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The map label for this gene is lysS [H]

Identifier: 238917592

GI number: 238917592

Start: 1730612

End: 1732216

Strand: Reverse

Name: lysS [H]

Synonym: EUBELI_01671

Alternate gene names: 238917592

Gene position: 1732216-1730612 (Counterclockwise)

Preceding gene: 238917593

Following gene: 238917591

Centisome position: 80.79

GC content: 42.31

Gene sequence:

>1605_bases
ATGGGAGATAATAAATCTGCACAGCAGCCTGACGTTAACGAGCAGATTAAGGTTCGTATGGAAAAGTTAAAGAACCTTCA
GGACGCAGGTAAGGATCCATTCCAGATAACAAAGTATGATGTAACACATCACACAGATGAAATCAGAGCCATCTATGAAG
CACATGAGAAGGAACTTCTTGGTGACAGACCAGCAGTTAATACTGAAGGTTTAGATGAGCAGCAGGCAAGAGAAGCAGTT
AATGCCGATTACAATGAGAGAAGAGCCATTATGGACGCTTCTCCAATTAATGTAAGCTTTGCCGGACGTATGATGTTCAA
GAGAGTAATGGGAAAGGCATCTTTCTGTAACATTGCTGATCTTAAGGGCAGAATGCAGGCATATATTTCAAGAGATGCAA
TTGGCGAAGAAGCATATGCAGACTTCAAGAAGTCTGATATCGGTGATATCTTCGGAATTAAGGGATTTATCTTCAGAACT
AAGACAGGAGAGATTTCAGTTCACGCTGAGGAGATTACTCTTCTTTCTAAGAGTTTACAGGTACTTCCAGAGAAGTTCCA
CGGAATCACAGATACTGATATGAGATACCGTCAGAGATATGTTGATTTAATAATGAATCCTGAGGTTAAGGAAACATTTG
TAAAGAGATCTAAGATTATCAAGGAAATCAGAAAGTTCCTTGATGGCAGAGACTTCATGGAAGTTGAGACACCAACACTT
GTATCTAATGCTGGTGGTGCTGCCGCAAGACCATTCGAGACTCATTACAACGCACTTGACGAGGACGTTAAGCTTCGTAT
TTCATTAGAGCTTTATCTTAAGAGACTTATCGTTGGTGGTCTTGAGAGAGTTTACGAAATCGGACGAGTATACCGTAACG
AGGGTGTTGATACAAGACACAACCCTGAGTTCACACTTATGGAACTTTACCAGGCATACACAGATTACGAAGGAATGATG
GAACTTACTGAGAGCATGTTCAGACACCTTGCCGAGACTGTATGCGGTACAACTAAGATTACTTACAACGGCACTGAGAT
TGACCTTGGAAAGCCATTCAGAAGACTTACTATGAATGACGCAATTAAGGAATACGCCGGTGTTGATTTTGATACAATTA
AGACTGACGAAGAAGCTAAGGCTCTTGCTAAGGAGAGAGGAATTGAGTTCGAGGAGCGTCACACTAAGGGCGACATCATC
AATCTCTTCTTTGAGGAGTACTGCGAGGAGAATTTAATCCAGCCTACATTCATTATGGATCATCCTCTTGCAATATCTCC
ACTTACTAAGAAGAAGCCATCTGATCCAGAGAAGGTTGAACGTTTCGAGCTTTTCATTAACACATGGGAAATGTGTAATG
CATACTCAGAGCTTAACGACCCAATCGACCAGAGAGAGCGTTTCGCACAGCAGGATAAGAACGCAGAGAACGGCGACGAG
GAAGCACAGCACACAGATGAGGACTTCCTTAACGCACTTGCAGTAGGTATGCCACCAACAGGCGGTATCGGTTACGGTAT
TGACAGACTTGTTATGCTGCTTACAGATTCACCTGCAATTAGAGATGTTCTGCTCTTCCCGACAATGAAGTCTATTGATA
AGTAA

Upstream 100 bases:

>100_bases
CTGTTAAGGTAGAAGCACCTGTTGGAGAGATTGAATATACAGTTGTATCTGTAAAGAACGACTAATTTTCAGTAACATAT
AGAAAAGGAGATACTCATTC

Downstream 100 bases:

>100_bases
GAATGCAGGAAAATCAAGGGTTTCAGCACTTGGATGTACTAAAATATATAGTTGGTCAAGGGGTTTTGACCAAGATTTGA
CCAATTTTTGGCATAGTCAA

Product: lysyl-tRNA synthetase, class II

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MGDNKSAQQPDVNEQIKVRMEKLKNLQDAGKDPFQITKYDVTHHTDEIRAIYEAHEKELLGDRPAVNTEGLDEQQAREAV
NADYNERRAIMDASPINVSFAGRMMFKRVMGKASFCNIADLKGRMQAYISRDAIGEEAYADFKKSDIGDIFGIKGFIFRT
KTGEISVHAEEITLLSKSLQVLPEKFHGITDTDMRYRQRYVDLIMNPEVKETFVKRSKIIKEIRKFLDGRDFMEVETPTL
VSNAGGAAARPFETHYNALDEDVKLRISLELYLKRLIVGGLERVYEIGRVYRNEGVDTRHNPEFTLMELYQAYTDYEGMM
ELTESMFRHLAETVCGTTKITYNGTEIDLGKPFRRLTMNDAIKEYAGVDFDTIKTDEEAKALAKERGIEFEERHTKGDII
NLFFEEYCEENLIQPTFIMDHPLAISPLTKKKPSDPEKVERFELFINTWEMCNAYSELNDPIDQRERFAQQDKNAENGDE
EAQHTDEDFLNALAVGMPPTGGIGYGIDRLVMLLTDSPAIRDVLLFPTMKSIDK

Sequences:

>Translated_534_residues
MGDNKSAQQPDVNEQIKVRMEKLKNLQDAGKDPFQITKYDVTHHTDEIRAIYEAHEKELLGDRPAVNTEGLDEQQAREAV
NADYNERRAIMDASPINVSFAGRMMFKRVMGKASFCNIADLKGRMQAYISRDAIGEEAYADFKKSDIGDIFGIKGFIFRT
KTGEISVHAEEITLLSKSLQVLPEKFHGITDTDMRYRQRYVDLIMNPEVKETFVKRSKIIKEIRKFLDGRDFMEVETPTL
VSNAGGAAARPFETHYNALDEDVKLRISLELYLKRLIVGGLERVYEIGRVYRNEGVDTRHNPEFTLMELYQAYTDYEGMM
ELTESMFRHLAETVCGTTKITYNGTEIDLGKPFRRLTMNDAIKEYAGVDFDTIKTDEEAKALAKERGIEFEERHTKGDII
NLFFEEYCEENLIQPTFIMDHPLAISPLTKKKPSDPEKVERFELFINTWEMCNAYSELNDPIDQRERFAQQDKNAENGDE
EAQHTDEDFLNALAVGMPPTGGIGYGIDRLVMLLTDSPAIRDVLLFPTMKSIDK
>Mature_533_residues
GDNKSAQQPDVNEQIKVRMEKLKNLQDAGKDPFQITKYDVTHHTDEIRAIYEAHEKELLGDRPAVNTEGLDEQQAREAVN
ADYNERRAIMDASPINVSFAGRMMFKRVMGKASFCNIADLKGRMQAYISRDAIGEEAYADFKKSDIGDIFGIKGFIFRTK
TGEISVHAEEITLLSKSLQVLPEKFHGITDTDMRYRQRYVDLIMNPEVKETFVKRSKIIKEIRKFLDGRDFMEVETPTLV
SNAGGAAARPFETHYNALDEDVKLRISLELYLKRLIVGGLERVYEIGRVYRNEGVDTRHNPEFTLMELYQAYTDYEGMME
LTESMFRHLAETVCGTTKITYNGTEIDLGKPFRRLTMNDAIKEYAGVDFDTIKTDEEAKALAKERGIEFEERHTKGDIIN
LFFEEYCEENLIQPTFIMDHPLAISPLTKKKPSDPEKVERFELFINTWEMCNAYSELNDPIDQRERFAQQDKNAENGDEE
AQHTDEDFLNALAVGMPPTGGIGYGIDRLVMLLTDSPAIRDVLLFPTMKSIDK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=455, Percent_Identity=43.7362637362637, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI194272210, Length=455, Percent_Identity=43.7362637362637, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI45439306, Length=466, Percent_Identity=23.3905579399142, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI40789249, Length=243, Percent_Identity=23.4567901234568, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1789256, Length=524, Percent_Identity=48.4732824427481, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI1790571, Length=442, Percent_Identity=53.6199095022624, Blast_Score=487, Evalue=1e-138,
Organism=Escherichia coli, GI87082379, Length=332, Percent_Identity=27.710843373494, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788173, Length=280, Percent_Identity=26.4285714285714, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=455, Percent_Identity=45.4945054945055, Blast_Score=374, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17535927, Length=462, Percent_Identity=44.8051948051948, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71994340, Length=455, Percent_Identity=45.4945054945055, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32566633, Length=281, Percent_Identity=27.7580071174377, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17551876, Length=357, Percent_Identity=24.6498599439776, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=459, Percent_Identity=44.4444444444444, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324256, Length=556, Percent_Identity=31.294964028777, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6323011, Length=354, Percent_Identity=25.9887005649718, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6325153, Length=170, Percent_Identity=31.7647058823529, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321807, Length=443, Percent_Identity=22.7990970654628, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640849, Length=456, Percent_Identity=43.859649122807, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640851, Length=456, Percent_Identity=43.859649122807, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24584738, Length=325, Percent_Identity=26.4615384615385, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17136276, Length=462, Percent_Identity=21.4285714285714, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19922416, Length=417, Percent_Identity=22.3021582733813, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 61187; Mature: 61056

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDNKSAQQPDVNEQIKVRMEKLKNLQDAGKDPFQITKYDVTHHTDEIRAIYEAHEKELL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHC
GDRPAVNTEGLDEQQAREAVNADYNERRAIMDASPINVSFAGRMMFKRVMGKASFCNIAD
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCHHH
LKGRMQAYISRDAIGEEAYADFKKSDIGDIFGIKGFIFRTKTGEISVHAEEITLLSKSLQ
HHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCEEEEEECCCCEEEEHHHHHHHHHHHH
VLPEKFHGITDTDMRYRQRYVDLIMNPEVKETFVKRSKIIKEIRKFLDGRDFMEVETPTL
HHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
VSNAGGAAARPFETHYNALDEDVKLRISLELYLKRLIVGGLERVYEIGRVYRNEGVDTRH
CCCCCCCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NPEFTLMELYQAYTDYEGMMELTESMFRHLAETVCGTTKITYNGTEIDLGKPFRRLTMND
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCCCHHHHHHHH
AIKEYAGVDFDTIKTDEEAKALAKERGIEFEERHTKGDIINLFFEEYCEENLIQPTFIMD
HHHHHHCCCCHHCCCCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEC
HPLAISPLTKKKPSDPEKVERFELFINTWEMCNAYSELNDPIDQRERFAQQDKNAENGDE
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCH
EAQHTDEDFLNALAVGMPPTGGIGYGIDRLVMLLTDSPAIRDVLLFPTMKSIDK
HHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
GDNKSAQQPDVNEQIKVRMEKLKNLQDAGKDPFQITKYDVTHHTDEIRAIYEAHEKELL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHC
GDRPAVNTEGLDEQQAREAVNADYNERRAIMDASPINVSFAGRMMFKRVMGKASFCNIAD
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCHHH
LKGRMQAYISRDAIGEEAYADFKKSDIGDIFGIKGFIFRTKTGEISVHAEEITLLSKSLQ
HHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCEEEEEECCCCEEEEHHHHHHHHHHHH
VLPEKFHGITDTDMRYRQRYVDLIMNPEVKETFVKRSKIIKEIRKFLDGRDFMEVETPTL
HHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
VSNAGGAAARPFETHYNALDEDVKLRISLELYLKRLIVGGLERVYEIGRVYRNEGVDTRH
CCCCCCCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NPEFTLMELYQAYTDYEGMMELTESMFRHLAETVCGTTKITYNGTEIDLGKPFRRLTMND
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCCCHHHHHHHH
AIKEYAGVDFDTIKTDEEAKALAKERGIEFEERHTKGDIINLFFEEYCEENLIQPTFIMD
HHHHHHCCCCHHCCCCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEC
HPLAISPLTKKKPSDPEKVERFELFINTWEMCNAYSELNDPIDQRERFAQQDKNAENGDE
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCH
EAQHTDEDFLNALAVGMPPTGGIGYGIDRLVMLLTDSPAIRDVLLFPTMKSIDK
HHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA