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The map label for this gene is garL [H]

Identifier: 238896637

GI number: 238896637

Start: 4615490

End: 4616260

Strand: Reverse

Name: garL [H]

Synonym: KP1_4841

Alternate gene names: 238896637

Gene position: 4616260-4615490 (Counterclockwise)

Preceding gene: 238896638

Following gene: 238896636

Centisome position: 87.95

GC content: 60.31

Gene sequence:

>771_bases
ATGGATAACGCAATTTTTCCGAACAAATTTAAGGCCGCGCTGGCGGCGCATCAGGTGCAGATTGGCTGCTGGTGCGCTTT
GGCTAACCCGATTAGCACCGAGGTGCTGGGTCTGGCGGGGTTCGACTGGCTGGTGCTGGACGCGGAACACGCGCCAAACG
ACGTCACCACGCTCATTCCGCAGCTGATGGCCCTGAAAGGTAGCTCCAGCGCGCAGGTAGTCCGTGTGCCGACCAATGAA
CCCATCATCATCAAGCGCATGCTGGACATCGGCTTCTATAACTTCCTGGTGCCGTTTGTCGAGACGGCTGAACAGGCGGC
GCAGGCGGTGGCTTCGACCCGTTATCCGCCGGAAGGGATCCGCGGGGTCTCCGTTTCCCACCGCGGCAATATGTTTGGCA
CCGTGCCGGACTACTTCGCCCAGTCCAACAAGAACATTTCCATTCTGGTGCAGATTGAGAGCCAGACCGGAGTGGACAAC
GTCGAGGCGATTGCCGCGACCGAGGGCGTGGACGGTGTGTTTGTCGGCCCGAGCGACCTGGCCGCCGCCTTAGGTCATCT
GGGCAATGCCGCCCATCCTGAGGTGCAGCGCGCTATCCAACATATCTTCGCCAGCGCGAAGAAGCACGGTAAGCCAAGCG
GCATTCTGGCGCCAGTGGAAGCCGATGCGCGCCGCTATCTGGAATGGGGCGCGACCTTCGTCGCCGTTGGCAGCGATCTG
GGCGTCTTCCGTTCTGCTACCCAGAAACTTGCCGACGCGTTTAAAAAATAA

Upstream 100 bases:

>100_bases
GCTCCGCGCTGATGATGATGGTCTGCTACCTGTTTGTGGTTGGCGATATCAAACGTATGGAATTGCAGAAGTAAGCAACG
ATTAACGCAAGGTAAAAATC

Downstream 100 bases:

>100_bases
TCACCAGGATGAAGAGAGAGAACATTATGACAATCAAAGTAGGTTTTATCGGTCTTGGCATTATGGGCAAACCGATGAGC
AAAAATTTGCTGAAAGCGGG

Product: alpha-dehydro-beta-deoxy-D-glucarate aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MDNAIFPNKFKAALAAHQVQIGCWCALANPISTEVLGLAGFDWLVLDAEHAPNDVTTLIPQLMALKGSSSAQVVRVPTNE
PIIIKRMLDIGFYNFLVPFVETAEQAAQAVASTRYPPEGIRGVSVSHRGNMFGTVPDYFAQSNKNISILVQIESQTGVDN
VEAIAATEGVDGVFVGPSDLAAALGHLGNAAHPEVQRAIQHIFASAKKHGKPSGILAPVEADARRYLEWGATFVAVGSDL
GVFRSATQKLADAFKK

Sequences:

>Translated_256_residues
MDNAIFPNKFKAALAAHQVQIGCWCALANPISTEVLGLAGFDWLVLDAEHAPNDVTTLIPQLMALKGSSSAQVVRVPTNE
PIIIKRMLDIGFYNFLVPFVETAEQAAQAVASTRYPPEGIRGVSVSHRGNMFGTVPDYFAQSNKNISILVQIESQTGVDN
VEAIAATEGVDGVFVGPSDLAAALGHLGNAAHPEVQRAIQHIFASAKKHGKPSGILAPVEADARRYLEWGATFVAVGSDL
GVFRSATQKLADAFKK
>Mature_256_residues
MDNAIFPNKFKAALAAHQVQIGCWCALANPISTEVLGLAGFDWLVLDAEHAPNDVTTLIPQLMALKGSSSAQVVRVPTNE
PIIIKRMLDIGFYNFLVPFVETAEQAAQAVASTRYPPEGIRGVSVSHRGNMFGTVPDYFAQSNKNISILVQIESQTGVDN
VEAIAATEGVDGVFVGPSDLAAALGHLGNAAHPEVQRAIQHIFASAKKHGKPSGILAPVEADARRYLEWGATFVAVGSDL
GVFRSATQKLADAFKK

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=256, Percent_Identity=87.109375, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1788578, Length=254, Percent_Identity=43.7007874015748, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 27261; Mature: 27261

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNAIFPNKFKAALAAHQVQIGCWCALANPISTEVLGLAGFDWLVLDAEHAPNDVTTLIP
CCCCCCCHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
QLMALKGSSSAQVVRVPTNEPIIIKRMLDIGFYNFLVPFVETAEQAAQAVASTRYPPEGI
HHHHHCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RGVSVSHRGNMFGTVPDYFAQSNKNISILVQIESQTGVDNVEAIAATEGVDGVFVGPSDL
CCCEECCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCCCCEEEECHHHH
AAALGHLGNAAHPEVQRAIQHIFASAKKHGKPSGILAPVEADARRYLEWGATFVAVGSDL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHCCEEEEECCCH
GVFRSATQKLADAFKK
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDNAIFPNKFKAALAAHQVQIGCWCALANPISTEVLGLAGFDWLVLDAEHAPNDVTTLIP
CCCCCCCHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
QLMALKGSSSAQVVRVPTNEPIIIKRMLDIGFYNFLVPFVETAEQAAQAVASTRYPPEGI
HHHHHCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
RGVSVSHRGNMFGTVPDYFAQSNKNISILVQIESQTGVDNVEAIAATEGVDGVFVGPSDL
CCCEECCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCCCCEEEECHHHH
AAALGHLGNAAHPEVQRAIQHIFASAKKHGKPSGILAPVEADARRYLEWGATFVAVGSDL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHCCEEEEECCCH
GVFRSATQKLADAFKK
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA