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The map label for this gene is rfbX [H]

Identifier: 238924970

GI number: 238924970

Start: 2498909

End: 2500342

Strand: Direct

Name: rfbX [H]

Synonym: EUBREC_2621

Alternate gene names: 238924970

Gene position: 2498909-2500342 (Clockwise)

Preceding gene: 238924956

Following gene: 238924979

Centisome position: 72.44

GC content: 33.82

Gene sequence:

>1434_bases
ATGAAAAAGAAAATAAAAAAAACAAAAAGCATAAAATTAAACTACATATACAATGTAAGCTACCAAATACTTACACTAAT
CACGCCTCTGATTACAGCCCCGTATCTTTCGCGAGTGCTTAAGGTCGAAGGCATAGGTATATACAGCTATACCGCATCTA
TCGTTACATATTTTGTCATGTTTGCAGCCCTTGGCACCATCAATTATGGCAACAGGGAAATATCCTACCTACAAAATGAT
CGTCAAAATCGCTCAATCGTTTTCTGGGAAATAGAGTTTTTAAGTATCGGAAGTGTATTATGCTGTCTGGCAGCATACTC
ATTTTTTATACTATTTATAAACCATAAATATACAAGCTTATTTCTTGTACAGGCTTTTACTATCATTACTGTTGCAACAG
ACATATCTTGGTTGCTTCAGGGAATGGAAGAATTCGGTAAAATAGTTAGCAGAAATGTCGTATTTAAGATACTGAATATA
CTGTTTATTTTTATTTTCGTTAAGAGTCCAAATGACCTTTTAATTTACGTAGGTGGCTTATGTTTGCTCGAACTAGCGTC
CAATCTCTCAATATGGTTTTACGTTCCGCAATATATAAATCGACCAAATTTTAAACAATTACATCCTATAAGGCACCTTA
AACCAACAATAGCACTATTTATACCAACAATAGCATCTACCATCTATATCAATCTTGATAAAACAATGCTCAATGAAATT
ACCGGCTCACCTATCGAAAATGGCTACTACGAACAAGCATTCAAAATATATAAGGTTGCCTTATCGGTTGTAACCGCCCT
CGGTACTGTCATGCTGCCACGTATAGGAAAATGCTTCAGTGAAGGTAAAACCAACGAAGTAAAAAAACTGCTTTATAAAA
GCTATCGTTTTATATGGCTTATAGGATGTCCTATGTGCTGTGGATTAATTGGTGTTTCAAGAAACTTCTGTCCATGGTTC
TTCGGTCATGGGTACGATAAAGTCCCTTATATACTTATGATACAATCCGTTCTGCTTATCATAGTAGGATTAAGTAATGT
AACCGGTGTTCAATATCTTATCACAACAAAACGTGAATATCTCCTCACGCGATCTGTTTGTATAGGCGCTATAGCAAATT
TCATTATGAATTTAATTCTAATACCTAAATTTTATTCATATGGTGCTGCAGTGGCTTCAGTGCTATCAGAATTACTTATA
ACAATAATGCAATTTTATTTTGTTCGTAATGAAATAGACCTCAAATCTGTATTAAAATCATCACATAAATATATTATTGC
CTCTTTAACTATGCTAATATTTCTTTTGCTGGAGGATAATTATCTGCCAATAAACTTTGTAGGCACGGTCATCCTGGTTA
TTTCCGGATGTGCTGTTTATTTACTTATGCTCATTGTATTAAAAGATGAAATGTTAAAAGAACTTATTCCTTAA

Upstream 100 bases:

>100_bases
TGTCGGATTATATACGTGTAAGTTGATAGTAATGTTAATTTGCGATTTTTATATAATAACTGCATAGCCAAAATAAGTCA
ATATGTGATATGATATCTAT

Downstream 100 bases:

>100_bases
TACATTGAACAATATCTTTTACTGAGTCCAAATACTCTTCTTCATTATAAAAATAATTTGTCATGCATTCTCCCGAATCA
TGTAATCTGATAAAATTGGA

Product: polysaccharide biosynthesis protein

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKKKIKKTKSIKLNYIYNVSYQILTLITPLITAPYLSRVLKVEGIGIYSYTASIVTYFVMFAALGTINYGNREISYLQND
RQNRSIVFWEIEFLSIGSVLCCLAAYSFFILFINHKYTSLFLVQAFTIITVATDISWLLQGMEEFGKIVSRNVVFKILNI
LFIFIFVKSPNDLLIYVGGLCLLELASNLSIWFYVPQYINRPNFKQLHPIRHLKPTIALFIPTIASTIYINLDKTMLNEI
TGSPIENGYYEQAFKIYKVALSVVTALGTVMLPRIGKCFSEGKTNEVKKLLYKSYRFIWLIGCPMCCGLIGVSRNFCPWF
FGHGYDKVPYILMIQSVLLIIVGLSNVTGVQYLITTKREYLLTRSVCIGAIANFIMNLILIPKFYSYGAAVASVLSELLI
TIMQFYFVRNEIDLKSVLKSSHKYIIASLTMLIFLLLEDNYLPINFVGTVILVISGCAVYLLMLIVLKDEMLKELIP

Sequences:

>Translated_477_residues
MKKKIKKTKSIKLNYIYNVSYQILTLITPLITAPYLSRVLKVEGIGIYSYTASIVTYFVMFAALGTINYGNREISYLQND
RQNRSIVFWEIEFLSIGSVLCCLAAYSFFILFINHKYTSLFLVQAFTIITVATDISWLLQGMEEFGKIVSRNVVFKILNI
LFIFIFVKSPNDLLIYVGGLCLLELASNLSIWFYVPQYINRPNFKQLHPIRHLKPTIALFIPTIASTIYINLDKTMLNEI
TGSPIENGYYEQAFKIYKVALSVVTALGTVMLPRIGKCFSEGKTNEVKKLLYKSYRFIWLIGCPMCCGLIGVSRNFCPWF
FGHGYDKVPYILMIQSVLLIIVGLSNVTGVQYLITTKREYLLTRSVCIGAIANFIMNLILIPKFYSYGAAVASVLSELLI
TIMQFYFVRNEIDLKSVLKSSHKYIIASLTMLIFLLLEDNYLPINFVGTVILVISGCAVYLLMLIVLKDEMLKELIP
>Mature_477_residues
MKKKIKKTKSIKLNYIYNVSYQILTLITPLITAPYLSRVLKVEGIGIYSYTASIVTYFVMFAALGTINYGNREISYLQND
RQNRSIVFWEIEFLSIGSVLCCLAAYSFFILFINHKYTSLFLVQAFTIITVATDISWLLQGMEEFGKIVSRNVVFKILNI
LFIFIFVKSPNDLLIYVGGLCLLELASNLSIWFYVPQYINRPNFKQLHPIRHLKPTIALFIPTIASTIYINLDKTMLNEI
TGSPIENGYYEQAFKIYKVALSVVTALGTVMLPRIGKCFSEGKTNEVKKLLYKSYRFIWLIGCPMCCGLIGVSRNFCPWF
FGHGYDKVPYILMIQSVLLIIVGLSNVTGVQYLITTKREYLLTRSVCIGAIANFIMNLILIPKFYSYGAAVASVLSELLI
TIMQFYFVRNEIDLKSVLKSSHKYIIASLTMLIFLLLEDNYLPINFVGTVILVISGCAVYLLMLIVLKDEMLKELIP

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=414, Percent_Identity=24.3961352657005, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 54362; Mature: 54362

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIKKTKSIKLNYIYNVSYQILTLITPLITAPYLSRVLKVEGIGIYSYTASIVTYFVM
CCCCHHHHHCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FAALGTINYGNREISYLQNDRQNRSIVFWEIEFLSIGSVLCCLAAYSFFILFINHKYTSL
HHHHHCCCCCCCCHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
FLVQAFTIITVATDISWLLQGMEEFGKIVSRNVVFKILNILFIFIFVKSPNDLLIYVGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEHHHH
CLLELASNLSIWFYVPQYINRPNFKQLHPIRHLKPTIALFIPTIASTIYINLDKTMLNEI
HHHHHHCCCEEEEEEHHHCCCCCCHHCCCHHHCCHHHHHHHHHHHHEEEEEECHHHHHHH
TGSPIENGYYEQAFKIYKVALSVVTALGTVMLPRIGKCFSEGKTNEVKKLLYKSYRFIWL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEE
IGCPMCCGLIGVSRNFCPWFFGHGYDKVPYILMIQSVLLIIVGLSNVTGVQYLITTKREY
ECCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHEEEECHHHH
LLTRSVCIGAIANFIMNLILIPKFYSYGAAVASVLSELLITIMQFYFVRNEIDLKSVLKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SHKYIIASLTMLIFLLLEDNYLPINFVGTVILVISGCAVYLLMLIVLKDEMLKELIP
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKIKKTKSIKLNYIYNVSYQILTLITPLITAPYLSRVLKVEGIGIYSYTASIVTYFVM
CCCCHHHHHCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FAALGTINYGNREISYLQNDRQNRSIVFWEIEFLSIGSVLCCLAAYSFFILFINHKYTSL
HHHHHCCCCCCCCHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
FLVQAFTIITVATDISWLLQGMEEFGKIVSRNVVFKILNILFIFIFVKSPNDLLIYVGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEHHHH
CLLELASNLSIWFYVPQYINRPNFKQLHPIRHLKPTIALFIPTIASTIYINLDKTMLNEI
HHHHHHCCCEEEEEEHHHCCCCCCHHCCCHHHCCHHHHHHHHHHHHEEEEEECHHHHHHH
TGSPIENGYYEQAFKIYKVALSVVTALGTVMLPRIGKCFSEGKTNEVKKLLYKSYRFIWL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEE
IGCPMCCGLIGVSRNFCPWFFGHGYDKVPYILMIQSVLLIIVGLSNVTGVQYLITTKREY
ECCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHEEEECHHHH
LLTRSVCIGAIANFIMNLILIPKFYSYGAAVASVLSELLITIMQFYFVRNEIDLKSVLKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SHKYIIASLTMLIFLLLEDNYLPINFVGTVILVISGCAVYLLMLIVLKDEMLKELIP
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]