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The map label for this gene is sgaE [H]

Identifier: 238892677

GI number: 238892677

Start: 465609

End: 466295

Strand: Direct

Name: sgaE [H]

Synonym: KP1_0465

Alternate gene names: 238892677

Gene position: 465609-466295 (Clockwise)

Preceding gene: 238892676

Following gene: 238892680

Centisome position: 8.87

GC content: 61.43

Gene sequence:

>687_bases
ATGCAACAGCTGAAACAGCAGGTGTTCGAGGCCAATATGGATCTGCCGCGCTACGGCCTGGTGACCTTTACCTGGGGTAA
CGTTAGCGCCATTGACCGGCAGCGTGGGCTGGTGGTGATTAAGCCCAGCGGCATCGCCTACGAGTCAATGACCGTCGATG
ACATGTCGGTGGTTGATCTGCAGGGGCACGTGGTGGAAGGGCGCTGGCGTCCCTCCTCTGATACCGCCACCCATCTGGCG
CTCTATCGCCGTTATCCCGACCTTGGCGGGGTGGTGCATACCCATTCGACGCACGCCACCGCCTGGGCGCAGGCGGGGCT
GGCGATCCCGGCGCTCGGCACCACCCATGCGGACTACTTCTTCGGCGATATTCCCTGCACCCGGGCGCTTAGCGCACAGG
AGGTGGACGAGGCCTACGAGCTGAACACCGGGCAGGTGATTATCGAAACGCTGGGTGAGGCCAATCCGCTGCATACGCCG
GGGATCGTCGTCTATCAGCACGGGCCATTTGCCTGGGGCAAAGACGCCCACGAGGCGGTACACAACGCGGTGGTGATGGA
AGAGGTGGCGCGCATGGCGTGGATTGCCCGCGGCATTAATCCGCAGTTGCAGCCCATCGATAGCTGGCTGATGAATAAGC
ATTTTCAGCGTAAGCATGGGCCAAACGCCTACTATGGGCAAAAGTAG

Upstream 100 bases:

>100_bases
TGTGGAGCGAAACCAGCGCTGACCCGCTGGCGGAGGTGGCGAAGGCGCGTGACTGGGTGAAGGCGCGGATGGCCCGCGCG
GGCCTGATGGAGGCGGCATA

Downstream 100 bases:

>100_bases
ATAGCGCGCTCCGGGGCTTTCACCCCTCACGGTGAAGCAACTGGTCTGGCCCCGGAGCCGTTTTTAATCCTGACGATAGG
CCGACGGCATCACGCGCATG

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: L-ascorbate utilization protein F; Phosphoribulose isomerase [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MQQLKQQVFEANMDLPRYGLVTFTWGNVSAIDRQRGLVVIKPSGIAYESMTVDDMSVVDLQGHVVEGRWRPSSDTATHLA
LYRRYPDLGGVVHTHSTHATAWAQAGLAIPALGTTHADYFFGDIPCTRALSAQEVDEAYELNTGQVIIETLGEANPLHTP
GIVVYQHGPFAWGKDAHEAVHNAVVMEEVARMAWIARGINPQLQPIDSWLMNKHFQRKHGPNAYYGQK

Sequences:

>Translated_228_residues
MQQLKQQVFEANMDLPRYGLVTFTWGNVSAIDRQRGLVVIKPSGIAYESMTVDDMSVVDLQGHVVEGRWRPSSDTATHLA
LYRRYPDLGGVVHTHSTHATAWAQAGLAIPALGTTHADYFFGDIPCTRALSAQEVDEAYELNTGQVIIETLGEANPLHTP
GIVVYQHGPFAWGKDAHEAVHNAVVMEEVARMAWIARGINPQLQPIDSWLMNKHFQRKHGPNAYYGQK
>Mature_228_residues
MQQLKQQVFEANMDLPRYGLVTFTWGNVSAIDRQRGLVVIKPSGIAYESMTVDDMSVVDLQGHVVEGRWRPSSDTATHLA
LYRRYPDLGGVVHTHSTHATAWAQAGLAIPALGTTHADYFFGDIPCTRALSAQEVDEAYELNTGQVIIETLGEANPLHTP
GIVVYQHGPFAWGKDAHEAVHNAVVMEEVARMAWIARGINPQLQPIDSWLMNKHFQRKHGPNAYYGQK

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790642, Length=228, Percent_Identity=85.9649122807018, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1790008, Length=230, Percent_Identity=59.5652173913044, Blast_Score=286, Evalue=7e-79,
Organism=Escherichia coli, GI1786247, Length=230, Percent_Identity=57.3913043478261, Blast_Score=281, Evalue=3e-77,
Organism=Escherichia coli, GI1789164, Length=137, Percent_Identity=35.7664233576642, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 25342; Mature: 25342

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQLKQQVFEANMDLPRYGLVTFTWGNVSAIDRQRGLVVIKPSGIAYESMTVDDMSVVDL
CHHHHHHHHHCCCCCCCCCEEEEECCCEEEHHCCCCEEEECCCCCEEEEEECCCCEEEEE
QGHVVEGRWRPSSDTATHLALYRRYPDLGGVVHTHSTHATAWAQAGLAIPALGTTHADYF
CCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCEECCCCCCCCCCEE
FGDIPCTRALSAQEVDEAYELNTGQVIIETLGEANPLHTPGIVVYQHGPFAWGKDAHEAV
ECCCCHHHCCCHHHHHHHHCCCCCHHHEEHHCCCCCCCCCCEEEEECCCCCCCCHHHHHH
HNAVVMEEVARMAWIARGINPQLQPIDSWLMNKHFQRKHGPNAYYGQK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQQLKQQVFEANMDLPRYGLVTFTWGNVSAIDRQRGLVVIKPSGIAYESMTVDDMSVVDL
CHHHHHHHHHCCCCCCCCCEEEEECCCEEEHHCCCCEEEECCCCCEEEEEECCCCEEEEE
QGHVVEGRWRPSSDTATHLALYRRYPDLGGVVHTHSTHATAWAQAGLAIPALGTTHADYF
CCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCEECCCCCCCCCCEE
FGDIPCTRALSAQEVDEAYELNTGQVIIETLGEANPLHTPGIVVYQHGPFAWGKDAHEAV
ECCCCHHHCCCHHHHHHHHCCCCCHHHEEHHCCCCCCCCCCEEEEECCCCCCCCHHHHHH
HNAVVMEEVARMAWIARGINPQLQPIDSWLMNKHFQRKHGPNAYYGQK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA