVEDFLLAFLISGFVSLSMRGYESQIILDPKERKISFFGSLVMVTILFFVFTGSDIIILLPAL
LSALFRYTILFRFKRFFKLRPMTVDPGTVEEDFQELVHVKTYDGFKRFLDFVFGVVFLILLTPIFLIIAVLILITSGRPV
FITQDRMGRNEKVFKMLKFRTFTNGEHHKRITRVGKILRPLRLDELPQIINIILGEMSLVGPRPELKAFHEMGKKHVPNY
KLRLLVRPGLTGWAQINYKYTTTPEEYMIKTSYDLYYVLNRSLRLDILCLLKTPYAVIKSLVSKDF

Sequences:

>Translated_306_residues
MFFYVIDFLFSIPLITNSVEDFLLAFLISGFVSLSMRGYESQIILDPKERKISFFGSLVMVTILFFVFTGSDIIILLPAL
LSALFRYTILFRFKRFFKLRPMTVDPGTVEEDFQELVHVKTYDGFKRFLDFVFGVVFLILLTPIFLIIAVLILITSGRPV
FITQDRMGRNEKVFKMLKFRTFTNGEHHKRITRVGKILRPLRLDELPQIINIILGEMSLVGPRPELKAFHEMGKKHVPNY
KLRLLVRPGLTGWAQINYKYTTTPEEYMIKTSYDLYYVLNRSLRLDILCLLKTPYAVIKSLVSKDF
>Mature_306_residues
MFFYVIDFLFSIPLITNSVEDFLLAFLISGFVSLSMRGYESQIILDPKERKISFFGSLVMVTILFFVFTGSDIIILLPAL
LSALFRYTILFRFKRFFKLRPMTVDPGTVEEDFQELVHVKTYDGFKRFLDFVFGVVFLILLTPIFLIIAVLILITSGRPV
FITQDRMGRNEKVFKMLKFRTFTNGEHHKRITRVGKILRPLRLDELPQIINIILGEMSLVGPRPELKAFHEMGKKHVPNY
KLRLLVRPGLTGWAQINYKYTTTPEEYMIKTSYDLYYVLNRSLRLDILCLLKTPYAVIKSLVSKDF

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=175, Percent_Identity=38.8571428571429, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 35700; Mature: 35700

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFYVIDFLFSIPLITNSVEDFLLAFLISGFVSLSMRGYESQIILDPKERKISFFGSLVM
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHH
VTILFFVFTGSDIIILLPALLSALFRYTILFRFKRFFKLRPMTVDPGTVEEDFQELVHVK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
TYDGFKRFLDFVFGVVFLILLTPIFLIIAVLILITSGRPVFITQDRMGRNEKVFKMLKFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
TFTNGEHHKRITRVGKILRPLRLDELPQIINIILGEMSLVGPRPELKAFHEMGKKHVPNY
HCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
KLRLLVRPGLTGWAQINYKYTTTPEEYMIKTSYDLYYVLNRSLRLDILCLLKTPYAVIKS
EEEEEECCCCCCEEEEEEEEECCHHHHEEECCCCEEEEECCCCHHHHHHHHCCCHHHHHH
LVSKDF
HHHCCC
>Mature Secondary Structure
MFFYVIDFLFSIPLITNSVEDFLLAFLISGFVSLSMRGYESQIILDPKERKISFFGSLVM
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHH
VTILFFVFTGSDIIILLPALLSALFRYTILFRFKRFFKLRPMTVDPGTVEEDFQELVHVK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
TYDGFKRFLDFVFGVVFLILLTPIFLIIAVLILITSGRPVFITQDRMGRNEKVFKMLKFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
TFTNGEHHKRITRVGKILRPLRLDELPQIINIILGEMSLVGPRPELKAFHEMGKKHVPNY
HCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
KLRLLVRPGLTGWAQINYKYTTTPEEYMIKTSYDLYYVLNRSLRLDILCLLKTPYAVIKS
EEEEEECCCCCCEEEEEEEEECCHHHHEEECCCCEEEEECCCCHHHHHHHHCCCHHHHHH
LVSKDF
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]