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The map label for this gene is smpB

Identifier: 238920940

GI number: 238920940

Start: 2961848

End: 2962330

Strand: Direct

Name: smpB

Synonym: NT01EI_3068

Alternate gene names: 238920940

Gene position: 2961848-2962330 (Clockwise)

Preceding gene: 238920937

Following gene: 238920941

Centisome position: 77.69

GC content: 57.35

Gene sequence:

>483_bases
ATGACAAAGAAAAAAGCATACAAACCGGGTTCGGCAACCATTGCCATGAACAAGCGCGCGCGCCACGACTACTTCATTGA
AGAAGAGTTCGAGGCCGGGCTGTCGCTGCAAGGCTGGGAGGTCAAATCCCTGCGGGCCGGCAAGGCCAACATCAGTGAAA
GCTATATTTTGCTGCAAAACGGCGAGGCGTTTCTGTTTGGCGCCACCTTTACCCCGCTGAACGTGGCCTCCAGCCACGTG
GTGTGCGATCCGACGCGCACCCGCAAGCTGCTGCTCAAAGAGCGCGAGCTGGCGAGCCTGTTCGGCAGCGCCAACCGCGA
CGGCTACACCATCGTCCCGCTGTCGCTGTACTGGAAGAACGCCTGGGCCAAGCTGAAGATCGGTGTGGCTAAGGGTAAGA
AAGAGCACGACAAACGCGACGACATCAAAACCCGCGAATGGAAGCAGGACAAAGCGCGAATTATGAAAAACGCCAACCGC
TAA

Upstream 100 bases:

>100_bases
AGAGAATTGGCTTACGCACACTGGATGCCGTCCAGGGATCCGTGGCCATTCCCGGCAAAAAGTATTCATTCGCCGCTCGT
AACAGTATAATGACGCTACT

Downstream 100 bases:

>100_bases
CGCTGGCGCAGCGGCAGTAAACTCTGGTATACTGCCGCCTACACACTTGGGCCTGATTCTGGATTCGACGGGATTTGCGA
AACTCAAGGTGCATGCCGAG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTKKKAYKPGSATIAMNKRARHDYFIEEEFEAGLSLQGWEVKSLRAGKANISESYILLQNGEAFLFGATFTPLNVASSHV
VCDPTRTRKLLLKERELASLFGSANRDGYTIVPLSLYWKNAWAKLKIGVAKGKKEHDKRDDIKTREWKQDKARIMKNANR

Sequences:

>Translated_160_residues
MTKKKAYKPGSATIAMNKRARHDYFIEEEFEAGLSLQGWEVKSLRAGKANISESYILLQNGEAFLFGATFTPLNVASSHV
VCDPTRTRKLLLKERELASLFGSANRDGYTIVPLSLYWKNAWAKLKIGVAKGKKEHDKRDDIKTREWKQDKARIMKNANR
>Mature_159_residues
TKKKAYKPGSATIAMNKRARHDYFIEEEFEAGLSLQGWEVKSLRAGKANISESYILLQNGEAFLFGATFTPLNVASSHVV
CDPTRTRKLLLKERELASLFGSANRDGYTIVPLSLYWKNAWAKLKIGVAKGKKEHDKRDDIKTREWKQDKARIMKNANR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=160, Percent_Identity=81.25, Blast_Score=280, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_EDWI9 (C5BAL3)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934455.1
- GeneID:   7961839
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3068
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18219; Mature: 18088

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKKAYKPGSATIAMNKRARHDYFIEEEFEAGLSLQGWEVKSLRAGKANISESYILLQN
CCCCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCCCCCCEEECEECCCCCCCCCEEEEEC
GEAFLFGATFTPLNVASSHVVCDPTRTRKLLLKERELASLFGSANRDGYTIVPLSLYWKN
CCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECC
AWAKLKIGVAKGKKEHDKRDDIKTREWKQDKARIMKNANR
CEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKKKAYKPGSATIAMNKRARHDYFIEEEFEAGLSLQGWEVKSLRAGKANISESYILLQN
CCCCCCCCCCEEEEECCCCCCCCCCCHHHHCCCCCCCCEEECEECCCCCCCCCEEEEEC
GEAFLFGATFTPLNVASSHVVCDPTRTRKLLLKERELASLFGSANRDGYTIVPLSLYWKN
CCEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECC
AWAKLKIGVAKGKKEHDKRDDIKTREWKQDKARIMKNANR
CEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA