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The map label for this gene is miaA [H]

Identifier: 238892655

GI number: 238892655

Start: 445105

End: 446055

Strand: Direct

Name: miaA [H]

Synonym: KP1_0439

Alternate gene names: 238892655

Gene position: 445105-446055 (Clockwise)

Preceding gene: 238892654

Following gene: 238892656

Centisome position: 8.48

GC content: 54.26

Gene sequence:

>951_bases
ATGAATGATGTAACCGAGGCGAGCCTGCCCAAGGCAATTTTTTTAATGGGCCCGACGGCCTCCGGCAAAACCGCGCTGGC
GATAGCCTTACGTAAAGTTTTGCCAGTAGAGTTGATTAGCGTTGATTCCGCCCTCATTTATCGGGGAATGGATATTGGCA
CCGCCAAACCGGATGCTGCGGAGCTTAGCGCTGCGCCGCATCGGCTGCTGGATATTTTGGATCCTGCGGAGGCCTATTCC
GCGGCGGATTTTCGCCGCGATGCGCTGGCGGCGATGGCGGATATTGTCGCCGCCGGGCGAATTCCGCTGTTAGTCGGCGG
AACGATGTTGTATTTCAAAGCGTTGCTGGAAGGATTGTCGCCCTTACCGTCGGCTGACCCAGAGGTCAGAGCGCGGATTG
AGCAGCAGGCCGCAGAGCAGGGGTGGAATGCGTTACATCAGCAATTACAGGAGATCGATCCCGTGGCTGCTGCGCGTATT
CATCCAAATGATCCGCAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGCA
AACGTCAGGAGACGCTCTGCCATATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCTCCATCAACGCA
TAGAACAGCGTTTTCATCAGATGTTAGCTTCAGGTTTTGAAGCAGAAGTACGGGCGCTTTTTGCCCGCGGAGATTTGCAT
ACGGACATGCCTTCCATCCGTTGTGTCGGATACCGCCAGATGTGGTCATATCTCAATGGCGAGATCCCGTATGATGAGAT
GGTTTATCGAGGTGTTTGCGCCACGAGACAGTTAGCCAAACGTCAGGTCACCTGGCTGCGCGGTTGGGAAGGTGTGCACT
GGTTAGACAGTGAACAACCTGAACAAGCGCTCAACAAAGTATTACAGGTTGTTGGTGCGAGCCAGAACTGA

Upstream 100 bases:

>100_bases
CTGGCGGACGTTGAGCGTCTTTGCCCGCAGCTGTTGAGAACGCCGCCGGGTGGCCTGTTACAACCTGTAGATCTCCATTC
GGCGATGAATGCCCTGAAAG

Downstream 100 bases:

>100_bases
ACGTGTACAATTAAACGGTATCGTGCGTAATTTTTTTTGAGAATCGTAAGGTTCTGAGTACAAAACAAGCATACATATAA
GGAAAAGAGAGAATGGCTAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MNDVTEASLPKAIFLMGPTASGKTALAIALRKVLPVELISVDSALIYRGMDIGTAKPDAAELSAAPHRLLDILDPAEAYS
AADFRRDALAAMADIVAAGRIPLLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWNALHQQLQEIDPVAAARI
HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRFHQMLASGFEAEVRALFARGDLH
TDMPSIRCVGYRQMWSYLNGEIPYDEMVYRGVCATRQLAKRQVTWLRGWEGVHWLDSEQPEQALNKVLQVVGASQN

Sequences:

>Translated_316_residues
MNDVTEASLPKAIFLMGPTASGKTALAIALRKVLPVELISVDSALIYRGMDIGTAKPDAAELSAAPHRLLDILDPAEAYS
AADFRRDALAAMADIVAAGRIPLLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWNALHQQLQEIDPVAAARI
HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRFHQMLASGFEAEVRALFARGDLH
TDMPSIRCVGYRQMWSYLNGEIPYDEMVYRGVCATRQLAKRQVTWLRGWEGVHWLDSEQPEQALNKVLQVVGASQN
>Mature_316_residues
MNDVTEASLPKAIFLMGPTASGKTALAIALRKVLPVELISVDSALIYRGMDIGTAKPDAAELSAAPHRLLDILDPAEAYS
AADFRRDALAAMADIVAAGRIPLLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWNALHQQLQEIDPVAAARI
HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRFHQMLASGFEAEVRALFARGDLH
TDMPSIRCVGYRQMWSYLNGEIPYDEMVYRGVCATRQLAKRQVTWLRGWEGVHWLDSEQPEQALNKVLQVVGASQN

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=307, Percent_Identity=28.3387622149837, Blast_Score=121, Evalue=7e-28,
Organism=Escherichia coli, GI1790613, Length=313, Percent_Identity=91.3738019169329, Blast_Score=595, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI25144712, Length=269, Percent_Identity=28.2527881040892, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=27.3356401384083, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24649830, Length=273, Percent_Identity=31.5018315018315, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 34845; Mature: 34845

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVTEASLPKAIFLMGPTASGKTALAIALRKVLPVELISVDSALIYRGMDIGTAKPDAA
CCCCCHHCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHH
ELSAAPHRLLDILDPAEAYSAADFRRDALAAMADIVAAGRIPLLVGGTMLYFKALLEGLS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
PLPSADPEVRARIEQQAAEQGWNALHQQLQEIDPVAAARIHPNDPQRLSRALEVFFISGK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHCCCC
TLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRFHQMLASGFEAEVRALFARGDLH
HHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
TDMPSIRCVGYRQMWSYLNGEIPYDEMVYRGVCATRQLAKRQVTWLRGWEGVHWLDSEQP
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
EQALNKVLQVVGASQN
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDVTEASLPKAIFLMGPTASGKTALAIALRKVLPVELISVDSALIYRGMDIGTAKPDAA
CCCCCHHCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHH
ELSAAPHRLLDILDPAEAYSAADFRRDALAAMADIVAAGRIPLLVGGTMLYFKALLEGLS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
PLPSADPEVRARIEQQAAEQGWNALHQQLQEIDPVAAARIHPNDPQRLSRALEVFFISGK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHCCCC
TLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRFHQMLASGFEAEVRALFARGDLH
HHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
TDMPSIRCVGYRQMWSYLNGEIPYDEMVYRGVCATRQLAKRQVTWLRGWEGVHWLDSEQP
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
EQALNKVLQVVGASQN
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA