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The map label for this gene is potB [H]

Identifier: 238922326

GI number: 238922326

Start: 531130

End: 531915

Strand: Reverse

Name: potB [H]

Synonym: EUBELI_20562

Alternate gene names: 238922326

Gene position: 531915-531130 (Counterclockwise)

Preceding gene: 238922327

Following gene: 238922325

Centisome position: 84.87

GC content: 36.64

Gene sequence:

>786_bases
ATGTCTTTTTCACGCAAATATCTTGGAATCCCTTATGGGCTGTTTCTTATATTATTTGTTGCAGCACCACTGCTTGTCCT
TGTATATTATGCATTTACTAACGGGCAGGGACAATTCAGCATGGTTAATCTGCAGAATTTTTTTACAGACCCTAATACAC
TTGGAACTCTGTGTTACAGTCTTGTACTTGCATTTGTTGCAACTGCAGTCTGTCTTATAATTGCTTATCCTGTAGCGTAT
ATACTTTCACAGAGTAAGTTAAAGAGAAAAGCGGTAATACTTCTGCTTTTTGTTATGCCTATGTGGATTAATTTCACATT
AAGAATTACCGCACTTAAAGAGATATTATCAATGATTGAGGGAAATCTTGCGTATTATCCGTTTATCAATACGATAATAG
GAATGACATACGATTTTCTTCCATTCATGATACTTCCGTTATATACAACGCTTTCAAAGCTTGATAAGAGTGTTATTGAA
GCAGCATCGGATCTTGGTGCGGATAATTTCCAGACATTTATCAAGGTTATACTGCCATTGTCTGTTCCGGGAATTGTCAG
TGGTGTGTCAATGGTATTCCTTCCGGCAATGACTAACTATGTAGTTCTTGATATGCTTTATAACAGCACATATATTATGG
GTAGTCTGATTGGAAGTTATTTCAGTGCATATGACTGGCACAATGGCTCAATGATAGCACTGATACTTCTTGTTATTATA
TGTATTGTCACTCTGGTTTCGGGTGATAAAGAGTCAGAAAATGCTGGAAGAGGAGGACTTGTATAA

Upstream 100 bases:

>100_bases
TGTAGATGATGAATATCTCTGGAATATGGATGATCAGGTTGGTCTTATCATGCCTGAAGAGAAGATGAAATTCCAGTTGA
AGAAGTAGGGGGATAGAAAT

Downstream 100 bases:

>100_bases
TGAAGAAATTTCTGAAATATATGATTATATGCATTGTCCTTTTGTTCATGTATATTCCAATTCTTGTGCTTGCAGTATAC
AGTTTTACGGATGCCGCTAA

Product: spermidine/putrescine transport system permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSFSRKYLGIPYGLFLILFVAAPLLVLVYYAFTNGQGQFSMVNLQNFFTDPNTLGTLCYSLVLAFVATAVCLIIAYPVAY
ILSQSKLKRKAVILLLFVMPMWINFTLRITALKEILSMIEGNLAYYPFINTIIGMTYDFLPFMILPLYTTLSKLDKSVIE
AASDLGADNFQTFIKVILPLSVPGIVSGVSMVFLPAMTNYVVLDMLYNSTYIMGSLIGSYFSAYDWHNGSMIALILLVII
CIVTLVSGDKESENAGRGGLV

Sequences:

>Translated_261_residues
MSFSRKYLGIPYGLFLILFVAAPLLVLVYYAFTNGQGQFSMVNLQNFFTDPNTLGTLCYSLVLAFVATAVCLIIAYPVAY
ILSQSKLKRKAVILLLFVMPMWINFTLRITALKEILSMIEGNLAYYPFINTIIGMTYDFLPFMILPLYTTLSKLDKSVIE
AASDLGADNFQTFIKVILPLSVPGIVSGVSMVFLPAMTNYVVLDMLYNSTYIMGSLIGSYFSAYDWHNGSMIALILLVII
CIVTLVSGDKESENAGRGGLV
>Mature_260_residues
SFSRKYLGIPYGLFLILFVAAPLLVLVYYAFTNGQGQFSMVNLQNFFTDPNTLGTLCYSLVLAFVATAVCLIIAYPVAYI
LSQSKLKRKAVILLLFVMPMWINFTLRITALKEILSMIEGNLAYYPFINTIIGMTYDFLPFMILPLYTTLSKLDKSVIEA
ASDLGADNFQTFIKVILPLSVPGIVSGVSMVFLPAMTNYVVLDMLYNSTYIMGSLIGSYFSAYDWHNGSMIALILLVIIC
IVTLVSGDKESENAGRGGLV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=253, Percent_Identity=35.5731225296443, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787080, Length=251, Percent_Identity=32.2709163346614, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787713, Length=234, Percent_Identity=25.6410256410256, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28878; Mature: 28747

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFSRKYLGIPYGLFLILFVAAPLLVLVYYAFTNGQGQFSMVNLQNFFTDPNTLGTLCYS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCHHHHHHHHH
LVLAFVATAVCLIIAYPVAYILSQSKLKRKAVILLLFVMPMWINFTLRITALKEILSMIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GNLAYYPFINTIIGMTYDFLPFMILPLYTTLSKLDKSVIEAASDLGADNFQTFIKVILPL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
SVPGIVSGVSMVFLPAMTNYVVLDMLYNSTYIMGSLIGSYFSAYDWHNGSMIALILLVII
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
CIVTLVSGDKESENAGRGGLV
HHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]