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The map label for this gene is purD [H]

Identifier: 238892481

GI number: 238892481

Start: 253991

End: 255286

Strand: Reverse

Name: purD [H]

Synonym: KP1_0247

Alternate gene names: 238892481

Gene position: 255286-253991 (Counterclockwise)

Preceding gene: 238892482

Following gene: 238892478

Centisome position: 4.86

GC content: 62.5

Gene sequence:

>1296_bases
ATGAAAGTATTAGTAATCGGTAACGGCGGGCGCGAGCACGCGCTGGCCTGGAAAGCGGCTCAGTCGCCGCTGGTTGATAC
CGTCTACGTCGCGCCGGGCAACGCCGGTACCGCCCTGGAGCCGTCGCTGCAAAACGTCGCCATCGGCGTCACCGATATTC
CGGCGCTGCTTCGCTTCGCGCAGGATGAGAACATCGATCTGACCATCGTTGGTCCGGAAGCGCCGTTGGTCATTGGCGTG
GTCGACGCGTTCCGTGCCGCCGGGCTGAACATCTTCGGGCCAACCGAAGGCGCCGCGCAGCTGGAAGGCTCAAAAGCCTT
CACCAAAGATTTTCTCGCCCGTCATCATATTCCGACGGCGGAATACCAGAACTTCACCGAAGTGGAGCCTGCGCTGGCTT
ATCTGCGCGAGAAAGGCGCGCCGATCGTCATCAAAGCCGACGGTCTGGCGGCGGGTAAAGGCGTTATCGTGGCGATGACC
CTCGAGGAAGCCGAAGCGGCGGTCAAAGATATGTTGGCCGGCAACGCCTTTGGCGACGCAGGCCACCGCATCGTGATTGA
AGAGTTCCTCGATGGCGAAGAGGCTAGCTTTATTGTGATGGTCGACGGCGAGCACGTCCTGCCGATGGCCACCAGCCAGG
ATCACAAGCGCGTCGGCAACGGCGATACCGGCCCGAACACCGGCGGGATGGGGGCGTACTCCCCGGCGCCGGTGGTTACT
GACGAAGTCCACCAGCGCACCATGGAGCGGATTATCTGGCCAACCGTGAAAGGCATGGCCGCTGAAGGCAACACCTATAC
CGGTTTCCTCTACGCCGGTCTGATGATCGACAAGCAGGGCAACCCGAAGGTGATTGAGTTCAACTGCCGCTTTGGCGACC
CGGAAACCCAACCGATCATGCTGCGCATGAAGTCCGACCTGGTCGAGCTGTGCCTCGCCGCCTGCGCGGGCAAGCTGGAC
GAGAAAACCTCCGAGTGGGATGACCGCGCGTCGCTGGGCGTGGTGGTTGCCGCCGGCGGCTATCCGGGCAGCTACAATAC
GGGCGATGAGATCTACGGCCTGCCGCAGCAGGAAGTCGCAGACGGTAAAGTCTTCCACGCCGGCACCAAACTGTCTGACG
ACCAGCGCGTGGTCACCAACGGCGGGCGCGTGCTGTGCGTCACCGCGCTGGGTGACTCCGTAGCGCAGGCGCAGCAGCGC
GCCTATCAGCTGCTGACCGACATTCGCTGGGACGGTAGCTTTAGCCGTAGCGATATCGGCTGGCGCGCCATCGAACGTGA
AAAGGCTAACGGCTAA

Upstream 100 bases:

>100_bases
TATCCGTGACGACGAAGTGATTGCCGCCGCTGACGAACACGGCATCGCCATGATCTTCACCGACATGCGCCACTTCCGCC
ATTAATTCCTGGAGCAGCAG

Downstream 100 bases:

>100_bases
GTTTCGCCAGCAGCGTTTTGCGCGTAATGCCTAACTGACGGGCGGCTTCGGTTTTGTTGCCGCCCGTTTTTTCCAGCGCC
GCCAGAATCACCTCTTTCTC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKVLVIGNGGREHALAWKAAQSPLVDTVYVAPGNAGTALEPSLQNVAIGVTDIPALLRFAQDENIDLTIVGPEAPLVIGV
VDAFRAAGLNIFGPTEGAAQLEGSKAFTKDFLARHHIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
LEEAEAAVKDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGNGDTGPNTGGMGAYSPAPVVT
DEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMIDKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACAGKLD
EKTSEWDDRASLGVVVAAGGYPGSYNTGDEIYGLPQQEVADGKVFHAGTKLSDDQRVVTNGGRVLCVTALGDSVAQAQQR
AYQLLTDIRWDGSFSRSDIGWRAIEREKANG

Sequences:

>Translated_431_residues
MKVLVIGNGGREHALAWKAAQSPLVDTVYVAPGNAGTALEPSLQNVAIGVTDIPALLRFAQDENIDLTIVGPEAPLVIGV
VDAFRAAGLNIFGPTEGAAQLEGSKAFTKDFLARHHIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
LEEAEAAVKDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGNGDTGPNTGGMGAYSPAPVVT
DEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMIDKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACAGKLD
EKTSEWDDRASLGVVVAAGGYPGSYNTGDEIYGLPQQEVADGKVFHAGTKLSDDQRVVTNGGRVLCVTALGDSVAQAQQR
AYQLLTDIRWDGSFSRSDIGWRAIEREKANG
>Mature_431_residues
MKVLVIGNGGREHALAWKAAQSPLVDTVYVAPGNAGTALEPSLQNVAIGVTDIPALLRFAQDENIDLTIVGPEAPLVIGV
VDAFRAAGLNIFGPTEGAAQLEGSKAFTKDFLARHHIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
LEEAEAAVKDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGNGDTGPNTGGMGAYSPAPVVT
DEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMIDKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACAGKLD
EKTSEWDDRASLGVVVAAGGYPGSYNTGDEIYGLPQQEVADGKVFHAGTKLSDDQRVVTNGGRVLCVTALGDSVAQAQQR
AYQLLTDIRWDGSFSRSDIGWRAIEREKANG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=50.5882352941176, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=50.5882352941176, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=50.5882352941176, Blast_Score=414, Evalue=1e-115,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=50.5882352941176, Blast_Score=414, Evalue=1e-115,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=90.4205607476635, Blast_Score=795, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=427, Percent_Identity=41.6861826697892, Blast_Score=303, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=42.4719101123596, Blast_Score=345, Evalue=1e-95,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=43.8515081206497, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI24582400, Length=436, Percent_Identity=43.5779816513761, Blast_Score=341, Evalue=5e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45912; Mature: 45912

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVIGNGGREHALAWKAAQSPLVDTVYVAPGNAGTALEPSLQNVAIGVTDIPALLRFA
CEEEEEECCCCCCCEEEHHCCCCCEEEEEEECCCCCCCCCCCHHHEEEEHHHHHHHHHHH
QDENIDLTIVGPEAPLVIGVVDAFRAAGLNIFGPTEGAAQLEGSKAFTKDFLARHHIPTA
CCCCEEEEEECCCCCEEEEEHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCH
EYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMTLEEAEAAVKDMLAGNAFGDA
HCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGNGDTGPNTGGMGAYSPAPVVT
CCEEEEHHHCCCCCCEEEEEECCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCH
DEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMIDKQGNPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCEECCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCEEE
LRMKSDLVELCLAACAGKLDEKTSEWDDRASLGVVVAAGGYPGSYNTGDEIYGLPQQEVA
EEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEECCCHHHHC
DGKVFHAGTKLSDDQRVVTNGGRVLCVTALGDSVAQAQQRAYQLLTDIRWDGSFSRSDIG
CCEEEECCCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
WRAIEREKANG
HHHHHHHCCCC
>Mature Secondary Structure
MKVLVIGNGGREHALAWKAAQSPLVDTVYVAPGNAGTALEPSLQNVAIGVTDIPALLRFA
CEEEEEECCCCCCCEEEHHCCCCCEEEEEEECCCCCCCCCCCHHHEEEEHHHHHHHHHHH
QDENIDLTIVGPEAPLVIGVVDAFRAAGLNIFGPTEGAAQLEGSKAFTKDFLARHHIPTA
CCCCEEEEEECCCCCEEEEEHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCH
EYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMTLEEAEAAVKDMLAGNAFGDA
HCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRVGNGDTGPNTGGMGAYSPAPVVT
CCEEEEHHHCCCCCCEEEEEECCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCCCCCH
DEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMIDKQGNPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCEECCCCEEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCEEE
LRMKSDLVELCLAACAGKLDEKTSEWDDRASLGVVVAAGGYPGSYNTGDEIYGLPQQEVA
EEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEECCCHHHHC
DGKVFHAGTKLSDDQRVVTNGGRVLCVTALGDSVAQAQQRAYQLLTDIRWDGSFSRSDIG
CCEEEECCCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
WRAIEREKANG
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]