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The map label for this gene is intB [H]

Identifier: 238028380

GI number: 238028380

Start: 3222393

End: 3223655

Strand: Direct

Name: intB [H]

Synonym: bglu_1g28410

Alternate gene names: 238028380

Gene position: 3222393-3223655 (Clockwise)

Preceding gene: 238028379

Following gene: 238028389

Centisome position: 82.49

GC content: 60.65

Gene sequence:

>1263_bases
TTGGGGGCAACCCACCCGAAATTGCCTTACCCGTCAGGAGTTGCCCCCGTGCCATTAACCGATACAGCCATCCGGAACGC
CAAGCCTGCCGACAAGCCCTTGCGCCTGTTCGACGGAGGCGGCCTCTATCTGGAAATCGCCCCGAGCGGGGGCAAGTGGT
GGCGCCTCAAATACCGGTTTGGGGGCAAGGAAAAGCGCTACTCGCTCGGCGTCTACCCGGACGTTACATTGGCGATCGCA
CGGAAGAAGCGCGACGAAGCCCGTGAAAAGCTCGCTGCCGGCATCGATCCCGGTGAAGCGAAGAAGGCGGAAAAAAGGGC
CGGCCTGCTTGCGGCCGCCCATTCGTTTGAGGTTGTCGCTCGCGGCTGGATGGCCGAGCGCAAGACGACTGTAGAGCCAG
CGCAGCATGACAAGACACTCGCCCGCATGGAGAACGATGTATTCCCATGGCTTGGCAAGCGCCCTATCGCTGAAATCGAC
GCCCCGGAGATACTGGTTGTACTGAAGCGCGTAGACGGGCGTGGTGCCAGATTTACCGCCCATCGCATCCGCAGCGAGAT
TAGCCGGGTATTCCGCTACGGCATCAAAGAGGGGCACTGCAAGGCCGATCCAGCCCGAGATTTGGTAGATGCCATTCCGC
CCGCCCAGACAACGCACTTTGCCTCGATCACCGAGCCCGAGAAAGTTGCCGAGATGCTTCGCGCGTTCGACGGCTTCACG
GGTACTTTCCCGGTTCTCTGCGCGCTCAGACTAGCCCCGATGCTGTTCGTCCGCCCAGGCGAATTACGCAAGGCGCAGTG
GTCACAATTCGAGCTCGACAAAGGCGAGTGGCGCTATTTCGTCAACAAAACGAAAACGGATCACCTTGTTCCGCTGGCCA
CACAGGCGGTTGCGATTTTGCGCGAGCTTCACGCCCTGACTGGCGATGGCGTCTACGTGTTCCCCGGTGCCCGCGACCGA
AATCGCCCGATGAGCGAGGCCGCTATCAATGCAGCCCTTCGTCGCCTCGGCTACGACACTCGCACCGAGATTACCGGTCA
CGGCTTCCGGGCGATGGCACGGACGATCCTGCATGAAGAGCTGGAGGAAAAGCCGGAAGTGATCGAGCACCAACTAGCAC
ATACCGTCCCTGACAGCCTTGGGCGGGCGTACAACCGAACAAAATTCATCAAGGCCCGACGTTCGATGATGCAACAGTGG
GCGGACTACCTCGACAAGCTCAAAGCAGGGGCGGAAATCATTCCGATCGCGGCGGCCGGATAG

Upstream 100 bases:

>100_bases
GAAGTCCTAAAACCCGCGACAGCTCTAGGCTTCGCGGGTTTTTTCATTCCGGCAAAGTGCCGTGTGGTGCGTAGACAGCC
GGGGGCAAGTGGGGGCAACA

Downstream 100 bases:

>100_bases
ACCCGTGCCGCCACGCGTATGACCCCCTTGTCACGCCCCTCCCACCATTTTCCAGTACTTGGTCAGACGCTACCGACTCC
CTGCATTTCGGTATGAATAT

Product: phage integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MGATHPKLPYPSGVAPVPLTDTAIRNAKPADKPLRLFDGGGLYLEIAPSGGKWWRLKYRFGGKEKRYSLGVYPDVTLAIA
RKKRDEAREKLAAGIDPGEAKKAEKRAGLLAAAHSFEVVARGWMAERKTTVEPAQHDKTLARMENDVFPWLGKRPIAEID
APEILVVLKRVDGRGARFTAHRIRSEISRVFRYGIKEGHCKADPARDLVDAIPPAQTTHFASITEPEKVAEMLRAFDGFT
GTFPVLCALRLAPMLFVRPGELRKAQWSQFELDKGEWRYFVNKTKTDHLVPLATQAVAILRELHALTGDGVYVFPGARDR
NRPMSEAAINAALRRLGYDTRTEITGHGFRAMARTILHEELEEKPEVIEHQLAHTVPDSLGRAYNRTKFIKARRSMMQQW
ADYLDKLKAGAEIIPIAAAG

Sequences:

>Translated_420_residues
MGATHPKLPYPSGVAPVPLTDTAIRNAKPADKPLRLFDGGGLYLEIAPSGGKWWRLKYRFGGKEKRYSLGVYPDVTLAIA
RKKRDEAREKLAAGIDPGEAKKAEKRAGLLAAAHSFEVVARGWMAERKTTVEPAQHDKTLARMENDVFPWLGKRPIAEID
APEILVVLKRVDGRGARFTAHRIRSEISRVFRYGIKEGHCKADPARDLVDAIPPAQTTHFASITEPEKVAEMLRAFDGFT
GTFPVLCALRLAPMLFVRPGELRKAQWSQFELDKGEWRYFVNKTKTDHLVPLATQAVAILRELHALTGDGVYVFPGARDR
NRPMSEAAINAALRRLGYDTRTEITGHGFRAMARTILHEELEEKPEVIEHQLAHTVPDSLGRAYNRTKFIKARRSMMQQW
ADYLDKLKAGAEIIPIAAAG
>Mature_419_residues
GATHPKLPYPSGVAPVPLTDTAIRNAKPADKPLRLFDGGGLYLEIAPSGGKWWRLKYRFGGKEKRYSLGVYPDVTLAIAR
KKRDEAREKLAAGIDPGEAKKAEKRAGLLAAAHSFEVVARGWMAERKTTVEPAQHDKTLARMENDVFPWLGKRPIAEIDA
PEILVVLKRVDGRGARFTAHRIRSEISRVFRYGIKEGHCKADPARDLVDAIPPAQTTHFASITEPEKVAEMLRAFDGFTG
TFPVLCALRLAPMLFVRPGELRKAQWSQFELDKGEWRYFVNKTKTDHLVPLATQAVAILRELHALTGDGVYVFPGARDRN
RPMSEAAINAALRRLGYDTRTEITGHGFRAMARTILHEELEEKPEVIEHQLAHTVPDSLGRAYNRTKFIKARRSMMQQWA
DYLDKLKAGAEIIPIAAAG

Specific function: Unknown

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=401, Percent_Identity=33.9152119700748, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI145693166, Length=401, Percent_Identity=34.9127182044888, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1788690, Length=395, Percent_Identity=34.9367088607595, Blast_Score=214, Evalue=6e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46912; Mature: 46780

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGATHPKLPYPSGVAPVPLTDTAIRNAKPADKPLRLFDGGGLYLEIAPSGGKWWRLKYRF
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCEEEEEEEE
GGKEKRYSLGVYPDVTLAIARKKRDEAREKLAAGIDPGEAKKAEKRAGLLAAAHSFEVVA
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RGWMAERKTTVEPAQHDKTLARMENDVFPWLGKRPIAEIDAPEILVVLKRVDGRGARFTA
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHEEECCCCCCCHHHH
HRIRSEISRVFRYGIKEGHCKADPARDLVDAIPPAQTTHFASITEPEKVAEMLRAFDGFT
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
GTFPVLCALRLAPMLFVRPGELRKAQWSQFELDKGEWRYFVNKTKTDHLVPLATQAVAIL
CHHHHHHHHHHHHHHEECCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
RELHALTGDGVYVFPGARDRNRPMSEAAINAALRRLGYDTRTEITGHGFRAMARTILHEE
HHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LEEKPEVIEHQLAHTVPDSLGRAYNRTKFIKARRSMMQQWADYLDKLKAGAEIIPIAAAG
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
>Mature Secondary Structure 
GATHPKLPYPSGVAPVPLTDTAIRNAKPADKPLRLFDGGGLYLEIAPSGGKWWRLKYRF
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCEEEEEEEE
GGKEKRYSLGVYPDVTLAIARKKRDEAREKLAAGIDPGEAKKAEKRAGLLAAAHSFEVVA
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RGWMAERKTTVEPAQHDKTLARMENDVFPWLGKRPIAEIDAPEILVVLKRVDGRGARFTA
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHEEECCCCCCCHHHH
HRIRSEISRVFRYGIKEGHCKADPARDLVDAIPPAQTTHFASITEPEKVAEMLRAFDGFT
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
GTFPVLCALRLAPMLFVRPGELRKAQWSQFELDKGEWRYFVNKTKTDHLVPLATQAVAIL
CHHHHHHHHHHHHHHEECCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHH
RELHALTGDGVYVFPGARDRNRPMSEAAINAALRRLGYDTRTEITGHGFRAMARTILHEE
HHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LEEKPEVIEHQLAHTVPDSLGRAYNRTKFIKARRSMMQQWADYLDKLKAGAEIIPIAAAG
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]