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The map label for this gene is hyaD [H]

Identifier: 238921263

GI number: 238921263

Start: 3291855

End: 3292448

Strand: Reverse

Name: hyaD [H]

Synonym: NT01EI_3406

Alternate gene names: 238921263

Gene position: 3292448-3291855 (Counterclockwise)

Preceding gene: 238921264

Following gene: 238921262

Centisome position: 86.36

GC content: 65.99

Gene sequence:

>594_bases
ATGAGTGAGCAGCGCGTGGTGGTGATGGGGCTGGGCAACCTGCTGTGGGCCGATGAGGGCTTCGGCGTACGGGTGGCCGA
ACGGCTGTATGCCCATTACCATTGGCCGGATCAGGTGGAGATCGTCGACGGCGGCACCCAGGGGCTGAATCTGCTGGGCT
ATATTGAAAGCGCCAGCCACCTGCTGATCCTGGATGCCATCGACTACGGTCTGGCGCCGGGTACCCTGCGCACCTACGCC
GGAGAGATGGTGCCGGCCTACCTCAGCGCCAAGAAGATGAGCCTGCACCAGAGCAGCTTTTCCGAGGTGCTGGCCCTGGC
AGAGATTCGTGGCCATATGCCGTCCCGCGTGGCGCTGGTGGGGCTACAGCCCGCGCGGCTGGATGAGTATGGCGGCAGCC
TGACCGGGGTGGCGCGGGCCCAGCTGCCCGCGGCGGAGCAGGCGGCACTGGCGCAGCTGGCCGACTGGGGGATCGTCCCG
CAGCCGAGCGAGGAGCGCCGCTGCCTGAACTACGAATGTCTGTCGATGGAGAACTATGAGGGCGTCCGCATTCGCCAGTA
TCGGATGACGCTGGAGGGACAGGAGGAGTGTTGA

Upstream 100 bases:

>100_bases
TCCGCGAAGATATCATGTCTGACGACACGGTTATCTCTACCATGATCAATGGATTCCGCAGCCATAAGTTCCCGAAGCGC
AAACGCGACAAGGAGGCGTC

Downstream 100 bases:

>100_bases
ACGACAATCCGTTTTCCGCGCTGTGGCAGCGCATGCTGGCGCGCGGCTGGCAGCCGGTAGAGGCGCATTCGCTGGACGGC
TGGCTGGCGCAGGCGCCGGA

Product: hydrogenase 1 maturation protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDQVEIVDGGTQGLNLLGYIESASHLLILDAIDYGLAPGTLRTYA
GEMVPAYLSAKKMSLHQSSFSEVLALAEIRGHMPSRVALVGLQPARLDEYGGSLTGVARAQLPAAEQAALAQLADWGIVP
QPSEERRCLNYECLSMENYEGVRIRQYRMTLEGQEEC

Sequences:

>Translated_197_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDQVEIVDGGTQGLNLLGYIESASHLLILDAIDYGLAPGTLRTYA
GEMVPAYLSAKKMSLHQSSFSEVLALAEIRGHMPSRVALVGLQPARLDEYGGSLTGVARAQLPAAEQAALAQLADWGIVP
QPSEERRCLNYECLSMENYEGVRIRQYRMTLEGQEEC
>Mature_196_residues
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDQVEIVDGGTQGLNLLGYIESASHLLILDAIDYGLAPGTLRTYAG
EMVPAYLSAKKMSLHQSSFSEVLALAEIRGHMPSRVALVGLQPARLDEYGGSLTGVARAQLPAAEQAALAQLADWGIVPQ
PSEERRCLNYECLSMENYEGVRIRQYRMTLEGQEEC

Specific function: Protease involved in the C-terminal processing of hyaB, the large subunit of hydrogenase 1 (Potential) [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=194, Percent_Identity=86.5979381443299, Blast_Score=326, Evalue=7e-91,
Organism=Escherichia coli, GI1789367, Length=161, Percent_Identity=40.3726708074534, Blast_Score=108, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 21725; Mature: 21594

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDQVEIVDGGTQGLNLLGYIESASH
CCCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCE
LLILDAIDYGLAPGTLRTYAGEMVPAYLSAKKMSLHQSSFSEVLALAEIRGHMPSRVALV
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
GLQPARLDEYGGSLTGVARAQLPAAEQAALAQLADWGIVPQPSEERRCLNYECLSMENYE
ECCCHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCC
GVRIRQYRMTLEGQEEC
CEEEEEEEEEECCCCCC
>Mature Secondary Structure 
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDQVEIVDGGTQGLNLLGYIESASH
CCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCE
LLILDAIDYGLAPGTLRTYAGEMVPAYLSAKKMSLHQSSFSEVLALAEIRGHMPSRVALV
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
GLQPARLDEYGGSLTGVARAQLPAAEQAALAQLADWGIVPQPSEERRCLNYECLSMENYE
ECCCHHHHHCCCCEEEHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCC
GVRIRQYRMTLEGQEEC
CEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]