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The map label for this gene is pleC [H]

Identifier: 239616520

GI number: 239616520

Start: 125629

End: 127308

Strand: Direct

Name: pleC [H]

Synonym: Kole_0110

Alternate gene names: 239616520

Gene position: 125629-127308 (Clockwise)

Preceding gene: 239616511

Following gene: 239616521

Centisome position: 5.46

GC content: 36.25

Gene sequence:

>1680_bases
ATGAATAAGTTGATGAACGTCTTCAGAATGAGTCTGTTTGTTTCTTTGCTGATCATATTTCTCGCTGTGACCTTTGGGAT
TTACCTGCCAATGAAAAATGAACTTACAAAAGAACTCAATGAAAAATTTTCAAACAACGCTAAATTATCCAAGAAGGCCA
TTGACAATTTTCTTAAGCGTTGTATTCAGGGAGCAGAAAGCCTTTCTAGCCGTACTATGATCAGAAAGATGATAGTTGAA
TATCTCAGTGGAAACATCACTTTCAAAGAACTTCAAAAATATACTGCACCGAAATATCTCGATGGTGCTAATGTTCTCCA
AAACTTAAAAAGAGCCACACGGATTGTTTCAGGAAACATCATTGCTTCTGTTGGAAGCTTAGACAATTTAGATATTGGAA
AAGCAATAAGTTGTGAAGACGTGTCAGAAACAACTTTAGATATAGAATTTGAGAATCAATTTATTGTTGTCAATGTGTGT
TCTCCAATACGTGAAAATACTACTCACAATTTTGTTTTTATGACCTCTTCGAGAGGACGCAATGGCGAAAATGCCACGGT
TATAGGTCATGATTTAGTTTCTTTTGACATGACAAAACTCTTAGATGAGATCAATACAGGAAATATTGTATATGAGATCT
ATATCGCAACTAATTCAAAATACATCTCATTAAAAGGAAAAATATTTGACGTTCTCAAAGACGACACTTTGAGTTCAGAT
GGGAAAACAACGCGGTACACCATGAAATTAAGAGATACAGACGCTTTTCTCGTTGCAAGTGTACCCAATAATATTATCTA
TACTCGCATAAAAAAAATATCCCTAACCATTTTCATTGTTATTGCTGCTGCATTTCTGATTGTTGTTCTCTTAATACATT
TTACGATTCTCACAAACGCAAGGAAGATGATAAAAACCATAGAACAAGATAATATCAAGATCAAAGAGTTAGAACGTCTT
AAATCTGAATTTCTTGCTACCATGAGTCATGAGTTGAGAACCCCTCTCAACTCCATTACTGGGTTCGTTAGCATGATTTT
ACAGGGCATTTCAGGAGAAATCAACGAAGAACAAAGGAAACAACTCTCTATGGTTCACAGTTCTTCAATACATCTCTTAA
ATCTGATAAACGATATTCTGGATATCTCCAAGATTGAAGCTGGTAGAATGAGTTTTTCTAAGGAAAGATTCAACATTAAA
GACGTTATTTCTGAAGTTGTACAAAATGTTTCGCTGTTGATTTCTAAAAAAGAAGTGAAGTTAATAACAGAAATACCTGA
TGAGATTCCAGAGATCTACAGTGATAAAAGGAGGGTTTTCCAGATTCTCCTGAATCTGGTCAGTAATGCGATTAAATTCA
CGGAGAAGGGCGAGGTAAGGATAAAATGCGAGGTAGATGGAAATAAACTGAAAATCAGTGTTTCTGATACAGGAAAAGGG
ATAAAGGAAGAAGATATGGAAACATTATTTGAAGCTTTTCGACAGATAAGTGGTTCGGCACGGCGCAGGTACCAAGGTGC
TGGACTGGGGCTCTATCTCTCCAAAAAGCTTGTGACATTGTTGGGCGGTGAGATCTGGGTTGAGAGCGAATACGGTAAAG
GTTCTACATTTACATTCACCCTGCCGCTCAAATCTGTGGAGGAGAATAATCATGAAGAAAAAAGTACTGGTGATAGATGA

Upstream 100 bases:

>100_bases
ATTAAATACTCACTAAGAACATCATTTTGACAAGATCATCATTGAAGGTTGAAGCATTTTAAGGAGGAATAATGGAAATG
TTTGATTTCCTGGAGGCGTT

Downstream 100 bases:

>100_bases
TAACGAACAGAATCTGTTATCGCTGTAACTTCTTAAGCTATGCCAGCAGATGAAGAAAAGGCGCTTGCCATCGGCTGTGT
TGGGTACATCAAGAAACCTT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MNKLMNVFRMSLFVSLLIIFLAVTFGIYLPMKNELTKELNEKFSNNAKLSKKAIDNFLKRCIQGAESLSSRTMIRKMIVE
YLSGNITFKELQKYTAPKYLDGANVLQNLKRATRIVSGNIIASVGSLDNLDIGKAISCEDVSETTLDIEFENQFIVVNVC
SPIRENTTHNFVFMTSSRGRNGENATVIGHDLVSFDMTKLLDEINTGNIVYEIYIATNSKYISLKGKIFDVLKDDTLSSD
GKTTRYTMKLRDTDAFLVASVPNNIIYTRIKKISLTIFIVIAAAFLIVVLLIHFTILTNARKMIKTIEQDNIKIKELERL
KSEFLATMSHELRTPLNSITGFVSMILQGISGEINEEQRKQLSMVHSSSIHLLNLINDILDISKIEAGRMSFSKERFNIK
DVISEVVQNVSLLISKKEVKLITEIPDEIPEIYSDKRRVFQILLNLVSNAIKFTEKGEVRIKCEVDGNKLKISVSDTGKG
IKEEDMETLFEAFRQISGSARRRYQGAGLGLYLSKKLVTLLGGEIWVESEYGKGSTFTFTLPLKSVEENNHEEKSTGDR

Sequences:

>Translated_559_residues
MNKLMNVFRMSLFVSLLIIFLAVTFGIYLPMKNELTKELNEKFSNNAKLSKKAIDNFLKRCIQGAESLSSRTMIRKMIVE
YLSGNITFKELQKYTAPKYLDGANVLQNLKRATRIVSGNIIASVGSLDNLDIGKAISCEDVSETTLDIEFENQFIVVNVC
SPIRENTTHNFVFMTSSRGRNGENATVIGHDLVSFDMTKLLDEINTGNIVYEIYIATNSKYISLKGKIFDVLKDDTLSSD
GKTTRYTMKLRDTDAFLVASVPNNIIYTRIKKISLTIFIVIAAAFLIVVLLIHFTILTNARKMIKTIEQDNIKIKELERL
KSEFLATMSHELRTPLNSITGFVSMILQGISGEINEEQRKQLSMVHSSSIHLLNLINDILDISKIEAGRMSFSKERFNIK
DVISEVVQNVSLLISKKEVKLITEIPDEIPEIYSDKRRVFQILLNLVSNAIKFTEKGEVRIKCEVDGNKLKISVSDTGKG
IKEEDMETLFEAFRQISGSARRRYQGAGLGLYLSKKLVTLLGGEIWVESEYGKGSTFTFTLPLKSVEENNHEEKSTGDR
>Mature_559_residues
MNKLMNVFRMSLFVSLLIIFLAVTFGIYLPMKNELTKELNEKFSNNAKLSKKAIDNFLKRCIQGAESLSSRTMIRKMIVE
YLSGNITFKELQKYTAPKYLDGANVLQNLKRATRIVSGNIIASVGSLDNLDIGKAISCEDVSETTLDIEFENQFIVVNVC
SPIRENTTHNFVFMTSSRGRNGENATVIGHDLVSFDMTKLLDEINTGNIVYEIYIATNSKYISLKGKIFDVLKDDTLSSD
GKTTRYTMKLRDTDAFLVASVPNNIIYTRIKKISLTIFIVIAAAFLIVVLLIHFTILTNARKMIKTIEQDNIKIKELERL
KSEFLATMSHELRTPLNSITGFVSMILQGISGEINEEQRKQLSMVHSSSIHLLNLINDILDISKIEAGRMSFSKERFNIK
DVISEVVQNVSLLISKKEVKLITEIPDEIPEIYSDKRRVFQILLNLVSNAIKFTEKGEVRIKCEVDGNKLKISVSDTGKG
IKEEDMETLFEAFRQISGSARRRYQGAGLGLYLSKKLVTLLGGEIWVESEYGKGSTFTFTLPLKSVEENNHEEKSTGDR

Specific function: Member of the two-component regulatory system involved in the regulation of polar organelle development. PleC functions as a membrane-associated protein kinase that transfers phosphate to the response regulator pleD, leading to its activation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=255, Percent_Identity=40, Blast_Score=176, Evalue=5e-45,
Organism=Escherichia coli, GI145693157, Length=247, Percent_Identity=35.6275303643725, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI48994928, Length=233, Percent_Identity=36.0515021459227, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87081816, Length=227, Percent_Identity=37.0044052863436, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788713, Length=265, Percent_Identity=33.9622641509434, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786912, Length=263, Percent_Identity=27.7566539923954, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1790436, Length=278, Percent_Identity=29.8561151079137, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1786600, Length=257, Percent_Identity=26.8482490272374, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI87082128, Length=231, Percent_Identity=27.7056277056277, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788549, Length=241, Percent_Identity=28.6307053941909, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1788393, Length=244, Percent_Identity=23.7704918032787, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1786783, Length=235, Percent_Identity=25.9574468085106, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790346, Length=238, Percent_Identity=26.890756302521, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787894, Length=317, Percent_Identity=21.7665615141956, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788279, Length=234, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=226, Percent_Identity=23.4513274336283, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=79, Percent_Identity=41.7721518987342, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 63094; Mature: 63094

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLMNVFRMSLFVSLLIIFLAVTFGIYLPMKNELTKELNEKFSNNAKLSKKAIDNFLKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
CIQGAESLSSRTMIRKMIVEYLSGNITFKELQKYTAPKYLDGANVLQNLKRATRIVSGNI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
IASVGSLDNLDIGKAISCEDVSETTLDIEFENQFIVVNVCSPIRENTTHNFVFMTSSRGR
EEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCHHCCCCCEEEEEECCCCC
NGENATVIGHDLVSFDMTKLLDEINTGNIVYEIYIATNSKYISLKGKIFDVLKDDTLSSD
CCCCCEEEEHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCHHHHHHHCCCCCCC
GKTTRYTMKLRDTDAFLVASVPNNIIYTRIKKISLTIFIVIAAAFLIVVLLIHFTILTNA
CCCEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKMIKTIEQDNIKIKELERLKSEFLATMSHELRTPLNSITGFVSMILQGISGEINEEQRK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHH
QLSMVHSSSIHLLNLINDILDISKIEAGRMSFSKERFNIKDVISEVVQNVSLLISKKEVK
HHHHHHCCCHHHHHHHHHHHHHHHCCHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LITEIPDEIPEIYSDKRRVFQILLNLVSNAIKFTEKGEVRIKCEVDGNKLKISVSDTGKG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCC
IKEEDMETLFEAFRQISGSARRRYQGAGLGLYLSKKLVTLLGGEIWVESEYGKGSTFTFT
CCHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEEE
LPLKSVEENNHEEKSTGDR
EECHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNKLMNVFRMSLFVSLLIIFLAVTFGIYLPMKNELTKELNEKFSNNAKLSKKAIDNFLKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
CIQGAESLSSRTMIRKMIVEYLSGNITFKELQKYTAPKYLDGANVLQNLKRATRIVSGNI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
IASVGSLDNLDIGKAISCEDVSETTLDIEFENQFIVVNVCSPIRENTTHNFVFMTSSRGR
EEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCHHCCCCCEEEEEECCCCC
NGENATVIGHDLVSFDMTKLLDEINTGNIVYEIYIATNSKYISLKGKIFDVLKDDTLSSD
CCCCCEEEEHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCHHHHHHHCCCCCCC
GKTTRYTMKLRDTDAFLVASVPNNIIYTRIKKISLTIFIVIAAAFLIVVLLIHFTILTNA
CCCEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKMIKTIEQDNIKIKELERLKSEFLATMSHELRTPLNSITGFVSMILQGISGEINEEQRK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHH
QLSMVHSSSIHLLNLINDILDISKIEAGRMSFSKERFNIKDVISEVVQNVSLLISKKEVK
HHHHHHCCCHHHHHHHHHHHHHHHCCHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LITEIPDEIPEIYSDKRRVFQILLNLVSNAIKFTEKGEVRIKCEVDGNKLKISVSDTGKG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCC
IKEEDMETLFEAFRQISGSARRRYQGAGLGLYLSKKLVTLLGGEIWVESEYGKGSTFTFT
CCHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEEE
LPLKSVEENNHEEKSTGDR
EECHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8421698; 11259647 [H]