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The map label for this gene is pfp [H]

Identifier: 238924666

GI number: 238924666

Start: 2177604

End: 2178842

Strand: Direct

Name: pfp [H]

Synonym: EUBREC_2314

Alternate gene names: 238924666

Gene position: 2177604-2178842 (Clockwise)

Preceding gene: 238924661

Following gene: 238924667

Centisome position: 63.12

GC content: 44.31

Gene sequence:

>1239_bases
ATGAATCAGTCAAACTGTATCGTCGCCCAGTCAGGCGGCCCAACTGTTGCAATCAACGCAAGCCTTGCAGGAGTCATCGC
AGGAGTAAAAAGCTCTGAGAAATACGATATCTGCTATGGCGCCGTAAACGGAATACTCGGCATATTAAATGAGAATTATC
TCAATCTGTCAGATATTTTTGCAGATGATGCTAATCTTGAAAAGCTTAAGACCACACCTGCCATGTATCTTGGCTCATGC
CGCCACAAACTCCCTGATTACAAGGATGATGATTCACCATATGTATACATCTTCCACCAGTTCGATAAGCTTTCCATAAA
AGCTTTCTTTTACATCGGTGGAAATGATTCGATGGATACCGTATTAAAGTTATCCGATTATGCAAAAAAAATCAACAGTG
ATGTGAAAATTATAGGGATTCCAAAAACCATCGACAATGATCTGTGCATGACAGACCACACTCCTGGCTTTGGCTCTGCC
GCCAAATATATCGCATCAAGCATGCTTGAGATAGCCCATGATACCTTTATCTATGCTGTTAAGAGTGTGACAATCGTTGA
AATCATGGGACGTGACGCAGGATGGCTCACTGCCGCTTCCGCTCTCGCACGTAATGAGTACAATACAGCGCCTCAGTTCA
TCTATCTGCCTGAGGTGAATTTCGACAAGGACAAATTTGTTGCCGATATAAAAGAAGCCCTTGCGCACACTAACAATGTA
ATAGTCGCCGTATCCGAAGGAATACATGACGCAGACGGCTCATACATTACATCATCAAAGGCCGCATGTGACCAGTTCGG
ACACCAGCAGCTAAGCGGTGCCGGAAAAGCTCTTGAGTTCATCGTAAAGGAAAACATCGGTGTCAAGGTGCGCTCTGTAG
AGGTAAATGTGCTCCAGCGCTGTGCCTCACATCTTGCATCATTAACCGATATCGAGGAATCTTTTGCTCAGGGTAAAAAG
GGTGTCACTATTGCAGCAGAGGGTGTGACCGCATCAATGGTATGCTTAAAGCGCATCTCAAATAAGCCTTATCAGGTGGA
ATACACATATTCTGCTATCAAGGATATCGCAAACGAGGCCAAGTCTGTGCCAAGAGAGTGGATCAATGATGCTGGCAATG
ATGTGAAACCTGAACTTATCGAATACTTAAAGCCACTTATTCAGGGAGAAACCGCTGTAGCCTACAAGAATGGTCTGCCT
GACTATATGGATGTGTCACATCTTCACAAATGTAACTGA

Upstream 100 bases:

>100_bases
AATGCGTAGCATGTAGTAATCTGATTAATCATCATTTTAAATTATATCATAACTATTCTTACAGAAGAGAATAGTTAAAA
TACACAATAAGGAGATTACA

Downstream 100 bases:

>100_bases
TTTTAGCTGCTGTCTACACCCCGGTGTGGGCAGCAACAGCATATCTCCAAGGAGGTCGATTTACATGAAGAAAAAAGTGC
TTATAACCTGCGCCGTTGTG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MNQSNCIVAQSGGPTVAINASLAGVIAGVKSSEKYDICYGAVNGILGILNENYLNLSDIFADDANLEKLKTTPAMYLGSC
RHKLPDYKDDDSPYVYIFHQFDKLSIKAFFYIGGNDSMDTVLKLSDYAKKINSDVKIIGIPKTIDNDLCMTDHTPGFGSA
AKYIASSMLEIAHDTFIYAVKSVTIVEIMGRDAGWLTAASALARNEYNTAPQFIYLPEVNFDKDKFVADIKEALAHTNNV
IVAVSEGIHDADGSYITSSKAACDQFGHQQLSGAGKALEFIVKENIGVKVRSVEVNVLQRCASHLASLTDIEESFAQGKK
GVTIAAEGVTASMVCLKRISNKPYQVEYTYSAIKDIANEAKSVPREWINDAGNDVKPELIEYLKPLIQGETAVAYKNGLP
DYMDVSHLHKCN

Sequences:

>Translated_412_residues
MNQSNCIVAQSGGPTVAINASLAGVIAGVKSSEKYDICYGAVNGILGILNENYLNLSDIFADDANLEKLKTTPAMYLGSC
RHKLPDYKDDDSPYVYIFHQFDKLSIKAFFYIGGNDSMDTVLKLSDYAKKINSDVKIIGIPKTIDNDLCMTDHTPGFGSA
AKYIASSMLEIAHDTFIYAVKSVTIVEIMGRDAGWLTAASALARNEYNTAPQFIYLPEVNFDKDKFVADIKEALAHTNNV
IVAVSEGIHDADGSYITSSKAACDQFGHQQLSGAGKALEFIVKENIGVKVRSVEVNVLQRCASHLASLTDIEESFAQGKK
GVTIAAEGVTASMVCLKRISNKPYQVEYTYSAIKDIANEAKSVPREWINDAGNDVKPELIEYLKPLIQGETAVAYKNGLP
DYMDVSHLHKCN
>Mature_412_residues
MNQSNCIVAQSGGPTVAINASLAGVIAGVKSSEKYDICYGAVNGILGILNENYLNLSDIFADDANLEKLKTTPAMYLGSC
RHKLPDYKDDDSPYVYIFHQFDKLSIKAFFYIGGNDSMDTVLKLSDYAKKINSDVKIIGIPKTIDNDLCMTDHTPGFGSA
AKYIASSMLEIAHDTFIYAVKSVTIVEIMGRDAGWLTAASALARNEYNTAPQFIYLPEVNFDKDKFVADIKEALAHTNNV
IVAVSEGIHDADGSYITSSKAACDQFGHQQLSGAGKALEFIVKENIGVKVRSVEVNVLQRCASHLASLTDIEESFAQGKK
GVTIAAEGVTASMVCLKRISNKPYQVEYTYSAIKDIANEAKSVPREWINDAGNDVKPELIEYLKPLIQGETAVAYKNGLP
DYMDVSHLHKCN

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=286, Percent_Identity=27.6223776223776, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 44915; Mature: 44915

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSNCIVAQSGGPTVAINASLAGVIAGVKSSEKYDICYGAVNGILGILNENYLNLSDIF
CCCCCEEEEECCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
ADDANLEKLKTTPAMYLGSCRHKLPDYKDDDSPYVYIFHQFDKLSIKAFFYIGGNDSMDT
CCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEEEECCCCCHHH
VLKLSDYAKKINSDVKIIGIPKTIDNDLCMTDHTPGFGSAAKYIASSMLEIAHDTFIYAV
HHHHHHHHHHHCCCEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KSVTIVEIMGRDAGWLTAASALARNEYNTAPQFIYLPEVNFDKDKFVADIKEALAHTNNV
HHEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCE
IVAVSEGIHDADGSYITSSKAACDQFGHQQLSGAGKALEFIVKENIGVKVRSVEVNVLQR
EEEEECCCCCCCCCEEECCHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEEEHHHHHHH
CASHLASLTDIEESFAQGKKGVTIAAEGVTASMVCLKRISNKPYQVEYTYSAIKDIANEA
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
KSVPREWINDAGNDVKPELIEYLKPLIQGETAVAYKNGLPDYMDVSHLHKCN
HHCCHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHCHHHHHCCC
>Mature Secondary Structure
MNQSNCIVAQSGGPTVAINASLAGVIAGVKSSEKYDICYGAVNGILGILNENYLNLSDIF
CCCCCEEEEECCCCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
ADDANLEKLKTTPAMYLGSCRHKLPDYKDDDSPYVYIFHQFDKLSIKAFFYIGGNDSMDT
CCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEEEECCCCCHHH
VLKLSDYAKKINSDVKIIGIPKTIDNDLCMTDHTPGFGSAAKYIASSMLEIAHDTFIYAV
HHHHHHHHHHHCCCEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KSVTIVEIMGRDAGWLTAASALARNEYNTAPQFIYLPEVNFDKDKFVADIKEALAHTNNV
HHEEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCE
IVAVSEGIHDADGSYITSSKAACDQFGHQQLSGAGKALEFIVKENIGVKVRSVEVNVLQR
EEEEECCCCCCCCCEEECCHHHHHHHHHHHHHCHHHHHHHHHHCCCCEEEEEEHHHHHHH
CASHLASLTDIEESFAQGKKGVTIAAEGVTASMVCLKRISNKPYQVEYTYSAIKDIANEA
HHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
KSVPREWINDAGNDVKPELIEYLKPLIQGETAVAYKNGLPDYMDVSHLHKCN
HHCCHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]