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The map label for this gene is rlmH

Identifier: 238920815

GI number: 238920815

Start: 2829045

End: 2829515

Strand: Direct

Name: rlmH

Synonym: NT01EI_2941

Alternate gene names: 238920815

Gene position: 2829045-2829515 (Clockwise)

Preceding gene: 238920814

Following gene: 238920816

Centisome position: 74.21

GC content: 60.08

Gene sequence:

>471_bases
GTGAAACTGCAGCTGGTGGCGGTCGGCACCAAAATGCCCGACTGGGTACAAACCGGTTTTCTCGACTATATTAAGCGTTT
TCCCAAGGATATGCCGTTTGAACTGACGGAGGTTCCGGCAGGAAAGCGCGGTAAGAATGCCGATATTCGCCGCATTCTGG
AAAGAGAGGGCGAGCTGATGCTGGCCGCAGTCGGTAAAGGCAACCGCATTGTCACCCTGGATATTCCCGGTAAACCCTGG
GATACCCCGGCGCTGGCCGGTCAGCTGGAGCGCTGGAAGCAGGATGGGCGCGACGTCAGCCTGCTGATTGGCGGTCCCGA
AGGGCTGGCCCCCACCTGCAAGGGCGCTGCCGAACAGAGCTGGTCTCTGTCGCCTCTGACCTTGCCGCACCCGCTGGTGC
GCGTGTTGGTGGCCGAAAGCCTCTACCGGGCCTGGAGCATTACGGCCAATCACCCTTATCATCGTGAGTAA

Upstream 100 bases:

>100_bases
GCCGAATGGGTGGTGGTCGATCTCGGCGATGTTATCGTACACGTGATGCAGGAAGAGAGCCGCCGTCTGTACGAACTGGA
AAAGTTGTGGAGCTGATGCG

Downstream 100 bases:

>100_bases
TCGCCTGCCGGCCATGCCGGCAATAATGATGTTAATCCCGGCAGTACGCCTGAGCATGACGGAACGGAATGAAACCAGAT
CGTAACCCCTTTCGTGACTA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKLQLVAVGTKMPDWVQTGFLDYIKRFPKDMPFELTEVPAGKRGKNADIRRILEREGELMLAAVGKGNRIVTLDIPGKPW
DTPALAGQLERWKQDGRDVSLLIGGPEGLAPTCKGAAEQSWSLSPLTLPHPLVRVLVAESLYRAWSITANHPYHRE

Sequences:

>Translated_156_residues
MKLQLVAVGTKMPDWVQTGFLDYIKRFPKDMPFELTEVPAGKRGKNADIRRILEREGELMLAAVGKGNRIVTLDIPGKPW
DTPALAGQLERWKQDGRDVSLLIGGPEGLAPTCKGAAEQSWSLSPLTLPHPLVRVLVAESLYRAWSITANHPYHRE
>Mature_156_residues
MKLQLVAVGTKMPDWVQTGFLDYIKRFPKDMPFELTEVPAGKRGKNADIRRILEREGELMLAAVGKGNRIVTLDIPGKPW
DTPALAGQLERWKQDGRDVSLLIGGPEGLAPTCKGAAEQSWSLSPLTLPHPLVRVLVAESLYRAWSITANHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=86.5384615384615, Blast_Score=281, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_EDWI9 (C5BGD2)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934330.1
- ProteinModelPortal:   C5BGD2
- GeneID:   7960219
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_2941
- OMA:   TANHPYH
- ProtClustDB:   CLSK2813511
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17364; Mature: 17364

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQLVAVGTKMPDWVQTGFLDYIKRFPKDMPFELTEVPAGKRGKNADIRRILEREGELM
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCEE
LAAVGKGNRIVTLDIPGKPWDTPALAGQLERWKQDGRDVSLLIGGPEGLAPTCKGAAEQS
EEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCC
WSLSPLTLPHPLVRVLVAESLYRAWSITANHPYHRE
CCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCC
>Mature Secondary Structure
MKLQLVAVGTKMPDWVQTGFLDYIKRFPKDMPFELTEVPAGKRGKNADIRRILEREGELM
CEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCEE
LAAVGKGNRIVTLDIPGKPWDTPALAGQLERWKQDGRDVSLLIGGPEGLAPTCKGAAEQS
EEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCC
WSLSPLTLPHPLVRVLVAESLYRAWSITANHPYHRE
CCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA