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The map label for this gene is pmbA [H]

Identifier: 238892714

GI number: 238892714

Start: 501866

End: 503218

Strand: Direct

Name: pmbA [H]

Synonym: KP1_0505

Alternate gene names: 238892714

Gene position: 501866-503218 (Clockwise)

Preceding gene: 238892711

Following gene: 238892715

Centisome position: 9.56

GC content: 59.28

Gene sequence:

>1353_bases
ATGGCATTAGCAATGAAAGTCATCTCTCAAGTTGCACAGCAGCGTAAGACGCTGGAAGAAGCCGTTACCACGGCACTGGA
ACTGGCGGCAGGTAAATCTGATGGTGCGGAAGTTTCCGTCAGCAAAACCACGGGCATCGGCGTCAGCACCCGCTATGGCG
AAGTGGAGAATGTAGAATTCAACAGCGATGGGGCATTAGGCATCACGGTTTACCATCAGAACCGCAAGGGCAGCGCCTCC
TCCACCGATCTTAGCCCGGACGCCATCGCCCGCACCGTGCAGGCGGCGCTGGATATCGCCCGCTATACCTCCCCGGACCC
GTTTGCCGGCGTAGCCGATAAAGATCTGCTGGCCTTTGACGCCCCGGATCTGGACCTGTTCCACCCGGCGGAGGTCTCCC
CGGACGACGCCATCGAACTGGCCGCCCGCGCGGAGCAAGCGGCCCTGAAGGCCGATAAACGCATCACCAACACCGAAGGC
GGCAGCTTTAACAGCCACTACGGCATTAAAGTGTTCGGCAACAGCCATGGCATGCTGCAGAGCTACTGCTCGACTCGCCA
CTCCCTCTCCAGCTGCGTCATTGCGGAAGATGACGGCGATATGGAGCGCGACTACGCCTACACCATTGGCCGGGCGATCG
AGGATCTGCAGTCCCCGGAATGGGTCGGGGAAGAGTGCGCCCGCCGCACCCTGTCGCGCCTGGCGCCGCGTAAGCTGTCG
ACCATGAAGGCGCCGGTCATTTTCGCCAACGAAGTGGCGACCGGGTTGTTTGGTCACCTGGTCGGAGCGATTGCCGGCGG
CGCGGTCTACCGTAAATCGACCTTCCTGCTCGATTCGCTGGGCAAACAAATTTTGCCGGAGTGGCTGACCATTGAAGAGC
ATCCGCACCTGCTGAAAGGGCTGGCCTCCACGCCGTTCGACAGCGAAGGCGTGCGCACCGAACGTCGCGATATCGTCAAA
GACGGCGTGCTGACCCAGTGGCTGCTGACCAATTACTCAGCGCGCAAGCTGGGGATGAAAAGCACCGGCCACGCCGGGGG
CATCCACAACTGGCGCATTAACGGCCGCGGCCTGAGTTTTGCAAAGATGCTTAAAGAGATGGGCACCGGTCTTGTGGTTA
CCGAACTGATGGGGCAGGGTGTCAGTGGCGTCACCGGCGACTACTCACGCGGCGCATCCGGCTTCTGGGTAGAAAACGGC
GAAATTCAATATCCTGTCAGTGAGATCACCATTGCAGGAAATCTGAAAGAAATGTGGCGTAATATCGTGACCATTGGCGA
TGATATTGAAACGCGCAGCAACATTCAGTGCGGTTCTGTGCTGCTGCCGGAGATGAAAATCGCCGGGCAATAA

Upstream 100 bases:

>100_bases
GTCATCTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGGCGCGCACTGAAAAACGGTTCTCTGTTAGACTTC
AGTTAACTCCTTCTTACATT

Downstream 100 bases:

>100_bases
CACCTGTTTGCTTAAATAATAAAAAGGAAGTGAGCAATGCGTAAAAAACTGTTAGCGATGCTGGCTGTCTCTGCTTTTGC
GCTCGGTTCAGCGTCGGCCT

Product: peptidase PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MALAMKVISQVAQQRKTLEEAVTTALELAAGKSDGAEVSVSKTTGIGVSTRYGEVENVEFNSDGALGITVYHQNRKGSAS
STDLSPDAIARTVQAALDIARYTSPDPFAGVADKDLLAFDAPDLDLFHPAEVSPDDAIELAARAEQAALKADKRITNTEG
GSFNSHYGIKVFGNSHGMLQSYCSTRHSLSSCVIAEDDGDMERDYAYTIGRAIEDLQSPEWVGEECARRTLSRLAPRKLS
TMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVK
DGVLTQWLLTNYSARKLGMKSTGHAGGIHNWRINGRGLSFAKMLKEMGTGLVVTELMGQGVSGVTGDYSRGASGFWVENG
EIQYPVSEITIAGNLKEMWRNIVTIGDDIETRSNIQCGSVLLPEMKIAGQ

Sequences:

>Translated_450_residues
MALAMKVISQVAQQRKTLEEAVTTALELAAGKSDGAEVSVSKTTGIGVSTRYGEVENVEFNSDGALGITVYHQNRKGSAS
STDLSPDAIARTVQAALDIARYTSPDPFAGVADKDLLAFDAPDLDLFHPAEVSPDDAIELAARAEQAALKADKRITNTEG
GSFNSHYGIKVFGNSHGMLQSYCSTRHSLSSCVIAEDDGDMERDYAYTIGRAIEDLQSPEWVGEECARRTLSRLAPRKLS
TMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVK
DGVLTQWLLTNYSARKLGMKSTGHAGGIHNWRINGRGLSFAKMLKEMGTGLVVTELMGQGVSGVTGDYSRGASGFWVENG
EIQYPVSEITIAGNLKEMWRNIVTIGDDIETRSNIQCGSVLLPEMKIAGQ
>Mature_449_residues
ALAMKVISQVAQQRKTLEEAVTTALELAAGKSDGAEVSVSKTTGIGVSTRYGEVENVEFNSDGALGITVYHQNRKGSASS
TDLSPDAIARTVQAALDIARYTSPDPFAGVADKDLLAFDAPDLDLFHPAEVSPDDAIELAARAEQAALKADKRITNTEGG
SFNSHYGIKVFGNSHGMLQSYCSTRHSLSSCVIAEDDGDMERDYAYTIGRAIEDLQSPEWVGEECARRTLSRLAPRKLST
MKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKD
GVLTQWLLTNYSARKLGMKSTGHAGGIHNWRINGRGLSFAKMLKEMGTGLVVTELMGQGVSGVTGDYSRGASGFWVENGE
IQYPVSEITIAGNLKEMWRNIVTIGDDIETRSNIQCGSVLLPEMKIAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=450, Percent_Identity=91.5555555555556, Blast_Score=867, Evalue=0.0,
Organism=Escherichia coli, GI1789640, Length=434, Percent_Identity=20.2764976958525, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48486; Mature: 48355

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAMKVISQVAQQRKTLEEAVTTALELAAGKSDGAEVSVSKTTGIGVSTRYGEVENVEF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEECCCCCCCEEE
NSDGALGITVYHQNRKGSASSTDLSPDAIARTVQAALDIARYTSPDPFAGVADKDLLAFD
CCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEC
APDLDLFHPAEVSPDDAIELAARAEQAALKADKRITNTEGGSFNSHYGIKVFGNSHGMLQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHH
SYCSTRHSLSSCVIAEDDGDMERDYAYTIGRAIEDLQSPEWVGEECARRTLSRLAPRKLS
HHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHH
TMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKG
HHCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHH
LASTPFDSEGVRTERRDIVKDGVLTQWLLTNYSARKLGMKSTGHAGGIHNWRINGRGLSF
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCEEEECCCCCHH
AKMLKEMGTGLVVTELMGQGVSGVTGDYSRGASGFWVENGEIQYPVSEITIAGNLKEMWR
HHHHHHHCCCEEEHHHHCCCCCCCCCCCCCCCCCEEEECCEEECCHHHEEEECCHHHHHH
NIVTIGDDIETRSNIQCGSVLLPEMKIAGQ
HHEECCCCCCCCCCCCCCCEECCCHHCCCC
>Mature Secondary Structure 
ALAMKVISQVAQQRKTLEEAVTTALELAAGKSDGAEVSVSKTTGIGVSTRYGEVENVEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEECCCCCCCEEE
NSDGALGITVYHQNRKGSASSTDLSPDAIARTVQAALDIARYTSPDPFAGVADKDLLAFD
CCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEC
APDLDLFHPAEVSPDDAIELAARAEQAALKADKRITNTEGGSFNSHYGIKVFGNSHGMLQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHH
SYCSTRHSLSSCVIAEDDGDMERDYAYTIGRAIEDLQSPEWVGEECARRTLSRLAPRKLS
HHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHH
TMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKG
HHCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHH
LASTPFDSEGVRTERRDIVKDGVLTQWLLTNYSARKLGMKSTGHAGGIHNWRINGRGLSF
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCEEEECCCCCHH
AKMLKEMGTGLVVTELMGQGVSGVTGDYSRGASGFWVENGEIQYPVSEITIAGNLKEMWR
HHHHHHHCCCEEEHHHHCCCCCCCCCCCCCCCCCEEEECCEEECCHHHEEEECCHHHHHH
NIVTIGDDIETRSNIQCGSVLLPEMKIAGQ
HHEECCCCCCCCCCCCCCCEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]