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The map label for this gene is hydN [H]

Identifier: 238921139

GI number: 238921139

Start: 3158799

End: 3159341

Strand: Direct

Name: hydN [H]

Synonym: NT01EI_3280

Alternate gene names: 238921139

Gene position: 3158799-3159341 (Clockwise)

Preceding gene: 238921138

Following gene: 238921140

Centisome position: 82.86

GC content: 60.59

Gene sequence:

>543_bases
ATGAATCGGTTTGTCATTGCGGATCCCAAAACATGCATCGGCTGCCGTACCTGCGAAATCGCCTGCGTCATGGCCCACCG
GGAGGAGAGCGAGCTGCATAACCTCAGCCGCGATAACTTCACTCCGCGCATTCATGTCATTAAGGGAGACAACATCAGCA
CGGCGATCCTGTGCCGCCAGTGTGAAGACGCCCCCTGTGCCAATGTCTGCCCCAACGGTGCCATCAGCCGCCAGCAGGAC
TTCATCTGCGTTGACCAGGAAAAATGCATCGGCTGCAAAACCTGCGTGGTCGCCTGTCCTTACGGCACCATGGAGGTCGT
CACCACCACGGTGACGCAGCGTTACGGCAGCAGCGGCTGCATCAGCAGCAGCAAGGCTGAGGCCAACAAGTGCGATCTGT
GCAATCACCGCGCCAACGGCCCGGCCTGCGTGGAGGCCTGCCCGACCAAGGCCCTGAAGCTTATCGATCGCAACGCCATG
CAGGCGCTGATCCAGGAGCGACGCCAGCGCGCCGCTTTCGATAACCTGGGCGATCTGCTGTAA

Upstream 100 bases:

>100_bases
CATGCCGCGTATCACTTTTGATAACCCTCCGGCGGGGCAGCAACAGCGGCTCCAGCGGGGTTCAACGGCGCGACACTATA
ACGATATCGGGAGTAATTGA

Downstream 100 bases:

>100_bases
ACGGGCGTATGCCCCTCTCCAGCGTTCCCGCGGGGGTCTCCCCCGCCACCGGCGCACCGCGGCAAAGCGAGAGCCAACGC
TCAGATATGACATGCCGCGT

Product: 4Fe-4S iron-sulfur binding domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MNRFVIADPKTCIGCRTCEIACVMAHREESELHNLSRDNFTPRIHVIKGDNISTAILCRQCEDAPCANVCPNGAISRQQD
FICVDQEKCIGCKTCVVACPYGTMEVVTTTVTQRYGSSGCISSSKAEANKCDLCNHRANGPACVEACPTKALKLIDRNAM
QALIQERRQRAAFDNLGDLL

Sequences:

>Translated_180_residues
MNRFVIADPKTCIGCRTCEIACVMAHREESELHNLSRDNFTPRIHVIKGDNISTAILCRQCEDAPCANVCPNGAISRQQD
FICVDQEKCIGCKTCVVACPYGTMEVVTTTVTQRYGSSGCISSSKAEANKCDLCNHRANGPACVEACPTKALKLIDRNAM
QALIQERRQRAAFDNLGDLL
>Mature_180_residues
MNRFVIADPKTCIGCRTCEIACVMAHREESELHNLSRDNFTPRIHVIKGDNISTAILCRQCEDAPCANVCPNGAISRQQD
FICVDQEKCIGCKTCVVACPYGTMEVVTTTVTQRYGSSGCISSSKAEANKCDLCNHRANGPACVEACPTKALKLIDRNAM
QALIQERRQRAAFDNLGDLL

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=173, Percent_Identity=63.5838150289017, Blast_Score=230, Evalue=4e-62,
Organism=Escherichia coli, GI1788811, Length=172, Percent_Identity=54.0697674418605, Blast_Score=181, Evalue=2e-47,
Organism=Escherichia coli, GI2367245, Length=161, Percent_Identity=55.9006211180124, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI87082180, Length=173, Percent_Identity=41.0404624277457, Blast_Score=143, Evalue=5e-36,
Organism=Escherichia coli, GI87082179, Length=167, Percent_Identity=40.7185628742515, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1789079, Length=198, Percent_Identity=36.8686868686869, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI87082114, Length=202, Percent_Identity=35.1485148514851, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1787872, Length=184, Percent_Identity=30.9782608695652, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1787122, Length=168, Percent_Identity=30.952380952381, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI226510944, Length=175, Percent_Identity=33.7142857142857, Blast_Score=73, Evalue=9e-15,
Organism=Escherichia coli, GI2367345, Length=176, Percent_Identity=31.25, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1787963, Length=163, Percent_Identity=33.7423312883436, Blast_Score=70, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 19703; Mature: 19703

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

10.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
12.2 %Cys+Met (Translated Protein)
10.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
12.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFVIADPKTCIGCRTCEIACVMAHREESELHNLSRDNFTPRIHVIKGDNISTAILCRQ
CCCEEECCCCHHHCCCHHHEEEEEHHCCHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHH
CEDAPCANVCPNGAISRQQDFICVDQEKCIGCKTCVVACPYGTMEVVTTTVTQRYGSSGC
CCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCC
ISSSKAEANKCDLCNHRANGPACVEACPTKALKLIDRNAMQALIQERRQRAAFDNLGDLL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRFVIADPKTCIGCRTCEIACVMAHREESELHNLSRDNFTPRIHVIKGDNISTAILCRQ
CCCEEECCCCHHHCCCHHHEEEEEHHCCHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHH
CEDAPCANVCPNGAISRQQDFICVDQEKCIGCKTCVVACPYGTMEVVTTTVTQRYGSSGC
CCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCC
ISSSKAEANKCDLCNHRANGPACVEACPTKALKLIDRNAMQALIQERRQRAAFDNLGDLL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]