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The map label for this gene is dam [H]

Identifier: 238921469

GI number: 238921469

Start: 3507702

End: 3508520

Strand: Reverse

Name: dam [H]

Synonym: NT01EI_3621

Alternate gene names: 238921469

Gene position: 3508520-3507702 (Counterclockwise)

Preceding gene: 238921470

Following gene: 238921468

Centisome position: 92.03

GC content: 56.65

Gene sequence:

>819_bases
ATGAAGAAGAAGCGCGCATTTTTGAAATGGGCCGGCGGGAAATATCCTTTGATTGAAGAGATCCGTCGGCACCTGCCGGC
GGGCGAGAGGCTGATAGAGCCTTTCGTCGGCGCTGGCTCTGTGTTTTTAAACACGGATTACGATGAGTACCTGTTGGCGG
ACATTAACGCCGATCTGGTCGGGTTGTACAATATTGTGAAAGATGAGACGGATCGCTTTATCAGCGATGCCCGTCAGATG
TTTTGCCCTGAACTGAACGTCTCGGAGGAGTATTACCGCATCCGCGAGGCCTTTAACCTGTGCGACGATCGTTACCAGCG
GGCGCTGTACTTCCTCTATCTGAACCGGCACTGCTATAACGGTCTGTGCCGCTATAATGCCAAGGGGGAGTTCAACGTCC
CCTTCGGGCGCTATAAAAAGCCCTACTTCCCGCAGGAGGAGCTGGAGTTTTTTGCCCGCAAGGCCGAGCGCGCCGTGTTT
GTCTGCGAACATTACCAGCAGACGATGCTGCGCGCGCAGCCGGGTGCCGTGGTGTATTGCGATCCACCCTACGCCCCGCT
GTCCGCTACCGCCAATTTTACCGCCTACCATGTCAACGGCTTTGGCTTTGCCGAGCAGATGCGTCTGGCGGAGTTGGCCG
GTGAACTGGCCCAGCGTCAGGTGCCGGTGCTGATCTCTAACCATGAAACCCCGCTAACGCGGGAGTGGTATGCGCAGGCC
GATGCGCTGCACGTCGTGAAAGTTCGGCGTACCATCAGCAGAAAAACTGACGGGCGCAGCAAAGTGAACGAATTATTGGC
GCTGTACGGCAAGCGCTGA

Upstream 100 bases:

>100_bases
ATGCAAAGTAAGCTGTCACCCATAAAACGCGCGCGATGTGCTGTCTGAAAAGGTACGCCCTGGCGAATTGAAAGAGTAGA
AATAACTGACTGGCGACGGC

Downstream 100 bases:

>100_bases
GCGCGGTCGTCAGCCCCCCGAGCCCAGGAGAAACGGATGAACCCGTATTTAATTGCCCCGTCGATTTTGTCCGCAGACTT
TGCCCGGCTGGGCGAAGATA

Product: retron adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.SmaII [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKKKRAFLKWAGGKYPLIEEIRRHLPAGERLIEPFVGAGSVFLNTDYDEYLLADINADLVGLYNIVKDETDRFISDARQM
FCPELNVSEEYYRIREAFNLCDDRYQRALYFLYLNRHCYNGLCRYNAKGEFNVPFGRYKKPYFPQEELEFFARKAERAVF
VCEHYQQTMLRAQPGAVVYCDPPYAPLSATANFTAYHVNGFGFAEQMRLAELAGELAQRQVPVLISNHETPLTREWYAQA
DALHVVKVRRTISRKTDGRSKVNELLALYGKR

Sequences:

>Translated_272_residues
MKKKRAFLKWAGGKYPLIEEIRRHLPAGERLIEPFVGAGSVFLNTDYDEYLLADINADLVGLYNIVKDETDRFISDARQM
FCPELNVSEEYYRIREAFNLCDDRYQRALYFLYLNRHCYNGLCRYNAKGEFNVPFGRYKKPYFPQEELEFFARKAERAVF
VCEHYQQTMLRAQPGAVVYCDPPYAPLSATANFTAYHVNGFGFAEQMRLAELAGELAQRQVPVLISNHETPLTREWYAQA
DALHVVKVRRTISRKTDGRSKVNELLALYGKR
>Mature_272_residues
MKKKRAFLKWAGGKYPLIEEIRRHLPAGERLIEPFVGAGSVFLNTDYDEYLLADINADLVGLYNIVKDETDRFISDARQM
FCPELNVSEEYYRIREAFNLCDDRYQRALYFLYLNRHCYNGLCRYNAKGEFNVPFGRYKKPYFPQEELEFFARKAERAVF
VCEHYQQTMLRAQPGAVVYCDPPYAPLSATANFTAYHVNGFGFAEQMRLAELAGELAQRQVPVLISNHETPLTREWYAQA
DALHVVKVRRTISRKTDGRSKVNELLALYGKR

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=269, Percent_Identity=66.1710037174721, Blast_Score=374, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31657; Mature: 31657

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKRAFLKWAGGKYPLIEEIRRHLPAGERLIEPFVGAGSVFLNTDYDEYLLADINADLV
CCCCCCHHEECCCCCCHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCEEEEECCCHHH
GLYNIVKDETDRFISDARQMFCPELNVSEEYYRIREAFNLCDDRYQRALYFLYLNRHCYN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLCRYNAKGEFNVPFGRYKKPYFPQEELEFFARKAERAVFVCEHYQQTMLRAQPGAVVYC
CCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DPPYAPLSATANFTAYHVNGFGFAEQMRLAELAGELAQRQVPVLISNHETPLTREWYAQA
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
DALHVVKVRRTISRKTDGRSKVNELLALYGKR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKRAFLKWAGGKYPLIEEIRRHLPAGERLIEPFVGAGSVFLNTDYDEYLLADINADLV
CCCCCCHHEECCCCCCHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCEEEEECCCHHH
GLYNIVKDETDRFISDARQMFCPELNVSEEYYRIREAFNLCDDRYQRALYFLYLNRHCYN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLCRYNAKGEFNVPFGRYKKPYFPQEELEFFARKAERAVFVCEHYQQTMLRAQPGAVVYC
CCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DPPYAPLSATANFTAYHVNGFGFAEQMRLAELAGELAQRQVPVLISNHETPLTREWYAQA
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHH
DALHVVKVRRTISRKTDGRSKVNELLALYGKR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10383952 [H]