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The map label for this gene is ydiB [C]

Identifier: 238921205

GI number: 238921205

Start: 3230992

End: 3231780

Strand: Reverse

Name: ydiB [C]

Synonym: NT01EI_3347

Alternate gene names: 238921205

Gene position: 3231780-3230992 (Counterclockwise)

Preceding gene: 238921206

Following gene: 238921203

Centisome position: 84.77

GC content: 61.72

Gene sequence:

>789_bases
ATGTCACTGGCGGCACGCCCGGGTAACTTCGGCACCCGTTTTCATAACTATCTGTATGACGCGCTGGATCTGGATTATCT
GTATAAGGCCTTTACCACCCGCGATATTATCGCCGCCATCGGCGGCGTCAGGGCGCTGGGTATCCGCGGCTGCGCTATCT
CCATGCCGTTTAAAGAGGCGGTGATCCCGCTGGTGGATGAGATGGATGCCTCGGCAGCCGGGATCGGATCGGTGAATACC
CTCGTTAATACGGATGGCTGGTTGAAGGCCTATAACACCGACTATATCGCCATTGCCCAGCTGTTGCGTCAGTATGCACT
CGATCCCGCCATGACCTTTGCCCTGCACGGCAGCGGCGGCATGGCCAAGGCGGTCGCCTGCGCGCTGCGGGACGCGGGGT
TTACCCGTGGCTATATCGTGGCGAAAAATGCCGAGTCCGGTCAGCATCTGGCCGCGCAGTATGGCTATACCTGGTGCCGA
GAGCTGCCGGTGCAGACGCCCGTCGATCTGCTGATCAACGCCACGCCGGTGGGGATGCTCGGCGGTGAGTTTGCCGGCGC
GCTGTCATTTCATCCTGCGCGTATCGTGCAGGCCCACGCGGTATTCGAGGTGGTGGCCATACCAATAGCGACGCCGCTGG
TTCAGGCGGCACGGGCAGCGGGTAAAACGGTGATCACCGGGGCGGAGGTGTTTGCCATTCAGGCGGTGGAGCAGTTTGTC
CTGTATACCGGCATTCGCCCGGAGCATGGACTGTTTTTGCAGGCCGCGGCCCACGCCCGGGGCGAGTGA

Upstream 100 bases:

>100_bases
CCGTGTTTTGCGCCATGATGTCGGGACTGTGCGGCGAACTTTTCTGGCCTAAGGAGCTATCATCATGACCCGACACCTCA
GCAAGGATACCCAACTGTGC

Downstream 100 bases:

>100_bases
CGCCCGACCACGGACGTCGCTGTGGCGGGCGTGGCGGGAATCACTCCTGCTGATCGGTGCTATAGGGCAGGGGCGACAGT
GTCAGCTGGCTCCGCGCGTC

Product: hypothetical protein

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSLAARPGNFGTRFHNYLYDALDLDYLYKAFTTRDIIAAIGGVRALGIRGCAISMPFKEAVIPLVDEMDASAAGIGSVNT
LVNTDGWLKAYNTDYIAIAQLLRQYALDPAMTFALHGSGGMAKAVACALRDAGFTRGYIVAKNAESGQHLAAQYGYTWCR
ELPVQTPVDLLINATPVGMLGGEFAGALSFHPARIVQAHAVFEVVAIPIATPLVQAARAAGKTVITGAEVFAIQAVEQFV
LYTGIRPEHGLFLQAAAHARGE

Sequences:

>Translated_262_residues
MSLAARPGNFGTRFHNYLYDALDLDYLYKAFTTRDIIAAIGGVRALGIRGCAISMPFKEAVIPLVDEMDASAAGIGSVNT
LVNTDGWLKAYNTDYIAIAQLLRQYALDPAMTFALHGSGGMAKAVACALRDAGFTRGYIVAKNAESGQHLAAQYGYTWCR
ELPVQTPVDLLINATPVGMLGGEFAGALSFHPARIVQAHAVFEVVAIPIATPLVQAARAAGKTVITGAEVFAIQAVEQFV
LYTGIRPEHGLFLQAAAHARGE
>Mature_261_residues
SLAARPGNFGTRFHNYLYDALDLDYLYKAFTTRDIIAAIGGVRALGIRGCAISMPFKEAVIPLVDEMDASAAGIGSVNTL
VNTDGWLKAYNTDYIAIAQLLRQYALDPAMTFALHGSGGMAKAVACALRDAGFTRGYIVAKNAESGQHLAAQYGYTWCRE
LPVQTPVDLLINATPVGMLGGEFAGALSFHPARIVQAHAVFEVVAIPIATPLVQAARAAGKTVITGAEVFAIQAVEQFVL
YTGIRPEHGLFLQAAAHARGE

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787983, Length=259, Percent_Identity=28.957528957529, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 27858; Mature: 27727

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAARPGNFGTRFHNYLYDALDLDYLYKAFTTRDIIAAIGGVRALGIRGCAISMPFKEA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCEEEECCHHHH
VIPLVDEMDASAAGIGSVNTLVNTDGWLKAYNTDYIAIAQLLRQYALDPAMTFALHGSGG
HHHHHHHCCCCCCCCCCCHHEECCCCCEEECCCCHHHHHHHHHHHCCCCHHEEEEECCCC
MAKAVACALRDAGFTRGYIVAKNAESGQHLAAQYGYTWCRELPVQTPVDLLINATPVGML
HHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHCHHHHHHCCCCCCHHHEEECCCCCCC
GGEFAGALSFHPARIVQAHAVFEVVAIPIATPLVQAARAAGKTVITGAEVFAIQAVEQFV
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
LYTGIRPEHGLFLQAAAHARGE
HHCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SLAARPGNFGTRFHNYLYDALDLDYLYKAFTTRDIIAAIGGVRALGIRGCAISMPFKEA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCEEEECCHHHH
VIPLVDEMDASAAGIGSVNTLVNTDGWLKAYNTDYIAIAQLLRQYALDPAMTFALHGSGG
HHHHHHHCCCCCCCCCCCHHEECCCCCEEECCCCHHHHHHHHHHHCCCCHHEEEEECCCC
MAKAVACALRDAGFTRGYIVAKNAESGQHLAAQYGYTWCRELPVQTPVDLLINATPVGML
HHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHCHHHHHHCCCCCCHHHEEECCCCCCC
GGEFAGALSFHPARIVQAHAVFEVVAIPIATPLVQAARAAGKTVITGAEVFAIQAVEQFV
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
LYTGIRPEHGLFLQAAAHARGE
HHCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]