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The map label for this gene is hisJ [H]

Identifier: 238893545

GI number: 238893545

Start: 1388520

End: 1389296

Strand: Direct

Name: hisJ [H]

Synonym: KP1_1449

Alternate gene names: 238893545

Gene position: 1388520-1389296 (Clockwise)

Preceding gene: 238893544

Following gene: 238893546

Centisome position: 26.46

GC content: 56.37

Gene sequence:

>777_bases
ATGAAAAAATCACTGTTGCTATGGCTGGCATTAATGGCGTCGACGTCGGCGCTGGCCGAAGGGGGAAAGGAGATCCGCTT
CGGCGTCGACCCGACTTTCGCGCCGTTTGAATGGAAAGATCCGCAGGGCAAGCTGGCGGGGTTTGATATCGATTTAGGCA
ACGCCATTTGTCAGCAGCTGCAGGCGAAATGCGTGTGGGTGGAAAGCAATTTCGACGGCATTATCCCGGCGCTGAAGGCG
CGTAAATTCGACGCGATCCTTTCCGGGATGTATATGACCGAGAAGCGCAAAGCGCAGATTGCCTTTAGCGACAAGTTGTA
TAACGGGCCGGTGTTCCTCGTGGCGCGTAAAAATACCCTGCAAGGCAATACGCCGGAACAGCTGAAGGGCAAAACCATCG
GCGTGGAGCAGGGCTCGGCGCAGGAGACGTATGTTAACCAGCACTGGCGGCCGCAGGGGATAAACATTGTGGCCTACCAG
GGGGCCGATAGCGTGGTGCGTGACCTGGAGTCGGGGCGCATCGACGGCGCGGTGCTCTCCGGGATGATGGCCGACTACAG
CTTCCTGCAGCAGCCGCAGGGTAAGGAATTCGCCTTTGTCGGCGGCCATCTGCAGGACGATACGCTGTTTGGCGCCGGGG
CGGCGATAGGTTTGCGTAAAGACGATGAGGCGCTGCGTCAGGAAATTAACGGCGCGATTGCGAAGATTCTTGCCGACGGC
ACCTATAAAAAATTAGCTGGCAAATATTTTAGCTTTGATGTTTATTCCGGGACATAA

Upstream 100 bases:

>100_bases
CCCGGCTACGACTTTAACGATGACATTATCACCACCGGCGCGACGCTGTTCGCCCGTCTGGTGGAAAAACACTGTCGCTA
ATCTCTGGAGAAGAACGCGA

Downstream 100 bases:

>100_bases
TGTCAGAGTGTACCTGCGGGCGTGGAGCGCCCGCCGTAAGATTCCTTTTCTGATTATCCAATCGACCTCCTCTCCTGACC
CCTGTGGTTACCTGAGGGAA

Product: putative extracellular solute-binding protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKKSLLLWLALMASTSALAEGGKEIRFGVDPTFAPFEWKDPQGKLAGFDIDLGNAICQQLQAKCVWVESNFDGIIPALKA
RKFDAILSGMYMTEKRKAQIAFSDKLYNGPVFLVARKNTLQGNTPEQLKGKTIGVEQGSAQETYVNQHWRPQGINIVAYQ
GADSVVRDLESGRIDGAVLSGMMADYSFLQQPQGKEFAFVGGHLQDDTLFGAGAAIGLRKDDEALRQEINGAIAKILADG
TYKKLAGKYFSFDVYSGT

Sequences:

>Translated_258_residues
MKKSLLLWLALMASTSALAEGGKEIRFGVDPTFAPFEWKDPQGKLAGFDIDLGNAICQQLQAKCVWVESNFDGIIPALKA
RKFDAILSGMYMTEKRKAQIAFSDKLYNGPVFLVARKNTLQGNTPEQLKGKTIGVEQGSAQETYVNQHWRPQGINIVAYQ
GADSVVRDLESGRIDGAVLSGMMADYSFLQQPQGKEFAFVGGHLQDDTLFGAGAAIGLRKDDEALRQEINGAIAKILADG
TYKKLAGKYFSFDVYSGT
>Mature_258_residues
MKKSLLLWLALMASTSALAEGGKEIRFGVDPTFAPFEWKDPQGKLAGFDIDLGNAICQQLQAKCVWVESNFDGIIPALKA
RKFDAILSGMYMTEKRKAQIAFSDKLYNGPVFLVARKNTLQGNTPEQLKGKTIGVEQGSAQETYVNQHWRPQGINIVAYQ
GADSVVRDLESGRIDGAVLSGMMADYSFLQQPQGKEFAFVGGHLQDDTLFGAGAAIGLRKDDEALRQEINGAIAKILADG
TYKKLAGKYFSFDVYSGT

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=51.9148936170213, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1788649, Length=258, Percent_Identity=48.062015503876, Blast_Score=254, Evalue=5e-69,
Organism=Escherichia coli, GI1787085, Length=250, Percent_Identity=37.2, Blast_Score=170, Evalue=8e-44,
Organism=Escherichia coli, GI1787088, Length=256, Percent_Identity=32.8125, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787031, Length=245, Percent_Identity=29.7959183673469, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788228, Length=235, Percent_Identity=28.0851063829787, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786876, Length=244, Percent_Identity=27.4590163934426, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28172; Mature: 28172

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLLLWLALMASTSALAEGGKEIRFGVDPTFAPFEWKDPQGKLAGFDIDLGNAICQQL
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHH
QAKCVWVESNFDGIIPALKARKFDAILSGMYMTEKRKAQIAFSDKLYNGPVFLVARKNTL
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEECCCCEEEEEECCCC
QGNTPEQLKGKTIGVEQGSAQETYVNQHWRPQGINIVAYQGADSVVRDLESGRIDGAVLS
CCCCHHHHCCCEEECCCCCCCHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHH
GMMADYSFLQQPQGKEFAFVGGHLQDDTLFGAGAAIGLRKDDEALRQEINGAIAKILADG
HHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCEEECCCCCHHHHHHHHHHHHHHHHCCC
TYKKLAGKYFSFDVYSGT
CHHHHCCCEEEEEEECCC
>Mature Secondary Structure
MKKSLLLWLALMASTSALAEGGKEIRFGVDPTFAPFEWKDPQGKLAGFDIDLGNAICQQL
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHH
QAKCVWVESNFDGIIPALKARKFDAILSGMYMTEKRKAQIAFSDKLYNGPVFLVARKNTL
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEECCCCEEEEEECCCC
QGNTPEQLKGKTIGVEQGSAQETYVNQHWRPQGINIVAYQGADSVVRDLESGRIDGAVLS
CCCCHHHHCCCEEECCCCCCCHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHH
GMMADYSFLQQPQGKEFAFVGGHLQDDTLFGAGAAIGLRKDDEALRQEINGAIAKILADG
HHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCEEECCCCCHHHHHHHHHHHHHHHHCCC
TYKKLAGKYFSFDVYSGT
CHHHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]