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The map label for this gene is yadB [H]

Identifier: 238893123

GI number: 238893123

Start: 954356

End: 955237

Strand: Reverse

Name: yadB [H]

Synonym: KP1_0972

Alternate gene names: 238893123

Gene position: 955237-954356 (Counterclockwise)

Preceding gene: 238893124

Following gene: 238893122

Centisome position: 18.2

GC content: 64.17

Gene sequence:

>882_bases
ATGACTGACTCACGCTATATAGGGCGCTTCGCCCCCTCCCCCTCCGGCGAACTCCACTTCGGCTCTCTGATCGCCGCGCT
CGGCAGCTACCTGCAAGCCCGCGCGAACCAGGGTATCTGGCGTGTTCGTATTGAAGATATCGACCCGCCGCGCGAAGTCC
CCGGCGCCGCCGATACCATCCTGCGCCAGCTTGACCACTACGGTCTGCACTGGGATGGCGACGTGCTGTGGCAGTCGCAA
CGGCACGAAGCCTACCGCGAGGCGCTGACGTGGCTGGGCGAGCAAGGGCTGAGCTATTACTGCACTTGCACCCGCGCGCG
GATCCACGCCGTGGGCGGGATCTACGACGGCCACTGCCGCGACCTCGGGCTGGGGGCGGAAAACGCCGCGCTGCGCCTGC
GCCAAACCCGTCCGGTGCTGCAGTTTAGCGACCGTCTGCGCGGCACGCTCATCGCCAATGAGCCGCTGGCGCGTGAGGAT
TTTATTGTCCACCGTCGCGACGGGCTGTTCGCCTACAACCTGGCGGTGGTGGTCGACGACCACTTTCAGGGGATCACCGA
GATTGTCCGCGGCGCAGACCTGATTGAGCCGACGGTGCGGCAGATTTCGCTGTATCAGCATTTTGGCTGGCAGGCGCCGG
ATTACCTCCATCTGCCGCTGGCTCTCAATGGCGACGGCAATAAACTCTCTAAACAAAACCACGCGCCGGCGCTGCCGGAA
GGCGATCCGCGCCCCGAAATCGTCCGGGCGCTCCGCTTTCTGAATCAGGCGATCCCCGAGGAGTGGCAGGCCCTGAGCAT
CGATGATTTACTCGCCCAGGCCGTCGCCAACTGGCAGCCGGCGAAAATCGAGCATTCTCAAATGGCTCCCGCTGAGCTAT
GA

Upstream 100 bases:

>100_bases
AAACCCTGGCGGAAATTCGCGAAAAACAGATGGCGGGCTAATCCCCTGCTGCCTCACTCTCTGGCGGGAGTCTCTCCCGC
CTGGTTCTGTTGTCCGTACC

Downstream 100 bases:

>100_bases
TTAGCCGCTATTTTTACACTGTACAGTTGATCTGAAGTCCGTCTGACACTACGAGGTGCACCATTTTTACCCGAGTCGCT
AATTTTTGCCGTAAGGTGCT

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTDSRYIGRFAPSPSGELHFGSLIAALGSYLQARANQGIWRVRIEDIDPPREVPGAADTILRQLDHYGLHWDGDVLWQSQ
RHEAYREALTWLGEQGLSYYCTCTRARIHAVGGIYDGHCRDLGLGAENAALRLRQTRPVLQFSDRLRGTLIANEPLARED
FIVHRRDGLFAYNLAVVVDDHFQGITEIVRGADLIEPTVRQISLYQHFGWQAPDYLHLPLALNGDGNKLSKQNHAPALPE
GDPRPEIVRALRFLNQAIPEEWQALSIDDLLAQAVANWQPAKIEHSQMAPAEL

Sequences:

>Translated_293_residues
MTDSRYIGRFAPSPSGELHFGSLIAALGSYLQARANQGIWRVRIEDIDPPREVPGAADTILRQLDHYGLHWDGDVLWQSQ
RHEAYREALTWLGEQGLSYYCTCTRARIHAVGGIYDGHCRDLGLGAENAALRLRQTRPVLQFSDRLRGTLIANEPLARED
FIVHRRDGLFAYNLAVVVDDHFQGITEIVRGADLIEPTVRQISLYQHFGWQAPDYLHLPLALNGDGNKLSKQNHAPALPE
GDPRPEIVRALRFLNQAIPEEWQALSIDDLLAQAVANWQPAKIEHSQMAPAEL
>Mature_292_residues
TDSRYIGRFAPSPSGELHFGSLIAALGSYLQARANQGIWRVRIEDIDPPREVPGAADTILRQLDHYGLHWDGDVLWQSQR
HEAYREALTWLGEQGLSYYCTCTRARIHAVGGIYDGHCRDLGLGAENAALRLRQTRPVLQFSDRLRGTLIANEPLAREDF
IVHRRDGLFAYNLAVVVDDHFQGITEIVRGADLIEPTVRQISLYQHFGWQAPDYLHLPLALNGDGNKLSKQNHAPALPEG
DPRPEIVRALRFLNQAIPEEWQALSIDDLLAQAVANWQPAKIEHSQMAPAEL

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=249, Percent_Identity=36.5461847389558, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI226510925, Length=289, Percent_Identity=75.7785467128028, Blast_Score=459, Evalue=1e-131,
Organism=Escherichia coli, GI1788743, Length=244, Percent_Identity=34.8360655737705, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71993394, Length=297, Percent_Identity=30.976430976431, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6324540, Length=248, Percent_Identity=33.0645161290323, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24665321, Length=249, Percent_Identity=36.5461847389558, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: NA

Molecular weight: Translated: 32947; Mature: 32815

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSRYIGRFAPSPSGELHFGSLIAALGSYLQARANQGIWRVRIEDIDPPREVPGAADTI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHH
LRQLDHYGLHWDGDVLWQSQRHEAYREALTWLGEQGLSYYCTCTRARIHAVGGIYDGHCR
HHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCCCCC
DLGLGAENAALRLRQTRPVLQFSDRLRGTLIANEPLAREDFIVHRRDGLFAYNLAVVVDD
CCCCCCCHHHEEHHHCCCHHHHHHHHCEEEEECCCCCCCCCEEEECCCEEEEEEEEEECH
HFQGITEIVRGADLIEPTVRQISLYQHFGWQAPDYLHLPLALNGDGNKLSKQNHAPALPE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
GDPRPEIVRALRFLNQAIPEEWQALSIDDLLAQAVANWQPAKIEHSQMAPAEL
CCCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDSRYIGRFAPSPSGELHFGSLIAALGSYLQARANQGIWRVRIEDIDPPREVPGAADTI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHH
LRQLDHYGLHWDGDVLWQSQRHEAYREALTWLGEQGLSYYCTCTRARIHAVGGIYDGHCR
HHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCCCCCC
DLGLGAENAALRLRQTRPVLQFSDRLRGTLIANEPLAREDFIVHRRDGLFAYNLAVVVDD
CCCCCCCHHHEEHHHCCCHHHHHHHHCEEEEECCCCCCCCCEEEECCCEEEEEEEEEECH
HFQGITEIVRGADLIEPTVRQISLYQHFGWQAPDYLHLPLALNGDGNKLSKQNHAPALPE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
GDPRPEIVRALRFLNQAIPEEWQALSIDDLLAQAVANWQPAKIEHSQMAPAEL
CCCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA