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The map label for this gene is alaXL [H]

Identifier: 238925421

GI number: 238925421

Start: 2940097

End: 2941350

Strand: Reverse

Name: alaXL [H]

Synonym: EUBREC_3076

Alternate gene names: 238925421

Gene position: 2941350-2940097 (Counterclockwise)

Preceding gene: 238925422

Following gene: 238925418

Centisome position: 85.26

GC content: 45.61

Gene sequence:

>1254_bases
ATGAGTATGACAGAAACAATTAAACTATACGACAGGGATGCATATGCCACGGAGTTTGAGGCAGATATTATATCCTGCGA
GCCGAACAAGGCAGATGATAAGCAGTTTGATATAATATTAAATCAGACTTTATTTTTCCCGGAAGAGGGCGGACAGAGCC
CGGACATGGGAATACTTGGTGGCTACAGGGTCGTTGATGTGCAGATTAAGAATGGAGTCATTACTCATACGGTGGATATT
TCCGCAGATGATTGCTGCGAGGCAGAAAAGGAAACTGGTAAGCAAATCACCGGCAATGAATTTGAGCCGGAGAAAGTGGA
GCTTGCAGCAGGAGTCCATGTACAGGGCAAAATAGACTGGCAGCATCGTTTTTATAATATGCAGCAACACAGTGGAGAGC
ACATTTTTTCGGGAATTGTCCACCGCAGGTTTGGATTTGAGAATGTGGGCTTTCATCTGAGTGATTCTGTAGTGACAATG
GATTTCAGTGGAGTGATTTCACCGGAGGATATAGCAGAGGTGGAGCATGAGGTAAATGTAGCCATTTCAAAAAATATTCC
GATAGAGGTGACATATCCGTCAAGGGATGAGCTTGCGCAGCTGGAGTACAGAAGCAAAATCGAGATTGAAGGACAGGTCA
GAATAGTGACAGTGCCGGGGTATGACGTGTGTGCATGCTGTGCGCCGCATGTGAAGCGCACCGGCGAGATTGGCATGCTT
AAGGTTATGAATTATCAGAATTATAAAGGAGGCGTCAGAATCAGCATCCTTTGTGGTTTCAGGGCACTGGAGGCTTTCAG
ACAAAAGTGCGACATTATCTCAGAGCTAATGGGGATATTTACCACAAATCAGGAGGCTATTGTGGATAACGTCACAAAGC
TTAAGGCAGTAAACCAGTCACTTAAGTCAGAGCTTGGCACTGCAAAGTCAGCGCTTTTGGATTATAAGGTTGCAGAGCTT
CCTGCTGATACGGACAATGCAGTATTGTTTGAGGACGGCATTGATACGAATACAGCAAGAAATTGCGTCAATGGCCTTGT
GGAGAAATATAGTGGCTTTAGTGCATTTTTTACAGGAAATGATGAGGCGGGCTATAGCTTTATTATCGGAAGCAAGAATG
CAGACTGCAATACAGTTGCCGCAGCACTGAGAAATAAGCTGGGTGCCAGAGGCGGCGGTAAGCCGGTTATGGTGCAGGGC
TCTGTCAAGGCAGCAAAGAGCGAGATTGAGGAAGTGTTAAAAGAGGTGCTTTGA

Upstream 100 bases:

>100_bases
TGTGGGAAACCAGATTTAGGGGTTTCATATGAATTATGAATGCGCTATAATAGGACATAGCAATGATGCATAAGAATATA
GAGCGAGAGGAACAAGCAGT

Downstream 100 bases:

>100_bases
CTTTTTGAAAATATAGTTTGTGGGACGGATTAATAACACAAAAGCGGTTTCAGGACATTGCATCCTGAAACCGCTTTTAT
TGTAAAAATTATATCCAAAG

Product: truncated alanine-tRNA ligase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSMTETIKLYDRDAYATEFEADIISCEPNKADDKQFDIILNQTLFFPEEGGQSPDMGILGGYRVVDVQIKNGVITHTVDI
SADDCCEAEKETGKQITGNEFEPEKVELAAGVHVQGKIDWQHRFYNMQQHSGEHIFSGIVHRRFGFENVGFHLSDSVVTM
DFSGVISPEDIAEVEHEVNVAISKNIPIEVTYPSRDELAQLEYRSKIEIEGQVRIVTVPGYDVCACCAPHVKRTGEIGML
KVMNYQNYKGGVRISILCGFRALEAFRQKCDIISELMGIFTTNQEAIVDNVTKLKAVNQSLKSELGTAKSALLDYKVAEL
PADTDNAVLFEDGIDTNTARNCVNGLVEKYSGFSAFFTGNDEAGYSFIIGSKNADCNTVAAALRNKLGARGGGKPVMVQG
SVKAAKSEIEEVLKEVL

Sequences:

>Translated_417_residues
MSMTETIKLYDRDAYATEFEADIISCEPNKADDKQFDIILNQTLFFPEEGGQSPDMGILGGYRVVDVQIKNGVITHTVDI
SADDCCEAEKETGKQITGNEFEPEKVELAAGVHVQGKIDWQHRFYNMQQHSGEHIFSGIVHRRFGFENVGFHLSDSVVTM
DFSGVISPEDIAEVEHEVNVAISKNIPIEVTYPSRDELAQLEYRSKIEIEGQVRIVTVPGYDVCACCAPHVKRTGEIGML
KVMNYQNYKGGVRISILCGFRALEAFRQKCDIISELMGIFTTNQEAIVDNVTKLKAVNQSLKSELGTAKSALLDYKVAEL
PADTDNAVLFEDGIDTNTARNCVNGLVEKYSGFSAFFTGNDEAGYSFIIGSKNADCNTVAAALRNKLGARGGGKPVMVQG
SVKAAKSEIEEVLKEVL
>Mature_416_residues
SMTETIKLYDRDAYATEFEADIISCEPNKADDKQFDIILNQTLFFPEEGGQSPDMGILGGYRVVDVQIKNGVITHTVDIS
ADDCCEAEKETGKQITGNEFEPEKVELAAGVHVQGKIDWQHRFYNMQQHSGEHIFSGIVHRRFGFENVGFHLSDSVVTMD
FSGVISPEDIAEVEHEVNVAISKNIPIEVTYPSRDELAQLEYRSKIEIEGQVRIVTVPGYDVCACCAPHVKRTGEIGMLK
VMNYQNYKGGVRISILCGFRALEAFRQKCDIISELMGIFTTNQEAIVDNVTKLKAVNQSLKSELGTAKSALLDYKVAELP
ADTDNAVLFEDGIDTNTARNCVNGLVEKYSGFSAFFTGNDEAGYSFIIGSKNADCNTVAAALRNKLGARGGGKPVMVQGS
VKAAKSEIEEVLKEVL

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=412, Percent_Identity=22.0873786407767, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI217416402, Length=412, Percent_Identity=22.0873786407767, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1789048, Length=387, Percent_Identity=24.5478036175711, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324288, Length=317, Percent_Identity=23.6593059936909, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI18543305, Length=438, Percent_Identity=24.8858447488584, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 45854; Mature: 45723

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMTETIKLYDRDAYATEFEADIISCEPNKADDKQFDIILNQTLFFPEEGGQSPDMGILG
CCCCCEEEEEECCCCCEEECCEEEECCCCCCCCCEEEEEEECEEECCCCCCCCCCCCEEC
GYRVVDVQIKNGVITHTVDISADDCCEAEKETGKQITGNEFEPEKVELAAGVHVQGKIDW
CEEEEEEEECCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECEEEEEEEEH
QHRFYNMQQHSGEHIFSGIVHRRFGFENVGFHLSDSVVTMDFSGVISPEDIAEVEHEVNV
HHHHHCHHHCCCHHHHHHHHHHHCCCCCCCEEECCCEEEEEECCCCCHHHHHHHHHHEEE
AISKNIPIEVTYPSRDELAQLEYRSKIEIEGQVRIVTVPGYDVCACCAPHVKRTGEIGML
EEECCCCEEEECCCHHHHHHHHHCCEEEEECEEEEEEECCCCHHHHCCHHHHCCCCCCEE
KVMNYQNYKGGVRISILCGFRALEAFRQKCDIISELMGIFTTNQEAIVDNVTKLKAVNQS
EEECCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LKSELGTAKSALLDYKVAELPADTDNAVLFEDGIDTNTARNCVNGLVEKYSGFSAFFTGN
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECC
DEAGYSFIIGSKNADCNTVAAALRNKLGARGGGKPVMVQGSVKAAKSEIEEVLKEVL
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SMTETIKLYDRDAYATEFEADIISCEPNKADDKQFDIILNQTLFFPEEGGQSPDMGILG
CCCCEEEEEECCCCCEEECCEEEECCCCCCCCCEEEEEEECEEECCCCCCCCCCCCEEC
GYRVVDVQIKNGVITHTVDISADDCCEAEKETGKQITGNEFEPEKVELAAGVHVQGKIDW
CEEEEEEEECCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECEEEEEEEEH
QHRFYNMQQHSGEHIFSGIVHRRFGFENVGFHLSDSVVTMDFSGVISPEDIAEVEHEVNV
HHHHHCHHHCCCHHHHHHHHHHHCCCCCCCEEECCCEEEEEECCCCCHHHHHHHHHHEEE
AISKNIPIEVTYPSRDELAQLEYRSKIEIEGQVRIVTVPGYDVCACCAPHVKRTGEIGML
EEECCCCEEEECCCHHHHHHHHHCCEEEEECEEEEEEECCCCHHHHCCHHHHCCCCCCEE
KVMNYQNYKGGVRISILCGFRALEAFRQKCDIISELMGIFTTNQEAIVDNVTKLKAVNQS
EEECCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LKSELGTAKSALLDYKVAELPADTDNAVLFEDGIDTNTARNCVNGLVEKYSGFSAFFTGN
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECC
DEAGYSFIIGSKNADCNTVAAALRNKLGARGGGKPVMVQGSVKAAKSEIEEVLKEVL
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]