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The map label for this gene is purH [H]

Identifier: 238892482

GI number: 238892482

Start: 255302

End: 256891

Strand: Reverse

Name: purH [H]

Synonym: KP1_0248

Alternate gene names: 238892482

Gene position: 256891-255302 (Counterclockwise)

Preceding gene: 238892483

Following gene: 238892481

Centisome position: 4.89

GC content: 58.11

Gene sequence:

>1590_bases
ATGCAACAACGTCGTCCAGTCCGCCGCGCTCTGCTCAGTGTTTCTGACAAGGCCGGTATCGTCGAATTCGCGCAGGCGCT
TTCCGCTCGTGGTGTCGAGCTGCTGTCCACCGGCGGCACCGCTCGCCTGCTGGCAGATAAAGGCCTGCCGGTTACCGAAG
TCTCTGACTACACCGGTTTCCCGGAAATGATGGATGGACGCGTGAAGACCCTGCATCCGAAAGTACACGGCGGCATCCTC
GGTCGTCGCGGTCAGGATGACGGTATCATGCAGCAACACGGTATCGCGCCTATCGATATGGTTGTCGTGAACCTTTACCC
GTTCGCCCAGACCGTCGCTCGTGAAGGGTGCTCGCTGGAAGACGCGGTGGAAAACATCGATATCGGCGGCCCGACTATGG
TGCGCTCTGCGGCGAAGAACCATAAAGATGTCGCCATCGTAGTAAAGAGCAGCGACTACGACGCCATCATTAATGAGATG
GACGCCAACGAAGGTTCCCTCACGCTGGCCACCCGCTTCGACCTCGCCATTAAAGCCTTCGAACACACCGCCGCTTACGA
CAGCATGATCGCCAACTACTTCGGCAGCATGGTTCCGGCCTATCACGGCGAAAGCAAAGAAGCCGCTGGCCGCTTCCCGC
GTACCCTGAATCTGAACTTCATTAAGAAGCAGGATATGCGCTACGGCGAAAACAGCCACCAGCAGGCGGCTTTCTATATA
GAAGAGAATGTAAAAGAGGCCTCCGTGGCGACCGCCACTCAGCTGCAGGGCAAAGCGCTCTCCTATAACAACATTGCCGA
TACCGATGCGGCGCTGGAGTGTGTAAAAGAGTTCAACGAGCCGGCCTGCGTTATCGTTAAGCACGCCAACCCGTGCGGCG
TTGCCGTCAGCGACTCGATTCTTGACGCCTACGATCGCGCTTACAAAACCGACCCGACCTCCGCGTTCGGCGGCATCATT
GCCTTTAACCGCGAGCTGGATGCTGAAACCGCGCAGGCGATCATCTCTCGCCAGTTCGTCGAAGTGATCATCGCGCCGTC
CGCCAGCGAAGAAGCGCTGAAAATCACCGCCGCGAAGCAGAACGTCCGCGTACTGACCTGCGGCCAGTGGGATACTCGCG
TTGCCGGCCTCGATTTCAAACGCGTTAACGGTGGTCTGCTGGTTCAGGACCGCGATCTCGGCATGGTCACCGCAGGTGAA
CTGCGCGTGGTCAGCAAACGTCAGCCAACCGAGCAGGAACTGCGCGATGCGCTGTTCTGCTGGAAAGTGGCGAAATTCGT
CAAATCCAACGCCATTGTGTATGCCAAAGATAATATGACCATCGGTATAGGCGCCGGTCAGATGAGCCGCGTCTACTCGG
CGAAAATCGCCGGAATTAAAGCCGGTGATGAAGGTCTGGAAGTGAAAGGTTCCGCCATGGCCTCTGACGCTTTCTTCCCG
TTCCGCGACGGCATCGATGCCGCAGCCGCGGTAGGCATCACCTGCGTTATTCAGCCGGGCGGCTCTATCCGTGACGACGA
AGTGATTGCCGCCGCTGACGAACACGGCATCGCCATGATCTTCACCGACATGCGCCACTTCCGCCATTAA

Upstream 100 bases:

>100_bases
CGCTATGACACGGATGCCCGGTTGGGTGCGTTAGGTGAGTTTTTAGGCAAAAACTCACCTAACGCTCTCTGTAATGTTCA
AATCCTGGGGATTTAATACC

Downstream 100 bases:

>100_bases
TTCCTGGAGCAGCAGATGAAAGTATTAGTAATCGGTAACGGCGGGCGCGAGCACGCGCTGGCCTGGAAAGCGGCTCAGTC
GCCGCTGGTTGATACCGTCT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLADKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDGIMQQHGIAPIDMVVVNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIINEM
DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EENVKEASVATATQLQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVAVSDSILDAYDRAYKTDPTSAFGGII
AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWDTRVAGLDFKRVNGGLLVQDRDLGMVTAGE
LRVVSKRQPTEQELRDALFCWKVAKFVKSNAIVYAKDNMTIGIGAGQMSRVYSAKIAGIKAGDEGLEVKGSAMASDAFFP
FRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHGIAMIFTDMRHFRH

Sequences:

>Translated_529_residues
MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLADKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDGIMQQHGIAPIDMVVVNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIINEM
DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EENVKEASVATATQLQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVAVSDSILDAYDRAYKTDPTSAFGGII
AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWDTRVAGLDFKRVNGGLLVQDRDLGMVTAGE
LRVVSKRQPTEQELRDALFCWKVAKFVKSNAIVYAKDNMTIGIGAGQMSRVYSAKIAGIKAGDEGLEVKGSAMASDAFFP
FRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHGIAMIFTDMRHFRH
>Mature_529_residues
MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLADKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDGIMQQHGIAPIDMVVVNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIINEM
DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EENVKEASVATATQLQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVAVSDSILDAYDRAYKTDPTSAFGGII
AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWDTRVAGLDFKRVNGGLLVQDRDLGMVTAGE
LRVVSKRQPTEQELRDALFCWKVAKFVKSNAIVYAKDNMTIGIGAGQMSRVYSAKIAGIKAGDEGLEVKGSAMASDAFFP
FRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHGIAMIFTDMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=486, Percent_Identity=39.5061728395062, Blast_Score=275, Evalue=9e-74,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=94.8960302457467, Blast_Score=1031, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=614, Percent_Identity=33.0618892508143, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI71985574, Length=352, Percent_Identity=26.4204545454545, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=115, Percent_Identity=37.3913043478261, Blast_Score=78, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323768, Length=488, Percent_Identity=35.8606557377049, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6323056, Length=491, Percent_Identity=35.4378818737271, Blast_Score=262, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24649832, Length=487, Percent_Identity=37.5770020533881, Blast_Score=268, Evalue=8e-72,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57192; Mature: 57192

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLADKGLPVTEVSDYTGF
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCCCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDGIMQQHGIAPIDMVVVNLYPFAQTVAREGCSLE
HHHHCCCEEEECCHHHCCCCCCCCCCCCCHHHCCCCCHHEEEEEHHHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIINEMDANEGSLTLATRFDLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEEEHHHHHHHH
EHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEECCCCCCCCCCCCEEEEEE
EENVKEASVATATQLQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVAVSDSI
ECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEECHHH
LDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQ
HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECC
NVRVLTCGQWDTRVAGLDFKRVNGGLLVQDRDLGMVTAGELRVVSKRQPTEQELRDALFC
CEEEEEECCCCCEEECCEEEECCCCEEEEECCCCEEECCCEEEECCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYAKDNMTIGIGAGQMSRVYSAKIAGIKAGDEGLEVKGSAMASDAFFP
HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHEEEEECCCCCCEECCCEEECCCCCC
FRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHGIAMIFTDMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEEECCCCEEEEEECHHHHCC
>Mature Secondary Structure
MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLADKGLPVTEVSDYTGF
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCCCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDGIMQQHGIAPIDMVVVNLYPFAQTVAREGCSLE
HHHHCCCEEEECCHHHCCCCCCCCCCCCCHHHCCCCCHHEEEEEHHHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIINEMDANEGSLTLATRFDLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEEEHHHHHHHH
EHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEECCCCCCCCCCCCEEEEEE
EENVKEASVATATQLQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVAVSDSI
ECCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEECHHH
LDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQ
HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECC
NVRVLTCGQWDTRVAGLDFKRVNGGLLVQDRDLGMVTAGELRVVSKRQPTEQELRDALFC
CEEEEEECCCCCEEECCEEEECCCCEEEEECCCCEEECCCEEEECCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYAKDNMTIGIGAGQMSRVYSAKIAGIKAGDEGLEVKGSAMASDAFFP
HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHEEEEECCCCCCEECCCEEECCCCCC
FRDGIDAAAAVGITCVIQPGGSIRDDEVIAAADEHGIAMIFTDMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEEECCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA