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The map label for this gene is lapT [H]

Identifier: 238921542

GI number: 238921542

Start: 3583331

End: 3583969

Strand: Reverse

Name: lapT [H]

Synonym: NT01EI_3694

Alternate gene names: 238921542

Gene position: 3583969-3583331 (Counterclockwise)

Preceding gene: 238921543

Following gene: 238921541

Centisome position: 94.01

GC content: 38.34

Gene sequence:

>639_bases
ATGTTGTCAAAATCACCTTCAGGTAAGATTGAAGGGGTTGAGATGGATATTTTTAATGCGTTTTGTAAAAAGAATGATTG
TACGCTGAGGGTCAAGGAATACACCTTTGAAGGGATGTTGGGGGCCTTAGCCAGCGGTCAGGCCGATGTAGCCTTTTCAG
CAATATCCATTACGCCAAAACGTGAAGCTGTGATGGACTTCTCCAAGCCATACTATGAGAACTCTTGGAATTTGGTAAGC
ATGACCAGCAGAAATATTAAGATAACTGACTTATCACAACTCAAAAAATATAAAATTGGTTATCCGAGAGGCATGGCCTA
CTCAGGTCTGATTAAGAGCAAGCTGCAACCAAAAGGTTATTACCAGCTGAGCGACGTGAGGCTTTATCCTTCCTATAGTG
AAACCATGCAAGACCTGAAAAATGGTAATATTGACCTTGCTTTTGTTGAAGACCCAGTATTAATCTTTTATAAAAATAAG
AAAAATTATCCAATCATCAATAGCTATAGCTTTAAGAGGATGGATTCTCTTGGGTTTGCTTTTAAAAAGAACTCCCCTCT
ACGTAACCAGTTTAACCAGTTTCTGGATGAAGAAGGACAGGAAAAAATAAATGTTATTGTTGATAAATGGATGAAGTAG

Upstream 100 bases:

>100_bases
CAAAGCATTACTGAGCACTGTTGTAGTATTTGGCATTCTCTCATCACCAGCAATAGCGACTACAGAGCTAAATGTTGCTG
TTGCGGCTAATTCCCCGCCG

Downstream 100 bases:

>100_bases
CGTCAGGTGCCACATGAACTGGACTGAACTGGTTAATGTTGTTTTACAACTGTCACAGGGAGTCATCACTACGACTCTAG
TTACGGTCAGCTGTTTTATT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLSKSPSGKIEGVEMDIFNAFCKKNDCTLRVKEYTFEGMLGALASGQADVAFSAISITPKREAVMDFSKPYYENSWNLVS
MTSRNIKITDLSQLKKYKIGYPRGMAYSGLIKSKLQPKGYYQLSDVRLYPSYSETMQDLKNGNIDLAFVEDPVLIFYKNK
KNYPIINSYSFKRMDSLGFAFKKNSPLRNQFNQFLDEEGQEKINVIVDKWMK

Sequences:

>Translated_212_residues
MLSKSPSGKIEGVEMDIFNAFCKKNDCTLRVKEYTFEGMLGALASGQADVAFSAISITPKREAVMDFSKPYYENSWNLVS
MTSRNIKITDLSQLKKYKIGYPRGMAYSGLIKSKLQPKGYYQLSDVRLYPSYSETMQDLKNGNIDLAFVEDPVLIFYKNK
KNYPIINSYSFKRMDSLGFAFKKNSPLRNQFNQFLDEEGQEKINVIVDKWMK
>Mature_212_residues
MLSKSPSGKIEGVEMDIFNAFCKKNDCTLRVKEYTFEGMLGALASGQADVAFSAISITPKREAVMDFSKPYYENSWNLVS
MTSRNIKITDLSQLKKYKIGYPRGMAYSGLIKSKLQPKGYYQLSDVRLYPSYSETMQDLKNGNIDLAFVEDPVLIFYKNK
KNYPIINSYSFKRMDSLGFAFKKNSPLRNQFNQFLDEEGQEKINVIVDKWMK

Specific function: Binds arginine; part of the arginine periplasmic transport system [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=212, Percent_Identity=27.3584905660377, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788228, Length=210, Percent_Identity=25.2380952380952, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1787085, Length=148, Percent_Identity=28.3783783783784, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 24375; Mature: 24375

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKSPSGKIEGVEMDIFNAFCKKNDCTLRVKEYTFEGMLGALASGQADVAFSAISITPK
CCCCCCCCCEEEEEHHHHHHHHCCCCCEEEEEEEEHHHHHHHHCCCCCCEEEEEEEECCC
REAVMDFSKPYYENSWNLVSMTSRNIKITDLSQLKKYKIGYPRGMAYSGLIKSKLQPKGY
HHHHHHCCCCCCCCCCCEEEECCCCEEEEEHHHHHHHCCCCCCCCCHHHHHHHCCCCCCC
YQLSDVRLYPSYSETMQDLKNGNIDLAFVEDPVLIFYKNKKNYPIINSYSFKRMDSLGFA
EEEEEEEECCCHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCEECCCCHHHHHHHCEE
FKKNSPLRNQFNQFLDEEGQEKINVIVDKWMK
EECCCCHHHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLSKSPSGKIEGVEMDIFNAFCKKNDCTLRVKEYTFEGMLGALASGQADVAFSAISITPK
CCCCCCCCCEEEEEHHHHHHHHCCCCCEEEEEEEEHHHHHHHHCCCCCCEEEEEEEECCC
REAVMDFSKPYYENSWNLVSMTSRNIKITDLSQLKKYKIGYPRGMAYSGLIKSKLQPKGY
HHHHHHCCCCCCCCCCCEEEECCCCEEEEEHHHHHHHCCCCCCCCCHHHHHHHCCCCCCC
YQLSDVRLYPSYSETMQDLKNGNIDLAFVEDPVLIFYKNKKNYPIINSYSFKRMDSLGFA
EEEEEEEECCCHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCEECCCCHHHHHHHCEE
FKKNSPLRNQFNQFLDEEGQEKINVIVDKWMK
EECCCCHHHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8359916 [H]