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The map label for this gene is purR [H]

Identifier: 238924910

GI number: 238924910

Start: 2434836

End: 2435894

Strand: Direct

Name: purR [H]

Synonym: EUBREC_2561

Alternate gene names: 238924910

Gene position: 2434836-2435894 (Clockwise)

Preceding gene: 238924896

Following gene: 238924920

Centisome position: 70.58

GC content: 44.1

Gene sequence:

>1059_bases
ATGTCTATCACAGCAAAAGAACTTGCCAAACAACTTAATCTTTCTGAAGCAGCTATATCGATGGCACTTAATAATAAGCC
CGGTGTCAGCACACTCACCAGAAAACGTGTGCTCGAAGCTGCGGCCTCTGCCGGATATGATTTTTCAAGAATATCAAATA
CCAATGAATCTCCGGCCTCCAATGGCACATTATATTTTGTAATATACAGAAAGAATGGCGCTGTAGTTCCTAATTCTCCT
GTTTACACACATACAGAGCACGGCGTTTTGGTACAGGATGTTCCATTCTTTTCACAGCTGTCAGAGGGTATTGATTTGGG
CTGCAGGCACTGCCACTACTACCTGAATATCAGCTACATATACGAAAATGACGACATTGAGGCTTTGCTATCTGAGTGGA
AAAGGCTTGGAGCAAAGGGTATCCTGCTGCTTGGAACCGAGATGGAAGAGCATGACATAAAGCCCTTTACAAGGTGCGGT
CTGCCTGTTGTGCTAATAGACAACTATTTCGAAGCCTTAAACCTCGATTGCGTGACTATAAATAACCTTCAGGGTGCCTA
TCTTGCCACAGACTACCTGATCAAGCAAACACATGCACAACCCGGATATCTGCATTCCGCATACAGTATAACCGGCTTTG
AGGAAAGAGCCGACGGCTTCTATAAAGCAATACGCAAAAACGGTATGTCAACCTCGCGCTCAGTCGTACATCACCTCTCC
CCTTCAGTTGACGGTGCTTACTGTGATATGAAGGCAGTTATAAAAAGCGGTGATGAGCTTGTCCGATGCTACTTTGCTGA
CAATGACCTGATTGCTGCCGGAGCTATGCGTGCACTTTCAGAGGCAGGCTACCGTATTCCTGAAGACATATCAGTAATAG
GCTTTGATGATATGCCAATGTGTACCTACATTACACCGCCTCTTTCGACTGTGCATGTGCCGAAGCAGTACATGGGTGAA
ATTGCAGTAAAAAGGCTTGCAGAGATAATAAATTCTACCTCTGCAAGCCATGTAAAAATTGAAATAAGTACAGAAATTGT
AAAGAGAAAGAGCTGTTAA

Upstream 100 bases:

>100_bases
AGTAAATTTAGTGTATTTTTCAACTTAATTTACTAAATTATAATGTTAATACTATATTGTCATGTTATAATGAGACAAAT
AAAACATAAGGAGCCATGCC

Downstream 100 bases:

>100_bases
TTTCTATACATTTGCTTATGCATTTTTCTGAACTGCCGATGCACTAAGCTTGCCATCCTTCACATCTATAAGAATGGTAT
CTCCTGCTCTCACACCATCT

Product: transcriptional regulator, LacI family

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSITAKELAKQLNLSEAAISMALNNKPGVSTLTRKRVLEAAASAGYDFSRISNTNESPASNGTLYFVIYRKNGAVVPNSP
VYTHTEHGVLVQDVPFFSQLSEGIDLGCRHCHYYLNISYIYENDDIEALLSEWKRLGAKGILLLGTEMEEHDIKPFTRCG
LPVVLIDNYFEALNLDCVTINNLQGAYLATDYLIKQTHAQPGYLHSAYSITGFEERADGFYKAIRKNGMSTSRSVVHHLS
PSVDGAYCDMKAVIKSGDELVRCYFADNDLIAAGAMRALSEAGYRIPEDISVIGFDDMPMCTYITPPLSTVHVPKQYMGE
IAVKRLAEIINSTSASHVKIEISTEIVKRKSC

Sequences:

>Translated_352_residues
MSITAKELAKQLNLSEAAISMALNNKPGVSTLTRKRVLEAAASAGYDFSRISNTNESPASNGTLYFVIYRKNGAVVPNSP
VYTHTEHGVLVQDVPFFSQLSEGIDLGCRHCHYYLNISYIYENDDIEALLSEWKRLGAKGILLLGTEMEEHDIKPFTRCG
LPVVLIDNYFEALNLDCVTINNLQGAYLATDYLIKQTHAQPGYLHSAYSITGFEERADGFYKAIRKNGMSTSRSVVHHLS
PSVDGAYCDMKAVIKSGDELVRCYFADNDLIAAGAMRALSEAGYRIPEDISVIGFDDMPMCTYITPPLSTVHVPKQYMGE
IAVKRLAEIINSTSASHVKIEISTEIVKRKSC
>Mature_351_residues
SITAKELAKQLNLSEAAISMALNNKPGVSTLTRKRVLEAAASAGYDFSRISNTNESPASNGTLYFVIYRKNGAVVPNSPV
YTHTEHGVLVQDVPFFSQLSEGIDLGCRHCHYYLNISYIYENDDIEALLSEWKRLGAKGILLLGTEMEEHDIKPFTRCGL
PVVLIDNYFEALNLDCVTINNLQGAYLATDYLIKQTHAQPGYLHSAYSITGFEERADGFYKAIRKNGMSTSRSVVHHLSP
SVDGAYCDMKAVIKSGDELVRCYFADNDLIAAGAMRALSEAGYRIPEDISVIGFDDMPMCTYITPPLSTVHVPKQYMGEI
AVKRLAEIINSTSASHVKIEISTEIVKRKSC

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=316, Percent_Identity=28.4810126582279, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788474, Length=329, Percent_Identity=27.9635258358663, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790194, Length=264, Percent_Identity=27.6515151515151, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787948, Length=352, Percent_Identity=25, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790369, Length=339, Percent_Identity=25.6637168141593, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789068, Length=379, Percent_Identity=24.0105540897098, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787580, Length=357, Percent_Identity=21.2885154061625, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI48994940, Length=352, Percent_Identity=24.1477272727273, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787906, Length=348, Percent_Identity=22.4137931034483, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38885; Mature: 38754

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITAKELAKQLNLSEAAISMALNNKPGVSTLTRKRVLEAAASAGYDFSRISNTNESPAS
CCCCHHHHHHHCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCC
NGTLYFVIYRKNGAVVPNSPVYTHTEHGVLVQDVPFFSQLSEGIDLGCRHCHYYLNISYI
CCEEEEEEECCCCCCCCCCCCEEECCCCEEEECCCHHHHHHHHHHHCCCEEEEEEEEEEE
YENDDIEALLSEWKRLGAKGILLLGTEMEEHDIKPFTRCGLPVVLIDNYFEALNLDCVTI
EECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCEEECCCHHHHHCCEEEEE
NNLQGAYLATDYLIKQTHAQPGYLHSAYSITGFEERADGFYKAIRKNGMSTSRSVVHHLS
CCCCCCHHHHHHHHHHCCCCCCCEECEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHCC
PSVDGAYCDMKAVIKSGDELVRCYFADNDLIAAGAMRALSEAGYRIPEDISVIGFDDMPM
CCCCCHHHHHHHHHHCCCEEEEEEECCCCEEHHHHHHHHHHCCCCCCCCCEEEECCCCCE
CTYITPPLSTVHVPKQYMGEIAVKRLAEIINSTSASHVKIEISTEIVKRKSC
EEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHCCC
>Mature Secondary Structure 
SITAKELAKQLNLSEAAISMALNNKPGVSTLTRKRVLEAAASAGYDFSRISNTNESPAS
CCCHHHHHHHCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCC
NGTLYFVIYRKNGAVVPNSPVYTHTEHGVLVQDVPFFSQLSEGIDLGCRHCHYYLNISYI
CCEEEEEEECCCCCCCCCCCCEEECCCCEEEECCCHHHHHHHHHHHCCCEEEEEEEEEEE
YENDDIEALLSEWKRLGAKGILLLGTEMEEHDIKPFTRCGLPVVLIDNYFEALNLDCVTI
EECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCEEECCCHHHHHCCEEEEE
NNLQGAYLATDYLIKQTHAQPGYLHSAYSITGFEERADGFYKAIRKNGMSTSRSVVHHLS
CCCCCCHHHHHHHHHHCCCCCCCEECEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHCC
PSVDGAYCDMKAVIKSGDELVRCYFADNDLIAAGAMRALSEAGYRIPEDISVIGFDDMPM
CCCCCHHHHHHHHHHCCCEEEEEEECCCCEEHHHHHHHHHHCCCCCCCCCEEEECCCCCE
CTYITPPLSTVHVPKQYMGEIAVKRLAEIINSTSASHVKIEISTEIVKRKSC
EEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]