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The map label for this gene is iciA

Identifier: 238921220

GI number: 238921220

Start: 3249457

End: 3250350

Strand: Direct

Name: iciA

Synonym: NT01EI_3363

Alternate gene names: 238921220

Gene position: 3249457-3250350 (Clockwise)

Preceding gene: 238921217

Following gene: 238921228

Centisome position: 85.24

GC content: 63.65

Gene sequence:

>894_bases
ATGAAACGCCCGGACTACCGGACCTTGCAGGCACTGGACGCCGTCATCCGCGAACGTGGTTTTGAGCGCGCTGCTCAAAA
ACTTTGCATCACGCAGTCGGCGGTCTCCCAACGCATCAAGCAACTGGAAAACCTGTTTGGCCAGCCGCTGCTGGTACGTA
CCGTGCCGCCGCGCCCTACCGAGCAGGGACAGCGCCTGCTGGCGCTGCTGCACCAGGTCGAACTGCTGGAGGAGGAGTGG
CTGGGTAATGACAACAGCAGCGATGGTCCGCTGCTACTGTCGCTGGCAGTCAACGCCGACAGTCTGGCGACCTGGCTGCT
GCCGGCCCTGAAGCCAGTGCTGGCCGACTCACCGCTGCGCCTGAATCTGCAGGTCGAAGACGAAAGCCGCACCCAGGAGC
GCCTGCGCCGCGGTGAAGTGGTCGGCGCAGTGAGTCTGCAGCCCCAGCCGCTGCCGAGCTGCCTGGTGGATCGTCTGGGC
GCGCTGGACTATCTGTTCTGCGCCTCCCCCGCCTTTGCCGCCCGCTACTTCCCCAACGGGGTAACGCGCTCATCGTTACT
GAAAGCGCCAGCGGTCGCCTTTGACCACCTGGACGACATGCATCAGGCTTTCCTACAGCAAAACTTCGAACTGCCGCCGG
GCAGCGTACCCTGTCATATCGTTAACTCATCCGAAGCGTTTGTACAGCTGGCGCTGCAGGGCACTACCTGCTGTATGCTG
CCGCACCTGCAGGTTGAGCGCGAGCTGCGCAAAGGAGTACTGATCGATCTGACGCCGGGCATGGTCCAGCGCCGAATGCT
GTACTGGCACCGTTTTGCCCCGGAGAGCCGCCTGATGCGGCGGGTCACCGATGCGCTGATAAGCCACGGCCGCGACGTGC
TGCGCCAGGACTAG

Upstream 100 bases:

>100_bases
TAGGGCCCTCCGCCTAGGGCGGACCATGCGCGCCGTATTCCCGACGTGAGCAATAACGTTTATACATAACAAAAATGGAA
CCAAGGGGACAGATACCATA

Downstream 100 bases:

>100_bases
ACACTGGAGCTGCGCGCGGCAACGCGGGATCGGCAATCGATCCCGCCCACTATCGGCCCGTTATTTACTCAGGTTAAACA
CCACCTCAACCCGATCGTCG

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPTEQGQRLLALLHQVELLEEEW
LGNDNSSDGPLLLSLAVNADSLATWLLPALKPVLADSPLRLNLQVEDESRTQERLRRGEVVGAVSLQPQPLPSCLVDRLG
ALDYLFCASPAFAARYFPNGVTRSSLLKAPAVAFDHLDDMHQAFLQQNFELPPGSVPCHIVNSSEAFVQLALQGTTCCML
PHLQVERELRKGVLIDLTPGMVQRRMLYWHRFAPESRLMRRVTDALISHGRDVLRQD

Sequences:

>Translated_297_residues
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPTEQGQRLLALLHQVELLEEEW
LGNDNSSDGPLLLSLAVNADSLATWLLPALKPVLADSPLRLNLQVEDESRTQERLRRGEVVGAVSLQPQPLPSCLVDRLG
ALDYLFCASPAFAARYFPNGVTRSSLLKAPAVAFDHLDDMHQAFLQQNFELPPGSVPCHIVNSSEAFVQLALQGTTCCML
PHLQVERELRKGVLIDLTPGMVQRRMLYWHRFAPESRLMRRVTDALISHGRDVLRQD
>Mature_297_residues
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPTEQGQRLLALLHQVELLEEEW
LGNDNSSDGPLLLSLAVNADSLATWLLPALKPVLADSPLRLNLQVEDESRTQERLRRGEVVGAVSLQPQPLPSCLVDRLG
ALDYLFCASPAFAARYFPNGVTRSSLLKAPAVAFDHLDDMHQAFLQQNFELPPGSVPCHIVNSSEAFVQLALQGTTCCML
PHLQVERELRKGVLIDLTPGMVQRRMLYWHRFAPESRLMRRVTDALISHGRDVLRQD

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789283, Length=297, Percent_Identity=85.8585858585859, Blast_Score=530, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ICIA_EDWI9 (O52399)

Other databases:

- EMBL:   AF037440
- EMBL:   CP001600
- RefSeq:   YP_002934735.1
- ProteinModelPortal:   O52399
- GeneID:   7959662
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3363
- OMA:   YLFVASP
- ProtClustDB:   PRK03635
- HAMAP:   MF_00513
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039
- TIGRFAMs:   TIGR03298

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 33393; Mature: 33393

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
EQGQRLLALLHQVELLEEEWLGNDNSSDGPLLLSLAVNADSLATWLLPALKPVLADSPLR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEE
LNLQVEDESRTQERLRRGEVVGAVSLQPQPLPSCLVDRLGALDYLFCASPAFAARYFPNG
EEEEECCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCC
VTRSSLLKAPAVAFDHLDDMHQAFLQQNFELPPGSVPCHIVNSSEAFVQLALQGTTCCML
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHEEEEECCCEEEEC
PHLQVERELRKGVLIDLTPGMVQRRMLYWHRFAPESRLMRRVTDALISHGRDVLRQD
CHHHHHHHHHCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCC
>Mature Secondary Structure
MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENLFGQPLLVRTVPPRPT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
EQGQRLLALLHQVELLEEEWLGNDNSSDGPLLLSLAVNADSLATWLLPALKPVLADSPLR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEE
LNLQVEDESRTQERLRRGEVVGAVSLQPQPLPSCLVDRLGALDYLFCASPAFAARYFPNG
EEEEECCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCC
VTRSSLLKAPAVAFDHLDDMHQAFLQQNFELPPGSVPCHIVNSSEAFVQLALQGTTCCML
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHEEEEECCCEEEEC
PHLQVERELRKGVLIDLTPGMVQRRMLYWHRFAPESRLMRRVTDALISHGRDVLRQD
CHHHHHHHHHCCEEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12542086