Click here to switch to the map view.

The map label for this gene is potD-A [H]

Identifier: 238922324

GI number: 238922324

Start: 528808

End: 530328

Strand: Reverse

Name: potD-A [H]

Synonym: EUBELI_20560

Alternate gene names: 238922324

Gene position: 530328-528808 (Counterclockwise)

Preceding gene: 238922325

Following gene: 238922323

Centisome position: 84.62

GC content: 37.54

Gene sequence:

>1521_bases
ATGAATAAGATTATTGGAAAGATAAATGCGTTAATACTGTCAGCTTTAATGGCAGTAAGCGGCGCAGCAGCGGTAACAGG
AGTGTTTAATACAAACAGTCTTACAGCGGTGGCAGCAGACGATACAATCATACTCCGTGTGTGCAACTGGGAAGAATATA
TTGATACAGGAGACTGGAGTGATGATGATATAATTGACCTTGAGAGCGGAAGCATAAAGGGTGAGAATTCGATAATTACT
GATTTCGAGAACTGGTATTATGAGAATTATGGCAAGAAAGTTAAGGTACAGTACAGCTGTCTTGGAACTAACGAGGAGTT
GTACAACATGCTTACTCTGGGTGATGATTACGATGTAATCTGTCCTTCAGAATATATGATAATGAAGCTTATGGCAGAGG
ACTGGCTTGAGCCATTTTCAGATGATTTCTATGATACATCGATTGCTAATAATTATTATGCTAAGGGTGTATCTCCATTT
ATCAAGAAAACATTTGACGAGAATAAGATTAATGGTGAGTCATGGAGCCGCTATGCTGCAGGATATATGTGGGGTATAAC
AGGAATCGTGTACAATCCTGATAAGGTGACAGAAAAAGAAGCATCTACATGGACGATAATTGATAACAGCAAATTCAGAC
GTCAGATAACAATTAAGGATAATGTAAGAGATTCCATGTTTGCAGCTGTTGGTGCAATTAAGTCAGACAAGTTAAAGTCA
GCGTCATTCATTAACAGTCCTGATTATCATGAAAGACTTGCCGAGGAGATGAATGATACTTCTGAGGCAAATGTGGACAG
GATTCAGGAGTATCTTCAGTCAGTTAAGAATAATGCCTATTCATTCGAGACTGACAGTGCCAAGACTGATATGATAACAG
GAAAGGTAGTCGCAGGTTATCAGTGGTCTGGAGATGCTGTATATACTATGGACCAGGCTGACGAGGATGATTTCCAGCTT
AACTTTGCAGTACCTGAAGAATGTACTAACCTTTATTTTGACGGCTGGGTAATGTTAAAGTCTGGAATTAATGGCAATTC
AGAGAAAAAGCAGGCAGCACAGAGCTTTATTAACTTTCTGTCTAAACCTGAAAATGCTGTAAGAAACATGAGCTATATTG
GATATACATCAGTTATATCAGGTGGTGACAGTGATGTTGTATTCGATTATGTAAAGTGGAATTATGGAGCAGATGAAAGT
GATACAGATGTTGTAGATTATCCTCTGGGATATTTCTTTTCAGGTGATTCTGATGATGAAAGATATATACTTAAAGTTCC
AGAAGAACAGACATACAGACAGTTATCAGCACAGTATCCTACGCAGGAGGTTATGGATAGAAGTGCCATTATGCAGTATT
TTGATGCTGAAGAGACAACAAGAATTAATCAGATGTGGATTAATGTAAGATGTTATAACATTAAGAATGTTCCTGTGTGG
ATATGGGTTCTTGCAGGTATTATTGTTGTGACTTTAATAGCATTATCTGTAAAACTTAAGATAAATAAGAAATATGATTA
A

Upstream 100 bases:

>100_bases
TGGGCTTTATCTACAATAATATTCCTTATTGTTATTGTAGTTGAGATAGCAATTAATCTTGCACCAGCAAGAAAAGCTGT
ACGAGAGGAGGCCGGAGCTG

Downstream 100 bases:

>100_bases
TAAGCATTTGATTATAAATTGACAAATAACCAAATGTTGCTATTATATATGTCAGACAGAATGAATCTGTATGACAGTGC
AAAGGAGCACAGAATTTCTG

Product: spermidine/putrescine transport system substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MNKIIGKINALILSALMAVSGAAAVTGVFNTNSLTAVAADDTIILRVCNWEEYIDTGDWSDDDIIDLESGSIKGENSIIT
DFENWYYENYGKKVKVQYSCLGTNEELYNMLTLGDDYDVICPSEYMIMKLMAEDWLEPFSDDFYDTSIANNYYAKGVSPF
IKKTFDENKINGESWSRYAAGYMWGITGIVYNPDKVTEKEASTWTIIDNSKFRRQITIKDNVRDSMFAAVGAIKSDKLKS
ASFINSPDYHERLAEEMNDTSEANVDRIQEYLQSVKNNAYSFETDSAKTDMITGKVVAGYQWSGDAVYTMDQADEDDFQL
NFAVPEECTNLYFDGWVMLKSGINGNSEKKQAAQSFINFLSKPENAVRNMSYIGYTSVISGGDSDVVFDYVKWNYGADES
DTDVVDYPLGYFFSGDSDDERYILKVPEEQTYRQLSAQYPTQEVMDRSAIMQYFDAEETTRINQMWINVRCYNIKNVPVW
IWVLAGIIVVTLIALSVKLKINKKYD

Sequences:

>Translated_506_residues
MNKIIGKINALILSALMAVSGAAAVTGVFNTNSLTAVAADDTIILRVCNWEEYIDTGDWSDDDIIDLESGSIKGENSIIT
DFENWYYENYGKKVKVQYSCLGTNEELYNMLTLGDDYDVICPSEYMIMKLMAEDWLEPFSDDFYDTSIANNYYAKGVSPF
IKKTFDENKINGESWSRYAAGYMWGITGIVYNPDKVTEKEASTWTIIDNSKFRRQITIKDNVRDSMFAAVGAIKSDKLKS
ASFINSPDYHERLAEEMNDTSEANVDRIQEYLQSVKNNAYSFETDSAKTDMITGKVVAGYQWSGDAVYTMDQADEDDFQL
NFAVPEECTNLYFDGWVMLKSGINGNSEKKQAAQSFINFLSKPENAVRNMSYIGYTSVISGGDSDVVFDYVKWNYGADES
DTDVVDYPLGYFFSGDSDDERYILKVPEEQTYRQLSAQYPTQEVMDRSAIMQYFDAEETTRINQMWINVRCYNIKNVPVW
IWVLAGIIVVTLIALSVKLKINKKYD
>Mature_506_residues
MNKIIGKINALILSALMAVSGAAAVTGVFNTNSLTAVAADDTIILRVCNWEEYIDTGDWSDDDIIDLESGSIKGENSIIT
DFENWYYENYGKKVKVQYSCLGTNEELYNMLTLGDDYDVICPSEYMIMKLMAEDWLEPFSDDFYDTSIANNYYAKGVSPF
IKKTFDENKINGESWSRYAAGYMWGITGIVYNPDKVTEKEASTWTIIDNSKFRRQITIKDNVRDSMFAAVGAIKSDKLKS
ASFINSPDYHERLAEEMNDTSEANVDRIQEYLQSVKNNAYSFETDSAKTDMITGKVVAGYQWSGDAVYTMDQADEDDFQL
NFAVPEECTNLYFDGWVMLKSGINGNSEKKQAAQSFINFLSKPENAVRNMSYIGYTSVISGGDSDVVFDYVKWNYGADES
DTDVVDYPLGYFFSGDSDDERYILKVPEEQTYRQLSAQYPTQEVMDRSAIMQYFDAEETTRINQMWINVRCYNIKNVPVW
IWVLAGIIVVTLIALSVKLKINKKYD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=349, Percent_Identity=22.9226361031519, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 57501; Mature: 57501

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIIGKINALILSALMAVSGAAAVTGVFNTNSLTAVAADDTIILRVCNWEEYIDTGDWS
CCCHHHHHHHHHHHHHHHHCCCHHEEEEECCCCEEEEECCCEEEEEEECHHHHHCCCCCC
DDDIIDLESGSIKGENSIITDFENWYYENYGKKVKVQYSCLGTNEELYNMLTLGDDYDVI
CCCEEEECCCCCCCCCCEEECHHHHHHHCCCCEEEEEEEEECCCHHHHHHEECCCCCCEE
CPSEYMIMKLMAEDWLEPFSDDFYDTSIANNYYAKGVSPFIKKTFDENKINGESWSRYAA
CCCHHHHHHHHHHHHHCHHCCCCCCHHHCCCHHHCCCCHHHHHHCCCCCCCCCHHHHHHH
GYMWGITGIVYNPDKVTEKEASTWTIIDNSKFRRQITIKDNVRDSMFAAVGAIKSDKLKS
HHHEEEEEEEECCCCCCCCCCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHCCCCCCC
ASFINSPDYHERLAEEMNDTSEANVDRIQEYLQSVKNNAYSFETDSAKTDMITGKVVAGY
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEECEEEEEE
QWSGDAVYTMDQADEDDFQLNFAVPEECTNLYFDGWVMLKSGINGNSEKKQAAQSFINFL
EECCCEEEEECCCCCCCEEEEEECCHHHHCEEECCEEEEECCCCCCHHHHHHHHHHHHHH
SKPENAVRNMSYIGYTSVISGGDSDVVFDYVKWNYGADESDTDVVDYPLGYFFSGDSDDE
CCCHHHHHCCHHCCHHHEECCCCCCEEEEEEEECCCCCCCCCCEEECCCCEEECCCCCCC
RYILKVPEEQTYRQLSAQYPTQEVMDRSAIMQYFDAEETTRINQMWINVRCYNIKNVPVW
EEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCEEEEEEEEEEEEEECCCCHH
IWVLAGIIVVTLIALSVKLKINKKYD
HHHHHHHHHHHHHHHHEEEEEECCCC
>Mature Secondary Structure
MNKIIGKINALILSALMAVSGAAAVTGVFNTNSLTAVAADDTIILRVCNWEEYIDTGDWS
CCCHHHHHHHHHHHHHHHHCCCHHEEEEECCCCEEEEECCCEEEEEEECHHHHHCCCCCC
DDDIIDLESGSIKGENSIITDFENWYYENYGKKVKVQYSCLGTNEELYNMLTLGDDYDVI
CCCEEEECCCCCCCCCCEEECHHHHHHHCCCCEEEEEEEEECCCHHHHHHEECCCCCCEE
CPSEYMIMKLMAEDWLEPFSDDFYDTSIANNYYAKGVSPFIKKTFDENKINGESWSRYAA
CCCHHHHHHHHHHHHHCHHCCCCCCHHHCCCHHHCCCCHHHHHHCCCCCCCCCHHHHHHH
GYMWGITGIVYNPDKVTEKEASTWTIIDNSKFRRQITIKDNVRDSMFAAVGAIKSDKLKS
HHHEEEEEEEECCCCCCCCCCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHCCCCCCC
ASFINSPDYHERLAEEMNDTSEANVDRIQEYLQSVKNNAYSFETDSAKTDMITGKVVAGY
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEECEEEEEE
QWSGDAVYTMDQADEDDFQLNFAVPEECTNLYFDGWVMLKSGINGNSEKKQAAQSFINFL
EECCCEEEEECCCCCCCEEEEEECCHHHHCEEECCEEEEECCCCCCHHHHHHHHHHHHHH
SKPENAVRNMSYIGYTSVISGGDSDVVFDYVKWNYGADESDTDVVDYPLGYFFSGDSDDE
CCCHHHHHCCHHCCHHHEECCCCCCEEEEEEEECCCCCCCCCCEEECCCCEEECCCCCCC
RYILKVPEEQTYRQLSAQYPTQEVMDRSAIMQYFDAEETTRINQMWINVRCYNIKNVPVW
EEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCEEEEEEEEEEEEEECCCCHH
IWVLAGIIVVTLIALSVKLKINKKYD
HHHHHHHHHHHHHHHHEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]