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The map label for this gene is pyk [H]

Identifier: 238925520

GI number: 238925520

Start: 3025892

End: 3027304

Strand: Reverse

Name: pyk [H]

Synonym: EUBREC_3176

Alternate gene names: 238925520

Gene position: 3027304-3025892 (Counterclockwise)

Preceding gene: 238925521

Following gene: 238925518

Centisome position: 87.76

GC content: 44.02

Gene sequence:

>1413_bases
ATGCGTAAAACAAAAATTATCTGTACTATTGGACCGGCCAGTGAACATGAGGATGTCCTCACCAGGATGATAAAGGCAGG
TATGGATGTTGCCAGATTGAATTTTTCTCATGGCTCACATGAGGAGCATCAGGGCAAAATAGATTTAATCAAGAAGGTAA
GACAGAAGCTTCATATGCCAATCGCAATCATGCTTGACACAAAGGGACCTGAGTATCGTATCAAGACCTTTGAGAATGGC
AAAATAGAGCTCAAGGATGGAGATACCTTCACACTTACCACTGACGATATTGTAGGAAATCAGGAGAGAGTATCAGTAAA
CTATGAGAATTTGATCAGGGATTTAAAGGTTGGTGACCGTGTGCTTGTTGCGAACGGAATCATCATTCTGCAGGTCCGAG
AGCTTAAAGGAAACGATGCAATCTGTGATGTTATCGCAGGCGGTACACTTTCAGACAGAAAGAGCATGAACTTCCCGAAT
AAGGTCATGAAGCATGCATATTTAAGCAAGCAGGACAAGGATGATCTGCTTTTTGGTATAAAAAACGAGGTGGATTATGT
TGCCGCATCATTCGTTTCTACAAAGCAGGATGTAGCTGATCTGAGAAGCTTTTTGGATGAAAACGGTGGAGAGGACATTG
ATATCATCGCAAAGATTGAGAACCGTTCAGGTGTAGATCATGTGGAGGAAATCTGTGAGATAGCAAATGGAATCATGATA
GCAAGAGGAGATCTCGGTGTTGAGATTCCGGGAGTTGAGGTACCGGCAATACAGAAGTATCTTATCAACAAGTGCCGTAT
GCTTGGTACACGCGTAATCACAGCAACAGAGATGCTTGAGTCCATGATTCACAATCCACGTCCTACAAGAGCTGAGCTTT
CGGATGTAGCCAATGCTGTATACGACGGCTCATCAGCTATTATGCTTTCGGGAGAGTCTGCAGCAGGTAAATATCCTGTA
GAGGCTGTAAAAAATATGGCAGAGACAGCAGAGTATACAGAAAAGCAGATAAACTACGGCAAGAGATTTGCCAATGCTGA
GTTCCAGACAAAAAGCATAGTCGATGCCATTTCACATGCTACATGTGCCATGGCAATAGATGTGGATGCGAAATGTATCG
TAGTCAGCTCGCTGACAGGCCGTACAGTGCGCATGGTCAGCCGTTTCCGCTGTCCTGTTGATATAATAGGCATGACAACC
TCTGAAAAGGCATGGCGCAAGCTGAACCTGAGCTGGGGTGTAAAGCCGGTTCTGTGCGAGGAATACAACTCAATGGAGGT
TATGTTCTACAATGCGATGAACGAGGCAAAGAGTGTCATGAAGCTCAAAAAGGGTGATCATATAGTGCTCACAGGAGGTC
TTATCAATGGTAAGACAGGCAACACCAATGTAATTAAGGTTGAGACAGTTTAG

Upstream 100 bases:

>100_bases
TTACTTTATTGTTGAACAAAATTGTCATATAATGACTTCGGGTGAGATTATATTTTTAAAAGAAAAGCTGATAGGGTGAT
CAGCATCAGGAGGAATATTA

Downstream 100 bases:

>100_bases
TACATTTATACTATTGATTAAATCGTATATATGTCATATAATTCTTAACGATTAATAAAAACAATAAATTTAAAATAAAC
AATTTCGAGATGAAGTTTGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MRKTKIICTIGPASEHEDVLTRMIKAGMDVARLNFSHGSHEEHQGKIDLIKKVRQKLHMPIAIMLDTKGPEYRIKTFENG
KIELKDGDTFTLTTDDIVGNQERVSVNYENLIRDLKVGDRVLVANGIIILQVRELKGNDAICDVIAGGTLSDRKSMNFPN
KVMKHAYLSKQDKDDLLFGIKNEVDYVAASFVSTKQDVADLRSFLDENGGEDIDIIAKIENRSGVDHVEEICEIANGIMI
ARGDLGVEIPGVEVPAIQKYLINKCRMLGTRVITATEMLESMIHNPRPTRAELSDVANAVYDGSSAIMLSGESAAGKYPV
EAVKNMAETAEYTEKQINYGKRFANAEFQTKSIVDAISHATCAMAIDVDAKCIVVSSLTGRTVRMVSRFRCPVDIIGMTT
SEKAWRKLNLSWGVKPVLCEEYNSMEVMFYNAMNEAKSVMKLKKGDHIVLTGGLINGKTGNTNVIKVETV

Sequences:

>Translated_470_residues
MRKTKIICTIGPASEHEDVLTRMIKAGMDVARLNFSHGSHEEHQGKIDLIKKVRQKLHMPIAIMLDTKGPEYRIKTFENG
KIELKDGDTFTLTTDDIVGNQERVSVNYENLIRDLKVGDRVLVANGIIILQVRELKGNDAICDVIAGGTLSDRKSMNFPN
KVMKHAYLSKQDKDDLLFGIKNEVDYVAASFVSTKQDVADLRSFLDENGGEDIDIIAKIENRSGVDHVEEICEIANGIMI
ARGDLGVEIPGVEVPAIQKYLINKCRMLGTRVITATEMLESMIHNPRPTRAELSDVANAVYDGSSAIMLSGESAAGKYPV
EAVKNMAETAEYTEKQINYGKRFANAEFQTKSIVDAISHATCAMAIDVDAKCIVVSSLTGRTVRMVSRFRCPVDIIGMTT
SEKAWRKLNLSWGVKPVLCEEYNSMEVMFYNAMNEAKSVMKLKKGDHIVLTGGLINGKTGNTNVIKVETV
>Mature_470_residues
MRKTKIICTIGPASEHEDVLTRMIKAGMDVARLNFSHGSHEEHQGKIDLIKKVRQKLHMPIAIMLDTKGPEYRIKTFENG
KIELKDGDTFTLTTDDIVGNQERVSVNYENLIRDLKVGDRVLVANGIIILQVRELKGNDAICDVIAGGTLSDRKSMNFPN
KVMKHAYLSKQDKDDLLFGIKNEVDYVAASFVSTKQDVADLRSFLDENGGEDIDIIAKIENRSGVDHVEEICEIANGIMI
ARGDLGVEIPGVEVPAIQKYLINKCRMLGTRVITATEMLESMIHNPRPTRAELSDVANAVYDGSSAIMLSGESAAGKYPV
EAVKNMAETAEYTEKQINYGKRFANAEFQTKSIVDAISHATCAMAIDVDAKCIVVSSLTGRTVRMVSRFRCPVDIIGMTT
SEKAWRKLNLSWGVKPVLCEEYNSMEVMFYNAMNEAKSVMKLKKGDHIVLTGGLINGKTGNTNVIKVETV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=494, Percent_Identity=40.2834008097166, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI33286422, Length=500, Percent_Identity=40, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI33286420, Length=500, Percent_Identity=40, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=39.918533604888, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=39.918533604888, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=41.9243986254296, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=41.9243986254296, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=40.5857740585774, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=40.5857740585774, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=38.9908256880734, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=45.3191489361702, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=37.0292887029289, Blast_Score=283, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17544584, Length=481, Percent_Identity=38.8773388773389, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=40.4517453798768, Blast_Score=294, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=40.4517453798768, Blast_Score=294, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=40.4517453798768, Blast_Score=294, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=40.4517453798768, Blast_Score=294, Evalue=7e-80,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=39.3004115226337, Blast_Score=311, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6324923, Length=485, Percent_Identity=38.7628865979381, Blast_Score=296, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24648964, Length=491, Percent_Identity=40.122199592668, Blast_Score=321, Evalue=5e-88,
Organism=Drosophila melanogaster, GI28571814, Length=491, Percent_Identity=40.122199592668, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI24648966, Length=413, Percent_Identity=41.4043583535109, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24581235, Length=485, Percent_Identity=33.1958762886598, Blast_Score=251, Evalue=8e-67,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=37.1323529411765, Blast_Score=158, Evalue=9e-39,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51981; Mature: 51981

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00110 PYRUVATE_KINASE ; PS00659 GLYCOSYL_HYDROL_F5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTKIICTIGPASEHEDVLTRMIKAGMDVARLNFSHGSHEEHQGKIDLIKKVRQKLHMP
CCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHHCCC
IAIMLDTKGPEYRIKTFENGKIELKDGDTFTLTTDDIVGNQERVSVNYENLIRDLKVGDR
EEEEEECCCCCEEEEEECCCEEEEECCCEEEEEEHHHCCCCCEEEECHHHHHHHHHCCCE
VLVANGIIILQVRELKGNDAICDVIAGGTLSDRKSMNFPNKVMKHAYLSKQDKDDLLFGI
EEEECCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEC
KNEVDYVAASFVSTKQDVADLRSFLDENGGEDIDIIAKIENRSGVDHVEEICEIANGIMI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCEEE
ARGDLGVEIPGVEVPAIQKYLINKCRMLGTRVITATEMLESMIHNPRPTRAELSDVANAV
EECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
YDGSSAIMLSGESAAGKYPVEAVKNMAETAEYTEKQINYGKRFANAEFQTKSIVDAISHA
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
TCAMAIDVDAKCIVVSSLTGRTVRMVSRFRCPVDIIGMTTSEKAWRKLNLSWGVKPVLCE
EEEEEEECCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCHHHHHEECCCCCCCEEECC
EYNSMEVMFYNAMNEAKSVMKLKKGDHIVLTGGLINGKTGNTNVIKVETV
CCCCEEEEEEEHHHHHHHHHHHCCCCEEEEECCEEECCCCCCEEEEEEEC
>Mature Secondary Structure
MRKTKIICTIGPASEHEDVLTRMIKAGMDVARLNFSHGSHEEHQGKIDLIKKVRQKLHMP
CCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHHCCC
IAIMLDTKGPEYRIKTFENGKIELKDGDTFTLTTDDIVGNQERVSVNYENLIRDLKVGDR
EEEEEECCCCCEEEEEECCCEEEEECCCEEEEEEHHHCCCCCEEEECHHHHHHHHHCCCE
VLVANGIIILQVRELKGNDAICDVIAGGTLSDRKSMNFPNKVMKHAYLSKQDKDDLLFGI
EEEECCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEC
KNEVDYVAASFVSTKQDVADLRSFLDENGGEDIDIIAKIENRSGVDHVEEICEIANGIMI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCEEE
ARGDLGVEIPGVEVPAIQKYLINKCRMLGTRVITATEMLESMIHNPRPTRAELSDVANAV
EECCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
YDGSSAIMLSGESAAGKYPVEAVKNMAETAEYTEKQINYGKRFANAEFQTKSIVDAISHA
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
TCAMAIDVDAKCIVVSSLTGRTVRMVSRFRCPVDIIGMTTSEKAWRKLNLSWGVKPVLCE
EEEEEEECCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCHHHHHEECCCCCCCEEECC
EYNSMEVMFYNAMNEAKSVMKLKKGDHIVLTGGLINGKTGNTNVIKVETV
CCCCEEEEEEEHHHHHHHHHHHCCCCEEEEECCEEECCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286; 9625784 [H]