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The map label for this gene is truA [H]

Identifier: 238925343

GI number: 238925343

Start: 2876989

End: 2877747

Strand: Reverse

Name: truA [H]

Synonym: EUBREC_2998

Alternate gene names: 238925343

Gene position: 2877747-2876989 (Counterclockwise)

Preceding gene: 238925344

Following gene: 238925342

Centisome position: 83.42

GC content: 47.43

Gene sequence:

>759_bases
GTGCAGGCAAAACAGGAGAGATATATGCGTGTAAAAATGATTGTAGCCTACGATGGCACAAATTACTGCGGCTGGCAGAT
ACAGCCAAACGGCATAACAATTGAGCAGGTATTAAACGAGCACCTGAGCCGCCTGTTAAAGGAGGATATAAAGGTGACAG
GAGCAAGCCGCACCGATGCGGGAGTGCATTCACTTGGAAATGTGTGTATGTTTGACACCAATACCCGCATGCCGGCGGAA
AAGATATCGTATGCACTGAATCAGAAGCTGCCGGAGGATATAGTGGTGGTCGATTCCTGTGAGGTAAGCGATGATTTTCA
TCCACGCTTCTCAAAGAGCCGTAAAACCTATGAATACCGCATATTAAATGCGAGGTTCAGAAATCCGACACGCAGGCTTG
ACACATATTTCTATCATTATCCGCTTGACACAGAAAAGATGAGTGAGGCTGCAAAATATCTTATAGGAGAGCATGATTTT
GCAAGCTTCTGCTCGGCAAACTCGCAGGCACAGACCACAGTCCGCACGATATATGACTGTACGGTGTCAAAGGCCGGTGA
CATCATCACAATACGTGTGACAGGAAACGGCTTTTTATACAATATGGTGCGCATAATCGCAGGAACACTTGTGAAGATAG
GTGGAAGCGATATGCCGGCCGATGCGATGAAGGACATAATAGCCACAACAGACAGGGCAGCAGCAGGTCCTACAGCCCCG
GCACATGGGCTTACAATGATAGGACTCAAGTACGAGTAA

Upstream 100 bases:

>100_bases
CATATCTTTTCATATTTGCTTTTTTAGCGGTTGTGATAGTGCTTAGAATCCTTCTGCCGGTGAATCTGGGCAATCTTATT
TTCAGATAGTGAAATAGATA

Downstream 100 bases:

>100_bases
CAGCTTTAGATTTTTAATATTATATTAAGATTTTTGGAATATCAGTTTTTAGCTTCAATAATAGGCTATAATGTTGTTAC
TGTTAATACACGGTACGGTA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MQAKQERYMRVKMIVAYDGTNYCGWQIQPNGITIEQVLNEHLSRLLKEDIKVTGASRTDAGVHSLGNVCMFDTNTRMPAE
KISYALNQKLPEDIVVVDSCEVSDDFHPRFSKSRKTYEYRILNARFRNPTRRLDTYFYHYPLDTEKMSEAAKYLIGEHDF
ASFCSANSQAQTTVRTIYDCTVSKAGDIITIRVTGNGFLYNMVRIIAGTLVKIGGSDMPADAMKDIIATTDRAAAGPTAP
AHGLTMIGLKYE

Sequences:

>Translated_252_residues
MQAKQERYMRVKMIVAYDGTNYCGWQIQPNGITIEQVLNEHLSRLLKEDIKVTGASRTDAGVHSLGNVCMFDTNTRMPAE
KISYALNQKLPEDIVVVDSCEVSDDFHPRFSKSRKTYEYRILNARFRNPTRRLDTYFYHYPLDTEKMSEAAKYLIGEHDF
ASFCSANSQAQTTVRTIYDCTVSKAGDIITIRVTGNGFLYNMVRIIAGTLVKIGGSDMPADAMKDIIATTDRAAAGPTAP
AHGLTMIGLKYE
>Mature_252_residues
MQAKQERYMRVKMIVAYDGTNYCGWQIQPNGITIEQVLNEHLSRLLKEDIKVTGASRTDAGVHSLGNVCMFDTNTRMPAE
KISYALNQKLPEDIVVVDSCEVSDDFHPRFSKSRKTYEYRILNARFRNPTRRLDTYFYHYPLDTEKMSEAAKYLIGEHDF
ASFCSANSQAQTTVRTIYDCTVSKAGDIITIRVTGNGFLYNMVRIIAGTLVKIGGSDMPADAMKDIIATTDRAAAGPTAP
AHGLTMIGLKYE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=265, Percent_Identity=31.6981132075472, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI199559785, Length=275, Percent_Identity=26.1818181818182, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788657, Length=248, Percent_Identity=34.6774193548387, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71985046, Length=241, Percent_Identity=26.1410788381743, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321112, Length=250, Percent_Identity=27.6, Blast_Score=75, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281362146, Length=259, Percent_Identity=25.8687258687259, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648455, Length=259, Percent_Identity=25.8687258687259, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28261; Mature: 28261

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAKQERYMRVKMIVAYDGTNYCGWQIQPNGITIEQVLNEHLSRLLKEDIKVTGASRTDA
CCCHHHHCEEEEEEEEECCCCCCCEEEECCCEEHHHHHHHHHHHHHHHHHEECCCCCCCH
GVHSLGNVCMFDTNTRMPAEKISYALNQKLPEDIVVVDSCEVSDDFHPRFSKSRKTYEYR
HHHHHCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHCCCCEEEEE
ILNARFRNPTRRLDTYFYHYPLDTEKMSEAAKYLIGEHDFASFCSANSQAQTTVRTIYDC
EEECHHCCHHHHHCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHH
TVSKAGDIITIRVTGNGFLYNMVRIIAGTLVKIGGSDMPADAMKDIIATTDRAAAGPTAP
HCCCCCCEEEEEECCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCCCCCCCCC
AHGLTMIGLKYE
CCCEEEEEEECC
>Mature Secondary Structure
MQAKQERYMRVKMIVAYDGTNYCGWQIQPNGITIEQVLNEHLSRLLKEDIKVTGASRTDA
CCCHHHHCEEEEEEEEECCCCCCCEEEECCCEEHHHHHHHHHHHHHHHHHEECCCCCCCH
GVHSLGNVCMFDTNTRMPAEKISYALNQKLPEDIVVVDSCEVSDDFHPRFSKSRKTYEYR
HHHHHCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHCCCCEEEEE
ILNARFRNPTRRLDTYFYHYPLDTEKMSEAAKYLIGEHDFASFCSANSQAQTTVRTIYDC
EEECHHCCHHHHHCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHH
TVSKAGDIITIRVTGNGFLYNMVRIIAGTLVKIGGSDMPADAMKDIIATTDRAAAGPTAP
HCCCCCCEEEEEECCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCCCCCCCCC
AHGLTMIGLKYE
CCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA