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The map label for this gene is rlmI [H]

Identifier: 238925418

GI number: 238925418

Start: 2935878

End: 2937185

Strand: Reverse

Name: rlmI [H]

Synonym: EUBREC_3073

Alternate gene names: 238925418

Gene position: 2937185-2935878 (Counterclockwise)

Preceding gene: 238925421

Following gene: 238925417

Centisome position: 85.14

GC content: 46.48

Gene sequence:

>1308_bases
ATGGAAAATCATACAATTGTAACACTGAAAAAAGGCGAAGGACGCACTATAAAAGCAGGCGGCGCATGGATTTTTGACAA
TGAAATAGACACAATCACAGGCACCTTTACGAACGGAGACATTGTTGTGGTGCACGATTTCGACGGATATCCGATGGGGC
GTGGCTTCATAAACCAGAATTCAAAAATCCGTATCAGAATGATGACAAGAAAAGCAGACCAACTGATTGATGACAATTTC
CTCAGGATGAGAGTGCAAAATGCCTGGGATTATCGCAAGCAGGTGATGGCAGGAGGCAATGACAATGTGTCTGCAGCAGG
TGAAACAGACACTGCAGGTGAGGCTTGTGTGGCAGGCATTGGTACAACCGGCAGACCTGATTTAAACTGCTGTCGTGTGA
TATTTGGCGAGGCTGATTTCCTGCCGGGTATTACAGTGGACAAATACGCAGATGTGCTTGTGGTGGAGTGTCTTGCACTT
GGAATGGAGCAGTTTAAGGTGAAAATCGTTGAACTGTTAAAGGAAGTGCTGGCGGCGGACGGAATAAATATTCGTGGAGT
ATATGAGCGCAGTGATGCAAATGAGCGCAAAAAAGAAGGTCTTCCTAAGGTAAAAGGCTTTATAGGAGCCGAGTTTGACA
CAAATGTGGAGATAGTAGAAAACGCAGTTCACTATATGGTCGATGTGGAAAATGGACAGAAAACAGGCTTCTTCTTAGAC
CAGAAGTATAACAGGCTTGCTATGCAGCGTATCTGCAAGGGGAAAAGAGTGCTCGACTGCTTCACGCACATGGGCACATT
TGCTCTCAATGCAGGAATTGCGGGGGCAAGTGAGGTTACAGGACTTGATATATCAGAGTATGCGGTTAAGCAGGCCACAG
AAAATGCGAAGAGAAATAATCTCTCAGACACAGTGAAGTTTCGCGTTGCAAATGTGCTGGATGAGTTGCCACGGCTCGCT
GCAGATGGTGAAAAATATGATGTAGTCATTCTGGATCCTCCGGCATTTACAAAGTCGCGTGAGGCTACCAAAAATGCGAT
AAAGGGCTACCGCGAGATTAACATGAAGGGACTTAAGCTTGTTAAGGATGGTGGATTTCTGGCTACCTGCTCATGCTCTC
ATTTCATGACGCAGGAGCTGCTTGCAAAAACGGTCAAGGAAGCGGCAAAGGCAACTCACAAGAGGCTGCGCCAGGTGGAG
TTCCGCACACAGGGACCGGATCATCCGATACTGTGGGCCAACTCAGCTAATGTACCGGAGTCGTATTATCTTAAGTTTTT
CATTTTCCAGGTCGTTGATGAGAAGTAG

Upstream 100 bases:

>100_bases
TCGACAGATAAACTTATTTCTAAAGGCTATAGAATTTCAGATAAACTATATAATCAGATTTTAGCAAGGAATAATGAAGT
AATTTAGAAGAGGAACAAAT

Downstream 100 bases:

>100_bases
ATGACAGAAAGTGACGGAAATGCTTGATTTACAAGGGCTGGAAGGTCTTTAGTGATTAGCTTGCAAACCTTGTAAAGTAT
CGCAAAATATCGTATCACAG

Product: putative methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MENHTIVTLKKGEGRTIKAGGAWIFDNEIDTITGTFTNGDIVVVHDFDGYPMGRGFINQNSKIRIRMMTRKADQLIDDNF
LRMRVQNAWDYRKQVMAGGNDNVSAAGETDTAGEACVAGIGTTGRPDLNCCRVIFGEADFLPGITVDKYADVLVVECLAL
GMEQFKVKIVELLKEVLAADGINIRGVYERSDANERKKEGLPKVKGFIGAEFDTNVEIVENAVHYMVDVENGQKTGFFLD
QKYNRLAMQRICKGKRVLDCFTHMGTFALNAGIAGASEVTGLDISEYAVKQATENAKRNNLSDTVKFRVANVLDELPRLA
ADGEKYDVVILDPPAFTKSREATKNAIKGYREINMKGLKLVKDGGFLATCSCSHFMTQELLAKTVKEAAKATHKRLRQVE
FRTQGPDHPILWANSANVPESYYLKFFIFQVVDEK

Sequences:

>Translated_435_residues
MENHTIVTLKKGEGRTIKAGGAWIFDNEIDTITGTFTNGDIVVVHDFDGYPMGRGFINQNSKIRIRMMTRKADQLIDDNF
LRMRVQNAWDYRKQVMAGGNDNVSAAGETDTAGEACVAGIGTTGRPDLNCCRVIFGEADFLPGITVDKYADVLVVECLAL
GMEQFKVKIVELLKEVLAADGINIRGVYERSDANERKKEGLPKVKGFIGAEFDTNVEIVENAVHYMVDVENGQKTGFFLD
QKYNRLAMQRICKGKRVLDCFTHMGTFALNAGIAGASEVTGLDISEYAVKQATENAKRNNLSDTVKFRVANVLDELPRLA
ADGEKYDVVILDPPAFTKSREATKNAIKGYREINMKGLKLVKDGGFLATCSCSHFMTQELLAKTVKEAAKATHKRLRQVE
FRTQGPDHPILWANSANVPESYYLKFFIFQVVDEK
>Mature_435_residues
MENHTIVTLKKGEGRTIKAGGAWIFDNEIDTITGTFTNGDIVVVHDFDGYPMGRGFINQNSKIRIRMMTRKADQLIDDNF
LRMRVQNAWDYRKQVMAGGNDNVSAAGETDTAGEACVAGIGTTGRPDLNCCRVIFGEADFLPGITVDKYADVLVVECLAL
GMEQFKVKIVELLKEVLAADGINIRGVYERSDANERKKEGLPKVKGFIGAEFDTNVEIVENAVHYMVDVENGQKTGFFLD
QKYNRLAMQRICKGKRVLDCFTHMGTFALNAGIAGASEVTGLDISEYAVKQATENAKRNNLSDTVKFRVANVLDELPRLA
ADGEKYDVVILDPPAFTKSREATKNAIKGYREINMKGLKLVKDGGFLATCSCSHFMTQELLAKTVKEAAKATHKRLRQVE
FRTQGPDHPILWANSANVPESYYLKFFIFQVVDEK

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=428, Percent_Identity=31.0747663551402, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1787181, Length=284, Percent_Identity=27.112676056338, Blast_Score=82, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 48338; Mature: 48338

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENHTIVTLKKGEGRTIKAGGAWIFDNEIDTITGTFTNGDIVVVHDFDGYPMGRGFINQN
CCCCEEEEEECCCCCEEECCCEEEECCCCCEEEEEEECCCEEEEECCCCCCCCCCEECCC
SKIRIRMMTRKADQLIDDNFLRMRVQNAWDYRKQVMAGGNDNVSAAGETDTAGEACVAGI
CEEEEEEEHHHHHHHHCCCHHEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHEECC
GTTGRPDLNCCRVIFGEADFLPGITVDKYADVLVVECLALGMEQFKVKIVELLKEVLAAD
CCCCCCCCHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
GINIRGVYERSDANERKKEGLPKVKGFIGAEFDTNVEIVENAVHYMVDVENGQKTGFFLD
CCCEEEEEECCCCCHHHHCCCCHHHHHCCCCCCCCHHHHHHHEEEEEECCCCCCCCEEHH
QKYNRLAMQRICKGKRVLDCFTHMGTFALNAGIAGASEVTGLDISEYAVKQATENAKRNN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LSDTVKFRVANVLDELPRLAADGEKYDVVILDPPAFTKSREATKNAIKGYREINMKGLKL
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEE
VKDGGFLATCSCSHFMTQELLAKTVKEAAKATHKRLRQVEFRTQGPDHPILWANSANVPE
EECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCH
SYYLKFFIFQVVDEK
HHHHHHHEEHHHCCC
>Mature Secondary Structure
MENHTIVTLKKGEGRTIKAGGAWIFDNEIDTITGTFTNGDIVVVHDFDGYPMGRGFINQN
CCCCEEEEEECCCCCEEECCCEEEECCCCCEEEEEEECCCEEEEECCCCCCCCCCEECCC
SKIRIRMMTRKADQLIDDNFLRMRVQNAWDYRKQVMAGGNDNVSAAGETDTAGEACVAGI
CEEEEEEEHHHHHHHHCCCHHEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHEECC
GTTGRPDLNCCRVIFGEADFLPGITVDKYADVLVVECLALGMEQFKVKIVELLKEVLAAD
CCCCCCCCHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
GINIRGVYERSDANERKKEGLPKVKGFIGAEFDTNVEIVENAVHYMVDVENGQKTGFFLD
CCCEEEEEECCCCCHHHHCCCCHHHHHCCCCCCCCHHHHHHHEEEEEECCCCCCCCEEHH
QKYNRLAMQRICKGKRVLDCFTHMGTFALNAGIAGASEVTGLDISEYAVKQATENAKRNN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
LSDTVKFRVANVLDELPRLAADGEKYDVVILDPPAFTKSREATKNAIKGYREINMKGLKL
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEE
VKDGGFLATCSCSHFMTQELLAKTVKEAAKATHKRLRQVEFRTQGPDHPILWANSANVPE
EECCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCH
SYYLKFFIFQVVDEK
HHHHHHHEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA