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The map label for this gene is dacB [H]

Identifier: 238925374

GI number: 238925374

Start: 2903913

End: 2905016

Strand: Reverse

Name: dacB [H]

Synonym: EUBREC_3029

Alternate gene names: 238925374

Gene position: 2905016-2903913 (Counterclockwise)

Preceding gene: 238925375

Following gene: 238925373

Centisome position: 84.21

GC content: 44.66

Gene sequence:

>1104_bases
ATGAGAAATAATAGCAGAAAAAATTACAGACGCCGCAGACAAAATACAAGAGCTTCACAGATTAAGCTTGTAGGAATAGT
GGCTGTGATAGTCGTTATAGCGATTATAATAGTTATATCGTGCTTTAATAAAACAAAAAAAGACAGTAATAAGGCAAAAG
ACACAGGAACGGCCACAGAAAATGTGACCGGTGTGACTGAGCAGGTAAGTGAGACAGAGGAAGCAAAGGAGCTCTTTGCA
CCAAAGGAGTCTGATGCTACAGCAGCAATCGGAGATGAGCTTGAGAGCTCTTATGCCGTAGTGATTGATGAGCAGGACGG
CACAGTTGTTGCGGGAAAAAATGCTCATGAGAAAATGTATCCGGCTTCCATGACAAAGGTTATGACAGTGCTTGTAGCAG
CAGAGCAGGCTGAGAAAAATGCAATTGCTGCAGCGGAGAATGAAAACACCGAAATAGACAGGCAGGCTGTGATAGAAAAC
AAGCTCAACGAGGTTGTTACAATTACAAGAGAAGCCACAGATTATTCATATGTGCATGATTGCAGTACAACAGGCTTTTT
AGACGGTGAGCAGGTGCCTCTTCGCGATGTGTTTAACGGAACCATCCTGCCATCAGGTGGAGAATCGGCATATCAGCTTG
CGGTTTATACAGCAGGCTCCATGGAAGCATTTGTCGATATGATGAACCAGAAGGCAGCAGAGCTTGGTATAGGCCAGACC
ACACATTTTGCAAACCCTGTCGGCATATATGATGAGAACAATTACAGCACAGCCTATGATATGGCGGTCATCATGCAGGC
AGCGATGCAGGACGACATTTGTAAAACAGTACTTTCAAATCATACATATAGCACGGCTGCAACACCGCAGCATCCGGAGA
ACCTGAACATATCAAATCTGTTCCTGCGCCGAATAGAGGACAGGATGACAAGTGGCGAGGTAATCGGTGCAAAGACAGGC
TTTGTAAATCAGTCCGGCAACTGTGCGGTCAGCTATGAGAAGGCAGCAAGCGGAAAGACATATATATGTGTGACAGCAGG
CGCTTCAAGCGGATGGCACTGTATAGATAATCATGTGTATTTATATAATACATACGGCAAGTAA

Upstream 100 bases:

>100_bases
ATACTAAATAGGTTACAGTATAAAAATGAAATGTGATTATTCAAGTGTGGTTTTGCGAATGTGCGTGGGATGAATAGTTA
CAACGAGACAGGGATTGGAT

Downstream 100 bases:

>100_bases
TCAGCACAGCTGATTTTAAGGAGCGCAGATATGGCGGGAAATCAAGGCAATTACAGGGAAAACCCCACGCGTAATGAGAA
GAAATACAAAAAAGCCAACG

Product: putative D-alanyl-D-alanine carboxypeptidase precursor

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MRNNSRKNYRRRRQNTRASQIKLVGIVAVIVVIAIIIVISCFNKTKKDSNKAKDTGTATENVTGVTEQVSETEEAKELFA
PKESDATAAIGDELESSYAVVIDEQDGTVVAGKNAHEKMYPASMTKVMTVLVAAEQAEKNAIAAAENENTEIDRQAVIEN
KLNEVVTITREATDYSYVHDCSTTGFLDGEQVPLRDVFNGTILPSGGESAYQLAVYTAGSMEAFVDMMNQKAAELGIGQT
THFANPVGIYDENNYSTAYDMAVIMQAAMQDDICKTVLSNHTYSTAATPQHPENLNISNLFLRRIEDRMTSGEVIGAKTG
FVNQSGNCAVSYEKAASGKTYICVTAGASSGWHCIDNHVYLYNTYGK

Sequences:

>Translated_367_residues
MRNNSRKNYRRRRQNTRASQIKLVGIVAVIVVIAIIIVISCFNKTKKDSNKAKDTGTATENVTGVTEQVSETEEAKELFA
PKESDATAAIGDELESSYAVVIDEQDGTVVAGKNAHEKMYPASMTKVMTVLVAAEQAEKNAIAAAENENTEIDRQAVIEN
KLNEVVTITREATDYSYVHDCSTTGFLDGEQVPLRDVFNGTILPSGGESAYQLAVYTAGSMEAFVDMMNQKAAELGIGQT
THFANPVGIYDENNYSTAYDMAVIMQAAMQDDICKTVLSNHTYSTAATPQHPENLNISNLFLRRIEDRMTSGEVIGAKTG
FVNQSGNCAVSYEKAASGKTYICVTAGASSGWHCIDNHVYLYNTYGK
>Mature_367_residues
MRNNSRKNYRRRRQNTRASQIKLVGIVAVIVVIAIIIVISCFNKTKKDSNKAKDTGTATENVTGVTEQVSETEEAKELFA
PKESDATAAIGDELESSYAVVIDEQDGTVVAGKNAHEKMYPASMTKVMTVLVAAEQAEKNAIAAAENENTEIDRQAVIEN
KLNEVVTITREATDYSYVHDCSTTGFLDGEQVPLRDVFNGTILPSGGESAYQLAVYTAGSMEAFVDMMNQKAAELGIGQT
THFANPVGIYDENNYSTAYDMAVIMQAAMQDDICKTVLSNHTYSTAATPQHPENLNISNLFLRRIEDRMTSGEVIGAKTG
FVNQSGNCAVSYEKAASGKTYICVTAGASSGWHCIDNHVYLYNTYGK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=197, Percent_Identity=31.4720812182741, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786851, Length=181, Percent_Identity=32.5966850828729, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082022, Length=180, Percent_Identity=30, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 39944; Mature: 39944

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNNSRKNYRRRRQNTRASQIKLVGIVAVIVVIAIIIVISCFNKTKKDSNKAKDTGTATE
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
NVTGVTEQVSETEEAKELFAPKESDATAAIGDELESSYAVVIDEQDGTVVAGKNAHEKMY
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEEECCCCEEEECCCCCHHCC
PASMTKVMTVLVAAEQAEKNAIAAAENENTEIDRQAVIENKLNEVVTITREATDYSYVHD
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCEEEC
CSTTGFLDGEQVPLRDVFNGTILPSGGESAYQLAVYTAGSMEAFVDMMNQKAAELGIGQT
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
THFANPVGIYDENNYSTAYDMAVIMQAAMQDDICKTVLSNHTYSTAATPQHPENLNISNL
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
FLRRIEDRMTSGEVIGAKTGFVNQSGNCAVSYEKAASGKTYICVTAGASSGWHCIDNHVY
HHHHHHHHCCCCCEEECCCCCCCCCCCEEEEEHHCCCCCEEEEEEECCCCCCEEECCCEE
LYNTYGK
EEECCCC
>Mature Secondary Structure
MRNNSRKNYRRRRQNTRASQIKLVGIVAVIVVIAIIIVISCFNKTKKDSNKAKDTGTATE
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
NVTGVTEQVSETEEAKELFAPKESDATAAIGDELESSYAVVIDEQDGTVVAGKNAHEKMY
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEEEECCCCEEEECCCCCHHCC
PASMTKVMTVLVAAEQAEKNAIAAAENENTEIDRQAVIENKLNEVVTITREATDYSYVHD
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCEEEC
CSTTGFLDGEQVPLRDVFNGTILPSGGESAYQLAVYTAGSMEAFVDMMNQKAAELGIGQT
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
THFANPVGIYDENNYSTAYDMAVIMQAAMQDDICKTVLSNHTYSTAATPQHPENLNISNL
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
FLRRIEDRMTSGEVIGAKTGFVNQSGNCAVSYEKAASGKTYICVTAGASSGWHCIDNHVY
HHHHHHHHCCCCCEEECCCCCCCCCCCEEEEEHHCCCCCEEEEEEECCCCCCEEECCCEE
LYNTYGK
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]