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The map label for this gene is pcnB [H]

Identifier: 238893122

GI number: 238893122

Start: 952899

End: 954260

Strand: Reverse

Name: pcnB [H]

Synonym: KP1_0971

Alternate gene names: 238893122

Gene position: 954260-952899 (Counterclockwise)

Preceding gene: 238893123

Following gene: 238893121

Centisome position: 18.18

GC content: 61.97

Gene sequence:

>1362_bases
GTGCTAAGCCGTGAGGAGCGCGAGGCAGAAGCCGCCGTCGAACCAACGCCTATGACGGTGATCCCGCGTGAGCAGCATGC
TATTTCCCGCAAAGATATCAGTGAAAACGCCCTCAAGGTCATGTACCGCCTCAACAAGGCGGGCTACGAATCCTGGCTGG
TCGGCGGCGGCGTCCGCGACCTGCTGCTTGGCAAAAAGCCAAAAGATTTTGACGTTACCACCAACGCCACGCCGGATCAG
GTACGCAAGCTGTTCCGCAACTGCCGTCTGGTTGGGCGACGTTTCCGCCTGGCGCATGTGATGTTCGGGCCGGAAATCAT
TGAGGTCGCCACCTTCCGTGGCCATCACGAAGGGCATACCACCGATCGCGTCACCTCCCAGCGGGGCCAGAACGGCATGC
TGCTGCGCGATAACATCTTCGGCTCGATCGAGGAGGATGCCCAGCGTCGCGACTTCACTATCAACAGCCTCTATTACAGC
GTGGCGGACTTTACCGTTCGCGACTACGTCGGCGGCATGAAGGATCTGCAGGACGGCGTGATCCGTCTGATCGGCAACCC
GGAAACCCGCTATCGCGAGGATCCAGTGCGCATGCTGCGCGCGGTGCGCTTTGCCGCCAAGCTCAATATGACCATCAGCC
CGGAAACGGCTGAACCGCTGCCGCGCCTGGCCGCCCTGCTCCACGATGTGCCGCCGGCGCGTCTGTTCGAAGAAGTGCTT
AAGCTGCTGCAGGCCGGGTATGGCTACCAGACCTACATGCTGCTGCGGGAATACAGCCTGTTCCAGCCGCTGTTCCCGAC
CATCACCCGCTACTTTACCGAGCGCGGCGACAGCCCGATGGAGCGGATCATCAGCCAGGTGCTGAAGAATACCGATACCC
GCATTCATAACGATATGCGCGTCAATCCGGCCTTCCTCTTCGCGGTGATGTTCTGGTATCCGCTGCTGGAGACGGCGCAA
AAAATCGCTCAGGAGAGCGGCCTGGCGTACTACGACGCCTTCGCCCTGGCGATGAACGATGTGCTGGACGAAGCCTGCCG
TACGCTGGCGATCCCTAAGCGCATCACCACCCTTATTCGTGATATCTGGCAGCTGCAGCTGCGCATGTCCCGTCGTCAGG
GCAAACGCGCCTGGAAGCTGATGGAGCATCCGAAATTCCGCGCCGCCTATGACCTGCTGGAGCTACGCGCCGGCGCCGAG
AACAACCACGAACTGCAGCGCCTGACCAAATGGTGGGGCGAATTCCAGGTCGCCGCGCCGCCGGCGCAAAAAGATATGCT
CAACGACCTCGGGGACGATCCGGCGCCGCGCCGTCGTCACCGTCGTCCGCGCAAGCGCGCGCCGCGCCAGGGTAGCGCAT
GA

Upstream 100 bases:

>100_bases
TATGATTAGCCGCTATTTTTACACTGTACAGTTGATCTGAAGTCCGTCTGACACTACGAGGTGCACCATTTTTACCCGAG
TCGCTAATTTTTGCCGTAAG

Downstream 100 bases:

>100_bases
CTCTGGTCTATATCGCTCTTGGCAGCAACCTTGCTTCTCCACTGGAGCAGGCGCAGGCGGCTATCCGCGCCCTGGGCGAA
ATTCCGCACAGCCGCGTGGT

Product: poly(A) polymerase I

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MLSREEREAEAAVEPTPMTVIPREQHAISRKDISENALKVMYRLNKAGYESWLVGGGVRDLLLGKKPKDFDVTTNATPDQ
VRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGHTTDRVTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYS
VADFTVRDYVGGMKDLQDGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMTISPETAEPLPRLAALLHDVPPARLFEEVL
KLLQAGYGYQTYMLLREYSLFQPLFPTITRYFTERGDSPMERIISQVLKNTDTRIHNDMRVNPAFLFAVMFWYPLLETAQ
KIAQESGLAYYDAFALAMNDVLDEACRTLAIPKRITTLIRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAYDLLELRAGAE
NNHELQRLTKWWGEFQVAAPPAQKDMLNDLGDDPAPRRRHRRPRKRAPRQGSA

Sequences:

>Translated_453_residues
MLSREEREAEAAVEPTPMTVIPREQHAISRKDISENALKVMYRLNKAGYESWLVGGGVRDLLLGKKPKDFDVTTNATPDQ
VRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGHTTDRVTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYS
VADFTVRDYVGGMKDLQDGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMTISPETAEPLPRLAALLHDVPPARLFEEVL
KLLQAGYGYQTYMLLREYSLFQPLFPTITRYFTERGDSPMERIISQVLKNTDTRIHNDMRVNPAFLFAVMFWYPLLETAQ
KIAQESGLAYYDAFALAMNDVLDEACRTLAIPKRITTLIRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAYDLLELRAGAE
NNHELQRLTKWWGEFQVAAPPAQKDMLNDLGDDPAPRRRHRRPRKRAPRQGSA
>Mature_453_residues
MLSREEREAEAAVEPTPMTVIPREQHAISRKDISENALKVMYRLNKAGYESWLVGGGVRDLLLGKKPKDFDVTTNATPDQ
VRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGHTTDRVTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYS
VADFTVRDYVGGMKDLQDGVIRLIGNPETRYREDPVRMLRAVRFAAKLNMTISPETAEPLPRLAALLHDVPPARLFEEVL
KLLQAGYGYQTYMLLREYSLFQPLFPTITRYFTERGDSPMERIISQVLKNTDTRIHNDMRVNPAFLFAVMFWYPLLETAQ
KIAQESGLAYYDAFALAMNDVLDEACRTLAIPKRITTLIRDIWQLQLRMSRRQGKRAWKLMEHPKFRAAYDLLELRAGAE
NNHELQRLTKWWGEFQVAAPPAQKDMLNDLGDDPAPRRRHRRPRKRAPRQGSA

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=210, Percent_Identity=31.4285714285714, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87081691, Length=433, Percent_Identity=88.9145496535797, Blast_Score=800, Evalue=0.0,
Organism=Escherichia coli, GI1789436, Length=321, Percent_Identity=27.4143302180685, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=286, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321016, Length=188, Percent_Identity=29.2553191489362, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281360112, Length=244, Percent_Identity=29.0983606557377, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357337, Length=244, Percent_Identity=29.0983606557377, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 52401; Mature: 52401

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSREEREAEAAVEPTPMTVIPREQHAISRKDISENALKVMYRLNKAGYESWLVGGGVRD
CCCCHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHH
LLLGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGHT
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
TDRVTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLQDGV
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
IRLIGNPETRYREDPVRMLRAVRFAAKLNMTISPETAEPLPRLAALLHDVPPARLFEEVL
HHHCCCCCHHCCCCHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHH
KLLQAGYGYQTYMLLREYSLFQPLFPTITRYFTERGDSPMERIISQVLKNTDTRIHNDMR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCCC
VNPAFLFAVMFWYPLLETAQKIAQESGLAYYDAFALAMNDVLDEACRTLAIPKRITTLIR
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIWQLQLRMSRRQGKRAWKLMEHPKFRAAYDLLELRAGAENNHELQRLTKWWGEFQVAAP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEECCC
PAQKDMLNDLGDDPAPRRRHRRPRKRAPRQGSA
CHHHHHHHHCCCCCCCHHHHCCCHHCCCCCCCC
>Mature Secondary Structure
MLSREEREAEAAVEPTPMTVIPREQHAISRKDISENALKVMYRLNKAGYESWLVGGGVRD
CCCCHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHH
LLLGKKPKDFDVTTNATPDQVRKLFRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGHT
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCC
TDRVTSQRGQNGMLLRDNIFGSIEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLQDGV
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
IRLIGNPETRYREDPVRMLRAVRFAAKLNMTISPETAEPLPRLAALLHDVPPARLFEEVL
HHHCCCCCHHCCCCHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHH
KLLQAGYGYQTYMLLREYSLFQPLFPTITRYFTERGDSPMERIISQVLKNTDTRIHNDMR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCCC
VNPAFLFAVMFWYPLLETAQKIAQESGLAYYDAFALAMNDVLDEACRTLAIPKRITTLIR
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIWQLQLRMSRRQGKRAWKLMEHPKFRAAYDLLELRAGAENNHELQRLTKWWGEFQVAAP
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEECCC
PAQKDMLNDLGDDPAPRRRHRRPRKRAPRQGSA
CHHHHHHHHCCCCCCCHHHHCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]