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The map label for this gene is rob [H]

Identifier: 238892982

GI number: 238892982

Start: 790363

End: 791232

Strand: Reverse

Name: rob [H]

Synonym: KP1_0813

Alternate gene names: 238892982

Gene position: 791232-790363 (Counterclockwise)

Preceding gene: 238892987

Following gene: 238892980

Centisome position: 15.08

GC content: 57.13

Gene sequence:

>870_bases
ATGGATCAAGCAGGGATTATTCGCGATCTCCTTAGCTGGCTGGAAGGTCACCTGGATCAGCCGCTTTCTCTGGATAATGT
GGCGGCGAAGGCAGGTTATTCCAAGTGGCATTTGCAGCGAATGTTTAAGGATGTCACCGGCCATGCCATCGGTGCCTATA
TTCGCGCGCGCCGCTTGTCGAAATCTGCTGTCGCATTACGCCTGACCGCCCGTCCAATCCTTGATATTGCGCTGCAGTAC
CGTTTCGACTCCCAGCAGACCTTCACCCGAGCGTTTAAGAAACAATTCTCGCTGACGCCGGCGCTCTACCGCCGCTCGCC
GGACTGGAGTTCGTTCGGTATGCGCCCGCCGCTGCGTCTTGGCGAATTTACCCTGCCGAAACATGAGTTTATCACTCTGC
CCCCTACTCAGCTGCTTGGCGTGACCCAGAGCTATACCTGTAAGCTCGAGGAAATCTCCGATTTTCGTAACCAGATGCGG
GTCCAGTTCTGGCGAGACTTCCTCGGCAACTCACCGTCAATCCCCCCGGTGCTCTACGGGCTGCATGAGCCGCGTCCGAG
CCTGGAAAAAGACGATGAGCAGGAAGTATTCTATACCACCGCGCTGACGCCGGAGATGGCTAACGGCCACCTGCAGCATG
CCCATCCAGTGACCCTGGAGGGCGGCGAGTATGTCATGTTTACGTATGAAGGGCTGGGTACCGGTCTGCAGGAATTCATC
CTGACCGTCTACGGCACCTGCATGCCGATGCTGCACCTGACGCGTCGCAAGGGGCTGGATATTGAACGCTTCTACCCTGA
AGATGAAAGCCGGGATCAGGCCACGCCGATTCAGTTGCGCTGCGAGTATCTGATCCCGATCCGCCGTTAA

Upstream 100 bases:

>100_bases
GCATTTAAAAGCAAAAAAACACTGAATGCTAAAACATCAAAAAATGCTATTATCCGCTACTTTAATATCAGGCACCTGTG
TTTAAGGATGGGGACATTTT

Downstream 100 bases:

>100_bases
CGCTGCAGCTCGTCCATTGCGGGGGCGTCAAGATGCGAGACGTCGCCCGCCGTTTCCACCACCCAGCCGGAAGCCAGCCA
CGGGCTTTGCTGATAGTCGA

Product: right origin-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDQAGIIRDLLSWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSAVALRLTARPILDIALQY
RFDSQQTFTRAFKKQFSLTPALYRRSPDWSSFGMRPPLRLGEFTLPKHEFITLPPTQLLGVTQSYTCKLEEISDFRNQMR
VQFWRDFLGNSPSIPPVLYGLHEPRPSLEKDDEQEVFYTTALTPEMANGHLQHAHPVTLEGGEYVMFTYEGLGTGLQEFI
LTVYGTCMPMLHLTRRKGLDIERFYPEDESRDQATPIQLRCEYLIPIRR

Sequences:

>Translated_289_residues
MDQAGIIRDLLSWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSAVALRLTARPILDIALQY
RFDSQQTFTRAFKKQFSLTPALYRRSPDWSSFGMRPPLRLGEFTLPKHEFITLPPTQLLGVTQSYTCKLEEISDFRNQMR
VQFWRDFLGNSPSIPPVLYGLHEPRPSLEKDDEQEVFYTTALTPEMANGHLQHAHPVTLEGGEYVMFTYEGLGTGLQEFI
LTVYGTCMPMLHLTRRKGLDIERFYPEDESRDQATPIQLRCEYLIPIRR
>Mature_289_residues
MDQAGIIRDLLSWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLSKSAVALRLTARPILDIALQY
RFDSQQTFTRAFKKQFSLTPALYRRSPDWSSFGMRPPLRLGEFTLPKHEFITLPPTQLLGVTQSYTCKLEEISDFRNQMR
VQFWRDFLGNSPSIPPVLYGLHEPRPSLEKDDEQEVFYTTALTPEMANGHLQHAHPVTLEGGEYVMFTYEGLGTGLQEFI
LTVYGTCMPMLHLTRRKGLDIERFYPEDESRDQATPIQLRCEYLIPIRR

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=289, Percent_Identity=81.3148788927336, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1790497, Length=104, Percent_Identity=55.7692307692308, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI87081928, Length=101, Percent_Identity=50.4950495049505, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33352; Mature: 33352

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQAGIIRDLLSWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLS
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
KSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFSLTPALYRRSPDWSSFGMRPPLRL
CCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCC
GEFTLPKHEFITLPPTQLLGVTQSYTCKLEEISDFRNQMRVQFWRDFLGNSPSIPPVLYG
CCCCCCCCCEEECCHHHHHCCCCHHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEC
LHEPRPSLEKDDEQEVFYTTALTPEMANGHLQHAHPVTLEGGEYVMFTYEGLGTGLQEFI
CCCCCCCCCCCCCCEEEEEEECCHHHCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHH
LTVYGTCMPMLHLTRRKGLDIERFYPEDESRDQATPIQLRCEYLIPIRR
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure
MDQAGIIRDLLSWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKDVTGHAIGAYIRARRLS
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
KSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFSLTPALYRRSPDWSSFGMRPPLRL
CCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCC
GEFTLPKHEFITLPPTQLLGVTQSYTCKLEEISDFRNQMRVQFWRDFLGNSPSIPPVLYG
CCCCCCCCCEEECCHHHHHCCCCHHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEC
LHEPRPSLEKDDEQEVFYTTALTPEMANGHLQHAHPVTLEGGEYVMFTYEGLGTGLQEFI
CCCCCCCCCCCCCCEEEEEEECCHHHCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHH
LTVYGTCMPMLHLTRRKGLDIERFYPEDESRDQATPIQLRCEYLIPIRR
HHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]