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The map label for this gene is glgX [H]

Identifier: 238925309

GI number: 238925309

Start: 2847043

End: 2849175

Strand: Reverse

Name: glgX [H]

Synonym: EUBREC_2964

Alternate gene names: 238925309

Gene position: 2849175-2847043 (Counterclockwise)

Preceding gene: 238925310

Following gene: 238925306

Centisome position: 82.59

GC content: 47.44

Gene sequence:

>2133_bases
ATGTATATGTGGAGAGAGCGCAGTAAAGAAGAAAAGCATGAGGATGTAGTAAATACAGGCCTGCTGCCGCTTGATGTGGT
AGAGGGATTTAAGATACGTCCGGGCTTTTTCAGAATGTACGGAGCGTGCGTGGCATCGAACGGAGTGAGCTTTACCATCA
ATTCCCATGGAGCAACAAGGTGTACTCTTTTATTGTTCAAGCCACAGGCACCAAAACCGTATGCGAGGATTCCGTTTCCT
GATTCATATAGGATAGGAGATACCTACTCCATGCTTGTTTTTGATATAAAGCCGGATGAATTTGAGTATGCATTTTCGTT
TGACGGACCATATGAGCCGGCAAAGGGACTTTTGTTTAACGAGGAAAATGTCCTTTTAGACCCATATTCCAGAGCGGTCA
CTGGACAGCGAAAATGGGGAGAAAAGCCGGAGGGTGGCAAGGATTTTGAGTACAGAGCCAGAGTTGTAAAAAGCAGCTTT
GACTGGGGCAACATAAAGCAGCTTGAGCAACCCTTTGAGGACCTTGTGATTTATGAGACACATGTGAGGGGCTATACAAA
GGATAAGTCATCTGGAGTCAGCGCACCGGGTACATTTGCCGGTTTGAAGGATAAGATTCCGTATCTTAAGGACCTTGGAA
TAAATGCAGTGGAGCTCATGCCGATTTTTGAGTTCGATGAGATGGAGAGCGCGAGAGTGGTCGATGGTGTGCAGCTTTAC
AATTACTGGGGCTACAACACAGTATCATTTTTTGCACCGAATACCAGCTATGCCTTCAACGAGGAGCACAACCACGAGGG
AGACGAGCTGAAATCGCTGATAAAGGCTTTAAAGGAAAATGGCATAGAGGTCATTCTTGATGTGGTGTTCAACCATACCG
CTGAGGGAAATGAGATGGGACCGTGCTTCTCCTTCAAGGGAATAGACAATAACGTGTACTATATGCTTACACCGGATGCA
CACTACTACAATTTCAGTGGCTGTGGAAACGTTATGAACTGTAATCATCCTGTTGTGCGCAGCTTTATCATAGACTGTCT
GAGACACTGGGCCATTGAGTACAGAGTGGATGGCTTCAGATTTGACCTGGCTTCCATTCTCGGAAGAGACCAAAACGGTG
CACCTATGGCCAATCCGCCGATTCTTGAGTCGCTGGCATTCGACCCGGTACTTGGCAAGATGAAGCTCATCGCAGAGGCC
TGGGATGCAGGCGGACTGTATCAGGTGGGAAGCTTCCCGTCATGGAACAGATGGGCTGAGTGGAACGGCAGATACAGGGA
TGACATGAGAAGCTTTTTAAAGGGCGATGACGGTATGGCGGGAAATGCCATCACACGTATCACCGGCTCACGCGACCTTT
ACAGCCCGGAGAGCAGAGGACACAAGGCATCAGTCAATTTCCTGACCTGTCATGATGGCTTTACACTGTATGACCTTTAC
TCATACAATGAAAAGCACAATGAGAAAAACGGCTGGAATAACACAGACGGAGACAACAACGGACATAGCTGGAACTGTGG
CGCAGAGGGCGAGACAGACGATCCAAATGTAAACGGACTTAGAAGGCGCCTTATAAAGAATGCATTTGCAGCGCTTTTGT
GCAGCCGTGGCCCGGCGATGTTTTTTGCGGGCGATGAGTTCTGTAACACCCAGTTTGGAAACAACAACGCGTATTGTCAG
GACAATATCATCTCATGGCTCGACTGGAGCAGGCTTGAGGAATTCAAGGAAATCCATGATTTCGTGCGTCATATGATTCA
GTTCAGGAAAGAGCATCCTATACTCAGAAAGATGACAAAGCCTTCATCCTGCCAGTTCCCGGAAATCAGTGTGCACAACG
GTACACCGTTCAATGCCTCAACGGATTACAAGACAAAGCTTATCGGCATCATGTATGCCGGAAGAAATGAGGAGGACACG
GAGGATGATATTGTATTCTATTGCATGAATGCATATTGGGAGCCGCTTGTAATGCAGCTTCCGGTGCTTCCGAACGGAAA
ACACTGGCATGTGGACACCAACACCAATGCAGAGTATTTTGACGGAGAGGACTTCACGGCAAAGACAGAGCTTTTGGGTG
TCAACACCATAAGAGTTCCTGCAAGGACCACGATAATACTTGTGGCAGAATAA

Upstream 100 bases:

>100_bases
ATAGTTACAATATCATTTTTTTACGAATTAAGATAAAAAGCAGTCATGCAACAAAAGGCATGTACCATCATACAAATATA
GTAAGGGAGCAAAGAGCAAT

Downstream 100 bases:

>100_bases
CAAAAAAACAGCCCGGAACACATTGTGATATGTGCACCGGGCTGTTTTTTGTTACGGTTTTGGCTTAGACTGTAACATTT
CCCCCTTTCATTTTTCAATA

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 710; Mature: 710

Protein sequence:

>710_residues
MYMWRERSKEEKHEDVVNTGLLPLDVVEGFKIRPGFFRMYGACVASNGVSFTINSHGATRCTLLLFKPQAPKPYARIPFP
DSYRIGDTYSMLVFDIKPDEFEYAFSFDGPYEPAKGLLFNEENVLLDPYSRAVTGQRKWGEKPEGGKDFEYRARVVKSSF
DWGNIKQLEQPFEDLVIYETHVRGYTKDKSSGVSAPGTFAGLKDKIPYLKDLGINAVELMPIFEFDEMESARVVDGVQLY
NYWGYNTVSFFAPNTSYAFNEEHNHEGDELKSLIKALKENGIEVILDVVFNHTAEGNEMGPCFSFKGIDNNVYYMLTPDA
HYYNFSGCGNVMNCNHPVVRSFIIDCLRHWAIEYRVDGFRFDLASILGRDQNGAPMANPPILESLAFDPVLGKMKLIAEA
WDAGGLYQVGSFPSWNRWAEWNGRYRDDMRSFLKGDDGMAGNAITRITGSRDLYSPESRGHKASVNFLTCHDGFTLYDLY
SYNEKHNEKNGWNNTDGDNNGHSWNCGAEGETDDPNVNGLRRRLIKNAFAALLCSRGPAMFFAGDEFCNTQFGNNNAYCQ
DNIISWLDWSRLEEFKEIHDFVRHMIQFRKEHPILRKMTKPSSCQFPEISVHNGTPFNASTDYKTKLIGIMYAGRNEEDT
EDDIVFYCMNAYWEPLVMQLPVLPNGKHWHVDTNTNAEYFDGEDFTAKTELLGVNTIRVPARTTIILVAE

Sequences:

>Translated_710_residues
MYMWRERSKEEKHEDVVNTGLLPLDVVEGFKIRPGFFRMYGACVASNGVSFTINSHGATRCTLLLFKPQAPKPYARIPFP
DSYRIGDTYSMLVFDIKPDEFEYAFSFDGPYEPAKGLLFNEENVLLDPYSRAVTGQRKWGEKPEGGKDFEYRARVVKSSF
DWGNIKQLEQPFEDLVIYETHVRGYTKDKSSGVSAPGTFAGLKDKIPYLKDLGINAVELMPIFEFDEMESARVVDGVQLY
NYWGYNTVSFFAPNTSYAFNEEHNHEGDELKSLIKALKENGIEVILDVVFNHTAEGNEMGPCFSFKGIDNNVYYMLTPDA
HYYNFSGCGNVMNCNHPVVRSFIIDCLRHWAIEYRVDGFRFDLASILGRDQNGAPMANPPILESLAFDPVLGKMKLIAEA
WDAGGLYQVGSFPSWNRWAEWNGRYRDDMRSFLKGDDGMAGNAITRITGSRDLYSPESRGHKASVNFLTCHDGFTLYDLY
SYNEKHNEKNGWNNTDGDNNGHSWNCGAEGETDDPNVNGLRRRLIKNAFAALLCSRGPAMFFAGDEFCNTQFGNNNAYCQ
DNIISWLDWSRLEEFKEIHDFVRHMIQFRKEHPILRKMTKPSSCQFPEISVHNGTPFNASTDYKTKLIGIMYAGRNEEDT
EDDIVFYCMNAYWEPLVMQLPVLPNGKHWHVDTNTNAEYFDGEDFTAKTELLGVNTIRVPARTTIILVAE
>Mature_710_residues
MYMWRERSKEEKHEDVVNTGLLPLDVVEGFKIRPGFFRMYGACVASNGVSFTINSHGATRCTLLLFKPQAPKPYARIPFP
DSYRIGDTYSMLVFDIKPDEFEYAFSFDGPYEPAKGLLFNEENVLLDPYSRAVTGQRKWGEKPEGGKDFEYRARVVKSSF
DWGNIKQLEQPFEDLVIYETHVRGYTKDKSSGVSAPGTFAGLKDKIPYLKDLGINAVELMPIFEFDEMESARVVDGVQLY
NYWGYNTVSFFAPNTSYAFNEEHNHEGDELKSLIKALKENGIEVILDVVFNHTAEGNEMGPCFSFKGIDNNVYYMLTPDA
HYYNFSGCGNVMNCNHPVVRSFIIDCLRHWAIEYRVDGFRFDLASILGRDQNGAPMANPPILESLAFDPVLGKMKLIAEA
WDAGGLYQVGSFPSWNRWAEWNGRYRDDMRSFLKGDDGMAGNAITRITGSRDLYSPESRGHKASVNFLTCHDGFTLYDLY
SYNEKHNEKNGWNNTDGDNNGHSWNCGAEGETDDPNVNGLRRRLIKNAFAALLCSRGPAMFFAGDEFCNTQFGNNNAYCQ
DNIISWLDWSRLEEFKEIHDFVRHMIQFRKEHPILRKMTKPSSCQFPEISVHNGTPFNASTDYKTKLIGIMYAGRNEEDT
EDDIVFYCMNAYWEPLVMQLPVLPNGKHWHVDTNTNAEYFDGEDFTAKTELLGVNTIRVPARTTIILVAE

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=570, Percent_Identity=39.2982456140351, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 80966; Mature: 80966

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMWRERSKEEKHEDVVNTGLLPLDVVEGFKIRPGFFRMYGACVASNGVSFTINSHGATR
CCCCCCCCHHHHHHHHHHCCCCCHHHHCCCEECCHHHHHHHHHHCCCCEEEEEECCCCCE
CTLLLFKPQAPKPYARIPFPDSYRIGDTYSMLVFDIKPDEFEYAFSFDGPYEPAKGLLFN
EEEEEECCCCCCCCEECCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCEEC
EENVLLDPYSRAVTGQRKWGEKPEGGKDFEYRARVVKSSFDWGNIKQLEQPFEDLVIYET
CCCEEECCHHHHHCCCHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHCCHHHEEEEEE
HVRGYTKDKSSGVSAPGTFAGLKDKIPYLKDLGINAVELMPIFEFDEMESARVVDGVQLY
CCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCCEEEEEEEECCCCCCCCHHHCCEEEE
NYWGYNTVSFFAPNTSYAFNEEHNHEGDELKSLIKALKENGIEVILDVVFNHTAEGNEMG
EECCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCC
PCFSFKGIDNNVYYMLTPDAHYYNFSGCGNVMNCNHPVVRSFIIDCLRHWAIEYRVDGFR
CEEEEECCCCCEEEEECCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCEE
FDLASILGRDQNGAPMANPPILESLAFDPVLGKMKLIAEAWDAGGLYQVGSFPSWNRWAE
EEHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
WNGRYRDDMRSFLKGDDGMAGNAITRITGSRDLYSPESRGHKASVNFLTCHDGFTLYDLY
CCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCEEEEEE
SYNEKHNEKNGWNNTDGDNNGHSWNCGAEGETDDPNVNGLRRRLIKNAFAALLCSRGPAM
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
FFAGDEFCNTQFGNNNAYCQDNIISWLDWSRLEEFKEIHDFVRHMIQFRKEHPILRKMTK
EEECCHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
PSSCQFPEISVHNGTPFNASTDYKTKLIGIMYAGRNEEDTEDDIVFYCMNAYWEPLVMQL
CCCCCCCEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEHHHCCCCEEEEE
PVLPNGKHWHVDTNTNAEYFDGEDFTAKTELLGVNTIRVPARTTIILVAE
CCCCCCCEEEEECCCCCCEECCCCCEEEEEEEECEEEEECCCEEEEEEEC
>Mature Secondary Structure
MYMWRERSKEEKHEDVVNTGLLPLDVVEGFKIRPGFFRMYGACVASNGVSFTINSHGATR
CCCCCCCCHHHHHHHHHHCCCCCHHHHCCCEECCHHHHHHHHHHCCCCEEEEEECCCCCE
CTLLLFKPQAPKPYARIPFPDSYRIGDTYSMLVFDIKPDEFEYAFSFDGPYEPAKGLLFN
EEEEEECCCCCCCCEECCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCEEC
EENVLLDPYSRAVTGQRKWGEKPEGGKDFEYRARVVKSSFDWGNIKQLEQPFEDLVIYET
CCCEEECCHHHHHCCCHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHCCHHHEEEEEE
HVRGYTKDKSSGVSAPGTFAGLKDKIPYLKDLGINAVELMPIFEFDEMESARVVDGVQLY
CCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHCCCCEEEEEEEECCCCCCCCHHHCCEEEE
NYWGYNTVSFFAPNTSYAFNEEHNHEGDELKSLIKALKENGIEVILDVVFNHTAEGNEMG
EECCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCC
PCFSFKGIDNNVYYMLTPDAHYYNFSGCGNVMNCNHPVVRSFIIDCLRHWAIEYRVDGFR
CEEEEECCCCCEEEEECCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCEE
FDLASILGRDQNGAPMANPPILESLAFDPVLGKMKLIAEAWDAGGLYQVGSFPSWNRWAE
EEHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCC
WNGRYRDDMRSFLKGDDGMAGNAITRITGSRDLYSPESRGHKASVNFLTCHDGFTLYDLY
CCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCEEEEEE
SYNEKHNEKNGWNNTDGDNNGHSWNCGAEGETDDPNVNGLRRRLIKNAFAALLCSRGPAM
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
FFAGDEFCNTQFGNNNAYCQDNIISWLDWSRLEEFKEIHDFVRHMIQFRKEHPILRKMTK
EEECCHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
PSSCQFPEISVHNGTPFNASTDYKTKLIGIMYAGRNEEDTEDDIVFYCMNAYWEPLVMQL
CCCCCCCEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEEHHHCCCCEEEEE
PVLPNGKHWHVDTNTNAEYFDGEDFTAKTELLGVNTIRVPARTTIILVAE
CCCCCCCEEEEECCCCCCEECCCCCEEEEEEEECEEEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]