Click here to switch to the map view.

The map label for this gene is rpfG [H]

Identifier: 239616521

GI number: 239616521

Start: 127358

End: 128551

Strand: Direct

Name: rpfG [H]

Synonym: Kole_0111

Alternate gene names: 239616521

Gene position: 127358-128551 (Clockwise)

Preceding gene: 239616520

Following gene: 239616522

Centisome position: 5.53

GC content: 39.61

Gene sequence:

>1194_bases
ATGAAGAAAAGGCGCTTGCCATCGGCTGTGTTGGGTACATCAAGAAACCTTTTTCCTGAGAATTTCATACCAGAAATAGG
GAAGGTGATAAACATGAAAATTCTGGCTGTTGATGATAACAAAGAAAATATCTATATGTTAGAGACTCTGTTCAAGAGCT
ATGGTTATGAAGTTGAATCTTCCCAGAATGGTGTTGAAGCTCTGGAGAAATTGGCCGAACAGGATTTTGACGTGGTAATT
TCCGATATTTTGATGCCCGAGATGGACGGCTTTCAATTATGTCGCAGGGTGAAAATGGATGATAGATTAAAAGAAATCCC
TTTTATACTTTATACCGCTGCCTACACCGATCCAGAAGATAGAAAATTTGCCCTCAGCCTTGGAGTCGATAGGTTTATTA
TCAAGCCATTAGAACCAGATGCTTTTGTTAAGATAGTAAGAGAAGTTATTCAGAACCATAAAGTGAAACCTTTAAACAAC
AACAAGGTAGTGAATAAAGAAGAGATTGTATATCTTAAGAACTACAACGAGAGATTGATCAAGAAATTGGAAGATGGAAT
GCTTCAGTTAGAAGAAAAAAATAAAGAACTCGAACAAAGCTATAAAAAGGTACAGAGAACCCTGGATTCCGTTGTTGAAA
CAATAGGATTGGTAATTGAAATGAGAGATGCCTACACTTCTGGTCATCAACGACGCGTAAGCCAGCTTGCCTGCGCTATT
GCCAGAGAAATGAACCTGTCAGAAGAACAAATTGAGGCTGTTCGTATAGCTGGCCTTCTACATGATATTGGCAAGATATC
CATACCGACAGATATTTTGAACAAGCCCGACAAACTGAATAAATTTGAACTCGGAATTGTAAGAAACCATCCACAAACTG
CATTTGAAATATTAAAAGACGTGGAATTTTCCCAGCCCATAGCTGAGATTGTGCTCCAGCATCACGAACGAATAGATGGA
TCTGGTTATCCTAATGGCTTGAAAGGTAACCAAATTCTCACAGAAGCGAAGATACTAGCAGTGTCAGATGTTGTTGAAGC
CATGGCGTTCCACAGACCTTATCGTCCGGCACTTGGTATAGCTAAAGCTTTAGAGGAGATCACCACAAATTCAGGTAACC
TCTACGACCCTGAGACTGTAAAAGCTTGCATAGCAGTGTTCTCGCGGGGATTTGATTTTGAAAAAGAAAGGTAA

Upstream 100 bases:

>100_bases
AAATCTGTGGAGGAGAATAATCATGAAGAAAAAAGTACTGGTGATAGATGATAACGAACAGAATCTGTTATCGCTGTAAC
TTCTTAAGCTATGCCAGCAG

Downstream 100 bases:

>100_bases
AAAGTTTGCCTTTTCCAGCCGAACTCTCCCCAGCCTCGATACCTCATCATATGCCTCCTGAGTTTCATTTTCACTCTCTG
TCCCACATTTCCGAAGCATT

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKKRRLPSAVLGTSRNLFPENFIPEIGKVINMKILAVDDNKENIYMLETLFKSYGYEVESSQNGVEALEKLAEQDFDVVI
SDILMPEMDGFQLCRRVKMDDRLKEIPFILYTAAYTDPEDRKFALSLGVDRFIIKPLEPDAFVKIVREVIQNHKVKPLNN
NKVVNKEEIVYLKNYNERLIKKLEDGMLQLEEKNKELEQSYKKVQRTLDSVVETIGLVIEMRDAYTSGHQRRVSQLACAI
AREMNLSEEQIEAVRIAGLLHDIGKISIPTDILNKPDKLNKFELGIVRNHPQTAFEILKDVEFSQPIAEIVLQHHERIDG
SGYPNGLKGNQILTEAKILAVSDVVEAMAFHRPYRPALGIAKALEEITTNSGNLYDPETVKACIAVFSRGFDFEKER

Sequences:

>Translated_397_residues
MKKRRLPSAVLGTSRNLFPENFIPEIGKVINMKILAVDDNKENIYMLETLFKSYGYEVESSQNGVEALEKLAEQDFDVVI
SDILMPEMDGFQLCRRVKMDDRLKEIPFILYTAAYTDPEDRKFALSLGVDRFIIKPLEPDAFVKIVREVIQNHKVKPLNN
NKVVNKEEIVYLKNYNERLIKKLEDGMLQLEEKNKELEQSYKKVQRTLDSVVETIGLVIEMRDAYTSGHQRRVSQLACAI
AREMNLSEEQIEAVRIAGLLHDIGKISIPTDILNKPDKLNKFELGIVRNHPQTAFEILKDVEFSQPIAEIVLQHHERIDG
SGYPNGLKGNQILTEAKILAVSDVVEAMAFHRPYRPALGIAKALEEITTNSGNLYDPETVKACIAVFSRGFDFEKER
>Mature_397_residues
MKKRRLPSAVLGTSRNLFPENFIPEIGKVINMKILAVDDNKENIYMLETLFKSYGYEVESSQNGVEALEKLAEQDFDVVI
SDILMPEMDGFQLCRRVKMDDRLKEIPFILYTAAYTDPEDRKFALSLGVDRFIIKPLEPDAFVKIVREVIQNHKVKPLNN
NKVVNKEEIVYLKNYNERLIKKLEDGMLQLEEKNKELEQSYKKVQRTLDSVVETIGLVIEMRDAYTSGHQRRVSQLACAI
AREMNLSEEQIEAVRIAGLLHDIGKISIPTDILNKPDKLNKFELGIVRNHPQTAFEILKDVEFSQPIAEIVLQHHERIDG
SGYPNGLKGNQILTEAKILAVSDVVEAMAFHRPYRPALGIAKALEEITTNSGNLYDPETVKACIAVFSRGFDFEKER

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=110, Percent_Identity=37.2727272727273, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI145693157, Length=126, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788713, Length=126, Percent_Identity=30.952380952381, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788550, Length=124, Percent_Identity=36.2903225806452, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790299, Length=132, Percent_Identity=31.0606060606061, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=30.0813008130081, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789149, Length=109, Percent_Identity=30.2752293577982, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 45323; Mature: 45323

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRRLPSAVLGTSRNLFPENFIPEIGKVINMKILAVDDNKENIYMLETLFKSYGYEVES
CCCCCCCHHHHCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCCCCC
SQNGVEALEKLAEQDFDVVISDILMPEMDGFQLCRRVKMDDRLKEIPFILYTAAYTDPED
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCC
RKFALSLGVDRFIIKPLEPDAFVKIVREVIQNHKVKPLNNNKVVNKEEIVYLKNYNERLI
CEEEEEECCCHHEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEECCCHHHH
KKLEDGMLQLEEKNKELEQSYKKVQRTLDSVVETIGLVIEMRDAYTSGHQRRVSQLACAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AREMNLSEEQIEAVRIAGLLHDIGKISIPTDILNKPDKLNKFELGIVRNHPQTAFEILKD
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCEEEEEECCCCHHHHHHHHH
VEFSQPIAEIVLQHHERIDGSGYPNGLKGNQILTEAKILAVSDVVEAMAFHRPYRPALGI
CHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AKALEEITTNSGNLYDPETVKACIAVFSRGFDFEKER
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKKRRLPSAVLGTSRNLFPENFIPEIGKVINMKILAVDDNKENIYMLETLFKSYGYEVES
CCCCCCCHHHHCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCCCCC
SQNGVEALEKLAEQDFDVVISDILMPEMDGFQLCRRVKMDDRLKEIPFILYTAAYTDPED
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCC
RKFALSLGVDRFIIKPLEPDAFVKIVREVIQNHKVKPLNNNKVVNKEEIVYLKNYNERLI
CEEEEEECCCHHEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEECCCHHHH
KKLEDGMLQLEEKNKELEQSYKKVQRTLDSVVETIGLVIEMRDAYTSGHQRRVSQLACAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AREMNLSEEQIEAVRIAGLLHDIGKISIPTDILNKPDKLNKFELGIVRNHPQTAFEILKD
HHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCEEEEEECCCCHHHHHHHHH
VEFSQPIAEIVLQHHERIDGSGYPNGLKGNQILTEAKILAVSDVVEAMAFHRPYRPALGI
CHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AKALEEITTNSGNLYDPETVKACIAVFSRGFDFEKER
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]