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The map label for this gene is rfbX [H]

Identifier: 238924979

GI number: 238924979

Start: 2509390

End: 2510805

Strand: Direct

Name: rfbX [H]

Synonym: EUBREC_2630

Alternate gene names: 238924979

Gene position: 2509390-2510805 (Clockwise)

Preceding gene: 238924970

Following gene: 238924983

Centisome position: 72.74

GC content: 32.84

Gene sequence:

>1416_bases
ATGAATATAATACTTACGCTCTCATCCATTATCTTTCCACTCATAACTTTCCCTTATGTATCTAGAGTTTTGCAGCCTGA
AGCCTGGGGTAAAGTCAACCTTGCAACATCATTTATAACCTATTTCAGTTATTTTGCCCAGTTAGGTCTGCCTACATACG
GAGTAAAAATATGCGCCAAATACAGAGATGACAGGCTAAAACTATCACGTACCGTACAGGAACTGTCAATTATCTGTACC
ATTTCCACCATAATAACGTATGTAATTCTTGGAATAACAATTATTACCATTCCTAAGATAGCATCTGAAAAGCCATTATA
TATACTCGTTTCATCCACTCTTTTCTTCAATCTGATTGGAATGGAATGGCTATACAAAGCATTGGAAAAATACACGTACA
TTACGATAAGATCTCTTATTTTCAAATTTATTGCCTTAATAGGAATGTTTTTATTGGTAAAAAGTCAATCAGATTATATA
ATCTATGGTGGAATAAGTGTATTTGCTGCATCTGCATCAAATATCTGTAATTTTATCGAAGCACGAAAATATATAACTTT
TAAACCTCTTGGACACTATCATTTCAGAAAACATCTAAAACCACTATTCATACTCTTTGCCATGACATGTGCCTCAACTA
TTTATACCAATCTGGATACAGTCATGCTTGGATTTATGGCTACAAATACCGATGTTGGCTATTATAATGCCGCAGTAAAG
ATAAAAGCCTTACTTGTAGCTGTTGTTACATCCCTAGGAACAGTATTGCTTCCAAGAGCTTCATATTACATTGAGAATGG
TTTTACATCCGAATTCAAAAAAATAAGCTCTAAAGCACTTAACTTCATATTTATTATTTCATTGCCAATGATAGTATACT
TTATTGTATTTGCAAAAGAATGTATTTTTTTCCTTTCAGGTACCAAATTCGTACCATCAACCATTCCAATGCAGATAATA
ATGCCTACTTTACTCTTTATAGGACTCACCAACATTCTTGGAATACAGATTCTTATTCCATTAGGTAAGGAACGTTATGT
TCTTTATTCAACTATTGTCGGGGCATTGGTAGACCTGATATTAAACGCAATACTGATTCCTCAATTCCAGTCAGCCGGTG
CCGCCATTGGAACTCTGGTAGCTGAATTTGCAGTATTTATTGTTCAATTTATACTTATTGATAGTGATATAAGAGAAATG
TTCAAAACTTTTCAAGTATACAAAATAGTAATCGCTGTTATTGCCGGGCTTCTTTGTTCTTGCTGGGTAAAGAACACATC
ATTACATGTATTCTTTACCCTTGCAATATCAGCTATTATATTCTTTCCAGTATATGGAATTGTTCTTTTATTATTAAAAG
AACAATTTATAACAGAAGTAAAAAATAATCTTTTATCCAAATTTAAAACTCATTAA

Upstream 100 bases:

>100_bases
AGCAATATCCGCAAACCTGTGTTATTATATTATACAAATCTTAAGAATTATAAGGAGAATCAATTTGAAAAAACAGGCAT
CACTTAAACTTAATTTCTTA

Downstream 100 bases:

>100_bases
TTATCTTCAATTGTTCTTATATCAGATAGTGGTAGCAATACTATCGCATTGACAGCCACATTTATAAGATGTGGCTCTGT
CATACTATGAACGAGAACAA

Product: O-antigen transporter

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MNIILTLSSIIFPLITFPYVSRVLQPEAWGKVNLATSFITYFSYFAQLGLPTYGVKICAKYRDDRLKLSRTVQELSIICT
ISTIITYVILGITIITIPKIASEKPLYILVSSTLFFNLIGMEWLYKALEKYTYITIRSLIFKFIALIGMFLLVKSQSDYI
IYGGISVFAASASNICNFIEARKYITFKPLGHYHFRKHLKPLFILFAMTCASTIYTNLDTVMLGFMATNTDVGYYNAAVK
IKALLVAVVTSLGTVLLPRASYYIENGFTSEFKKISSKALNFIFIISLPMIVYFIVFAKECIFFLSGTKFVPSTIPMQII
MPTLLFIGLTNILGIQILIPLGKERYVLYSTIVGALVDLILNAILIPQFQSAGAAIGTLVAEFAVFIVQFILIDSDIREM
FKTFQVYKIVIAVIAGLLCSCWVKNTSLHVFFTLAISAIIFFPVYGIVLLLLKEQFITEVKNNLLSKFKTH

Sequences:

>Translated_471_residues
MNIILTLSSIIFPLITFPYVSRVLQPEAWGKVNLATSFITYFSYFAQLGLPTYGVKICAKYRDDRLKLSRTVQELSIICT
ISTIITYVILGITIITIPKIASEKPLYILVSSTLFFNLIGMEWLYKALEKYTYITIRSLIFKFIALIGMFLLVKSQSDYI
IYGGISVFAASASNICNFIEARKYITFKPLGHYHFRKHLKPLFILFAMTCASTIYTNLDTVMLGFMATNTDVGYYNAAVK
IKALLVAVVTSLGTVLLPRASYYIENGFTSEFKKISSKALNFIFIISLPMIVYFIVFAKECIFFLSGTKFVPSTIPMQII
MPTLLFIGLTNILGIQILIPLGKERYVLYSTIVGALVDLILNAILIPQFQSAGAAIGTLVAEFAVFIVQFILIDSDIREM
FKTFQVYKIVIAVIAGLLCSCWVKNTSLHVFFTLAISAIIFFPVYGIVLLLLKEQFITEVKNNLLSKFKTH
>Mature_471_residues
MNIILTLSSIIFPLITFPYVSRVLQPEAWGKVNLATSFITYFSYFAQLGLPTYGVKICAKYRDDRLKLSRTVQELSIICT
ISTIITYVILGITIITIPKIASEKPLYILVSSTLFFNLIGMEWLYKALEKYTYITIRSLIFKFIALIGMFLLVKSQSDYI
IYGGISVFAASASNICNFIEARKYITFKPLGHYHFRKHLKPLFILFAMTCASTIYTNLDTVMLGFMATNTDVGYYNAAVK
IKALLVAVVTSLGTVLLPRASYYIENGFTSEFKKISSKALNFIFIISLPMIVYFIVFAKECIFFLSGTKFVPSTIPMQII
MPTLLFIGLTNILGIQILIPLGKERYVLYSTIVGALVDLILNAILIPQFQSAGAAIGTLVAEFAVFIVQFILIDSDIREM
FKTFQVYKIVIAVIAGLLCSCWVKNTSLHVFFTLAISAIIFFPVYGIVLLLLKEQFITEVKNNLLSKFKTH

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=340, Percent_Identity=24.4117647058824, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 52993; Mature: 52993

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIILTLSSIIFPLITFPYVSRVLQPEAWGKVNLATSFITYFSYFAQLGLPTYGVKICAK
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YRDDRLKLSRTVQELSIICTISTIITYVILGITIITIPKIASEKPLYILVSSTLFFNLIG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHH
MEWLYKALEKYTYITIRSLIFKFIALIGMFLLVKSQSDYIIYGGISVFAASASNICNFIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
ARKYITFKPLGHYHFRKHLKPLFILFAMTCASTIYTNLDTVMLGFMATNTDVGYYNAAVK
HCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IKALLVAVVTSLGTVLLPRASYYIENGFTSEFKKISSKALNFIFIISLPMIVYFIVFAKE
HHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CIFFLSGTKFVPSTIPMQIIMPTLLFIGLTNILGIQILIPLGKERYVLYSTIVGALVDLI
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHH
LNAILIPQFQSAGAAIGTLVAEFAVFIVQFILIDSDIREMFKTFQVYKIVIAVIAGLLCS
HHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CWVKNTSLHVFFTLAISAIIFFPVYGIVLLLLKEQFITEVKNNLLSKFKTH
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIILTLSSIIFPLITFPYVSRVLQPEAWGKVNLATSFITYFSYFAQLGLPTYGVKICAK
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
YRDDRLKLSRTVQELSIICTISTIITYVILGITIITIPKIASEKPLYILVSSTLFFNLIG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHH
MEWLYKALEKYTYITIRSLIFKFIALIGMFLLVKSQSDYIIYGGISVFAASASNICNFIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
ARKYITFKPLGHYHFRKHLKPLFILFAMTCASTIYTNLDTVMLGFMATNTDVGYYNAAVK
HCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
IKALLVAVVTSLGTVLLPRASYYIENGFTSEFKKISSKALNFIFIISLPMIVYFIVFAKE
HHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CIFFLSGTKFVPSTIPMQIIMPTLLFIGLTNILGIQILIPLGKERYVLYSTIVGALVDLI
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHH
LNAILIPQFQSAGAAIGTLVAEFAVFIVQFILIDSDIREMFKTFQVYKIVIAVIAGLLCS
HHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CWVKNTSLHVFFTLAISAIIFFPVYGIVLLLLKEQFITEVKNNLLSKFKTH
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]