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The map label for this gene is hisJ [H]

Identifier: 239616531

GI number: 239616531

Start: 139136

End: 139858

Strand: Direct

Name: hisJ [H]

Synonym: Kole_0123

Alternate gene names: 239616531

Gene position: 139136-139858 (Clockwise)

Preceding gene: 239616530

Following gene: 239616532

Centisome position: 6.04

GC content: 44.4

Gene sequence:

>723_bases
ATGAGGAAACTGAGTCTGGTTATCTTGCTTGTTGCCCTGGCTGCGTTTGCTTTTGGAAAGACTTACGTTGTAGGAACGAG
TGCTGATTTTCCGCCGTTCGAGTACGTGGAAAACGGTGAGTTCAAAGGGTTCGACATGGACCTGATAAGAGCGATAGCTG
ACGAAATGGGATTCAATATCAAGATTGTTGATATGAGCTTCGATTCCCTTATCGCTGCTCTCGTTTCCGGAAACCTTGAC
ATCGTTATCGCGGGAATGACCATCACTGCGGAACGTGAAGCGGTTGTCGATTTTTCCAAACCTTATTGGACGGCTGACCA
GAGCATTATAGTTAAGGAAGATTCTGACTACACAGTTACCGTTCTCTTTGGGAAGCATGATATTGGTGTTCAGACCGGTA
CAACCGGTGACCTGTGGGTTGAAGAGAATCTTGTAAAGACAAAGATCCTCACTGGAAAGTTCAAGAGATATGATACCTTT
GTTCTTGCACTCACCGACCTTATCAACGGCAACGTGGACGCAATCGTTCTGGATTCACCGGTTGCAGAGAGGTTTGCTGA
AACAAGGCCGGTGAAAATAGTTGGTATAATCGTTACCGGTGAAGAATACGGTATAGCCGTAAGAGAAGGAAACAAGGAGC
TTCTAAATCTGATAAACGAAGGAATTCAACGTTTGAAGGAAAACGGAAAACTTGGAGAAATCGTTGAAAAATACTTCAAG
TGA

Upstream 100 bases:

>100_bases
CCTTTAGCTTTTCGAACGATGAATTCTCTTTTGCGATAGGCTGGAGCTTTAAACTCTTTTAGAACAGCCTTTTATATCCA
TTGATTGGAGGGGTGAGATT

Downstream 100 bases:

>100_bases
TTACGAAAGGCGGTAGAGAGCTATCGAAAAGATAAACATCATTATCGAGGCTTTACCGGCTCTTCTGAACGGCACATGGA
TGACACTGAAATTGACCTTC

Product: extracellular solute-binding protein family 3

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MRKLSLVILLVALAAFAFGKTYVVGTSADFPPFEYVENGEFKGFDMDLIRAIADEMGFNIKIVDMSFDSLIAALVSGNLD
IVIAGMTITAEREAVVDFSKPYWTADQSIIVKEDSDYTVTVLFGKHDIGVQTGTTGDLWVEENLVKTKILTGKFKRYDTF
VLALTDLINGNVDAIVLDSPVAERFAETRPVKIVGIIVTGEEYGIAVREGNKELLNLINEGIQRLKENGKLGEIVEKYFK

Sequences:

>Translated_240_residues
MRKLSLVILLVALAAFAFGKTYVVGTSADFPPFEYVENGEFKGFDMDLIRAIADEMGFNIKIVDMSFDSLIAALVSGNLD
IVIAGMTITAEREAVVDFSKPYWTADQSIIVKEDSDYTVTVLFGKHDIGVQTGTTGDLWVEENLVKTKILTGKFKRYDTF
VLALTDLINGNVDAIVLDSPVAERFAETRPVKIVGIIVTGEEYGIAVREGNKELLNLINEGIQRLKENGKLGEIVEKYFK
>Mature_240_residues
MRKLSLVILLVALAAFAFGKTYVVGTSADFPPFEYVENGEFKGFDMDLIRAIADEMGFNIKIVDMSFDSLIAALVSGNLD
IVIAGMTITAEREAVVDFSKPYWTADQSIIVKEDSDYTVTVLFGKHDIGVQTGTTGDLWVEENLVKTKILTGKFKRYDTF
VLALTDLINGNVDAIVLDSPVAERFAETRPVKIVGIIVTGEEYGIAVREGNKELLNLINEGIQRLKENGKLGEIVEKYFK

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=250, Percent_Identity=34.8, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1787088, Length=250, Percent_Identity=33.6, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787031, Length=241, Percent_Identity=34.0248962655602, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788228, Length=230, Percent_Identity=31.304347826087, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788649, Length=250, Percent_Identity=27.6, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=27.1929824561404, Blast_Score=84, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25144707, Length=96, Percent_Identity=38.5416666666667, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26515; Mature: 26515

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLSLVILLVALAAFAFGKTYVVGTSADFPPFEYVENGEFKGFDMDLIRAIADEMGFNI
CCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCEE
KIVDMSFDSLIAALVSGNLDIVIAGMTITAEREAVVDFSKPYWTADQSIIVKEDSDYTVT
EEEECCHHHHHHHHHCCCCEEEEEEEEEEECCCEEEECCCCCCCCCCEEEEEECCCEEEE
VLFGKHDIGVQTGTTGDLWVEENLVKTKILTGKFKRYDTFVLALTDLINGNVDAIVLDSP
EEEECCCCCEECCCCCCEEEECCHHEEHHHCCCHHHHHHHHEEHHHHHCCCCCEEEECCH
VAERFAETRPVKIVGIIVTGEEYGIAVREGNKELLNLINEGIQRLKENGKLGEIVEKYFK
HHHHHHCCCCEEEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MRKLSLVILLVALAAFAFGKTYVVGTSADFPPFEYVENGEFKGFDMDLIRAIADEMGFNI
CCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCEE
KIVDMSFDSLIAALVSGNLDIVIAGMTITAEREAVVDFSKPYWTADQSIIVKEDSDYTVT
EEEECCHHHHHHHHHCCCCEEEEEEEEEEECCCEEEECCCCCCCCCCEEEEEECCCEEEE
VLFGKHDIGVQTGTTGDLWVEENLVKTKILTGKFKRYDTFVLALTDLINGNVDAIVLDSP
EEEECCCCCEECCCCCCEEEECCHHEEHHHCCCHHHHHHHHEEHHHHHCCCCCEEEECCH
VAERFAETRPVKIVGIIVTGEEYGIAVREGNKELLNLINEGIQRLKENGKLGEIVEKYFK
HHHHHHCCCCEEEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]