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The map label for this gene is hemL

Identifier: 238920976

GI number: 238920976

Start: 2995708

End: 2996991

Strand: Direct

Name: hemL

Synonym: NT01EI_3106

Alternate gene names: 238920976

Gene position: 2995708-2996991 (Clockwise)

Preceding gene: 238920973

Following gene: 238920977

Centisome position: 78.58

GC content: 61.99

Gene sequence:

>1284_bases
ATGAACAAGTCCGAACGACTGTATGAGCAGGCTAAAACGCTGATCCCTGGCGGCGTTAACTCACCAGTGCGCGCCTTTAC
CGGCGTTGGCGGTACACCGCTGTTTATCGAGCGGGCCGACGGCGCCCATCTGTATGATGCCGATGGCAAGGCCTACATCG
ATTACGTAGGCTCTTGGGGACCCATGATCCTGGGTCATAACCACCCGGTGATCCGCGATGCGGTGATCGCCGCTGTCGGG
CGCGGGCTAAGCTTCGGCGCGCCGACCGAGATGGAGGTGCGCATGGCTGAGCTGGTCACCTCGCTTATTGACAGTATGGA
TATGGTGCGCATGGTGAACTCCGGCACCGAAGCTACCATGAGCGCCATCCGTCTGGCGCGCGGCTTTACCGGCCGTGACA
AGCTCATCAAATTCGAAGGCTGCTATCACGGTCACGCCGACCATCTGCTGGTCAAGGCCGGCTCCGGCGCCCTGACCCTG
GGTCAGCCCAACTCCCCGGGAGTACCAGCTGATTTCGCCAAACATACCCTGACCTGCAGCTACAACGATCTGGACTCAGT
GCGCGCCGCTTTCGCCCGCTATCCGCAGGAGATCGCCTGCATCATCGTCGAGCCGGTGGCAGGCAACATGAACTGCATTC
CGCCCCAGCCGGGCTTCCTGCAAGGACTGCGTGAACTGTGCGACCAGTACGGCGCACTGCTGATCATCGACGAGGTGATG
ACCGGATTCCGTGTCGCCCTGGGCGGTGCCCAGGAGTACTACGACGTCACCCCGGATCTGACCTGCCTGGGCAAGATCAT
CGGCGGCGGCATGCCGGTGGGCGCCTTCGGCGGACGCCGCGAGGTGATGGAGGCGCTGGCGCCGACCGGCCCGATCTACC
AAGCGGGAACGCTGTCCGGTAACCCGGTCGCCATGGCCGCCGGCTACGCCTGCCTGAATGAGATCAACCAACCGGGGATC
TATCCACAGCTGGCAGAGCTGACCGATAATCTGGCCGAAGGGCTGCTGGCAGCCGCACGCGAAGAGAAGATCCCACTGGT
GGTGAACCACGTCGGCGGCATGTTCGGCATCTTCTTCACCGACCAGCCTAGCGTCACCTGTTACCAGGATGTGCTGCGCT
GTGACGCCGAGCGCTTTAAGCGCTTCTTCCACCTGATGCTGGAACAGGGTATCTATCTGGCGCCGTCCGCGTTCGAAGCG
GGCTTTATGTCCCTGGCCCACAGTCAGGAAGATATTCAAAAAACCATCGACGCCGCCCGCTGCAGCTTCGCCAAGCTGAA
GTAA

Upstream 100 bases:

>100_bases
CCATCGTTATAAGGCACAACGGCGCATTTCATCCGACGAGAGACTTCTTTAAGATAGCACGGGATTATTACCTTAATTTT
CTACACGTCTGGAGCCGATA

Downstream 100 bases:

>100_bases
CACCCACAGAATCCTCTGCGGAGAACGTTGACGGGGCTGACCACGCGAGCCGCCAAACGCGTAGCCATCCCCGCACAGGG
TACTCCGCCGACGGCGCGCG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNKSERLYEQAKTLIPGGVNSPVRAFTGVGGTPLFIERADGAHLYDADGKAYIDYVGSWGPMILGHNHPVIRDAVIAAVG
RGLSFGAPTEMEVRMAELVTSLIDSMDMVRMVNSGTEATMSAIRLARGFTGRDKLIKFEGCYHGHADHLLVKAGSGALTL
GQPNSPGVPADFAKHTLTCSYNDLDSVRAAFARYPQEIACIIVEPVAGNMNCIPPQPGFLQGLRELCDQYGALLIIDEVM
TGFRVALGGAQEYYDVTPDLTCLGKIIGGGMPVGAFGGRREVMEALAPTGPIYQAGTLSGNPVAMAAGYACLNEINQPGI
YPQLAELTDNLAEGLLAAAREEKIPLVVNHVGGMFGIFFTDQPSVTCYQDVLRCDAERFKRFFHLMLEQGIYLAPSAFEA
GFMSLAHSQEDIQKTIDAARCSFAKLK

Sequences:

>Translated_427_residues
MNKSERLYEQAKTLIPGGVNSPVRAFTGVGGTPLFIERADGAHLYDADGKAYIDYVGSWGPMILGHNHPVIRDAVIAAVG
RGLSFGAPTEMEVRMAELVTSLIDSMDMVRMVNSGTEATMSAIRLARGFTGRDKLIKFEGCYHGHADHLLVKAGSGALTL
GQPNSPGVPADFAKHTLTCSYNDLDSVRAAFARYPQEIACIIVEPVAGNMNCIPPQPGFLQGLRELCDQYGALLIIDEVM
TGFRVALGGAQEYYDVTPDLTCLGKIIGGGMPVGAFGGRREVMEALAPTGPIYQAGTLSGNPVAMAAGYACLNEINQPGI
YPQLAELTDNLAEGLLAAAREEKIPLVVNHVGGMFGIFFTDQPSVTCYQDVLRCDAERFKRFFHLMLEQGIYLAPSAFEA
GFMSLAHSQEDIQKTIDAARCSFAKLK
>Mature_427_residues
MNKSERLYEQAKTLIPGGVNSPVRAFTGVGGTPLFIERADGAHLYDADGKAYIDYVGSWGPMILGHNHPVIRDAVIAAVG
RGLSFGAPTEMEVRMAELVTSLIDSMDMVRMVNSGTEATMSAIRLARGFTGRDKLIKFEGCYHGHADHLLVKAGSGALTL
GQPNSPGVPADFAKHTLTCSYNDLDSVRAAFARYPQEIACIIVEPVAGNMNCIPPQPGFLQGLRELCDQYGALLIIDEVM
TGFRVALGGAQEYYDVTPDLTCLGKIIGGGMPVGAFGGRREVMEALAPTGPIYQAGTLSGNPVAMAAGYACLNEINQPGI
YPQLAELTDNLAEGLLAAAREEKIPLVVNHVGGMFGIFFTDQPSVTCYQDVLRCDAERFKRFFHLMLEQGIYLAPSAFEA
GFMSLAHSQEDIQKTIDAARCSFAKLK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=323, Percent_Identity=29.4117647058824, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI13994255, Length=383, Percent_Identity=26.1096605744125, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI284507298, Length=237, Percent_Identity=29.957805907173, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=83.0985915492958, Blast_Score=741, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=385, Percent_Identity=32.4675324675325, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788044, Length=332, Percent_Identity=33.433734939759, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI145693181, Length=309, Percent_Identity=31.0679611650485, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1789759, Length=313, Percent_Identity=32.9073482428115, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1787560, Length=359, Percent_Identity=30.3621169916435, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1786991, Length=384, Percent_Identity=28.125, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=312, Percent_Identity=28.525641025641, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71992977, Length=363, Percent_Identity=28.3746556473829, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI25144274, Length=221, Percent_Identity=28.5067873303167, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=339, Percent_Identity=27.4336283185841, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324432, Length=353, Percent_Identity=25.2124645892351, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356575, Length=327, Percent_Identity=29.9694189602446, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21357415, Length=287, Percent_Identity=30.3135888501742, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_EDWI9 (C5BAP9)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934491.1
- ProteinModelPortal:   C5BAP9
- GeneID:   7961875
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3106
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45865; Mature: 45865

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSERLYEQAKTLIPGGVNSPVRAFTGVGGTPLFIERADGAHLYDADGKAYIDYVGSWG
CCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEECCCCCEEECCCCCEEEEEECCCC
PMILGHNHPVIRDAVIAAVGRGLSFGAPTEMEVRMAELVTSLIDSMDMVRMVNSGTEATM
CEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SAIRLARGFTGRDKLIKFEGCYHGHADHLLVKAGSGALTLGQPNSPGVPADFAKHTLTCS
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCCHHHHHCEEEEC
YNDLDSVRAAFARYPQEIACIIVEPVAGNMNCIPPQPGFLQGLRELCDQYGALLIIDEVM
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHHH
TGFRVALGGAQEYYDVTPDLTCLGKIIGGGMPVGAFGGRREVMEALAPTGPIYQAGTLSG
HHHHHHHCCCHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPVAMAAGYACLNEINQPGIYPQLAELTDNLAEGLLAAAREEKIPLVVNHVGGMFGIFFT
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEE
DQPSVTCYQDVLRCDAERFKRFFHLMLEQGIYLAPSAFEAGFMSLAHSQEDIQKTIDAAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHH
CSFAKLK
HHHHCCC
>Mature Secondary Structure
MNKSERLYEQAKTLIPGGVNSPVRAFTGVGGTPLFIERADGAHLYDADGKAYIDYVGSWG
CCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEECCCCCEEECCCCCEEEEEECCCC
PMILGHNHPVIRDAVIAAVGRGLSFGAPTEMEVRMAELVTSLIDSMDMVRMVNSGTEATM
CEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SAIRLARGFTGRDKLIKFEGCYHGHADHLLVKAGSGALTLGQPNSPGVPADFAKHTLTCS
HHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCCHHHHHCEEEEC
YNDLDSVRAAFARYPQEIACIIVEPVAGNMNCIPPQPGFLQGLRELCDQYGALLIIDEVM
CCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHHH
TGFRVALGGAQEYYDVTPDLTCLGKIIGGGMPVGAFGGRREVMEALAPTGPIYQAGTLSG
HHHHHHHCCCHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPVAMAAGYACLNEINQPGIYPQLAELTDNLAEGLLAAAREEKIPLVVNHVGGMFGIFFT
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEEE
DQPSVTCYQDVLRCDAERFKRFFHLMLEQGIYLAPSAFEAGFMSLAHSQEDIQKTIDAAR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHH
CSFAKLK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA