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The map label for this gene is prfC [H]

Identifier: 238892962

GI number: 238892962

Start: 768416

End: 770005

Strand: Direct

Name: prfC [H]

Synonym: KP1_0789

Alternate gene names: 238892962

Gene position: 768416-770005 (Clockwise)

Preceding gene: 238892961

Following gene: 238892963

Centisome position: 14.64

GC content: 56.48

Gene sequence:

>1590_bases
ATGACGTTGTCTCCTTATCTGCAAGAGGTGGCCAAACGCCGCACTTTTGCCATTATTTCTCACCCGGATGCCGGTAAGAC
CACCATCACCGAAAAGGTGCTGCTGTTCGGACAGGCGATTCAGACCGCCGGTACAGTAAAAGGTCGTGGTTCCAGCCAGC
ACGCGAAATCCGACTGGATGGAGATGGAAAAGCAGCGTGGTATCTCGATCACCACCTCGGTAATGCAGTTCCCGTATCAT
GACTGCCTGGTGAACCTGCTGGATACCCCGGGGCACGAAGACTTCTCCGAAGATACCTACCGTACCCTGACGGCGGTGGA
CTGCTGCCTGATGGTGATTGACGCGGCAAAAGGCGTCGAAGATCGGACCCGTAAGCTGATGGAAGTCACCCGTCTGCGCG
ATACGCCGATCCTCACCTTTATGAACAAACTCGACCGTGATATCCGCGATCCGATGGAGCTGCTGGATGAAGTCGAGAAC
GAGCTGAAAATCGGCTGCGCGCCGATTACCTGGCCTATCGGCTGCGGCAAGCTGTTCAAAGGCGTTTATCATCTCTACAA
AGACGAAACCTACCTGTATCAGACCGGTAAAGGCCACACCATCCAGGAAGTGCGTATCGTGAAGGGGCTCAACAACCCGG
ATCTCGACGCGGCGGTGGGGGAAGACCTGGCGCAGCAGCTGCGTGACGAGCTGGAGCTGGTGCAGGGGGCGTCGAATGAG
TTCGACAAAGATCTGTTCCTGGCCGGGGAAATCACCCCGGTCTTCTTCGGAACCGCGTTAGGTAACTTCGGCGTTGACCA
TATGCTGGATGGCCTGGTGGAGTGGGCCCCGGCGCCGATGCCGCGCAACACCGACACGCGTGAAGTCACCGCCACGGAAG
AGAAATTCACCGGCTTCGTGTTTAAAATTCAGGCCAACATGGATCCGAAACACCGCGACCGCGTGGCTTTCATGCGGGTG
GTGTCCGGCAAATATGAAAAAGGTATGAAGCTGCGTCAGGTGCGTATCGGCAAGGACGTAGTGATTTCCGACGCGCTGAC
CTTTATGGCGGGCGATCGCTCGCACGTTGAAGAAGCGTACCCAGGCGATATCATCGGCCTGCATAACCACGGCACCATTC
AGATTGGCGATACCTTTACCCAGGGCGAGATGATGAAGTTCACCGGTATTCCGAACTTCGCGCCGGAACTGTTCCGTCGT
ATTCGCCTGAAGGATCCGCTGAAGCAGAAACAGCTGCTGAAAGGGCTGGTTCAGCTCTCTGAAGAGGGCGCGGTCCAGGT
CTTCCGCCCGATCGCTAATAACGATCTGATCGTTGGCGCCGTCGGCGTGCTGCAGTTTGACGTGGTGGTAGCGCGTCTGA
AAAGCGAATACAACGTGGAAGCGATTTACGAATCGGTCAACGTGGCCACCGCGCGCTGGGTTGAATCTACTGACGTGAAG
AAGTTCGAAGAGTTCAAGCGGAAAAATGAAGTTCAGCTGGCGCTGGATGGCGGCGATAACCTGACCTACATCGCCCCGAC
AATGGTCAACCTGAACCTGACGCAGGAGCGTTACCCTGACGTGGTGTTCCGCAAAACGCGCGAGCACTAA

Upstream 100 bases:

>100_bases
TAAACACTGATTGCCTCCCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTATTCCACATGCGTGGCCCATGGCCGC
GTTTACTTAGAAGATTAATT

Downstream 100 bases:

>100_bases
TCCCTGACGAAGCGCGGCGCAGGCCGCGCTTTTTTCTGGTTACGCCCCCGTTCCCTTCCTTCTGGAAAGTTCTTAAAACA
CCGCTAATTCGTCTGTTTTT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSSQHAKSDWMEMEKQRGISITTSVMQFPYH
DCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQTGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVQGASNE
FDKDLFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRNTDTREVTATEEKFTGFVFKIQANMDPKHRDRVAFMRV
VSGKYEKGMKLRQVRIGKDVVISDALTFMAGDRSHVEEAYPGDIIGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRR
IRLKDPLKQKQLLKGLVQLSEEGAVQVFRPIANNDLIVGAVGVLQFDVVVARLKSEYNVEAIYESVNVATARWVESTDVK
KFEEFKRKNEVQLALDGGDNLTYIAPTMVNLNLTQERYPDVVFRKTREH

Sequences:

>Translated_529_residues
MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSSQHAKSDWMEMEKQRGISITTSVMQFPYH
DCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQTGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVQGASNE
FDKDLFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRNTDTREVTATEEKFTGFVFKIQANMDPKHRDRVAFMRV
VSGKYEKGMKLRQVRIGKDVVISDALTFMAGDRSHVEEAYPGDIIGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRR
IRLKDPLKQKQLLKGLVQLSEEGAVQVFRPIANNDLIVGAVGVLQFDVVVARLKSEYNVEAIYESVNVATARWVESTDVK
KFEEFKRKNEVQLALDGGDNLTYIAPTMVNLNLTQERYPDVVFRKTREH
>Mature_528_residues
TLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSSQHAKSDWMEMEKQRGISITTSVMQFPYHD
CLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQTGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVQGASNEF
DKDLFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRNTDTREVTATEEKFTGFVFKIQANMDPKHRDRVAFMRVV
SGKYEKGMKLRQVRIGKDVVISDALTFMAGDRSHVEEAYPGDIIGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRI
RLKDPLKQKQLLKGLVQLSEEGAVQVFRPIANNDLIVGAVGVLQFDVVVARLKSEYNVEAIYESVNVATARWVESTDVKK
FEEFKRKNEVQLALDGGDNLTYIAPTMVNLNLTQERYPDVVFRKTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=498, Percent_Identity=27.9116465863454, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI19923640, Length=511, Percent_Identity=26.027397260274, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=27.9187817258883, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI25306287, Length=295, Percent_Identity=30.1694915254237, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI157426893, Length=169, Percent_Identity=27.810650887574, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI94966754, Length=164, Percent_Identity=28.6585365853659, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1790835, Length=529, Percent_Identity=95.0850661625709, Blast_Score=1052, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=498, Percent_Identity=25.9036144578313, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI48994988, Length=215, Percent_Identity=30.6976744186047, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=32.0512820512821, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=489, Percent_Identity=28.2208588957055, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17556745, Length=164, Percent_Identity=31.7073170731707, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=30.2631578947368, Blast_Score=76, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=478, Percent_Identity=27.1966527196653, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6322359, Length=429, Percent_Identity=26.5734265734266, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=24.8407643312102, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=157, Percent_Identity=26.1146496815287, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=157, Percent_Identity=26.1146496815287, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24582462, Length=482, Percent_Identity=25.7261410788382, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI221458488, Length=521, Percent_Identity=24.9520153550864, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=32.7044025157233, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59630; Mature: 59499

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSSQHAKSDWM
CCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
EMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVE
HHHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
DRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENELKIGCAPITWPIGCGKLFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHH
GVYHLYKDETYLYQTGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVQGASNE
HHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FDKDLFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRNTDTREVTATEEKFTGFV
CCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHCEEE
FKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVRIGKDVVISDALTFMAGDRSHVEEAY
EEEEECCCCCHHHHHHHHHHHHCHHHCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHCC
PGDIIGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLS
CCCEEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
EEGAVQVFRPIANNDLIVGAVGVLQFDVVVARLKSEYNVEAIYESVNVATARWVESTDVK
CCCCEEEEHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHH
KFEEFKRKNEVQLALDGGDNLTYIAPTMVNLNLTQERYPDVVFRKTREH
HHHHHHCCCCEEEEEECCCCEEEEECEEEEEEECHHHCCCHHHHHCCCC
>Mature Secondary Structure 
TLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSSQHAKSDWM
CCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
EMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVE
HHHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
DRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENELKIGCAPITWPIGCGKLFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHH
GVYHLYKDETYLYQTGKGHTIQEVRIVKGLNNPDLDAAVGEDLAQQLRDELELVQGASNE
HHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FDKDLFLAGEITPVFFGTALGNFGVDHMLDGLVEWAPAPMPRNTDTREVTATEEKFTGFV
CCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHCEEE
FKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVRIGKDVVISDALTFMAGDRSHVEEAY
EEEEECCCCCHHHHHHHHHHHHCHHHCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHHCC
PGDIIGLHNHGTIQIGDTFTQGEMMKFTGIPNFAPELFRRIRLKDPLKQKQLLKGLVQLS
CCCEEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHC
EEGAVQVFRPIANNDLIVGAVGVLQFDVVVARLKSEYNVEAIYESVNVATARWVESTDVK
CCCCEEEEHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHH
KFEEFKRKNEVQLALDGGDNLTYIAPTMVNLNLTQERYPDVVFRKTREH
HHHHHHCCCCEEEEEECCCCEEEEECEEEEEEECHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA