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The map label for this gene is nadB [H]

Identifier: 238896025

GI number: 238896025

Start: 3986343

End: 3987962

Strand: Direct

Name: nadB [H]

Synonym: KP1_4156

Alternate gene names: 238896025

Gene position: 3986343-3987962 (Clockwise)

Preceding gene: 238896013

Following gene: 238896027

Centisome position: 75.95

GC content: 59.81

Gene sequence:

>1620_bases
ATGAATACCACTCCTGACTTCTCTTGTGATGTGTTGATTATCGGCAGCGGCGCTGCCGGTCTTTCCCTTGCCCTGCGCCT
GGCTGAGCACAGTTCGGTGACAGTGCTGAGTAAAGGGCCGATCAGCGAAGGTTCGACGTTCTACGCCCAGGGTGGGATTG
CCGCCGTCTTCGATGAGACGGACAGTATTGAATCCCATGTCGAAGATACCCTCATCGCCGGCGCGGGGCTCTGCGATCTT
CATGCGGTCACCTTCGTCGCCAGCAACGCCCGATCATGCGTCCAGTGGCTTATCGATCAGGGAGTGCTGTTTGACACCCA
GGTCCAGGCCAATGGCGAAGAGAGCTATCATCTCACGCGCGAAGGGGGGCACAGCCATCGGCGCATTCTTCACGCCGCCG
ACGCCACCGGCAAAGCGGTAGAGACGACGCTGGTCGATAAAGCGCTTGCCCATCCTAATATCCGCATCCTTGAGCGCAGC
AATGCCGTCGATTTGATCGTATCGGATAAGATTGGCCTGCCGGGCACCCGCCGGGTGGTCGGGGCATGGATCTGGAATCG
TAATAAAGAGCGGGTGGAAACCTGCAGCGCGAAAGCCGTAGTACTCGCTACCGGCGGGGCCGCGAAGGTTTACCAATATA
CCACTAACCCCGACGTCTCTTCCGGAGACGGGATTGCGATGGCCTGGCGGGCGGGATGTCGGGTGGCGAATCTGGAGTTT
AACCAGTTCCACCCCACCGCGCTATATCACCCCCAGGCGCGTAATTTCCTGCTTACTGAAGCGCTGCGCGGCGAAGGCGC
CCACCTTAAACGCCCGGATGGCACCCGCTTTATGCCGGACTTTGACGAGCGCGGCGAGCTGGCCCCGCGCGATATCGTCG
CCCGCGCCATCGACCATGAAATGAAGCGCCTGGGCGTAGACTGTATGTTCCTTGATATCAGCCATAAACCCGAGGCGTTT
GTTCGCCAGCATTTCCCGATGATTTATGAAAAGCTGCTCGGCCTGGGAATTGATTTAACCAAAGAGCCGGTGCCGGTCGT
CCCCGCCGCGCATTACACCTGCGGCGGCGTGATGGTGGATGATAATGGCCGCACCGACGTCGATGGTTTATACGCCATCG
GCGAAGTTAGCTACACCGGGCTGCACGGCGCGAATCGTATGGCGTCGAACTCGTTGCTGGAGTGCCTGGTCTATGGCTGG
TCCGCCGCGGAGGATATTGCCCGGCGCCTGCCGCTCGCCCAGAAGGTCGCCACGCTGCCAGCATGGGATGAAAGTCAGGT
GGAGATCCCGGATGAACTGGTGGTTATCCAGCACAACTGGCACGAACTGCGCCTGCTGATGTGGGATTACGTGGGGATTG
TGCGCACCACCCGCAGGCTGGAGCGCGCCCTGCGGCGCATTACCATGCTCCAGCAGGAGCTGGATGAGTATTACGCCCGC
TTCCGCGTCTCGAATAATTTGCTGGAACTGCGCAATCTGGTGCAGGTGGCGGAGCTTATCGTACGCTGCGCGATGTTGCG
TAAGGAGAGCCGCGGTCTGCATTATACCCTCGACTATCCGCAGCCGCTGCCTGACAGCGGCCCGTCGATACTCTCGCCCC
TGGCTCACATAAAGAGATAA

Upstream 100 bases:

>100_bases
TTTTGTTTAGCCGTCACAACAGCAGACAAAAAAATTGTACGATTCCTCACGGACCGGTGCTATTGTGAGCTAAATGTGTT
TAGTAAATTAAACAAAGACA

Downstream 100 bases:

>100_bases
AAGTCCTCGGTCAGAGCGGTAAAGCCTTCCGAATATTGCTGCTCTGGCCCGCGGATCGCCAGCCTGTCGCTGAAACACTC
CCCTGCCGTCGGGGAGAAAG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MNTTPDFSCDVLIIGSGAAGLSLALRLAEHSSVTVLSKGPISEGSTFYAQGGIAAVFDETDSIESHVEDTLIAGAGLCDL
HAVTFVASNARSCVQWLIDQGVLFDTQVQANGEESYHLTREGGHSHRRILHAADATGKAVETTLVDKALAHPNIRILERS
NAVDLIVSDKIGLPGTRRVVGAWIWNRNKERVETCSAKAVVLATGGAAKVYQYTTNPDVSSGDGIAMAWRAGCRVANLEF
NQFHPTALYHPQARNFLLTEALRGEGAHLKRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGVDCMFLDISHKPEAF
VRQHFPMIYEKLLGLGIDLTKEPVPVVPAAHYTCGGVMVDDNGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAEDIARRLPLAQKVATLPAWDESQVEIPDELVVIQHNWHELRLLMWDYVGIVRTTRRLERALRRITMLQQELDEYYAR
FRVSNNLLELRNLVQVAELIVRCAMLRKESRGLHYTLDYPQPLPDSGPSILSPLAHIKR

Sequences:

>Translated_539_residues
MNTTPDFSCDVLIIGSGAAGLSLALRLAEHSSVTVLSKGPISEGSTFYAQGGIAAVFDETDSIESHVEDTLIAGAGLCDL
HAVTFVASNARSCVQWLIDQGVLFDTQVQANGEESYHLTREGGHSHRRILHAADATGKAVETTLVDKALAHPNIRILERS
NAVDLIVSDKIGLPGTRRVVGAWIWNRNKERVETCSAKAVVLATGGAAKVYQYTTNPDVSSGDGIAMAWRAGCRVANLEF
NQFHPTALYHPQARNFLLTEALRGEGAHLKRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGVDCMFLDISHKPEAF
VRQHFPMIYEKLLGLGIDLTKEPVPVVPAAHYTCGGVMVDDNGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAEDIARRLPLAQKVATLPAWDESQVEIPDELVVIQHNWHELRLLMWDYVGIVRTTRRLERALRRITMLQQELDEYYAR
FRVSNNLLELRNLVQVAELIVRCAMLRKESRGLHYTLDYPQPLPDSGPSILSPLAHIKR
>Mature_539_residues
MNTTPDFSCDVLIIGSGAAGLSLALRLAEHSSVTVLSKGPISEGSTFYAQGGIAAVFDETDSIESHVEDTLIAGAGLCDL
HAVTFVASNARSCVQWLIDQGVLFDTQVQANGEESYHLTREGGHSHRRILHAADATGKAVETTLVDKALAHPNIRILERS
NAVDLIVSDKIGLPGTRRVVGAWIWNRNKERVETCSAKAVVLATGGAAKVYQYTTNPDVSSGDGIAMAWRAGCRVANLEF
NQFHPTALYHPQARNFLLTEALRGEGAHLKRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGVDCMFLDISHKPEAF
VRQHFPMIYEKLLGLGIDLTKEPVPVVPAAHYTCGGVMVDDNGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAEDIARRLPLAQKVATLPAWDESQVEIPDELVVIQHNWHELRLLMWDYVGIVRTTRRLERALRRITMLQQELDEYYAR
FRVSNNLLELRNLVQVAELIVRCAMLRKESRGLHYTLDYPQPLPDSGPSILSPLAHIKR

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=512, Percent_Identity=32.8125, Blast_Score=189, Evalue=6e-48,
Organism=Escherichia coli, GI1788928, Length=536, Percent_Identity=88.9925373134328, Blast_Score=1001, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=525, Percent_Identity=33.1428571428571, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI1786942, Length=561, Percent_Identity=31.0160427807487, Blast_Score=171, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17550100, Length=536, Percent_Identity=31.1567164179104, Blast_Score=188, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17505833, Length=528, Percent_Identity=30.4924242424242, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71986328, Length=455, Percent_Identity=28.3516483516484, Blast_Score=108, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6322701, Length=557, Percent_Identity=32.3159784560144, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6322416, Length=560, Percent_Identity=32.1428571428571, Blast_Score=195, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6320788, Length=490, Percent_Identity=26.9387755102041, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6322511, Length=488, Percent_Identity=25.6147540983607, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24663005, Length=553, Percent_Identity=30.9222423146474, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI17137288, Length=540, Percent_Identity=32.037037037037, Blast_Score=185, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24655642, Length=540, Percent_Identity=32.037037037037, Blast_Score=185, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24655647, Length=540, Percent_Identity=32.037037037037, Blast_Score=185, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59599; Mature: 59599

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTPDFSCDVLIIGSGAAGLSLALRLAEHSSVTVLSKGPISEGSTFYAQGGIAAVFDET
CCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCEEEEECCC
DSIESHVEDTLIAGAGLCDLHAVTFVASNARSCVQWLIDQGVLFDTQVQANGEESYHLTR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEC
EGGHSHRRILHAADATGKAVETTLVDKALAHPNIRILERSNAVDLIVSDKIGLPGTRRVV
CCCCCHHHEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCHHHE
GAWIWNRNKERVETCSAKAVVLATGGAAKVYQYTTNPDVSSGDGIAMAWRAGCRVANLEF
EHHHHCCCHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCEEEEEEC
NQFHPTALYHPQARNFLLTEALRGEGAHLKRPDGTRFMPDFDERGELAPRDIVARAIDHE
CCCCCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MKRLGVDCMFLDISHKPEAFVRQHFPMIYEKLLGLGIDLTKEPVPVVPAAHYTCGGVMVD
HHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCEEEC
DNGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAEDIARRLPLAQKVATLP
CCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHCCC
AWDESQVEIPDELVVIQHNWHELRLLMWDYVGIVRTTRRLERALRRITMLQQELDEYYAR
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRVSNNLLELRNLVQVAELIVRCAMLRKESRGLHYTLDYPQPLPDSGPSILSPLAHIKR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNTTPDFSCDVLIIGSGAAGLSLALRLAEHSSVTVLSKGPISEGSTFYAQGGIAAVFDET
CCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCEEEEECCC
DSIESHVEDTLIAGAGLCDLHAVTFVASNARSCVQWLIDQGVLFDTQVQANGEESYHLTR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEC
EGGHSHRRILHAADATGKAVETTLVDKALAHPNIRILERSNAVDLIVSDKIGLPGTRRVV
CCCCCHHHEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCHHHE
GAWIWNRNKERVETCSAKAVVLATGGAAKVYQYTTNPDVSSGDGIAMAWRAGCRVANLEF
EHHHHCCCHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCEEEEEEC
NQFHPTALYHPQARNFLLTEALRGEGAHLKRPDGTRFMPDFDERGELAPRDIVARAIDHE
CCCCCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MKRLGVDCMFLDISHKPEAFVRQHFPMIYEKLLGLGIDLTKEPVPVVPAAHYTCGGVMVD
HHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCEEEC
DNGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAEDIARRLPLAQKVATLP
CCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHCCC
AWDESQVEIPDELVVIQHNWHELRLLMWDYVGIVRTTRRLERALRRITMLQQELDEYYAR
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRVSNNLLELRNLVQVAELIVRCAMLRKESRGLHYTLDYPQPLPDSGPSILSPLAHIKR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]