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The map label for this gene is ppnK

Identifier: 238920935

GI number: 238920935

Start: 2957776

End: 2958654

Strand: Direct

Name: ppnK

Synonym: NT01EI_3063

Alternate gene names: 238920935

Gene position: 2957776-2958654 (Clockwise)

Preceding gene: 238920933

Following gene: 238920936

Centisome position: 77.58

GC content: 60.41

Gene sequence:

>879_bases
ATGAACAAACGCTTTGAATCCATTGGGCTGGTCGGTCACCCGCGCCACCCTGCCGCACTGGCGACCCACGAGATGCTGTT
TCACTGGCTGACGGGGAAGGGCTACCCGGTGATCGTGGAGCGGCAGATCGCCCGCGATTTAAAGCTACAGCATGCGCTGA
CCGGCACCCTGGCCGACATCGGCCAACAGGCCGATCTGGCGGTGGTGGTCGGCGGCGACGGCAATATGCTGGGGGCGGCG
CGCATTCTGGCGCGCTATGACGTCGACGTCATCGGCGTCAACCGGGGCAACCTGGGCTTTCTGACCGATCTCGACCCGGA
CAACGCCAAGCAGCAGCTCTCCTGCGTGCTGGAGGGAGAATATAGCCGCGAACGCCGCTTTCTGCTGGAGGTTAAAGTCT
GCCGCGACGGACAGATGCACCGGCGCAGTACCGCCATCAACGAAGTGGTGCTGCATCCGGGCAAAGTGGCGCACATGATC
GAGTTTGAGGTGTACATCAACGACACCTTCGCCTTTTCCCAGCGCTCCGACGGACTGATCATCTCCACACCGACCGGCTC
CACCGCCTATTCACTCTCCGCCGGAGGCCCTATTCTGGCCCCCACCCTGGACGCCATCGCCCTGGTGCCGATGTTTCCCC
ATACCCTCTCGGCACGCCCGCTGGTGATCGACAGCAACAGCAAAATTCATCTGCGCTTCTCCCACTTCAGCAATGAGCTG
GAGATCAGCTGCGACAGCCAAATTGCGCTGCCCATTCAGCAGGGAGAGGAGGTGATCGTTCAGCGCAGCCCGTTTTATCT
GAGCCTGATCCACCCTAAGGATTACAGCTACTTCAATACCCTCAGCAGCAAGCTGGGCTGGTCAAAGAAACTGTTTTAG

Upstream 100 bases:

>100_bases
GCATAATGGGCGCCATTCTATCGCCCGCATCAGGCTGAGATCCGCCAGCGGGGCGCCAACGCAGCCCGGACGATGGTGCA
CAGCCAAGGGAAACCGGAAC

Downstream 100 bases:

>100_bases
CCGGGCATACGGCGCGGCGCGCAAATATTTGCGCCGGGCACTTTACTGGATAAAAAACCAGTTTATACTGGCATTAAATA
CAGTAGTGTGATTCCGTACA

Product: hypothetical protein

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MNKRFESIGLVGHPRHPAALATHEMLFHWLTGKGYPVIVERQIARDLKLQHALTGTLADIGQQADLAVVVGGDGNMLGAA
RILARYDVDVIGVNRGNLGFLTDLDPDNAKQQLSCVLEGEYSRERRFLLEVKVCRDGQMHRRSTAINEVVLHPGKVAHMI
EFEVYINDTFAFSQRSDGLIISTPTGSTAYSLSAGGPILAPTLDAIALVPMFPHTLSARPLVIDSNSKIHLRFSHFSNEL
EISCDSQIALPIQQGEEVIVQRSPFYLSLIHPKDYSYFNTLSSKLGWSKKLF

Sequences:

>Translated_292_residues
MNKRFESIGLVGHPRHPAALATHEMLFHWLTGKGYPVIVERQIARDLKLQHALTGTLADIGQQADLAVVVGGDGNMLGAA
RILARYDVDVIGVNRGNLGFLTDLDPDNAKQQLSCVLEGEYSRERRFLLEVKVCRDGQMHRRSTAINEVVLHPGKVAHMI
EFEVYINDTFAFSQRSDGLIISTPTGSTAYSLSAGGPILAPTLDAIALVPMFPHTLSARPLVIDSNSKIHLRFSHFSNEL
EISCDSQIALPIQQGEEVIVQRSPFYLSLIHPKDYSYFNTLSSKLGWSKKLF
>Mature_292_residues
MNKRFESIGLVGHPRHPAALATHEMLFHWLTGKGYPVIVERQIARDLKLQHALTGTLADIGQQADLAVVVGGDGNMLGAA
RILARYDVDVIGVNRGNLGFLTDLDPDNAKQQLSCVLEGEYSRERRFLLEVKVCRDGQMHRRSTAINEVVLHPGKVAHMI
EFEVYINDTFAFSQRSDGLIISTPTGSTAYSLSAGGPILAPTLDAIALVPMFPHTLSARPLVIDSNSKIHLRFSHFSNEL
EISCDSQIALPIQQGEEVIVQRSPFYLSLIHPKDYSYFNTLSSKLGWSKKLF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=259, Percent_Identity=28.1853281853282, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1788968, Length=292, Percent_Identity=77.3972602739726, Blast_Score=474, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320794, Length=242, Percent_Identity=32.2314049586777, Blast_Score=133, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6322509, Length=240, Percent_Identity=30, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325068, Length=272, Percent_Identity=30.5147058823529, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28573828, Length=307, Percent_Identity=28.9902280130293, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28573826, Length=307, Percent_Identity=28.9902280130293, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28573832, Length=307, Percent_Identity=28.9902280130293, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28573830, Length=307, Percent_Identity=28.9902280130293, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI161077047, Length=307, Percent_Identity=28.9902280130293, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20129957, Length=276, Percent_Identity=26.8115942028986, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281363321, Length=276, Percent_Identity=26.8115942028986, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281363323, Length=276, Percent_Identity=26.8115942028986, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653422, Length=276, Percent_Identity=26.8115942028986, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653424, Length=276, Percent_Identity=26.8115942028986, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_EDWI9 (C5BAK8)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934450.1
- ProteinModelPortal:   C5BAK8
- GeneID:   7961834
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3063
- OMA:   FESFMID
- ProtClustDB:   CLSK2813544
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 32334; Mature: 32334

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRFESIGLVGHPRHPAALATHEMLFHWLTGKGYPVIVERQIARDLKLQHALTGTLADI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHC
GQQADLAVVVGGDGNMLGAARILARYDVDVIGVNRGNLGFLTDLDPDNAKQQLSCVLEGE
CCCCCEEEEECCCCCEEHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCHHHEEEEEECC
YSRERRFLLEVKVCRDGQMHRRSTAINEVVLHPGKVAHMIEFEVYINDTFAFSQRSDGLI
CCCCEEEEEEEEECCCCCHHHHHHHHHHHEECCCCEEEEEEEEEEEECEEEECCCCCCEE
ISTPTGSTAYSLSAGGPILAPTLDAIALVPMFPHTLSARPLVIDSNSKIHLRFSHFSNEL
EECCCCCCEEEECCCCCEECCCHHHHEEECCCCCCCCCCEEEEECCCEEEEEEECCCCEE
EISCDSQIALPIQQGEEVIVQRSPFYLSLIHPKDYSYFNTLSSKLGWSKKLF
EEEECCEEEEECCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNKRFESIGLVGHPRHPAALATHEMLFHWLTGKGYPVIVERQIARDLKLQHALTGTLADI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHC
GQQADLAVVVGGDGNMLGAARILARYDVDVIGVNRGNLGFLTDLDPDNAKQQLSCVLEGE
CCCCCEEEEECCCCCEEHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCHHHEEEEEECC
YSRERRFLLEVKVCRDGQMHRRSTAINEVVLHPGKVAHMIEFEVYINDTFAFSQRSDGLI
CCCCEEEEEEEEECCCCCHHHHHHHHHHHEECCCCEEEEEEEEEEEECEEEECCCCCCEE
ISTPTGSTAYSLSAGGPILAPTLDAIALVPMFPHTLSARPLVIDSNSKIHLRFSHFSNEL
EECCCCCCEEEECCCCCEECCCHHHHEEECCCCCCCCCCEEEEECCCEEEEEEECCCCEE
EISCDSQIALPIQQGEEVIVQRSPFYLSLIHPKDYSYFNTLSSKLGWSKKLF
EEEECCEEEEECCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA